(Circular) (Six-base) MAPSORT of: pFN21ASDA1538.seq Check: 3704 from: 1 to: 7768 pFN21ASDA1538 7768 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3412 4505 3412 Size: 1093 6675 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2118 5578 278 Size: 53 83 186 936 582 3460 2468 Fragments arranged by size: 3460 2468 936 582 186 83 53 AccI GT'mk_AC Cuts at: 1937 2816 4626 5111 1937 Size: 879 1810 485 4594 Fragments arranged by size: 4594 1810 879 485 Acc65I G'GTAC_C Cuts at: 2578 3114 5089 2578 Size: 536 1975 5257 Fragments arranged by size: 5257 1975 536 AclI AA'CG_TT Cuts at: 1563 5895 6268 1563 Size: 4332 373 3063 Fragments arranged by size: 4332 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2170 2190 2531 2637 3014 3182 4819 5831 Size: 538 20 341 106 377 168 1637 1012 Cuts at: 5831 7215 7483 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1637 1384 1012 538 377 341 268 168 106 20 AfeI AGC'GCT Cuts at: 4441 4441 Size: 7768 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7571 AflIII A'CryG_T Cuts at: 1203 6667 7644 1203 Size: 5464 977 1327 Fragments arranged by size: 5464 1327 977 AgeI A'CCGG_T Cuts at: 6599 6599 Size: 7768 AhdI GACnn_n'nnGTC Cuts at: 6497 6497 Size: 7768 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7768 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4103 4398 1184 Size: 2919 295 4554 Fragments arranged by size: 4554 2919 295 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2079 2111 2079 Size: 32 7736 AlwNI CAG_nnn'CTG Cuts at: 2234 2423 2554 3416 3785 3884 4126 5511 7083 Size: 189 131 862 369 99 242 1385 1572 Cuts at: 7083 2234 Size: 2919 Fragments arranged by size: 2919 1572 1385 862 369 242 189 131 99 ApaI G_GGCC'C Cuts at: 2637 2643 2909 3797 4903 2637 Size: 6 266 888 1106 5502 Fragments arranged by size: 5502 1106 888 266 6 ApaLI G'TGCA_C Cuts at: 5825 6981 5825 Size: 1156 6612 ApoI r'AATT_y Cuts at: 1493 4643 5077 5303 5455 7564 1493 Size: 3150 434 226 152 2109 1697 Fragments arranged by size: 3150 2109 1697 434 226 152 AseI AT'TA_AT Cuts at: 160 1032 6322 160 Size: 872 5290 1606 Fragments arranged by size: 5290 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7768 AvaI C'yCGr_G Cuts at: 1870 1943 2391 2636 2909 3196 3562 4092 4287 Size: 73 448 245 273 287 366 530 195 Cuts at: 4287 4397 4454 5093 1870 Size: 110 57 639 4545 Fragments arranged by size: 4545 639 530 448 366 287 273 245 195 110 73 57 BamHI G'GATC_C Cuts at: 5098 5465 5098 Size: 367 7401 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2240 2523 2578 3114 4064 Size: 325 217 627 453 283 55 536 950 Cuts at: 4064 4197 4403 4445 5089 6544 618 Size: 133 206 42 644 1455 1842 Fragments arranged by size: 1842 1455 950 644 627 536 453 325 283 217 206 133 55 42 BanII G_rGCy'C Cuts at: 1391 2637 2643 2909 3325 3797 4169 4277 4596 Size: 1246 6 266 416 472 372 108 319 Cuts at: 4596 4785 4903 5087 1391 Size: 189 118 184 4072 Fragments arranged by size: 4072 1246 472 416 372 319 266 189 184 118 108 6 BbeI G_GCGC'C Cuts at: 2244 2527 2244 Size: 283 7485 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2292 3170 3224 928 Size: 971 393 878 54 5472 Fragments arranged by size: 5472 971 878 393 54 BbvCI CC'TCA_GC Cuts at: 2676 2872 4536 2676 Size: 196 1664 5908 Fragments arranged by size: 5908 1664 196 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5959 5993 1903 Size: 34 4022 34 3678 Fragments arranged by size: 4022 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5660 6870 5660 Size: 1210 6558 BclI T'GATC_A Cuts at: 1548 1548 Size: 7768 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2532 2550 2600 Size: 107 122 71 631 605 860 18 50 Cuts at: 2600 2716 3329 3368 3394 6379 136 Size: 116 613 39 26 2985 1525 Fragments arranged by size: 2985 1525 860 631 613 605 122 116 107 71 50 39 26 18 BglII A'GATC_T Cuts at: 7763 7763 Size: 7768 BlpI GC'TnA_GC Cuts at: 3905 4389 5185 3905 Size: 484 796 6488 Fragments arranged by size: 6488 796 484 Bme1580I G_kGCm'C Cuts at: 1790 2637 2643 2909 3797 4183 4219 4406 4903 Size: 847 6 266 888 386 36 187 497 Cuts at: 4903 5829 6985 1790 Size: 926 1156 2573 Fragments arranged by size: 2573 1156 926 888 847 497 386 266 187 36 6 BmgBI CAC'GTC Cuts at: 1318 4178 1318 Size: 2860 4908 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2244 2728 2779 Size: 416 53 83 399 24 827 484 51 Cuts at: 2779 4592 5499 6457 442 Size: 1813 907 958 1753 Fragments arranged by size: 1813 1753 958 907 827 484 416 399 83 53 51 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7768 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4573 4573 Size: 7768 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2346 2420 4033 4071 4080 4547 6428 1239 Size: 1107 74 1613 38 9 467 1881 2579 Fragments arranged by size: 2579 1881 1613 1107 467 74 38 9 Bpu10I CC'TnA_GC Cuts at: 2676 2872 3603 4038 4361 4536 4779 2676 Size: 196 731 435 323 175 243 5665 Fragments arranged by size: 5665 731 435 323 243 196 175 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3242 4929 5893 6758 7056 3242 Size: 1687 964 865 298 3954 Fragments arranged by size: 3954 1687 964 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3131 4544 6431 882 Size: 617 1632 1413 1887 2219 Fragments arranged by size: 2219 1887 1632 1413 617 BsaAI yAC'GTr Cuts at: 493 1204 2917 2941 2977 5065 5559 493 Size: 711 1713 24 36 2088 494 2702 Fragments arranged by size: 2702 2088 1713 711 494 36 24 BsaBI GATnn'nnATC Cuts at: 7762 7762 Size: 7768 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2115 2241 2524 2868 Size: 53 83 186 936 582 126 283 344 Cuts at: 2868 5575 5957 6592 275 Size: 2707 382 635 1451 Fragments arranged by size: 2707 1451 936 635 582 382 344 283 186 126 83 53 BsaWI w'CCGG_w Cuts at: 4737 6201 6599 6873 7020 7431 4737 Size: 1464 398 274 147 411 5074 Fragments arranged by size: 5074 1464 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2079 2109 3098 3128 4960 Size: 30 165 30 676 30 989 30 1832 Cuts at: 4960 4975 4990 5005 1178 Size: 15 15 15 3941 Fragments arranged by size: 3941 1832 989 676 165 30 30 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2272 2275 2278 2308 3585 3701 3866 3929 Size: 1086 3 3 30 1277 116 165 63 Cuts at: 3929 3938 3947 3959 3962 3974 3977 3980 3983 Size: 9 9 12 3 12 3 3 3 Cuts at: 3983 3992 4150 4486 4604 1186 Size: 9 158 336 118 4350 Fragments arranged by size: 4350 1277 1086 336 165 158 118 116 63 30 12 12 9 9 9 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2416 2590 2651 2824 2831 3451 3628 3646 4140 Size: 174 61 173 7 620 177 18 494 Cuts at: 4140 4153 4168 4806 6971 7467 2416 Size: 13 15 638 2165 496 2717 Fragments arranged by size: 2717 2165 638 620 496 494 177 174 173 61 18 15 13 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4658 1163 Size: 573 2922 4273 Fragments arranged by size: 4273 2922 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4161 4672 5218 5979 7007 1540 Size: 275 186 2160 511 546 761 1028 2301 Fragments arranged by size: 2301 2160 1028 761 546 511 275 186 BsiHKAI G_wGCw'C Cuts at: 2705 2916 4529 5829 5914 6985 2705 Size: 211 1613 1300 85 1071 3488 Fragments arranged by size: 3488 1613 1300 1071 211 85 BsmI GAATG_Cn' Cuts at: 5279 5372 5279 Size: 93 7675 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2637 2643 2705 2909 2916 3325 3797 Size: 399 847 6 62 204 7 409 472 Cuts at: 3797 4169 4183 4219 4277 4406 4529 4596 4785 Size: 372 14 36 58 129 123 67 189 Cuts at: 4785 4903 5087 5829 5914 6985 1391 Size: 118 184 742 85 1071 2174 Fragments arranged by size: 2174 1071 847 742 472 409 399 372 204 189 184 129 123 118 85 67 62 58 36 14 7 6 BspEI T'CCGG_A Cuts at: 7431 7431 Size: 7768 BspHI T'CATG_A Cuts at: 5657 7387 5657 Size: 1730 6038 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3102 3412 3897 4328 4505 5123 844 Size: 2258 310 485 431 177 618 3489 Fragments arranged by size: 3489 2258 618 485 431 310 177 BsrBI CCG'CTC Cuts at: 1394 1823 3909 4192 4393 4792 4850 5655 1394 Size: 429 2086 283 201 399 58 805 3507 Fragments arranged by size: 3507 2086 805 429 399 283 201 58 BsrDI GCAATG_nn' Cuts at: 65 1248 3013 3378 4419 6263 6437 65 Size: 1183 1765 365 1041 1844 174 1396 Fragments arranged by size: 1844 1765 1396 1183 1041 365 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3358 4101 6412 6599 1700 Size: 110 1548 743 2311 187 2869 Fragments arranged by size: 2869 2311 1548 743 187 110 BsrGI T'GTAC_A Cuts at: 96 3010 96 Size: 2914 4854 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7768 BssSI C'ACGA_G Cuts at: 3016 5828 6840 3016 Size: 2812 1012 3944 Fragments arranged by size: 3944 2812 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4958 794 Size: 4164 3604 BstBI TT'CG_AA Cuts at: 2007 6572 2007 Size: 4565 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7768 BstXI CCAn_nnnn'nTGG Cuts at: 3745 4113 3745 Size: 368 7400 BstYI r'GATC_y Cuts at: 1972 5098 5465 5850 5867 7319 7405 7417 7763 Size: 3126 367 385 17 1452 86 12 346 Cuts at: 7763 1972 Size: 1977 Fragments arranged by size: 3126 1977 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 4627 4627 Size: 7768 Bsu36I CC'TnA_GG Cuts at: 5007 5007 Size: 7768 BtgI C'CryG_G Cuts at: 513 1065 2098 2598 3184 3472 3669 3681 3707 Size: 552 1033 500 586 288 197 12 26 Cuts at: 3707 4264 4369 513 Size: 557 105 3912 Fragments arranged by size: 3912 1033 586 557 552 500 288 197 105 26 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5067 487 Size: 683 56 3841 3188 Fragments arranged by size: 3841 3188 683 56 BtsI GCAGTG_nn' Cuts at: 2468 2705 3493 3552 4087 4327 4527 4941 5284 Size: 237 788 59 535 240 200 414 343 Cuts at: 5284 6078 6098 2468 Size: 794 20 4138 Fragments arranged by size: 4138 794 788 535 414 343 240 237 200 59 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7768 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4638 4673 529 Size: 35 4074 35 3624 Fragments arranged by size: 4074 3624 35 35 DraI TTT'AAA Cuts at: 5072 5072 Size: 7768 DraIII CAC_nnn'GTG Cuts at: 2920 2920 Size: 7768 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2050 6775 809 Size: 507 734 4725 1802 Fragments arranged by size: 4725 1802 734 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2601 2935 3510 Size: 54 1425 50 275 18 771 334 575 Cuts at: 3510 3672 3787 4103 4158 4669 4845 5215 6104 Size: 162 115 316 55 511 176 370 889 Cuts at: 6104 7733 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 771 575 511 370 334 316 275 176 162 115 55 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4158 4669 5215 1537 Size: 275 2346 511 546 4090 Fragments arranged by size: 4090 2346 546 511 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2111 2281 2296 2314 2347 2353 2613 2990 Size: 758 170 15 18 33 6 260 377 Cuts at: 2990 3936 3951 4992 5698 1353 Size: 946 15 1041 706 3423 Fragments arranged by size: 3423 1041 946 758 706 377 260 170 33 18 15 15 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3037 3083 6355 6729 6875 387 Size: 1064 138 1448 46 3272 374 146 1280 Fragments arranged by size: 3272 1448 1280 1064 374 146 138 46 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2170 2190 2346 2420 2531 2637 3014 Size: 393 538 20 156 74 111 106 377 Cuts at: 3014 3182 4033 4071 4080 4547 4819 5831 6428 Size: 168 851 38 9 467 272 1012 597 Cuts at: 6428 7215 7483 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1012 851 787 597 538 467 393 377 272 268 168 156 111 106 74 38 20 9 EcoNI CCTnn'n_nnAGG Cuts at: 2558 2558 Size: 7768 EcoO109I rG'GnC_Cy Cuts at: 2194 2395 2551 2686 2905 2906 3525 3770 3793 Size: 201 156 135 219 1 619 245 23 Cuts at: 3793 3794 4017 4302 4567 4899 4900 2194 Size: 1 223 285 265 332 1 5062 Fragments arranged by size: 5062 619 332 285 265 245 223 219 201 156 135 23 1 1 1 EcoRI G'AATT_C Cuts at: 5077 5455 5077 Size: 378 7390 FspI TGC'GCA Cuts at: 6274 6274 Size: 7768 HaeII r_GCGC'y Cuts at: 1678 2244 2460 2527 2841 3871 4443 6915 1678 Size: 566 216 67 314 1030 572 2472 2531 Fragments arranged by size: 2531 2472 1030 572 566 314 216 67 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3968 4000 6409 6441 6483 Size: 32 135 32 2396 32 2409 32 42 Cuts at: 6483 6515 1373 Size: 32 2626 Fragments arranged by size: 2626 2409 2396 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5112 5358 669 Size: 988 281 3174 246 3079 Fragments arranged by size: 3174 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2746 4542 748 Size: 1998 1796 3974 Fragments arranged by size: 3974 1998 1796 HpaI GTT'AAC Cuts at: 5358 5358 Size: 7768 KasI G'GCGC_C Cuts at: 2240 2523 2240 Size: 283 7485 KpnI G_GTAC'C Cuts at: 2582 3118 5093 2582 Size: 536 1975 5257 Fragments arranged by size: 5257 1975 536 MfeI C'AATT_G Cuts at: 5367 5367 Size: 7768 MluI A'CGCG_T Cuts at: 7644 7644 Size: 7768 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3368 4094 6882 7066 1305 Size: 2063 726 2788 184 2007 Fragments arranged by size: 2788 2063 2007 726 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2603 3674 10 Size: 54 1425 343 771 1071 4104 Fragments arranged by size: 4104 1425 1071 771 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3006 5726 6085 6244 518 Size: 648 1840 2720 359 159 2042 Fragments arranged by size: 2720 2042 1840 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2092 2270 2423 2498 2651 3656 3805 4157 Size: 486 178 153 75 153 1005 149 352 Cuts at: 4157 4172 4355 4506 5184 5861 7009 7254 1606 Size: 15 183 151 678 677 1148 245 2120 Fragments arranged by size: 2120 1148 1005 678 677 486 352 245 183 178 153 153 151 149 75 15 NaeI GCC'GGC Cuts at: 4103 4103 Size: 7768 NarI GG'CG_CC Cuts at: 2241 2524 2241 Size: 283 7485 NcoI C'CATG_G Cuts at: 513 1065 2598 3472 3669 513 Size: 552 1533 874 197 4612 Fragments arranged by size: 4612 1533 874 552 197 NdeI CA'TA_TG Cuts at: 387 387 Size: 7768 NgoMIV G'CCGG_C Cuts at: 4101 4101 Size: 7768 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7768 NotI GC'GGCC_GC Cuts at: 5215 5215 Size: 7768 NspI r_CATG'y Cuts at: 1132 1229 4385 5126 6671 1132 Size: 97 3156 741 1545 2229 Fragments arranged by size: 3156 2229 1545 741 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7768 PciI A'CATG_T Cuts at: 6667 6667 Size: 7768 PflMI CCAn_nnn'nTGG Cuts at: 1108 2234 3416 3713 1108 Size: 1126 1182 297 5163 Fragments arranged by size: 5163 1182 1126 297 PfoI T'CCnGG_A Cuts at: 2397 4563 2397 Size: 2166 5602 PmeI GTTT'AAAC Cuts at: 5072 5072 Size: 7768 PmlI CAC'GTG Cuts at: 2917 2941 2917 Size: 24 7744 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2079 2111 5837 5869 7350 7382 2079 Size: 32 3726 32 1481 32 2465 Fragments arranged by size: 3726 2465 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2395 3525 3770 4567 2395 Size: 1130 245 797 5596 Fragments arranged by size: 5596 1130 797 245 PshAI GACnn'nnGTC Cuts at: 1613 3143 3891 1613 Size: 1530 748 5490 Fragments arranged by size: 5490 1530 748 PsiI TTA'TAA Cuts at: 5338 5338 Size: 7768 PspOMI G'GGCC_C Cuts at: 2633 2639 2905 3793 4899 2633 Size: 6 266 888 1106 5502 Fragments arranged by size: 5502 1106 888 266 6 PstI C_TGCA'G Cuts at: 830 2038 2772 2860 3613 3661 3810 5120 830 Size: 1208 734 88 753 48 149 1310 3478 Fragments arranged by size: 3478 1310 1208 753 734 149 88 48 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7768 PvuII CAG'CTG Cuts at: 2270 2423 2498 2651 3805 4355 2270 Size: 153 75 153 1154 550 5683 Fragments arranged by size: 5683 1154 550 153 153 75 SalI G'TCGA_C Cuts at: 1936 5110 1936 Size: 3174 4594 SanDI GG'GwC_CC Cuts at: 3525 3770 3525 Size: 245 7523 SbfI CC_TGCA'GG Cuts at: 2860 5120 2860 Size: 2260 5508 ScaI AGT'ACT Cuts at: 6016 7728 6016 Size: 1712 6056 SfcI C'TryA_G Cuts at: 826 1045 2034 2452 2499 2768 2817 2856 3310 Size: 219 989 418 47 269 49 39 454 Cuts at: 3310 3609 3657 3806 5116 6251 6932 7123 826 Size: 299 48 149 1310 1135 681 191 1471 Fragments arranged by size: 1471 1310 1135 989 681 454 418 299 269 219 191 149 49 48 47 39 SfoI GGC'GCC Cuts at: 2242 2525 2242 Size: 283 7485 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7768 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7768 SmaI CCC'GGG Cuts at: 1872 2393 2638 3198 4289 4399 4456 5095 1872 Size: 521 245 560 1091 110 57 639 4545 Fragments arranged by size: 4545 1091 639 560 521 245 110 57 SmlI C'TyrA_G Cuts at: 820 1017 1943 3257 4944 5872 6773 7035 820 Size: 197 926 1314 1687 928 901 262 1553 Fragments arranged by size: 1687 1553 1314 928 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5065 5559 493 Size: 4572 494 2702 Fragments arranged by size: 4572 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7768 SphI G_CATG'C Cuts at: 1132 4385 5126 1132 Size: 3253 741 3774 Fragments arranged by size: 3774 3253 741 SrfI GCCC'GGGC Cuts at: 2638 3198 2638 Size: 560 7208 SspI AAT'ATT Cuts at: 5 52 5692 5 Size: 47 5640 2081 Fragments arranged by size: 5640 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7768 StyI C'CwwG_G Cuts at: 513 1065 2598 3472 3669 3693 4335 4431 5207 Size: 552 1533 874 197 24 642 96 776 Cuts at: 5207 513 Size: 3074 Fragments arranged by size: 3074 1533 874 776 642 552 197 96 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2263 3238 3465 3586 3634 5795 5812 Size: 1255 683 975 227 121 48 2161 17 Cuts at: 5812 5965 6150 6595 325 Size: 153 185 445 1498 Fragments arranged by size: 2161 1498 1255 975 683 445 227 185 153 121 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3010 6014 7726 Size: 274 80 33 51 1443 1033 3004 1712 Cuts at: 7726 96 Size: 138 Fragments arranged by size: 3004 1712 1443 1033 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2379 2411 7728 7760 2379 Size: 32 5317 32 2387 Fragments arranged by size: 5317 2387 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2983 1316 Size: 1667 6101 XbaI T'CTAG_A Cuts at: 5104 5104 Size: 7768 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2655 2655 Size: 7768 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7768 XmaI C'CCGG_G Cuts at: 1870 2391 2636 3196 4287 4397 4454 5093 1870 Size: 521 245 560 1091 110 57 639 4545 Fragments arranged by size: 4545 1091 639 560 521 245 110 57 XmnI GAAnn'nnTTC Cuts at: 5897 5897 Size: 7768 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2116 5576 276 Size: 53 83 186 936 582 3460 2468 Fragments arranged by size: 3460 2468 936 582 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BsmBI EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SwaI