(Circular) (Six-base) MAPSORT of: pFN21ASDA1490.seq Check: 3767 from: 1 to: 6973 pFN21ASDA1490 6973 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4226 4226 Size: 6973 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4783 278 Size: 53 83 186 936 3247 2468 Fragments arranged by size: 3247 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4316 1937 Size: 2379 4594 Acc65I G'GTAC_C Cuts at: 4294 4294 Size: 6973 AclI AA'CG_TT Cuts at: 1563 5100 5473 1563 Size: 3537 373 3063 Fragments arranged by size: 3537 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2071 2598 2602 2882 5036 6420 6688 1632 Size: 439 527 4 280 2154 1384 268 1917 Fragments arranged by size: 2154 1917 1384 527 439 280 268 4 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6776 AflIII A'CryG_T Cuts at: 1203 3906 3953 5872 6849 1203 Size: 2703 47 1919 977 1327 Fragments arranged by size: 2703 1919 1327 977 47 AgeI A'CCGG_T Cuts at: 5804 5804 Size: 6973 AhdI GACnn_n'nnGTC Cuts at: 5702 5702 Size: 6973 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6973 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3527 1184 Size: 2343 4630 AlwNI CAG_nnn'CTG Cuts at: 2033 4716 6288 2033 Size: 2683 1572 2718 Fragments arranged by size: 2718 2683 1572 ApaLI G'TGCA_C Cuts at: 5030 6186 5030 Size: 1156 5817 ApoI r'AATT_y Cuts at: 1493 2582 2970 4282 4508 4660 6769 1493 Size: 1089 388 1312 226 152 2109 1697 Fragments arranged by size: 2109 1697 1312 1089 388 226 152 AseI AT'TA_AT Cuts at: 160 1032 5527 160 Size: 872 4495 1606 Fragments arranged by size: 4495 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6973 AvaI C'yCGr_G Cuts at: 1870 1943 4298 1870 Size: 73 2355 4545 Fragments arranged by size: 4545 2355 73 BamHI G'GATC_C Cuts at: 3480 3742 4303 4670 3480 Size: 262 561 367 5783 Fragments arranged by size: 5783 561 367 262 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2303 2410 4238 4294 5749 Size: 325 217 627 516 107 1828 56 1455 Cuts at: 5749 618 Size: 1842 Fragments arranged by size: 1842 1828 1455 627 516 325 217 107 56 BanII G_rGCy'C Cuts at: 1391 2344 2466 3096 4292 1391 Size: 953 122 630 1196 4072 Fragments arranged by size: 4072 1196 953 630 122 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6002 BbvCI CC'TCA_GC Cuts at: 3087 3087 Size: 6973 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2714 2748 5164 5198 1903 Size: 34 777 34 2416 34 3678 Fragments arranged by size: 3678 2416 777 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4865 6075 4865 Size: 1210 5763 BclI T'GATC_A Cuts at: 1548 1548 Size: 6973 BfrBI ATG'CAT Cuts at: 3000 3155 3275 3000 Size: 155 120 6698 Fragments arranged by size: 6698 155 120 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3708 5584 136 Size: 107 122 71 631 605 2036 1876 1525 Fragments arranged by size: 2036 1876 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6968 6968 Size: 6973 BlpI GC'TnA_GC Cuts at: 2607 4390 2607 Size: 1783 5190 Bme1580I G_kGCm'C Cuts at: 1790 2357 5034 6190 1790 Size: 567 2677 1156 2573 Fragments arranged by size: 2677 2573 1156 567 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6973 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2176 4704 5662 Size: 416 53 83 399 24 759 2528 958 Cuts at: 5662 442 Size: 1753 Fragments arranged by size: 2528 1753 958 759 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6973 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2551 3733 5633 1239 Size: 1312 1182 1900 2579 Fragments arranged by size: 2579 1900 1312 1182 Bpu10I CC'TnA_GC Cuts at: 3087 3203 3087 Size: 116 6857 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2976 5098 5963 6261 2976 Size: 2122 865 298 3688 Fragments arranged by size: 3688 2122 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5636 882 Size: 617 4137 2219 Fragments arranged by size: 4137 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2386 4270 4764 493 Size: 711 1182 1884 494 2702 Fragments arranged by size: 2702 1884 1182 711 494 BsaBI GATnn'nnATC Cuts at: 6967 6967 Size: 6973 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4780 5162 5797 275 Size: 53 83 186 936 3247 382 635 1451 Fragments arranged by size: 3247 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5406 5804 6078 6225 6636 5406 Size: 398 274 147 411 5743 Fragments arranged by size: 5743 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3922 3952 4222 4252 1178 Size: 30 165 30 2519 30 270 30 3899 Fragments arranged by size: 3899 2519 270 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2233 2269 2414 2417 2789 3104 3943 1186 Size: 1047 36 145 3 372 315 839 4216 Fragments arranged by size: 4216 1047 839 372 315 145 36 3 BseYI C'CCAG_C Cuts at: 2174 6176 6672 2174 Size: 4002 496 2475 Fragments arranged by size: 4002 2475 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2679 1163 Size: 573 943 5457 Fragments arranged by size: 5457 943 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4423 5184 6212 1540 Size: 275 186 2422 761 1028 2301 Fragments arranged by size: 2422 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2166 2222 2466 3096 3797 3916 5034 5119 6190 Size: 56 244 630 701 119 1118 85 1071 Cuts at: 6190 2166 Size: 2949 Fragments arranged by size: 2949 1118 1071 701 630 244 119 85 56 BsmI GAATG_Cn' Cuts at: 4484 4577 4484 Size: 93 6880 BsmBI CGTCTCn'nnnn_ Cuts at: 2103 2103 Size: 6973 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2166 2222 2344 2357 2466 3096 3797 Size: 399 376 56 122 13 109 630 701 Cuts at: 3797 3916 4292 5034 5119 6190 1391 Size: 119 376 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 701 630 399 376 376 122 119 109 85 56 13 BspEI T'CCGG_A Cuts at: 6636 6636 Size: 6973 BspHI T'CATG_A Cuts at: 2937 3985 4862 6592 2937 Size: 1048 877 1730 3318 Fragments arranged by size: 3318 1730 1048 877 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2701 4226 4328 844 Size: 1857 1525 102 3489 Fragments arranged by size: 3489 1857 1525 102 BsrBI CCG'CTC Cuts at: 1394 1823 4860 1394 Size: 429 3037 3507 Fragments arranged by size: 3507 3037 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3202 3327 5468 5642 65 Size: 1183 1954 125 2141 174 1396 Fragments arranged by size: 2141 1954 1396 1183 174 125 BsrFI r'CCGG_y Cuts at: 1700 1810 2108 2531 5617 5804 1700 Size: 110 298 423 3086 187 2869 Fragments arranged by size: 3086 2869 423 298 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6973 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6973 BssSI C'ACGA_G Cuts at: 2323 5033 6045 2323 Size: 2710 1012 3251 Fragments arranged by size: 3251 2710 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2065 794 Size: 1271 5702 BstBI TT'CG_AA Cuts at: 2007 5777 2007 Size: 3770 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6973 BstYI r'GATC_y Cuts at: 1972 3480 3742 4303 4670 5055 5072 6524 6610 Size: 1508 262 561 367 385 17 1452 86 Cuts at: 6610 6622 6968 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1508 1452 561 385 367 346 262 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 4065 513 Size: 552 3000 3421 Fragments arranged by size: 3421 3000 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6234 Fragments arranged by size: 6234 683 56 BtsI GCAGTG_nn' Cuts at: 2155 3233 4489 5283 5303 2155 Size: 1078 1256 794 20 3825 Fragments arranged by size: 3825 1256 1078 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6973 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3234 3269 529 Size: 35 2670 35 4233 Fragments arranged by size: 4233 2670 35 35 DraI TTT'AAA Cuts at: 4277 4277 Size: 6973 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5980 809 Size: 507 4664 1802 Fragments arranged by size: 4664 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2533 2736 3108 Size: 54 1425 50 275 18 703 203 372 Cuts at: 3108 3948 4420 5309 6938 8 Size: 840 472 889 1629 43 Fragments arranged by size: 1629 1425 889 840 703 472 372 275 203 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4420 1537 Size: 275 2608 4090 Fragments arranged by size: 4090 2608 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2090 2251 2257 2278 2287 2384 4903 1353 Size: 737 161 6 21 9 97 2519 3423 Fragments arranged by size: 3423 2519 737 161 97 21 9 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5560 5934 6080 387 Size: 1064 138 3971 374 146 1280 Fragments arranged by size: 3971 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2464 3094 2464 Size: 630 6343 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2071 2551 2598 2602 2882 3733 5036 Size: 393 439 480 47 4 280 851 1303 Cuts at: 5036 5633 6420 6688 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1303 851 787 597 480 439 393 280 268 47 4 EcoNI CCTnn'n_nnAGG Cuts at: 2138 2138 Size: 6973 EcoO109I rG'GnC_Cy Cuts at: 2171 2815 3680 2171 Size: 644 865 5464 Fragments arranged by size: 5464 865 644 EcoRI G'AATT_C Cuts at: 2582 2970 4282 4660 2582 Size: 388 1312 378 4895 Fragments arranged by size: 4895 1312 388 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3369 3369 Size: 6973 FspI TGC'GCA Cuts at: 5479 5479 Size: 6973 HaeII r_GCGC'y Cuts at: 1678 6120 1678 Size: 4442 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2549 2581 3457 3489 3924 Size: 32 135 32 977 32 876 32 435 Cuts at: 3924 3956 5614 5646 5688 5720 1373 Size: 32 1658 32 42 32 2626 Fragments arranged by size: 2626 1658 977 876 435 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3642 3755 4317 4563 669 Size: 988 281 1704 113 562 246 3079 Fragments arranged by size: 3079 1704 988 562 281 246 113 HindIII A'AGCT_T Cuts at: 748 2942 748 Size: 2194 4779 HpaI GTT'AAC Cuts at: 4563 4563 Size: 6973 KpnI G_GTAC'C Cuts at: 4298 4298 Size: 6973 MfeI C'AATT_G Cuts at: 3783 4572 3783 Size: 789 6184 MluI A'CGCG_T Cuts at: 6849 6849 Size: 6973 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2748 3376 3817 3922 6087 6271 1305 Size: 1443 628 441 105 2165 184 2007 Fragments arranged by size: 2165 2007 1443 628 441 184 105 MscI TGG'CCA Cuts at: 10 64 1489 1832 3110 3950 10 Size: 54 1425 343 1278 840 3033 Fragments arranged by size: 3033 1425 1278 840 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3651 4931 5290 5449 518 Size: 648 2485 1280 359 159 2042 Fragments arranged by size: 2485 2042 1280 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2076 2296 3086 4389 5066 6214 6459 1606 Size: 470 220 790 1303 677 1148 245 2120 Fragments arranged by size: 2120 1303 1148 790 677 470 245 220 NcoI C'CATG_G Cuts at: 513 1065 4065 513 Size: 552 3000 3421 Fragments arranged by size: 3421 3000 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6973 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6973 NotI GC'GGCC_GC Cuts at: 4420 4420 Size: 6973 NsiI A_TGCA'T Cuts at: 3002 3157 3277 3002 Size: 155 120 6698 Fragments arranged by size: 6698 155 120 NspI r_CATG'y Cuts at: 1132 1229 3184 3211 3910 3957 4331 5876 1132 Size: 97 1955 27 699 47 374 1545 2229 Fragments arranged by size: 2229 1955 1545 699 374 97 47 27 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6973 PciI A'CATG_T Cuts at: 3906 3953 5872 3906 Size: 47 1919 5007 Fragments arranged by size: 5007 1919 47 PflMI CCAn_nnn'nTGG Cuts at: 1108 3618 1108 Size: 2510 4463 PfoI T'CCnGG_A Cuts at: 2428 2428 Size: 6973 PmeI GTTT'AAAC Cuts at: 4277 4277 Size: 6973 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5042 5074 6555 6587 5042 Size: 32 1481 32 5428 Fragments arranged by size: 5428 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2171 2815 2171 Size: 644 6329 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6973 PsiI TTA'TAA Cuts at: 3224 4543 3224 Size: 1319 5654 PstI C_TGCA'G Cuts at: 830 2334 2887 3516 4325 830 Size: 1504 553 629 809 3478 Fragments arranged by size: 3478 1504 809 629 553 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6973 PvuII CAG'CTG Cuts at: 3086 3086 Size: 6973 SacI G_AGCT'C Cuts at: 2466 3096 2466 Size: 630 6343 SalI G'TCGA_C Cuts at: 1936 4315 1936 Size: 2379 4594 SanDI GG'GwC_CC Cuts at: 2171 2171 Size: 6973 SbfI CC_TGCA'GG Cuts at: 4325 4325 Size: 6973 ScaI AGT'ACT Cuts at: 3672 5221 6933 3672 Size: 1549 1712 3712 Fragments arranged by size: 3712 1712 1549 SfcI C'TryA_G Cuts at: 826 1045 2330 2883 3390 3411 3447 3512 4321 Size: 219 1285 553 507 21 36 65 809 Cuts at: 4321 5456 6137 6328 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1285 1135 809 681 553 507 219 191 65 36 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6973 SgrAI Cr'CCGG_yG Cuts at: 1700 2108 1700 Size: 408 6565 SmaI CCC'GGG Cuts at: 1872 4300 1872 Size: 2428 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2991 5077 5978 6240 820 Size: 197 926 1048 2086 901 262 1553 Fragments arranged by size: 2086 1553 1048 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4270 4764 493 Size: 3777 494 2702 Fragments arranged by size: 3777 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6973 SphI G_CATG'C Cuts at: 1132 3211 4331 1132 Size: 2079 1120 3774 Fragments arranged by size: 3774 2079 1120 SspI AAT'ATT Cuts at: 5 52 3878 4225 4897 5 Size: 47 3826 347 672 2081 Fragments arranged by size: 3826 2081 672 347 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6973 StyI C'CwwG_G Cuts at: 513 1065 4065 4114 4412 513 Size: 552 3000 49 298 3074 Fragments arranged by size: 3074 3000 552 298 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5000 5017 5170 5355 5800 325 Size: 1255 3420 17 153 185 445 1498 Fragments arranged by size: 3420 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3670 5219 6931 Size: 274 80 33 51 1443 1693 1549 1712 Cuts at: 6931 96 Size: 138 Fragments arranged by size: 1712 1693 1549 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2296 2328 3895 3922 3927 3954 4222 4254 6933 Size: 32 1567 27 5 27 268 32 2679 Cuts at: 6933 6965 2296 Size: 32 2304 Fragments arranged by size: 2679 2304 1567 268 32 32 32 27 27 5 Tth111I GACn'n_nGTC Cuts at: 1316 2819 3654 1316 Size: 1503 835 4635 Fragments arranged by size: 4635 1503 835 XbaI T'CTAG_A Cuts at: 3313 4309 3313 Size: 996 5977 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6973 XmaI C'CCGG_G Cuts at: 1870 4298 1870 Size: 2428 4545 XmnI GAAnn'nnTTC Cuts at: 2084 5102 2084 Size: 3018 3955 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4781 276 Size: 53 83 186 936 3247 2468 Fragments arranged by size: 3247 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI