Restriction map for
pFN21ASDA1542
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# NO CUT ENZYMES ( 64 enzymes) #
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AclI AcsI AflII AgeI AhdI
Aor51HI ApoI AscI AseI AsnI
Asp700I AspEI AvrII BclI BfrI
BlnI BsaBI Bsp1407I BspDI BspHI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI DraI DraIII Eam1105I Eco47III
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI MamI MfeI MluI
MunI NdeI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI ScaI
SfuI SgrAI SnaBI SpeI SphI
SspBI SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 3261 GACGTC GACGT^C
Acc65I 620 GGTACC G^GTACC
Alw44I 2227 GTGCAC G^TGCAC
AocI 4150 CCTGAGG CC^T[ACGT]AGG
ApaI 3237 GGGCCC GGGCC^C
ApaLI 2227 GTGCAC G^TGCAC
Asp718I 620 GGTACC G^GTACC
AspI 3558 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1320 GGATCC G^GATCC
BbrPI 4372 CACGTG CAC^GTG
BciVI 2042 GGATAC GTATCC(6/5)
BsiWI 4865 CGTACG C^GTACG
BspLU11I 3678 ACATGT A^CATGT
BsrDI 245 CATTGC GCAATG(2/0)
BssSI 3427 CACGAG CACGAG(-5/-1)
Bsu36I 4150 CCTGAGG CC^T[ACGT]AGG
CpoI 2688 CGGACCG CG^G[AT]CCG
EagI 3701 CGGCCG C^GGCCG
EclXI 3701 CGGCCG C^GGCCG
Eco52I 3701 CGGCCG C^GGCCG
Eco81I 4150 CCTGAGG CC^T[ACGT]AGG
HincII 2313 GTCGAC GT[CT]^[GA]AC
HindII 2313 GTCGAC GT[CT]^[GA]AC
KpnI 620 GGTACC GGTAC^C
NaeI 3152 GCCGGC GCC^GGC
NcoI 2265 CCATGG C^CATGG
NgoMIV 3152 GCCGGC G^CCGGC
NheI 4647 GCTAGC G^CTAGC
PflFI 3558 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4372 CACGTG CAC^GTG
PmlI 4372 CACGTG CAC^GTG
RsrII 2688 CGGACCG CG^G[AT]CCG
SalI 2313 GTCGAC G^TCGAC
SspI 2338 AATATT AAT^ATT
StuI 1201 AGGCCT AGG^CCT
Tth111I 3558 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccI 2313 GTCGAC GT^[AC][GT]AC
2485 GTCTAC
BbvCI 1590 CCTCAGC CCTCAGC(-5/-2)
4063 GCTGAGG
BcgI 2465 CGAAGCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3676 GCACATGTCTCG
BfaI 4525 CTAG C^TAG
4648 CTAG
BlpI 1088 GCTCAGC GC^T[ACGT]AGC
1746 GCTCAGC
Bpu1102I 1088 GCTCAGC GC^T[ACGT]AGC
1746 GCTCAGC
BsaAI 4372 CACGTG [CT]AC^GT[GA]
4867 TACGTG
BsmBI 1434 CGTCTC CGTCTC(1/5)
1380 GAGACG
CelII 1088 GCTCAGC GC^T[ACGT]AGC
1746 GCTCAGC
DrdI 397 GACTGCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3261 GACGTCCCGGTC
KspI 1469 CCGCGG CCGC^GG
1784 CCGCGG
MaeI 4525 CTAG C^TAG
4648 CTAG
MseI 455 TTAA T^TAA
496 TTAA
MslI 148 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
963 CACTGCCGTG
PaeR7I 794 CTCGAG C^TCGAG
3087 CTCGAG
SacII 1469 CCGCGG CCGC^GG
1784 CCGCGG
SapI 1339 GAAGAGC GCTCTTC(1/4)
3293 GCTCTTC
SbfI 3717 CCTGCAGG CCTGCA^GG
4218 CCTGCAGG
SexAI 3652 ACCAGGT A^CC[AT]GGT
4853 ACCTGGT
SfiI 3238 GGCCCCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3702 GGCCGACAAGGCC
Sse8387I 3717 CCTGCAGG CCTGCA^GG
4218 CCTGCAGG
Tru9I 455 TTAA T^TAA
496 TTAA
XhoI 794 CTCGAG C^TCGAG
3087 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AspHI
AvaI AvaII AviII BalI BanI
BanII BbeI BbsI BbvI BcnI
BglI BglII BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI