(Circular) (Six-base) MAPSORT of: pFN21ASDA1504.seq Check: 942 from: 1 to: 6007 pFN21ASDA1504 6007 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3817 278 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2496 3350 1937 Size: 559 854 4594 Fragments arranged by size: 4594 854 559 Acc65I G'GTAC_C Cuts at: 2819 3328 2819 Size: 509 5498 AclI AA'CG_TT Cuts at: 1563 4134 4507 1563 Size: 2571 373 3063 Fragments arranged by size: 3063 2571 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4070 5454 5722 1632 Size: 2438 1384 268 1917 Fragments arranged by size: 2438 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5810 AflIII A'CryG_T Cuts at: 1203 4906 5883 1203 Size: 3703 977 1327 Fragments arranged by size: 3703 1327 977 AgeI A'CCGG_T Cuts at: 4838 4838 Size: 6007 AhdI GACnn_n'nnGTC Cuts at: 4736 4736 Size: 6007 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6007 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6007 AlwNI CAG_nnn'CTG Cuts at: 3750 5322 3750 Size: 1572 4435 ApaLI G'TGCA_C Cuts at: 4064 5220 4064 Size: 1156 4851 ApoI r'AATT_y Cuts at: 1493 2128 2269 3247 3268 3316 3542 3694 5803 Size: 635 141 978 21 48 226 152 2109 Cuts at: 5803 1493 Size: 1697 Fragments arranged by size: 2109 1697 978 635 226 152 141 48 21 AseI AT'TA_AT Cuts at: 160 1032 2558 4561 160 Size: 872 1526 2003 1606 Fragments arranged by size: 2003 1606 1526 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 AvaI C'yCGr_G Cuts at: 1870 1943 3332 1870 Size: 73 1389 4545 Fragments arranged by size: 4545 1389 73 BamHI G'GATC_C Cuts at: 3337 3704 3337 Size: 367 5640 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2819 3328 4783 618 Size: 325 217 627 1032 509 1455 1842 Fragments arranged by size: 1842 1455 1032 627 509 325 217 BanII G_rGCy'C Cuts at: 1391 2417 3326 1391 Size: 1026 909 4072 Fragments arranged by size: 4072 1026 909 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2180 3073 928 Size: 971 281 893 3862 Fragments arranged by size: 3862 971 893 281 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4198 4232 1903 Size: 34 2261 34 3678 Fragments arranged by size: 3678 2261 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3899 5109 3899 Size: 1210 4797 BclI T'GATC_A Cuts at: 1548 1548 Size: 6007 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4618 136 Size: 107 122 71 631 605 2946 1525 Fragments arranged by size: 2946 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3032 6002 3032 Size: 2970 3037 BlpI GC'TnA_GC Cuts at: 3424 3424 Size: 6007 Bme1580I G_kGCm'C Cuts at: 1790 4068 5224 1790 Size: 2278 1156 2573 Fragments arranged by size: 2573 2278 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6007 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2912 3738 4696 Size: 416 53 83 399 24 1495 826 958 Cuts at: 4696 442 Size: 1753 Fragments arranged by size: 1753 1495 958 826 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6007 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2436 2436 Size: 6007 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2568 2956 3204 4667 1239 Size: 1329 388 248 1463 2579 Fragments arranged by size: 2579 1463 1329 388 248 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4132 4997 5295 4132 Size: 865 298 4844 Fragments arranged by size: 4844 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4670 882 Size: 617 3171 2219 Fragments arranged by size: 3171 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3304 3798 493 Size: 711 2100 494 2702 Fragments arranged by size: 2702 2100 711 494 BsaBI GATnn'nnATC Cuts at: 6001 6001 Size: 6007 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2941 3814 4196 4831 Size: 53 83 186 936 1408 873 382 635 Cuts at: 4831 275 Size: 1451 Fragments arranged by size: 1451 1408 936 873 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4440 4838 5112 5259 5670 4440 Size: 398 274 147 411 4777 Fragments arranged by size: 4777 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5782 Fragments arranged by size: 5782 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2610 1186 Size: 1424 4583 BseYI C'CCAG_C Cuts at: 2054 5210 5706 2054 Size: 3156 496 2355 Fragments arranged by size: 3156 2355 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5434 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3457 4218 5246 1540 Size: 275 186 1456 761 1028 2301 Fragments arranged by size: 2301 1456 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2417 4068 4153 5224 2417 Size: 1651 85 1071 3200 Fragments arranged by size: 3200 1651 1071 85 BsmI GAATG_Cn' Cuts at: 3518 3611 3518 Size: 93 5914 BsmBI CGTCTCn'nnnn_ Cuts at: 2948 2948 Size: 6007 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2417 3326 4068 4153 5224 1391 Size: 399 627 909 742 85 1071 2174 Fragments arranged by size: 2174 1071 909 742 627 399 85 BspEI T'CCGG_A Cuts at: 5670 5670 Size: 6007 BspHI T'CATG_A Cuts at: 2095 2590 2884 3896 5626 2095 Size: 495 294 1012 1730 2476 Fragments arranged by size: 2476 1730 1012 495 294 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3362 844 Size: 2518 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3894 1394 Size: 429 2071 3507 Fragments arranged by size: 3507 2071 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4502 4676 65 Size: 1183 3254 174 1396 Fragments arranged by size: 3254 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4651 4838 1700 Size: 110 2841 187 2869 Fragments arranged by size: 2869 2841 187 110 BsrGI T'GTAC_A Cuts at: 96 2501 2652 96 Size: 2405 151 3451 Fragments arranged by size: 3451 2405 151 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6007 BssSI C'ACGA_G Cuts at: 4067 5079 4067 Size: 1012 4995 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6007 BstBI TT'CG_AA Cuts at: 2007 4811 2007 Size: 2804 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6007 BstXI CCAn_nnnn'nTGG Cuts at: 3178 3178 Size: 6007 BstYI r'GATC_y Cuts at: 1972 2698 3032 3337 3704 4089 4106 5558 5644 Size: 726 334 305 367 385 17 1452 86 Cuts at: 5644 5656 6002 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 726 385 367 346 334 305 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2484 2484 Size: 6007 BtgI C'CryG_G Cuts at: 513 1065 2984 3070 513 Size: 552 1919 86 3450 Fragments arranged by size: 3450 1919 552 86 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5268 Fragments arranged by size: 5268 683 56 BtsI GCAGTG_nn' Cuts at: 3523 4317 4337 3523 Size: 794 20 5193 Fragments arranged by size: 5193 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6007 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5972 DraI TTT'AAA Cuts at: 3311 3311 Size: 6007 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2641 5014 809 Size: 507 1325 2373 1802 Fragments arranged by size: 2373 1802 1325 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3454 4343 5972 Size: 54 1425 50 275 18 1624 889 1629 Cuts at: 5972 8 Size: 43 Fragments arranged by size: 1629 1624 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3454 1537 Size: 275 1642 4090 Fragments arranged by size: 4090 1642 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2811 2984 3937 1353 Size: 1458 173 953 3423 Fragments arranged by size: 3423 1458 953 173 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4594 4968 5114 387 Size: 1064 138 3005 374 146 1280 Fragments arranged by size: 3005 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2415 2415 Size: 6007 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2568 2956 3204 4070 4667 5454 5722 Size: 393 936 388 248 866 597 787 268 Cuts at: 5722 1239 Size: 1524 Fragments arranged by size: 1524 936 866 787 597 393 388 268 248 EcoO109I rG'GnC_Cy Cuts at: 2051 2051 Size: 6007 EcoRI G'AATT_C Cuts at: 2269 3316 3694 2269 Size: 1047 378 4582 Fragments arranged by size: 4582 1047 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2204 2946 2204 Size: 742 5265 FspI TGC'GCA Cuts at: 4513 4513 Size: 6007 HaeII r_GCGC'y Cuts at: 1678 5154 1678 Size: 3476 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4648 4680 4722 4754 1373 Size: 32 135 32 3076 32 42 32 2626 Fragments arranged by size: 3076 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2971 3351 3597 669 Size: 988 281 1033 380 246 3079 Fragments arranged by size: 3079 1033 988 380 281 246 HindIII A'AGCT_T Cuts at: 748 2847 748 Size: 2099 3908 HpaI GTT'AAC Cuts at: 2971 3597 2971 Size: 626 5381 KpnI G_GTAC'C Cuts at: 2823 3332 2823 Size: 509 5498 MfeI C'AATT_G Cuts at: 3606 3606 Size: 6007 MluI A'CGCG_T Cuts at: 5883 5883 Size: 6007 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2405 3008 5121 5305 1305 Size: 1100 603 2113 184 2007 Fragments arranged by size: 2113 2007 1100 603 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4185 Fragments arranged by size: 4185 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3237 3965 4324 4483 518 Size: 648 2071 728 359 159 2042 Fragments arranged by size: 2071 2042 728 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3423 4100 5248 5493 1606 Size: 1817 677 1148 245 2120 Fragments arranged by size: 2120 1817 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2984 3070 513 Size: 552 1919 86 3450 Fragments arranged by size: 3450 1919 552 86 NdeI CA'TA_TG Cuts at: 387 387 Size: 6007 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6007 NotI GC'GGCC_GC Cuts at: 3454 3454 Size: 6007 NspI r_CATG'y Cuts at: 1132 1229 3365 4910 1132 Size: 97 2136 1545 2229 Fragments arranged by size: 2229 2136 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6007 PciI A'CATG_T Cuts at: 4906 4906 Size: 6007 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6007 PfoI T'CCnGG_A Cuts at: 2933 2933 Size: 6007 PmeI GTTT'AAAC Cuts at: 3311 3311 Size: 6007 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4076 4108 5589 5621 4076 Size: 32 1481 32 4462 Fragments arranged by size: 4462 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2051 2051 Size: 6007 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6007 PsiI TTA'TAA Cuts at: 2679 3577 2679 Size: 898 5109 PstI C_TGCA'G Cuts at: 830 3359 830 Size: 2529 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6007 SacI G_AGCT'C Cuts at: 2417 2417 Size: 6007 SalI G'TCGA_C Cuts at: 1936 3349 1936 Size: 1413 4594 SanDI GG'GwC_CC Cuts at: 2051 2051 Size: 6007 SbfI CC_TGCA'GG Cuts at: 3359 3359 Size: 6007 ScaI AGT'ACT Cuts at: 4255 5967 4255 Size: 1712 4295 SfcI C'TryA_G Cuts at: 826 1045 2964 3355 4490 5171 5362 826 Size: 219 1919 391 1135 681 191 1471 Fragments arranged by size: 1919 1471 1135 681 391 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6007 SmaI CCC'GGG Cuts at: 1872 3334 1872 Size: 1462 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4111 5012 5274 820 Size: 197 926 2168 901 262 1553 Fragments arranged by size: 2168 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3304 3798 493 Size: 2811 494 2702 Fragments arranged by size: 2811 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6007 SphI G_CATG'C Cuts at: 1132 3365 1132 Size: 2233 3774 SspI AAT'ATT Cuts at: 5 52 3931 5 Size: 47 3879 2081 Fragments arranged by size: 3879 2081 47 StuI AGG'CCT Cuts at: 1517 3286 1517 Size: 1769 4238 StyI C'CwwG_G Cuts at: 513 1065 2984 3070 3446 513 Size: 552 1919 86 376 3074 Fragments arranged by size: 3074 1919 552 376 86 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3079 4034 4051 4204 4389 4834 325 Size: 1255 1499 955 17 153 185 445 1498 Fragments arranged by size: 1499 1498 1255 955 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2501 2652 4253 Size: 274 80 33 51 1443 524 151 1601 Cuts at: 4253 5965 96 Size: 1712 138 Fragments arranged by size: 1712 1601 1443 524 274 151 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5967 5999 5967 Size: 32 5975 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6007 XbaI T'CTAG_A Cuts at: 3343 3343 Size: 6007 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6007 XmaI C'CCGG_G Cuts at: 1870 3332 1870 Size: 1462 4545 XmnI GAAnn'nnTTC Cuts at: 2103 2929 4136 2103 Size: 826 1207 3974 Fragments arranged by size: 3974 1207 826 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3815 276 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI