(Circular) (Six-base) MAPSORT of: pFN21ASDA1542.seq Check: 3542 from: 1 to: 9676 pFN21ASDA1542 9676 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2096 2096 Size: 9676 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5288 7486 278 Size: 53 83 186 936 3752 2198 2468 Fragments arranged by size: 3752 2468 2198 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4337 4509 7019 1937 Size: 2400 172 2510 4594 Fragments arranged by size: 4594 2510 2400 172 Acc65I G'GTAC_C Cuts at: 2643 6997 2643 Size: 4354 5322 AclI AA'CG_TT Cuts at: 1563 7803 8176 1563 Size: 6240 373 3063 Fragments arranged by size: 6240 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2271 3877 4043 5957 6515 7739 9123 9391 Size: 639 1606 166 1914 558 1224 1384 268 Cuts at: 9391 1632 Size: 1917 Fragments arranged by size: 1917 1914 1606 1384 1224 639 558 268 166 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9479 AflIII A'CryG_T Cuts at: 1203 4416 5701 5827 8575 9552 1203 Size: 3213 1285 126 2748 977 1327 Fragments arranged by size: 3213 2748 1327 1285 977 126 AgeI A'CCGG_T Cuts at: 8507 8507 Size: 9676 AhdI GACnn_n'nnGTC Cuts at: 8405 8405 Size: 9676 AleI CACnn'nnGTG Cuts at: 1166 2990 1166 Size: 1824 7852 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2359 4022 4247 6744 6836 1184 Size: 1175 1663 225 2497 92 4024 Fragments arranged by size: 4024 2497 1663 1175 225 92 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2681 2713 2879 2911 6398 6399 6430 6431 2681 Size: 32 166 32 3487 1 31 1 5926 Fragments arranged by size: 5926 3487 166 32 32 31 1 1 AlwNI CAG_nnn'CTG Cuts at: 3240 3530 3845 3912 4149 4751 4804 6574 7419 Size: 290 315 67 237 602 53 1770 845 Cuts at: 7419 8991 3240 Size: 1572 3925 Fragments arranged by size: 3925 1770 1572 845 602 315 290 237 67 53 ApaI G_GGCC'C Cuts at: 5264 5264 Size: 9676 ApaLI G'TGCA_C Cuts at: 4250 7733 8889 4250 Size: 3483 1156 5037 Fragments arranged by size: 5037 3483 1156 ApoI r'AATT_y Cuts at: 1493 6985 7211 7363 9472 1493 Size: 5492 226 152 2109 1697 Fragments arranged by size: 5492 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 8230 160 Size: 872 7198 1606 Fragments arranged by size: 7198 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9676 AvaI C'yCGr_G Cuts at: 1870 1943 2817 2832 2933 3705 4236 4244 4562 Size: 73 874 15 101 772 531 8 318 Cuts at: 4562 5110 5376 5487 6285 6315 6410 7001 1870 Size: 548 266 111 798 30 95 591 4545 Fragments arranged by size: 4545 874 798 772 591 548 531 318 266 111 101 95 73 30 15 8 BamHI G'GATC_C Cuts at: 3343 7006 7373 3343 Size: 3663 367 5646 Fragments arranged by size: 5646 3663 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2643 2680 2791 2928 3480 Size: 325 217 627 856 37 111 137 552 Cuts at: 3480 3496 3814 3923 4006 4178 4231 6041 6091 Size: 16 318 109 83 172 53 1810 50 Cuts at: 6091 6112 6289 6997 8452 618 Size: 21 177 708 1455 1842 Fragments arranged by size: 1842 1810 1455 856 708 627 552 325 318 217 177 172 137 111 109 83 53 50 37 21 16 BanII G_rGCy'C Cuts at: 1391 2063 2500 3113 3273 3412 3728 3771 4245 Size: 672 437 613 160 139 316 43 474 Cuts at: 4245 4698 4788 4842 4983 5111 5264 5309 5885 Size: 453 90 54 141 128 153 45 576 Cuts at: 5885 6217 6283 6995 1391 Size: 332 66 712 4072 Fragments arranged by size: 4072 712 672 613 576 474 453 437 332 316 160 153 141 139 128 90 66 54 45 43 BbeI G_GCGC'C Cuts at: 2932 3484 3818 6116 2932 Size: 552 334 2298 6492 Fragments arranged by size: 6492 2298 552 334 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2199 2223 2232 2259 2931 3047 3055 Size: 971 300 24 9 27 672 116 8 Cuts at: 3055 4490 5670 6015 6135 6659 928 Size: 1435 1180 345 120 524 3945 Fragments arranged by size: 3945 1435 1180 971 672 524 345 300 120 116 27 24 9 8 BbvCI CC'TCA_GC Cuts at: 3614 6087 3614 Size: 2473 7203 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4477 4511 5688 5722 7867 7901 1903 Size: 34 2540 34 1177 34 2145 34 3678 Fragments arranged by size: 3678 2540 2145 1177 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4059 7568 8778 4059 Size: 3509 1210 4957 Fragments arranged by size: 4957 3509 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9676 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2963 3626 3823 Size: 107 122 71 631 605 1291 663 197 Cuts at: 3823 3848 4760 5268 5732 5963 6287 8287 136 Size: 25 912 508 464 231 324 2000 1525 Fragments arranged by size: 2000 1525 1291 912 663 631 605 508 464 324 231 197 122 107 71 25 BglII A'GATC_T Cuts at: 2231 3122 3966 6828 9671 2231 Size: 891 844 2862 2843 2236 Fragments arranged by size: 2862 2843 2236 891 844 BlpI GC'TnA_GC Cuts at: 3112 3770 7093 3112 Size: 658 3323 5695 Fragments arranged by size: 5695 3323 658 Bme1580I G_kGCm'C Cuts at: 1790 2404 2796 4181 4236 4254 5264 5539 6044 Size: 614 392 1385 55 18 1010 275 505 Cuts at: 6044 6292 6588 7737 8893 1790 Size: 248 296 1149 1156 2573 Fragments arranged by size: 2573 1385 1156 1149 1010 614 505 392 296 275 248 55 18 BmgBI CAC'GTC Cuts at: 1318 4948 5830 1318 Size: 3630 882 5164 Fragments arranged by size: 5164 3630 882 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2525 4137 4316 Size: 416 53 83 399 24 1108 1612 179 Cuts at: 4316 6090 6384 6575 7407 8365 442 Size: 1774 294 191 832 958 1753 Fragments arranged by size: 1774 1753 1612 1108 958 832 416 399 294 191 179 83 53 24 BmtI G_CTAG'C Cuts at: 1056 6674 1056 Size: 5618 4058 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2785 3426 3648 4477 4780 2785 Size: 641 222 829 303 7681 Fragments arranged by size: 7681 829 641 303 222 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2294 2657 3618 4381 4525 5170 5182 5429 Size: 1055 363 961 763 144 645 12 247 Cuts at: 5429 5780 8336 1239 Size: 351 2556 2579 Fragments arranged by size: 2579 2556 1055 961 763 645 363 351 247 144 12 Bpu10I CC'TnA_GC Cuts at: 2981 3614 4982 6062 6087 2981 Size: 633 1368 1080 25 6570 Fragments arranged by size: 6570 1368 1080 633 25 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3955 5830 6463 7801 8666 8964 3955 Size: 1875 633 1338 865 298 4667 Fragments arranged by size: 4667 1875 1338 865 633 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2364 2773 5484 8339 882 Size: 617 865 409 2711 2855 2219 Fragments arranged by size: 2855 2711 2219 865 617 409 BsaAI yAC'GTr Cuts at: 493 1204 6397 6892 6973 7467 493 Size: 711 5193 495 81 494 2702 Fragments arranged by size: 5193 2702 711 495 494 81 BsaBI GATnn'nnATC Cuts at: 9670 9670 Size: 9676 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2204 2387 2929 3011 Size: 53 83 186 936 671 183 542 82 Cuts at: 3011 3481 3815 4862 5285 5853 6113 7483 7865 Size: 470 334 1047 423 568 260 1370 382 Cuts at: 7865 8500 275 Size: 635 1451 Fragments arranged by size: 1451 1370 1047 936 671 635 568 542 470 423 382 334 260 186 183 83 82 53 BsaWI w'CCGG_w Cuts at: 2883 2889 3086 4353 5493 6403 8109 8507 8781 Size: 6 197 1267 1140 910 1706 398 274 Cuts at: 8781 8928 9339 2883 Size: 147 411 3220 Fragments arranged by size: 3220 1706 1267 1140 910 411 398 274 197 147 6 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2587 2617 2879 2909 1178 Size: 30 165 30 1184 30 262 30 7945 Fragments arranged by size: 7945 1184 262 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2225 2570 2573 2576 2774 2777 3530 3533 Size: 1039 345 3 3 198 3 753 3 Cuts at: 3533 4796 4865 4904 5153 6191 6662 6710 6752 Size: 1263 69 39 249 1038 471 48 42 Cuts at: 6752 6806 6956 1186 Size: 54 150 3906 Fragments arranged by size: 3906 1263 1039 1038 753 471 345 249 198 150 69 54 48 42 39 3 3 3 3 BseYI C'CCAG_C Cuts at: 3211 3538 3574 4273 4280 4937 5629 5953 6471 Size: 327 36 699 7 657 692 324 518 Cuts at: 6471 6601 8879 9375 3211 Size: 130 2278 496 3512 Fragments arranged by size: 3512 2278 699 692 657 518 496 327 324 130 36 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4661 4764 6698 6842 1163 Size: 573 2925 103 1934 144 3997 Fragments arranged by size: 3997 2925 1934 573 144 103 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2602 5294 5300 5727 7126 7887 Size: 275 186 601 2692 6 427 1399 761 Cuts at: 7887 8915 1540 Size: 1028 2301 Fragments arranged by size: 2692 2301 1399 1028 761 601 427 275 186 6 BsiHKAI G_wGCw'C Cuts at: 2500 2987 3113 3712 3764 4254 4983 5064 5111 Size: 487 126 599 52 490 729 81 47 Cuts at: 5111 5253 5331 5393 5532 5547 5885 7737 7822 Size: 142 78 62 139 15 338 1852 85 Cuts at: 7822 8893 2500 Size: 1071 3283 Fragments arranged by size: 3283 1852 1071 729 599 490 487 338 142 139 126 85 81 78 62 52 47 15 BsiWI C'GTAC_G Cuts at: 6888 6888 Size: 9676 BsmI GAATG_Cn' Cuts at: 2363 2449 2660 7187 7280 2363 Size: 86 211 4527 93 4759 Fragments arranged by size: 4759 4527 211 93 86 BsmBI CGTCTCn'nnnn_ Cuts at: 3397 3463 3397 Size: 66 9610 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2404 2500 2796 2987 3113 3273 Size: 399 273 341 96 296 191 126 160 Cuts at: 3273 3412 3712 3728 3764 3771 4181 4236 4245 Size: 139 300 16 36 7 410 55 9 Cuts at: 4245 4254 4698 4788 4842 4983 5064 5111 5253 Size: 9 444 90 54 141 81 47 142 Cuts at: 5253 5264 5309 5331 5393 5532 5539 5547 5885 Size: 11 45 22 62 139 7 8 338 Cuts at: 5885 6044 6217 6283 6292 6588 6995 7737 7822 Size: 159 173 66 9 296 407 742 85 Cuts at: 7822 8893 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 444 410 407 399 341 338 300 296 296 273 191 173 160 159 142 141 139 139 126 96 90 85 81 66 62 55 54 47 45 36 22 16 11 9 9 9 8 7 7 BspEI T'CCGG_A Cuts at: 3086 4353 6403 9339 3086 Size: 1267 2050 2936 3423 Fragments arranged by size: 3423 2936 2050 1267 BspHI T'CATG_A Cuts at: 7565 9295 7565 Size: 1730 7946 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2096 2813 3543 3774 6249 7031 844 Size: 1252 717 730 231 2475 782 3489 Fragments arranged by size: 3489 2475 1252 782 730 717 231 BsrBI CCG'CTC Cuts at: 1394 1823 3293 4894 5004 5196 5455 7563 1394 Size: 429 1470 1601 110 192 259 2108 3507 Fragments arranged by size: 3507 2108 1601 1470 429 259 192 110 BsrDI GCAATG_nn' Cuts at: 65 1248 2267 8171 8345 65 Size: 1183 1019 5904 174 1396 Fragments arranged by size: 5904 1396 1183 1019 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2683 4529 5175 8320 8507 1700 Size: 110 873 1846 646 3145 187 2869 Fragments arranged by size: 3145 2869 1846 873 646 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9676 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9676 BssSI C'ACGA_G Cuts at: 5450 7736 8748 5450 Size: 2286 1012 6378 Fragments arranged by size: 6378 2286 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3940 4648 4751 794 Size: 3146 708 103 5719 Fragments arranged by size: 5719 3146 708 103 BstBI TT'CG_AA Cuts at: 2007 8480 2007 Size: 6473 3203 BstEII G'GTnAC_C Cuts at: 1184 4900 6229 6781 1184 Size: 3716 1329 552 4079 Fragments arranged by size: 4079 3716 1329 552 BstXI CCAn_nnnn'nTGG Cuts at: 4842 5407 6217 4842 Size: 565 810 8301 Fragments arranged by size: 8301 810 565 BstYI r'GATC_y Cuts at: 1972 2231 2526 2892 3122 3328 3343 3966 4137 Size: 259 295 366 230 206 15 623 171 Cuts at: 4137 6828 7006 7373 7758 7775 9227 9313 9325 Size: 2691 178 367 385 17 1452 86 12 Cuts at: 9325 9671 1972 Size: 346 1977 Fragments arranged by size: 2691 1977 1452 623 385 367 366 346 295 259 230 206 178 171 86 17 15 12 Bsu36I CC'TnA_GG Cuts at: 6174 6174 Size: 9676 BtgI C'CryG_G Cuts at: 513 1065 2022 2379 2898 3444 3462 3492 3807 Size: 552 957 357 519 546 18 30 315 Cuts at: 3807 4120 4288 5814 5967 6513 513 Size: 313 168 1526 153 546 3676 Fragments arranged by size: 3676 1526 957 552 546 546 519 357 315 313 168 153 30 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2649 487 Size: 683 56 1423 7514 Fragments arranged by size: 7514 1423 683 56 BtsI GCAGTG_nn' Cuts at: 2143 2985 3364 3589 3710 4006 4752 6627 7192 Size: 842 379 225 121 296 746 1875 565 Cuts at: 7192 7986 8006 2143 Size: 794 20 3813 Fragments arranged by size: 3813 1875 842 794 746 565 379 296 225 121 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9676 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9641 DraI TTT'AAA Cuts at: 6980 6980 Size: 9676 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2426 5290 8683 809 Size: 507 1110 2864 3393 1802 Fragments arranged by size: 3393 2864 1802 1110 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2901 3447 5456 Size: 54 1425 50 275 18 1071 546 2009 Cuts at: 5456 5573 5724 5964 5970 6750 6870 7123 8012 Size: 117 151 240 6 780 120 253 889 Cuts at: 8012 9641 8 Size: 1629 43 Fragments arranged by size: 2009 1629 1425 1071 889 780 546 275 253 240 151 120 117 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 5724 7123 1537 Size: 275 3912 1399 4090 Fragments arranged by size: 4090 3912 1399 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3029 3146 3151 3357 4475 5039 5323 5917 Size: 1676 117 5 206 1118 564 284 594 Cuts at: 5917 6767 7606 1353 Size: 850 839 3423 Fragments arranged by size: 3423 1676 1118 850 839 594 564 284 206 117 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4711 8263 8637 8783 387 Size: 1064 138 3122 3552 374 146 1280 Fragments arranged by size: 3552 3122 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2498 3111 4981 5109 5883 2498 Size: 613 1870 128 774 6291 Fragments arranged by size: 6291 1870 774 613 128 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2271 2294 2657 3618 3877 4043 4381 Size: 393 639 23 363 961 259 166 338 Cuts at: 4381 4525 5170 5182 5429 5780 5957 6515 7739 Size: 144 645 12 247 351 177 558 1224 Cuts at: 7739 8336 9123 9391 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1224 961 787 645 639 597 558 393 363 351 338 268 259 247 177 166 144 23 12 EcoNI CCTnn'n_nnAGG Cuts at: 2820 5642 6035 6812 6881 2820 Size: 2822 393 777 69 5615 Fragments arranged by size: 5615 2822 777 393 69 EcoO109I rG'GnC_Cy Cuts at: 2566 2747 2829 3786 4013 4761 4801 4986 5260 Size: 181 82 957 227 748 40 185 274 Cuts at: 5260 5261 5508 5734 2566 Size: 1 247 226 6508 Fragments arranged by size: 6508 957 748 274 247 227 226 185 181 82 40 1 EcoRI G'AATT_C Cuts at: 6985 7363 6985 Size: 378 9298 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3955 3955 Size: 9676 FspI TGC'GCA Cuts at: 3221 6496 6812 6914 8182 3221 Size: 3275 316 102 1268 4715 Fragments arranged by size: 4715 3275 1268 316 102 HaeII r_GCGC'y Cuts at: 1678 2932 3004 3484 3621 3818 4034 4548 5146 Size: 1254 72 480 137 197 216 514 598 Cuts at: 5146 5636 6116 6264 8823 1678 Size: 490 480 148 2559 2531 Fragments arranged by size: 2559 2531 1254 598 514 490 480 480 216 197 148 137 72 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2189 2221 4847 4879 5093 Size: 32 135 32 617 32 2626 32 214 Cuts at: 5093 5125 5828 5860 5867 5899 6122 6154 8317 Size: 32 703 32 7 32 223 32 2163 Cuts at: 8317 8349 8391 8423 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2626 2163 703 617 223 214 135 42 32 32 32 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 4338 7020 7266 669 Size: 988 281 2400 2682 246 3079 Fragments arranged by size: 3079 2682 2400 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9676 HpaI GTT'AAC Cuts at: 7266 7266 Size: 9676 KasI G'GCGC_C Cuts at: 2928 3480 3814 6112 2928 Size: 552 334 2298 6492 Fragments arranged by size: 6492 2298 552 334 KpnI G_GTAC'C Cuts at: 2647 7001 2647 Size: 4354 5322 MfeI C'AATT_G Cuts at: 7275 7275 Size: 9676 MluI A'CGCG_T Cuts at: 9552 9552 Size: 9676 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4545 4560 6231 8790 8974 1305 Size: 3240 15 1671 2559 184 2007 Fragments arranged by size: 3240 2559 2007 1671 184 15 MscI TGG'CCA Cuts at: 10 64 1489 1832 3449 6752 6872 10 Size: 54 1425 343 1617 3303 120 2814 Fragments arranged by size: 3303 2814 1617 1425 343 120 54 MslI CAynn'nnrTG Cuts at: 518 1166 2175 2990 7634 7993 8152 518 Size: 648 1009 815 4644 359 159 2042 Fragments arranged by size: 4644 2042 1009 815 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2309 2738 3240 3494 3774 3809 4495 5158 Size: 703 429 502 254 280 35 686 663 Cuts at: 5158 5437 5771 6590 7092 7769 8917 9162 1606 Size: 279 334 819 502 677 1148 245 2120 Fragments arranged by size: 2120 1148 819 703 686 677 663 502 502 429 334 280 279 254 245 35 NaeI GCC'GGC Cuts at: 5177 5177 Size: 9676 NarI GG'CG_CC Cuts at: 2929 3481 3815 6113 2929 Size: 552 334 2298 6492 Fragments arranged by size: 6492 2298 552 334 NcoI C'CATG_G Cuts at: 513 1065 2022 4288 513 Size: 552 957 2266 5901 Fragments arranged by size: 5901 2266 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9676 NgoMIV G'CCGG_C Cuts at: 5175 5175 Size: 9676 NheI G'CTAG_C Cuts at: 1052 6670 1052 Size: 5618 4058 NotI GC'GGCC_GC Cuts at: 7123 7123 Size: 9676 NspI r_CATG'y Cuts at: 1132 1229 5705 6721 6913 7034 8579 1132 Size: 97 4476 1016 192 121 1545 2229 Fragments arranged by size: 4476 2229 1545 1016 192 121 97 PasI CC'CwG_GG Cuts at: 1348 2063 3845 4842 5265 1348 Size: 715 1782 997 423 5759 Fragments arranged by size: 5759 1782 997 715 423 PciI A'CATG_T Cuts at: 5701 8575 5701 Size: 2874 6802 PflMI CCAn_nnn'nTGG Cuts at: 1108 2380 3445 4804 1108 Size: 1272 1065 1359 5980 Fragments arranged by size: 5980 1359 1272 1065 PfoI T'CCnGG_A Cuts at: 2673 3473 3719 4366 5757 2673 Size: 800 246 647 1391 6592 Fragments arranged by size: 6592 1391 800 647 246 PmeI GTTT'AAAC Cuts at: 6980 6980 Size: 9676 PmlI CAC'GTG Cuts at: 6397 6397 Size: 9676 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2879 2911 4692 4724 7745 7777 9258 9290 2879 Size: 32 1781 32 3021 32 1481 32 3265 Fragments arranged by size: 3265 3021 1781 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2566 2747 2829 3786 5508 2566 Size: 181 82 957 1722 6734 Fragments arranged by size: 6734 1722 957 181 82 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9676 PsiI TTA'TAA Cuts at: 7246 7246 Size: 9676 PspOMI G'GGCC_C Cuts at: 5260 5260 Size: 9676 PspXI vC'TCGA_Gb Cuts at: 2817 5110 2817 Size: 2293 7383 PstI C_TGCA'G Cuts at: 830 3432 3702 4956 5423 5745 6246 6634 7028 Size: 2602 270 1254 467 322 501 388 394 Cuts at: 7028 830 Size: 3478 Fragments arranged by size: 3478 2602 1254 501 467 394 388 322 270 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9676 PvuII CAG'CTG Cuts at: 3240 3774 4495 5771 3240 Size: 534 721 1276 7145 Fragments arranged by size: 7145 1276 721 534 RsrII CG'GwC_CG Cuts at: 4712 4712 Size: 9676 SacI G_AGCT'C Cuts at: 2500 3113 4983 5111 5885 2500 Size: 613 1870 128 774 6291 Fragments arranged by size: 6291 1870 774 613 128 SacII CC_GC'GG Cuts at: 3495 3810 3495 Size: 315 9361 SalI G'TCGA_C Cuts at: 1936 4336 7018 1936 Size: 2400 2682 4594 Fragments arranged by size: 4594 2682 2400 SanDI GG'GwC_CC Cuts at: 2747 2747 Size: 9676 SapI GCTCTTCn'nnn_ Cuts at: 3357 5323 3357 Size: 1966 7710 SbfI CC_TGCA'GG Cuts at: 5745 6246 7028 5745 Size: 501 782 8393 Fragments arranged by size: 8393 782 501 ScaI AGT'ACT Cuts at: 7924 9636 7924 Size: 1712 7964 SexAI A'CCwGG_T Cuts at: 5675 6876 5675 Size: 1201 8475 SfcI C'TryA_G Cuts at: 826 1045 3428 3698 4952 5419 5741 6242 6630 Size: 219 2383 270 1254 467 322 501 388 Cuts at: 6630 7024 8159 8840 9031 826 Size: 394 1135 681 191 1471 Fragments arranged by size: 2383 1471 1254 1135 681 501 467 394 388 322 270 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 5268 5732 5268 Size: 464 9212 SfoI GGC'GCC Cuts at: 2930 3482 3816 6114 2930 Size: 552 334 2298 6492 Fragments arranged by size: 6492 2298 552 334 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9676 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9676 SmaI CCC'GGG Cuts at: 1872 2834 4238 4246 6287 6412 7003 1872 Size: 962 1404 8 2041 125 591 4545 Fragments arranged by size: 4545 2041 1404 962 591 125 8 SmlI C'TyrA_G Cuts at: 820 1017 1943 2817 3970 5110 5845 6478 7780 Size: 197 926 874 1153 1140 735 633 1302 Cuts at: 7780 8681 8943 820 Size: 901 262 1553 Fragments arranged by size: 1553 1302 1153 1140 926 901 874 735 633 262 197 SnaBI TAC'GTA Cuts at: 493 6973 7467 493 Size: 6480 494 2702 Fragments arranged by size: 6480 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9676 SphI G_CATG'C Cuts at: 1132 7034 1132 Size: 5902 3774 SspI AAT'ATT Cuts at: 5 52 4363 7600 5 Size: 47 4311 3237 2081 Fragments arranged by size: 4311 3237 2081 47 StuI AGG'CCT Cuts at: 1517 3226 1517 Size: 1709 7967 StyI C'CwwG_G Cuts at: 513 1065 2022 3733 4288 5163 5232 5688 6786 Size: 552 957 1711 555 875 69 456 1098 Cuts at: 6786 7115 513 Size: 329 3074 Fragments arranged by size: 3074 1711 1098 957 875 555 552 456 329 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2094 4951 4979 5123 5547 6059 6109 Size: 1255 514 2857 28 144 424 512 50 Cuts at: 6109 7703 7720 7873 8058 8503 325 Size: 1594 17 153 185 445 1498 Fragments arranged by size: 2857 1594 1498 1255 514 512 445 424 185 153 144 50 28 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6699 6795 6900 Size: 274 80 33 51 1443 4722 96 105 Cuts at: 6900 7922 9634 96 Size: 1022 1712 138 Fragments arranged by size: 4722 1712 1443 1022 274 138 105 96 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2587 2619 5458 5490 9636 9668 2587 Size: 32 2839 32 4146 32 2595 Fragments arranged by size: 4146 2839 2595 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5584 1316 Size: 4268 5408 XbaI T'CTAG_A Cuts at: 7012 7012 Size: 9676 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2965 5404 5965 6842 2965 Size: 2439 561 877 5799 Fragments arranged by size: 5799 2439 877 561 XhoI C'TCGA_G Cuts at: 1943 2817 5110 1943 Size: 874 2293 6509 Fragments arranged by size: 6509 2293 874 XmaI C'CCGG_G Cuts at: 1870 2832 4236 4244 6285 6410 7001 1870 Size: 962 1404 8 2041 125 591 4545 Fragments arranged by size: 4545 2041 1404 962 591 125 8 XmnI GAAnn'nnTTC Cuts at: 7805 7805 Size: 9676 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5286 7484 276 Size: 53 83 186 936 3752 2198 2468 Fragments arranged by size: 3752 2468 2198 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PsrI SrfI SwaI