Restriction map for
pFN21ASDA1542
(Linear) (Six-base) MAPSORT of: KIAA1542.seq Check: 1883 from: 1 to: 4947
KIAA1542.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 73 4947
Size: 73 4874
AatII G_ACGT'C
Cuts at: 0 3265 4947
Size: 3265 1682
AccI GT'mk_AC
Cuts at: 0 2314 2486 4947
Size: 2314 172 2461
Fragments arranged by size:
2461 2314 172
Acc65I G'GTAC_C
Cuts at: 0 620 4947
Size: 620 4327
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 1854 2020 3934 4492 4947
Size: 248 1606 166 1914 558 455
Fragments arranged by size:
1914 1606 558 455 248 166
AflIII A'CryG_T
Cuts at: 0 2393 3678 3804 4947
Size: 2393 1285 126 1143
Fragments arranged by size:
2393 1285 1143 126
AleI CACnn'nnGTG
Cuts at: 0 967 4947
Size: 967 3980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 336 1999 2224 4721 4813 4947
Size: 336 1663 225 2497 92 134
Fragments arranged by size:
2497 1663 336 225 134 92
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 658 690 856 888 4375 4376 4407 4408
Size: 658 32 166 32 3487 1 31 1
Cuts at: 4408 4947
Size: 539
Fragments arranged by size:
3487 658 539 166 32 32 31 1
1
AlwNI CAG_nnn'CTG
Cuts at: 0 1217 1507 1822 1889 2126 2728 2781 4551
Size: 1217 290 315 67 237 602 53 1770
Cuts at: 4551 4947
Size: 396
Fragments arranged by size:
1770 1217 602 396 315 290 237 67
53
ApaI G_GGCC'C
Cuts at: 0 3241 4947
Size: 3241 1706
ApaLI G'TGCA_C
Cuts at: 0 2227 4947
Size: 2227 2720
AvaI C'yCGr_G
Cuts at: 0 794 809 910 1682 2213 2221 2539 3087
Size: 794 15 101 772 531 8 318 548
Cuts at: 3087 3353 3464 4262 4292 4387 4947
Size: 266 111 798 30 95 560
Fragments arranged by size:
798 794 772 560 548 531 318 266
111 101 95 30 15 8
BamHI G'GATC_C
Cuts at: 0 1320 4947
Size: 1320 3627
BanI G'GyrC_C
Cuts at: 0 620 657 768 905 1457 1473 1791 1900
Size: 620 37 111 137 552 16 318 109
Cuts at: 1900 1983 2155 2208 4018 4068 4089 4266 4947
Size: 83 172 53 1810 50 21 177 681
Fragments arranged by size:
1810 681 620 552 318 177 172 137
111 109 83 53 50 37 21 16
BanII G_rGCy'C
Cuts at: 0 40 477 1090 1250 1389 1705 1748 2222
Size: 40 437 613 160 139 316 43 474
Cuts at: 2222 2675 2765 2819 2960 3088 3241 3286 3862
Size: 453 90 54 141 128 153 45 576
Cuts at: 3862 4194 4260 4947
Size: 332 66 687
Fragments arranged by size:
687 613 576 474 453 437 332 316
160 153 141 139 128 90 66 54
45 43 40
BbeI G_GCGC'C
Cuts at: 0 909 1461 1795 4093 4947
Size: 909 552 334 2298 854
Fragments arranged by size:
2298 909 854 552 334
BbsI GAAGACnn'nnnn_
Cuts at: 0 176 200 209 236 908 1024 1032 2467
Size: 176 24 9 27 672 116 8 1435
Cuts at: 2467 3647 3992 4112 4636 4947
Size: 1180 345 120 524 311
Fragments arranged by size:
1435 1180 672 524 345 311 176 120
116 27 24 9 8
BbvCI CC'TCA_GC
Cuts at: 0 1591 4064 4947
Size: 1591 2473 883
Fragments arranged by size:
2473 1591 883
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2454 2488 3665 3699 4947
Size: 2454 34 1177 34 1248
Fragments arranged by size:
2454 1248 1177 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2036 4947
Size: 2036 2911
BglI GCCn_nnn'nGGC
Cuts at: 0 940 1603 1800 1825 2737 3245 3709 3940
Size: 940 663 197 25 912 508 464 231
Cuts at: 3940 4264 4947
Size: 324 683
Fragments arranged by size:
940 912 683 663 508 464 324 231
197 25
BglII A'GATC_T
Cuts at: 0 208 1099 1943 4805 4947
Size: 208 891 844 2862 142
Fragments arranged by size:
2862 891 844 208 142
BlpI GC'TnA_GC
Cuts at: 0 1089 1747 4947
Size: 1089 658 3200
Fragments arranged by size:
3200 1089 658
Bme1580I G_kGCm'C
Cuts at: 0 381 773 2158 2213 2231 3241 3516 4021
Size: 381 392 1385 55 18 1010 275 505
Cuts at: 4021 4269 4565 4947
Size: 248 296 382
Fragments arranged by size:
1385 1010 505 392 382 381 296 275
248 55 18
BmgBI CAC'GTC
Cuts at: 0 2925 3807 4947
Size: 2925 882 1140
Fragments arranged by size:
2925 1140 882
BmrI ACTGGGnnnn_n'
Cuts at: 0 502 2114 2293 4067 4361 4552 4947
Size: 502 1612 179 1774 294 191 395
Fragments arranged by size:
1774 1612 502 395 294 191 179
BmtI G_CTAG'C
Cuts at: 0 4651 4947
Size: 4651 296
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 762 1403 1625 2454 2757 4947
Size: 762 641 222 829 303 2190
Fragments arranged by size:
2190 829 762 641 303 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 634 1595 2358 2502 3147 3159 3406
Size: 271 363 961 763 144 645 12 247
Cuts at: 3406 3757 4947
Size: 351 1190
Fragments arranged by size:
1190 961 763 645 363 351 271 247
144 12
Bpu10I CC'TnA_GC
Cuts at: 0 958 1591 2959 4039 4064 4947
Size: 958 633 1368 1080 25 883
Fragments arranged by size:
1368 1080 958 883 633 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1932 3807 4440 4947
Size: 1932 1875 633 507
Fragments arranged by size:
1932 1875 633 507
BsaI GGTCTCn'nnnn_
Cuts at: 0 341 750 3461 4947
Size: 341 409 2711 1486
Fragments arranged by size:
2711 1486 409 341
BsaAI yAC'GTr
Cuts at: 0 4374 4869 4947
Size: 4374 495 78
Fragments arranged by size:
4374 495 78
BsaHI Gr'CG_yC
Cuts at: 0 181 364 906 988 1458 1792 2839 3262
Size: 181 183 542 82 470 334 1047 423
Cuts at: 3262 3830 4090 4947
Size: 568 260 857
Fragments arranged by size:
1047 857 568 542 470 423 334 260
183 181 82
BsaWI w'CCGG_w
Cuts at: 0 860 866 1063 2330 3470 4380 4947
Size: 860 6 197 1267 1140 910 567
Fragments arranged by size:
1267 1140 910 860 567 197 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 564 594 856 886 4947
Size: 564 30 262 30 4061
Fragments arranged by size:
4061 564 262 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 202 547 550 553 751 754 1507 1510
Size: 202 345 3 3 198 3 753 3
Cuts at: 1510 2773 2842 2881 3130 4168 4639 4687 4729
Size: 1263 69 39 249 1038 471 48 42
Cuts at: 4729 4783 4933 4947
Size: 54 150 14
Fragments arranged by size:
1263 1038 753 471 345 249 202 198
150 69 54 48 42 39 14 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 1188 1515 1551 2250 2257 2914 3606 3930
Size: 1188 327 36 699 7 657 692 324
Cuts at: 3930 4448 4578 4947
Size: 518 130 369
Fragments arranged by size:
1188 699 692 657 518 369 327 324
130 36 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2638 2741 4675 4819 4947
Size: 2638 103 1934 144 128
Fragments arranged by size:
2638 1934 144 128 103
BsiEI CG_ry'CG
Cuts at: 0 579 3271 3277 3704 4947
Size: 579 2692 6 427 1243
Fragments arranged by size:
2692 1243 579 427 6
BsiHKAI G_wGCw'C
Cuts at: 0 477 964 1090 1689 1741 2231 2960 3041
Size: 477 487 126 599 52 490 729 81
Cuts at: 3041 3088 3230 3308 3370 3509 3524 3862 4947
Size: 47 142 78 62 139 15 338 1085
Fragments arranged by size:
1085 729 599 490 487 477 338 142
139 126 81 78 62 52 47 15
BsiWI C'GTAC_G
Cuts at: 0 4865 4947
Size: 4865 82
BsmI GAATG_Cn'
Cuts at: 0 340 426 637 4947
Size: 340 86 211 4310
Fragments arranged by size:
4310 340 211 86
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1374 1440 4947
Size: 1374 66 3507
Fragments arranged by size:
3507 1374 66
Bsp1286I G_dGCh'C
Cuts at: 0 40 381 477 773 964 1090 1250 1389
Size: 40 341 96 296 191 126 160 139
Cuts at: 1389 1689 1705 1741 1748 2158 2213 2222 2231
Size: 300 16 36 7 410 55 9 9
Cuts at: 2231 2675 2765 2819 2960 3041 3088 3230 3241
Size: 444 90 54 141 81 47 142 11
Cuts at: 3241 3286 3308 3370 3509 3516 3524 3862 4021
Size: 45 22 62 139 7 8 338 159
Cuts at: 4021 4194 4260 4269 4565 4947
Size: 173 66 9 296 382
Fragments arranged by size:
444 410 382 341 338 300 296 296
191 173 160 159 142 141 139 139
126 96 90 81 66 62 55 54
47 45 40 36 22 16 11 9
9 9 8 7 7
BspEI T'CCGG_A
Cuts at: 0 1063 2330 4380 4947
Size: 1063 1267 2050 567
Fragments arranged by size:
2050 1267 1063 567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 73 790 1520 1751 4226 4947
Size: 73 717 730 231 2475 721
Fragments arranged by size:
2475 730 721 717 231 73
BsrBI CCG'CTC
Cuts at: 0 1270 2871 2981 3173 3432 4947
Size: 1270 1601 110 192 259 1515
Fragments arranged by size:
1601 1515 1270 259 192 110
BsrDI GCAATG_nn'
Cuts at: 0 244 4947
Size: 244 4703
BsrFI r'CCGG_y
Cuts at: 0 660 2506 3152 4947
Size: 660 1846 646 1795
Fragments arranged by size:
1846 1795 660 646
BssSI C'ACGA_G
Cuts at: 0 3427 4947
Size: 3427 1520
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1917 2625 2728 4947
Size: 1917 708 103 2219
Fragments arranged by size:
2219 1917 708 103
BstEII G'GTnAC_C
Cuts at: 0 2877 4206 4758 4947
Size: 2877 1329 552 189
Fragments arranged by size:
2877 1329 552 189
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2819 3384 4194 4947
Size: 2819 565 810 753
Fragments arranged by size:
2819 810 753 565
BstYI r'GATC_y
Cuts at: 0 208 503 869 1099 1305 1320 1943 2114
Size: 208 295 366 230 206 15 623 171
Cuts at: 2114 4805 4947
Size: 2691 142
Fragments arranged by size:
2691 623 366 295 230 208 206 171
142 15
Bsu36I CC'TnA_GG
Cuts at: 0 4151 4947
Size: 4151 796
BtgI C'CryG_G
Cuts at: 0 356 875 1421 1439 1469 1784 2097 2265
Size: 356 519 546 18 30 315 313 168
Cuts at: 2265 3791 3944 4490 4947
Size: 1526 153 546 457
Fragments arranged by size:
1526 546 546 519 457 356 315 313
168 153 30 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 626 4947
Size: 626 4321
BtsI GCAGTG_nn'
Cuts at: 0 120 962 1341 1566 1687 1983 2729 4604
Size: 120 842 379 225 121 296 746 1875
Cuts at: 4604 4947
Size: 343
Fragments arranged by size:
1875 842 746 379 343 296 225 121
120
DrdI GACnn_nn'nnGTC
Cuts at: 0 403 3267 4947
Size: 403 2864 1680
Fragments arranged by size:
2864 1680 403
EaeI y'GGCC_r
Cuts at: 0 878 1424 3433 3550 3701 3941 3947 4727
Size: 878 546 2009 117 151 240 6 780
Cuts at: 4727 4847 4947
Size: 120 100
Fragments arranged by size:
2009 878 780 546 240 151 120 117
100 6
EagI C'GGCC_G
Cuts at: 0 3701 4947
Size: 3701 1246
EarI CTCTTCn'nnn_
Cuts at: 0 1006 1123 1128 1334 2452 3016 3300 3894
Size: 1006 117 5 206 1118 564 284 594
Cuts at: 3894 4744 4947
Size: 850 203
Fragments arranged by size:
1118 1006 850 594 564 284 206 203
117 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2688 4947
Size: 2688 2259
EcoICRI GAG'CTC
Cuts at: 0 475 1088 2958 3086 3860 4947
Size: 475 613 1870 128 774 1087
Fragments arranged by size:
1870 1087 774 613 475 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 271 634 1595 1854 2020 2358 2502
Size: 248 23 363 961 259 166 338 144
Cuts at: 2502 3147 3159 3406 3757 3934 4492 4947
Size: 645 12 247 351 177 558 455
Fragments arranged by size:
961 645 558 455 363 351 338 259
248 247 177 166 144 23 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 797 3619 4012 4789 4858 4947
Size: 797 2822 393 777 69 89
Fragments arranged by size:
2822 797 777 393 89 69
EcoO109I rG'GnC_Cy
Cuts at: 0 543 724 806 1763 1990 2738 2778 2963
Size: 543 181 82 957 227 748 40 185
Cuts at: 2963 3237 3238 3485 3711 4947
Size: 274 1 247 226 1236
Fragments arranged by size:
1236 957 748 543 274 247 227 226
185 181 82 40 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1932 4947
Size: 1932 3015
FspI TGC'GCA
Cuts at: 0 1198 4473 4789 4891 4947
Size: 1198 3275 316 102 56
Fragments arranged by size:
3275 1198 316 102 56
HaeII r_GCGC'y
Cuts at: 0 909 981 1461 1598 1795 2011 2525 3123
Size: 909 72 480 137 197 216 514 598
Cuts at: 3123 3613 4093 4241 4947
Size: 490 480 148 706
Fragments arranged by size:
909 706 598 514 490 480 480 216
197 148 137 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 2824 2856 3070 3102 3805 3837
Size: 166 32 2626 32 214 32 703 32
Cuts at: 3837 3844 3876 4099 4131 4947
Size: 7 32 223 32 816
Fragments arranged by size:
2626 816 703 223 214 166 32 32
32 32 32 32 7
HincII GTy'rAC
Cuts at: 0 2315 4947
Size: 2315 2632
KasI G'GCGC_C
Cuts at: 0 905 1457 1791 4089 4947
Size: 905 552 334 2298 858
Fragments arranged by size:
2298 905 858 552 334
KpnI G_GTAC'C
Cuts at: 0 624 4947
Size: 624 4323
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2522 2537 4208 4947
Size: 2522 15 1671 739
Fragments arranged by size:
2522 1671 739 15
MscI TGG'CCA
Cuts at: 0 1426 4729 4849 4947
Size: 1426 3303 120 98
Fragments arranged by size:
3303 1426 120 98
MslI CAynn'nnrTG
Cuts at: 0 152 967 4947
Size: 152 815 3980
Fragments arranged by size:
3980 815 152
MspA1I CmG'CkG
Cuts at: 0 286 715 1217 1471 1751 1786 2472 3135
Size: 286 429 502 254 280 35 686 663
Cuts at: 3135 3414 3748 4567 4947
Size: 279 334 819 380
Fragments arranged by size:
819 686 663 502 429 380 334 286
280 279 254 35
NaeI GCC'GGC
Cuts at: 0 3154 4947
Size: 3154 1793
NarI GG'CG_CC
Cuts at: 0 906 1458 1792 4090 4947
Size: 906 552 334 2298 857
Fragments arranged by size:
2298 906 857 552 334
NcoI C'CATG_G
Cuts at: 0 2265 4947
Size: 2265 2682
NgoMIV G'CCGG_C
Cuts at: 0 3152 4947
Size: 3152 1795
NheI G'CTAG_C
Cuts at: 0 4647 4947
Size: 4647 300
NspI r_CATG'y
Cuts at: 0 3682 4698 4890 4947
Size: 3682 1016 192 57
Fragments arranged by size:
3682 1016 192 57
PasI CC'CwG_GG
Cuts at: 0 40 1822 2819 3242 4947
Size: 40 1782 997 423 1705
Fragments arranged by size:
1782 1705 997 423 40
PciI A'CATG_T
Cuts at: 0 3678 4947
Size: 3678 1269
PflMI CCAn_nnn'nTGG
Cuts at: 0 357 1422 2781 4947
Size: 357 1065 1359 2166
Fragments arranged by size:
2166 1359 1065 357
PfoI T'CCnGG_A
Cuts at: 0 650 1450 1696 2343 3734 4947
Size: 650 800 246 647 1391 1213
Fragments arranged by size:
1391 1213 800 650 647 246
PmlI CAC'GTG
Cuts at: 0 4374 4947
Size: 4374 573
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 856 888 2669 2701 4947
Size: 856 32 1781 32 2246
Fragments arranged by size:
2246 1781 856 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 543 724 806 1763 3485 4947
Size: 543 181 82 957 1722 1462
Fragments arranged by size:
1722 1462 957 543 181 82
PspOMI G'GGCC_C
Cuts at: 0 3237 4947
Size: 3237 1710
PspXI vC'TCGA_Gb
Cuts at: 0 794 3087 4947
Size: 794 2293 1860
Fragments arranged by size:
2293 1860 794
PstI C_TGCA'G
Cuts at: 0 1409 1679 2933 3400 3722 4223 4611 4947
Size: 1409 270 1254 467 322 501 388 336
Fragments arranged by size:
1409 1254 501 467 388 336 322 270
PvuII CAG'CTG
Cuts at: 0 1217 1751 2472 3748 4947
Size: 1217 534 721 1276 1199
Fragments arranged by size:
1276 1217 1199 721 534
RsrII CG'GwC_CG
Cuts at: 0 2689 4947
Size: 2689 2258
SacI G_AGCT'C
Cuts at: 0 477 1090 2960 3088 3862 4947
Size: 477 613 1870 128 774 1085
Fragments arranged by size:
1870 1085 774 613 477 128
SacII CC_GC'GG
Cuts at: 0 1472 1787 4947
Size: 1472 315 3160
Fragments arranged by size:
3160 1472 315
SalI G'TCGA_C
Cuts at: 0 2313 4947
Size: 2313 2634
SanDI GG'GwC_CC
Cuts at: 0 724 4947
Size: 724 4223
SapI GCTCTTCn'nnn_
Cuts at: 0 1334 3300 4947
Size: 1334 1966 1647
Fragments arranged by size:
1966 1647 1334
SbfI CC_TGCA'GG
Cuts at: 0 3722 4223 4947
Size: 3722 501 724
Fragments arranged by size:
3722 724 501
SexAI A'CCwGG_T
Cuts at: 0 3652 4853 4947
Size: 3652 1201 94
Fragments arranged by size:
3652 1201 94
SfcI C'TryA_G
Cuts at: 0 1405 1675 2929 3396 3718 4219 4607 4947
Size: 1405 270 1254 467 322 501 388 340
Fragments arranged by size:
1405 1254 501 467 388 340 322 270
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3245 3709 4947
Size: 3245 464 1238
Fragments arranged by size:
3245 1238 464
SfoI GGC'GCC
Cuts at: 0 907 1459 1793 4091 4947
Size: 907 552 334 2298 856
Fragments arranged by size:
2298 907 856 552 334
SmaI CCC'GGG
Cuts at: 0 811 2215 2223 4264 4389 4947
Size: 811 1404 8 2041 125 558
Fragments arranged by size:
2041 1404 811 558 125 8
SmlI C'TyrA_G
Cuts at: 0 794 1947 3087 3822 4455 4947
Size: 794 1153 1140 735 633 492
Fragments arranged by size:
1153 1140 794 735 633 492
SspI AAT'ATT
Cuts at: 0 2340 4947
Size: 2340 2607
StuI AGG'CCT
Cuts at: 0 1203 4947
Size: 1203 3744
StyI C'CwwG_G
Cuts at: 0 1710 2265 3140 3209 3665 4763 4947
Size: 1710 555 875 69 456 1098 184
Fragments arranged by size:
1710 1098 875 555 456 184 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 71 2928 2956 3100 3524 4036 4086 4947
Size: 71 2857 28 144 424 512 50 861
Fragments arranged by size:
2857 861 512 424 144 71 50 28
TatI w'GTAC_w
Cuts at: 0 4676 4772 4877 4947
Size: 4676 96 105 70
Fragments arranged by size:
4676 105 96 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 564 596 3435 3467 4947
Size: 564 32 2839 32 1480
Fragments arranged by size:
2839 1480 564 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3561 4947
Size: 3561 1386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 942 3381 3942 4819 4947
Size: 942 2439 561 877 128
Fragments arranged by size:
2439 942 877 561 128
XhoI C'TCGA_G
Cuts at: 0 794 3087 4947
Size: 794 2293 1860
Fragments arranged by size:
2293 1860 794
XmaI C'CCGG_G
Cuts at: 0 809 2213 2221 4262 4387 4947
Size: 809 1404 8 2041 125 560
Fragments arranged by size:
2041 1404 809 560 125 8
ZraI GAC'GTC
Cuts at: 0 3263 4947
Size: 3263 1684
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII
Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfcI SfiI SfoI SmaI SmlI SspI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AhdI ApoI AscI AseI
AsiSI AvrII BaeI BclI BfrBI BsaBI BspHI BsrGI
BssHII BstBI BstZ17I ClaI CspCI DraI DraIII EcoRI
EcoRV FseI FspAI HindIII HpaI MfeI MluI NdeI
NotI NruI NsiI PacI PmeI PshAI PsiI PsrI
PvuI ScaI SgfI SgrAI SnaBI SpeI SphI SrfI
SwaI XbaI XmnI