Restriction map for
pFN21ASDA1519
(Circular) (Six-base) MAPSORT of: pFN21ASDA1519.seq Check: 8607 from: 1 to: 6670
pFN21ASDA1519 6670 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2409 3834 2409
Size: 1425 5245
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4480 278
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4013 1937
Size: 2076 4594
Acc65I G'GTAC_C
Cuts at: 3991 3991
Size: 6670
AclI AA'CG_TT
Cuts at: 1563 4797 5170 1563
Size: 3234 373 3063
Fragments arranged by size:
3234 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2656 3755 4733 6117 6385 1632
Size: 464 560 1099 978 1384 268 1917
Fragments arranged by size:
1917 1384 1099 978 560 464 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6473
AflIII A'CryG_T
Cuts at: 1203 2904 5569 6546 1203
Size: 1701 2665 977 1327
Fragments arranged by size:
2665 1701 1327 977
AgeI A'CCGG_T
Cuts at: 5501 5501
Size: 6670
AhdI GACnn_n'nnGTC
Cuts at: 5399 5399
Size: 6670
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6670
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3356 1184
Size: 2172 4498
AlwNI CAG_nnn'CTG
Cuts at: 2245 2422 2584 3321 3659 3745 3784 3838 4413
Size: 177 162 737 338 86 39 54 575
Cuts at: 4413 5985 2245
Size: 1572 2930
Fragments arranged by size:
2930 1572 737 575 338 177 162 86
54 39
ApaLI G'TGCA_C
Cuts at: 2396 2557 4727 5883 2396
Size: 161 2170 1156 3183
Fragments arranged by size:
3183 2170 1156 161
ApoI r'AATT_y
Cuts at: 1493 3398 3673 3979 4205 4357 6466 1493
Size: 1905 275 306 226 152 2109 1697
Fragments arranged by size:
2109 1905 1697 306 275 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5224 160
Size: 872 4192 1606
Fragments arranged by size:
4192 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
AvaI C'yCGr_G
Cuts at: 1870 1943 2657 3204 3236 3257 3510 3995 1870
Size: 73 714 547 32 21 253 485 4545
Fragments arranged by size:
4545 714 547 485 253 73 32 21
BamHI G'GATC_C
Cuts at: 4000 4367 4000
Size: 367 6303
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2986 3991 5446 618
Size: 325 217 627 1199 1005 1455 1842
Fragments arranged by size:
1842 1455 1199 1005 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2331 2735 3774 3903 3989 1391
Size: 940 404 1039 129 86 4072
Fragments arranged by size:
4072 1039 940 404 129 86
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3272 3914 928
Size: 971 1373 642 3684
Fragments arranged by size:
3684 1373 971 642
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3185 3219 4861 4895 1903
Size: 34 1248 34 1642 34 3678
Fragments arranged by size:
3678 1642 1248 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2929 4562 5772 2929
Size: 1633 1210 3827
Fragments arranged by size:
3827 1633 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6670
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3278 3948 5281
Size: 107 122 71 631 605 1606 670 1333
Cuts at: 5281 136
Size: 1525
Fragments arranged by size:
1606 1525 1333 670 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6665 6665
Size: 6670
BlpI GC'TnA_GC
Cuts at: 2051 2106 3489 3839 4087 2051
Size: 55 1383 350 248 4634
Fragments arranged by size:
4634 1383 350 248 55
Bme1580I G_kGCm'C
Cuts at: 1790 2400 2561 2893 3111 4731 5887 1790
Size: 610 161 332 218 1620 1156 2573
Fragments arranged by size:
2573 1620 1156 610 332 218 161
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6670
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4401 5359 442
Size: 416 53 83 399 24 2984 958 1753
Fragments arranged by size:
2984 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6670
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 6670
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2399 2837 3311 3479 3767 3907 5330
Size: 1010 150 438 474 168 288 140 1423
Cuts at: 5330 1239
Size: 2579
Fragments arranged by size:
2579 1423 1010 474 438 288 168 150
140
Bpu10I CC'TnA_GC
Cuts at: 2083 3122 3366 3411 2083
Size: 1039 244 45 5342
Fragments arranged by size:
5342 1039 244 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3887 4795 5660 5958 3887
Size: 908 865 298 4599
Fragments arranged by size:
4599 908 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3207 5333 882
Size: 617 1708 2126 2219
Fragments arranged by size:
2219 2126 1708 617
BsaAI yAC'GTr
Cuts at: 493 1204 2907 3967 4461 493
Size: 711 1703 1060 494 2702
Fragments arranged by size:
2702 1703 1060 711 494
BsaBI GATnn'nnATC
Cuts at: 6664 6664
Size: 6670
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4477 4859 5494 275
Size: 53 83 186 936 2944 382 635 1451
Fragments arranged by size:
2944 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5103 5501 5775 5922 6333 5103
Size: 398 274 147 411 5440
Fragments arranged by size:
5440 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2270 2300 2912 2942 2970
Size: 30 165 30 867 30 612 30 28
Cuts at: 2970 3000 3185 3215 3452 3482 1178
Size: 30 185 30 237 30 4366
Fragments arranged by size:
4366 867 612 237 185 165 30 30
30 30 30 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2278 2803 2806 3206 1186
Size: 886 206 525 3 400 4650
Fragments arranged by size:
4650 886 525 400 206 3
BseYI C'CCAG_C
Cuts at: 2512 3321 5873 6369 2512
Size: 809 2552 496 2813
Fragments arranged by size:
2813 2552 809 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3037 3326 3524 3672 1163
Size: 573 1301 289 198 148 4161
Fragments arranged by size:
4161 1301 573 289 198 148
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2431 4120 4881 5909 1540
Size: 275 186 430 1689 761 1028 2301
Fragments arranged by size:
2301 1689 1028 761 430 275 186
BsiHKAI G_wGCw'C
Cuts at: 2331 2400 2561 2666 3753 3774 3903 4731 4816
Size: 69 161 105 1087 21 129 828 85
Cuts at: 4816 5887 2331
Size: 1071 3114
Fragments arranged by size:
3114 1087 1071 828 161 129 105 85
69 21
BsmI GAATG_Cn'
Cuts at: 2235 3036 3356 4181 4274 2235
Size: 801 320 825 93 4631
Fragments arranged by size:
4631 825 801 320 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2777 3237 3287 2777
Size: 460 50 6160
Fragments arranged by size:
6160 460 50
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2331 2400 2561 2666 2735 2893 3111
Size: 399 541 69 161 105 69 158 218
Cuts at: 3111 3753 3774 3903 3989 4731 4816 5887 1391
Size: 642 21 129 86 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 642 541 399 218 161
158 129 105 86 85 69 69 21
BspEI T'CCGG_A
Cuts at: 6333 6333
Size: 6670
BspHI T'CATG_A
Cuts at: 4559 6289 4559
Size: 1730 4940
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2409 3324 3834 4025 844
Size: 1565 915 510 191 3489
Fragments arranged by size:
3489 1565 915 510 191
BsrBI CCG'CTC
Cuts at: 1394 1823 2079 2452 2870 4557 1394
Size: 429 256 373 418 1687 3507
Fragments arranged by size:
3507 1687 429 418 373 256
BsrDI GCAATG_nn'
Cuts at: 65 1248 2622 5165 5339 65
Size: 1183 1374 2543 174 1396
Fragments arranged by size:
2543 1396 1374 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5314 5501 1700
Size: 110 3504 187 2869
Fragments arranged by size:
3504 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6670
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6670
BssSI C'ACGA_G
Cuts at: 2330 2665 2700 4730 5742 2330
Size: 335 35 2030 1012 3258
Fragments arranged by size:
3258 2030 1012 335 35
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2808 3362 3659 3784 794
Size: 2014 554 297 125 3680
Fragments arranged by size:
3680 2014 554 297 125
BstBI TT'CG_AA
Cuts at: 2007 5474 2007
Size: 3467 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6670
BstXI CCAn_nnnn'nTGG
Cuts at: 2843 2843
Size: 6670
BstYI r'GATC_y
Cuts at: 1972 2200 3762 4000 4367 4752 4769 6221 6307
Size: 228 1562 238 367 385 17 1452 86
Cuts at: 6307 6319 6665 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1562 1452 385 367 346 238 228
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3174 3174
Size: 6670
BtgI C'CryG_G
Cuts at: 513 1065 2022 2358 2931 3912 3943 513
Size: 552 957 336 573 981 31 3240
Fragments arranged by size:
3240 981 957 573 552 336 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3440 487
Size: 683 56 2214 3717
Fragments arranged by size:
3717 2214 683 56
BtsI GCAGTG_nn'
Cuts at: 3657 4186 4980 5000 3657
Size: 529 794 20 5327
Fragments arranged by size:
5327 794 529 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6670
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6635
DraI TTT'AAA
Cuts at: 3974 3974
Size: 6670
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2609 5677 809
Size: 507 1293 3068 1802
Fragments arranged by size:
3068 1802 1293 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2571 4117 5006
Size: 54 1425 50 275 18 741 1546 889
Cuts at: 5006 6635 8
Size: 1629 43
Fragments arranged by size:
1629 1546 1425 889 741 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4117 1537
Size: 275 2305 4090
Fragments arranged by size:
4090 2305 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2069 2959 3591 3641 3701 4600 1353
Size: 716 890 632 50 60 899 3423
Fragments arranged by size:
3423 899 890 716 632 60 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2844 3548 5257 5631 5777 387
Size: 1064 138 1255 704 1709 374 146 1280
Fragments arranged by size:
1709 1280 1255 1064 704 374 146 138
EcoICRI GAG'CTC
Cuts at: 2329 3772 3901 2329
Size: 1443 129 5098
Fragments arranged by size:
5098 1443 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2249 2399 2656 2837 3311 3479
Size: 393 464 153 150 257 181 474 168
Cuts at: 3479 3755 3767 3907 4733 5330 6117 6385 1239
Size: 276 12 140 826 597 787 268 1524
Fragments arranged by size:
1524 826 787 597 474 464 393 276
268 257 181 168 153 150 140 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3365 3830 3365
Size: 465 6205
EcoO109I rG'GnC_Cy
Cuts at: 2087 2641 3099 3159 3336 2087
Size: 554 458 60 177 5421
Fragments arranged by size:
5421 554 458 177 60
EcoRI G'AATT_C
Cuts at: 3398 3979 4357 3398
Size: 581 378 5711
Fragments arranged by size:
5711 581 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2098 2098
Size: 6670
FspI TGC'GCA
Cuts at: 5176 5176
Size: 6670
HaeII r_GCGC'y
Cuts at: 1678 3287 3939 5817 1678
Size: 1609 652 1878 2531
Fragments arranged by size:
2531 1878 1609 652
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2099 2131 2251 2283 2936
Size: 32 135 32 527 32 120 32 653
Cuts at: 2936 2968 3151 3183 3185 3217 3431 3452 3463
Size: 32 183 32 2 32 214 21 11
Cuts at: 3463 3484 5311 5343 5385 5417 1373
Size: 21 1827 32 42 32 2626
Fragments arranged by size:
2626 1827 653 527 214 183 135 120
42 32 32 32 32 32 32 32
32 32 21 21 11 2
HincII GTy'rAC
Cuts at: 669 1657 1938 4014 4260 669
Size: 988 281 2076 246 3079
Fragments arranged by size:
3079 2076 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6670
HpaI GTT'AAC
Cuts at: 4260 4260
Size: 6670
KpnI G_GTAC'C
Cuts at: 3995 3995
Size: 6670
MfeI C'AATT_G
Cuts at: 4269 4269
Size: 6670
MluI A'CGCG_T
Cuts at: 6546 6546
Size: 6670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2946 3019 5784 5968 1305
Size: 1641 73 2765 184 2007
Fragments arranged by size:
2765 2007 1641 184 73
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2573 10
Size: 54 1425 343 741 4107
Fragments arranged by size:
4107 1425 741 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3779 4628 4987 5146 518
Size: 648 2613 849 359 159 2042
Fragments arranged by size:
2613 2042 849 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2422 2799 4086 4763 5911 6156 1606
Size: 816 377 1287 677 1148 245 2120
Fragments arranged by size:
2120 1287 1148 816 677 377 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2931 3912 3943 513
Size: 552 957 909 981 31 3240
Fragments arranged by size:
3240 981 957 909 552 31
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6670
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6670
NotI GC'GGCC_GC
Cuts at: 4117 4117
Size: 6670
NspI r_CATG'y
Cuts at: 1132 1229 4028 5573 1132
Size: 97 2799 1545 2229
Fragments arranged by size:
2799 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6670
PciI A'CATG_T
Cuts at: 5569 5569
Size: 6670
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2422 1108
Size: 1314 5356
PfoI T'CCnGG_A
Cuts at: 2128 3288 3765 2128
Size: 1160 477 5033
Fragments arranged by size:
5033 1160 477
PmeI GTTT'AAAC
Cuts at: 3974 3974
Size: 6670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4739 4771 6252 6284 4739
Size: 32 1481 32 5125
Fragments arranged by size:
5125 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2641 3099 3159 3336 2641
Size: 458 60 177 5975
Fragments arranged by size:
5975 458 177 60
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6670
PsiI TTA'TAA
Cuts at: 4240 4240
Size: 6670
PstI C_TGCA'G
Cuts at: 830 2804 3279 3335 3664 3789 3834 4022 830
Size: 1974 475 56 329 125 45 188 3478
Fragments arranged by size:
3478 1974 475 329 188 125 56 45
PvuI CG_AT'CG
Cuts at: 2001 2431 2001
Size: 430 6240
PvuII CAG'CTG
Cuts at: 2422 2799 2422
Size: 377 6293
SacI G_AGCT'C
Cuts at: 2331 3774 3903 2331
Size: 1443 129 5098
Fragments arranged by size:
5098 1443 129
SalI G'TCGA_C
Cuts at: 1936 4012 1936
Size: 2076 4594
SapI GCTCTTCn'nnn_
Cuts at: 2069 2069
Size: 6670
SbfI CC_TGCA'GG
Cuts at: 3279 4022 3279
Size: 743 5927
ScaI AGT'ACT
Cuts at: 4918 6630 4918
Size: 1712 4958
SexAI A'CCwGG_T
Cuts at: 3306 3306
Size: 6670
SfcI C'TryA_G
Cuts at: 826 1045 2800 3275 3331 3660 3785 3830 4018
Size: 219 1755 475 56 329 125 45 188
Cuts at: 4018 5153 5834 6025 826
Size: 1135 681 191 1471
Fragments arranged by size:
1755 1471 1135 681 475 329 219 191
188 125 56 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6670
SmaI CCC'GGG
Cuts at: 1872 2659 3238 3259 3512 3997 1872
Size: 787 579 21 253 485 4545
Fragments arranged by size:
4545 787 579 485 253 21
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3902 4774 5675 5937 820
Size: 197 926 1959 872 901 262 1553
Fragments arranged by size:
1959 1553 926 901 872 262 197
SnaBI TAC'GTA
Cuts at: 493 3967 4461 493
Size: 3474 494 2702
Fragments arranged by size:
3474 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6670
SphI G_CATG'C
Cuts at: 1132 4028 1132
Size: 2896 3774
SspI AAT'ATT
Cuts at: 5 52 4594 5
Size: 47 4542 2081
Fragments arranged by size:
4542 2081 47
StuI AGG'CCT
Cuts at: 1517 2567 2653 2882 3179 3959 1517
Size: 1050 86 229 297 780 4228
Fragments arranged by size:
4228 1050 780 297 229 86
StyI C'CwwG_G
Cuts at: 513 1065 2022 2217 2568 2828 2931 3111 3912
Size: 552 957 195 351 260 103 180 801
Cuts at: 3912 3943 4109 513
Size: 31 166 3074
Fragments arranged by size:
3074 957 801 552 351 260 195 180
166 103 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2168 2937 3230 3422 4697 4714 4867
Size: 1255 588 769 293 192 1275 17 153
Cuts at: 4867 5052 5497 325
Size: 185 445 1498
Fragments arranged by size:
1498 1275 1255 769 588 445 293 192
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4916 6628 96
Size: 274 80 33 51 1443 2939 1712 138
Fragments arranged by size:
2939 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2912 2944 2970 3002 6630 6662 2912
Size: 32 26 32 3628 32 2920
Fragments arranged by size:
3628 2920 32 32 32 26
Tth111I GACn'n_nGTC
Cuts at: 1316 3163 1316
Size: 1847 4823
XbaI T'CTAG_A
Cuts at: 4006 4006
Size: 6670
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2581 2581
Size: 6670
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6670
XmaI C'CCGG_G
Cuts at: 1870 2657 3236 3257 3510 3995 1870
Size: 787 579 21 253 485 4545
Fragments arranged by size:
4545 787 579 485 253 21
XmnI GAAnn'nnTTC
Cuts at: 2304 4799 2304
Size: 2495 4175
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4478 276
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI