Restriction map for
pFN21ASDA1519
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BbvCI BclI BfaI BfrI
BglII BlnI BsaBI BsaHI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RcaI
RsrII SacII SalI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AflIII 881 ACACGT A^C[GA][CT]GT
AocI 1150 CCTGAGG CC^T[ACGT]AGG
Asp700I 277 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1137 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 548 TGGCCA TGG^CCA
BanI 963 GGTGCC G^G[CT][GA]CC
BcgI 1173 GCAGAGGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 912 GGATAC GTATCC(6/5)
BsaAI 882 CACGTA [CT]AC^GT[GA]
BsaI 1178 GGTCTC GGTCTC(1/5)
BsiEI 405 CGATCG CG[GA][CT]^CG
BsrDI 600 CATTGC GCAATG(2/0)
BstXI 813 CCAGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1150 CCTGAGG CC^T[ACGT]AGG
DrdI 580 GACGAGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 548 TGGCCA [CT]^GGCC[GA]
Eco81I 1150 CCTGAGG CC^T[ACGT]AGG
EcoRI 1375 GAATTC G^AATTC
MluNI 548 TGGCCA TGG^CCA
MscI 548 TGGCCA TGG^CCA
MslI 1752 CACGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI 1137 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 393 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 405 CGATCG CGAT^CG
SapI 51 GAAGAGC GCTCTTC(1/4)
SbfI 1251 CCTGCAGG CCTGCA^GG
SexAI 1283 ACCTGGT A^CC[AT]GGT
SmlI 1879 CTCAAG C^T[CT][GA]AG
Sse8387I 1251 CCTGCAGG CCTGCA^GG
TaqI 470 TCGA T^CGA
Tth111I 1137 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 470 TCGA T^CGA
Van91I 393 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 551 CCAAGCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 277 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 1375 GAATTC [GA]^AATT[CT]
1650 AAATTT
Alw44I 373 GTGCAC G^TGCAC
534 GTGCAC
AlwI 1740 GATCC GGATC(4/5)
177 GGATC
ApaLI 373 GTGCAC G^TGCAC
534 GTGCAC
ApoI 1375 GAATTC [GA]^AATT[CT]
1650 AAATTT
BbsI 1242 GAAGAC GAAGAC(2/6)
1884 GAAGAC
BglI 1249 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1919 GCCATGGAGGC
BpuAI 1242 GAAGAC GAAGAC(2/6)
1884 GAAGAC
BsrI 1862 ACTGG ACTGG(1/-1)
729 CCAGT
BstYI 177 GGATCT [GA]^GATC[CT]
1739 AGATCC
EcoNI 1338 CCTTGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1803 CCTGCTGCAGG
HaeII 1260 GGCGCT [GA]GCGC^[CT]
1912 AGCGCC
HgaI 1272 GACGC GACGC(5/10)
485 GCGTC
MflI 177 GGATCT [GA]^GATC[CT]
1739 AGATCC
MspA1I 397 CAGCTG C[AC]G^C[GT]G
774 CAGCTG
PvuII 397 CAGCTG CAG^CTG
774 CAGCTG
TfiI 691 GAATC G^A[AT]TC
1022 GATTC
Tsp509I 1376 AATT ^AATT
1651 AATT
XhoII 177 GGATCT [GA]^GATC[CT]
1739 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI AvaII BanII BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BssKI
BssSI BstAPI BstF5I BstNI BstUI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MseI
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StuI StyI Tru9I TseI Tsp45I
TspRI XmaCI XmaI