(Circular) (Six-base) MAPSORT of: pFN21ASDA1479.seq Check: 3523 from: 1 to: 7762 pFN21ASDA1479 7762 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3863 3863 Size: 7762 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5572 278 Size: 53 83 186 936 4036 2468 Fragments arranged by size: 4036 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2121 3885 4163 5105 1937 Size: 184 1764 278 942 4594 Fragments arranged by size: 4594 1764 942 278 184 Acc65I G'GTAC_C Cuts at: 2432 5083 2432 Size: 2651 5111 AclI AA'CG_TT Cuts at: 1563 5889 6262 1563 Size: 4326 373 3063 Fragments arranged by size: 4326 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2115 2151 3372 3732 4505 5825 7209 7477 Size: 483 36 1221 360 773 1320 1384 268 Cuts at: 7477 1632 Size: 1917 Fragments arranged by size: 1917 1384 1320 1221 773 483 360 268 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7565 AflIII A'CryG_T Cuts at: 1203 3900 4350 6661 7638 1203 Size: 2697 450 2311 977 1327 Fragments arranged by size: 2697 2311 1327 977 450 AgeI A'CCGG_T Cuts at: 6593 6593 Size: 7762 AhdI GACnn_n'nnGTC Cuts at: 6491 6491 Size: 7762 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7762 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3904 1184 Size: 2720 5042 AlwNI CAG_nnn'CTG Cuts at: 2588 3077 3564 5505 7077 2588 Size: 489 487 1941 1572 3273 Fragments arranged by size: 3273 1941 1572 489 487 ApaLI G'TGCA_C Cuts at: 5819 6975 5819 Size: 1156 6606 ApoI r'AATT_y Cuts at: 1493 2214 3734 4735 5071 5297 5449 7558 1493 Size: 721 1520 1001 336 226 152 2109 1697 Fragments arranged by size: 2109 1697 1520 1001 721 336 226 152 AseI AT'TA_AT Cuts at: 160 1032 6316 160 Size: 872 5284 1606 Fragments arranged by size: 5284 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7762 AvaI C'yCGr_G Cuts at: 1870 1943 3267 5087 1870 Size: 73 1324 1820 4545 Fragments arranged by size: 4545 1820 1324 73 AvrII C'CTAG_G Cuts at: 4949 4949 Size: 7762 BamHI G'GATC_C Cuts at: 3638 4564 5092 5459 3638 Size: 926 528 367 5941 Fragments arranged by size: 5941 926 528 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2432 5083 6538 618 Size: 325 217 627 645 2651 1455 1842 Fragments arranged by size: 2651 1842 1455 645 627 325 217 BanII G_rGCy'C Cuts at: 1391 3252 5081 1391 Size: 1861 1829 4072 Fragments arranged by size: 4072 1861 1829 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2023 3105 3477 3687 4190 928 Size: 971 124 1082 372 210 503 4500 Fragments arranged by size: 4500 1082 971 503 372 210 124 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5953 5987 1903 Size: 34 4016 34 3678 Fragments arranged by size: 4016 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5654 6864 5654 Size: 1210 6552 BclI T'GATC_A Cuts at: 1548 4405 1548 Size: 2857 4905 BfrBI ATG'CAT Cuts at: 2441 2441 Size: 7762 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2169 2585 6373 Size: 107 122 71 631 605 497 416 3788 Cuts at: 6373 136 Size: 1525 Fragments arranged by size: 3788 1525 631 605 497 416 122 107 71 BglII A'GATC_T Cuts at: 7757 7757 Size: 7762 BlpI GC'TnA_GC Cuts at: 5179 5179 Size: 7762 Bme1580I G_kGCm'C Cuts at: 1790 5823 6979 1790 Size: 4033 1156 2573 Fragments arranged by size: 4033 2573 1156 BmgBI CAC'GTC Cuts at: 1318 4353 4990 1318 Size: 3035 637 4090 Fragments arranged by size: 4090 3035 637 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2065 3943 4305 Size: 416 53 83 399 24 648 1878 362 Cuts at: 4305 4860 5493 6451 442 Size: 555 633 958 1753 Fragments arranged by size: 1878 1753 958 648 633 555 416 399 362 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7762 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2852 2904 3054 3813 3949 4116 4710 4897 Size: 1613 52 150 759 136 167 594 187 Cuts at: 4897 6422 1239 Size: 1525 2579 Fragments arranged by size: 2579 1613 1525 759 594 187 167 150 136 52 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5887 6752 7050 5887 Size: 865 298 6599 Fragments arranged by size: 6599 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3998 6425 882 Size: 617 2499 2427 2219 Fragments arranged by size: 2499 2427 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5059 5553 493 Size: 711 3855 494 2702 Fragments arranged by size: 3855 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3504 7756 3504 Size: 4252 3510 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2161 4970 5569 5951 Size: 53 83 186 936 628 2809 599 382 Cuts at: 5951 6586 275 Size: 635 1451 Fragments arranged by size: 2809 1451 936 635 628 599 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2146 6195 6593 6867 7014 7425 2146 Size: 4049 398 274 147 411 2483 Fragments arranged by size: 4049 2483 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7537 Fragments arranged by size: 7537 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7762 BseYI C'CCAG_C Cuts at: 2820 6965 7461 2820 Size: 4145 496 3121 Fragments arranged by size: 4145 3121 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3472 1163 Size: 573 1736 5453 Fragments arranged by size: 5453 1736 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5212 5973 7001 1540 Size: 275 186 3211 761 1028 2301 Fragments arranged by size: 3211 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5823 5908 6979 5823 Size: 85 1071 6606 Fragments arranged by size: 6606 1071 85 BsmI GAATG_Cn' Cuts at: 2378 2441 3863 5273 5366 2378 Size: 63 1422 1410 93 4774 Fragments arranged by size: 4774 1422 1410 93 63 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3252 5081 5823 5908 6979 1391 Size: 399 1462 1829 742 85 1071 2174 Fragments arranged by size: 2174 1829 1462 1071 742 399 85 BspEI T'CCGG_A Cuts at: 7425 7425 Size: 7762 BspHI T'CATG_A Cuts at: 5651 7381 5651 Size: 1730 6032 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3863 4065 5117 844 Size: 3019 202 1052 3489 Fragments arranged by size: 3489 3019 1052 202 BsrBI CCG'CTC Cuts at: 1394 1823 5649 1394 Size: 429 3826 3507 Fragments arranged by size: 3826 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2179 3237 3867 6257 6431 65 Size: 1183 931 1058 630 2390 174 1396 Fragments arranged by size: 2390 1396 1183 1058 931 630 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4443 6406 6593 1700 Size: 110 2633 1963 187 2869 Fragments arranged by size: 2869 2633 1963 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7762 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7762 BssSI C'ACGA_G Cuts at: 3606 5822 6834 3606 Size: 2216 1012 4534 Fragments arranged by size: 4534 2216 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3869 794 Size: 3075 4687 BstBI TT'CG_AA Cuts at: 2007 6566 2007 Size: 4559 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7762 BstXI CCAn_nnnn'nTGG Cuts at: 3298 3808 4111 4229 4669 3298 Size: 510 303 118 440 6391 Fragments arranged by size: 6391 510 440 303 118 BstYI r'GATC_y Cuts at: 1972 2636 3638 3924 4564 5092 5459 5844 5861 Size: 664 1002 286 640 528 367 385 17 Cuts at: 5861 7313 7399 7411 7757 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1002 664 640 528 385 367 346 286 86 17 12 BstZ17I GTA'TAC Cuts at: 3886 3886 Size: 7762 BtgI C'CryG_G Cuts at: 513 1065 3766 4104 4222 4228 4569 4825 513 Size: 552 2701 338 118 6 341 256 3450 Fragments arranged by size: 3450 2701 552 341 338 256 118 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2619 4602 487 Size: 683 56 1393 1983 3647 Fragments arranged by size: 3647 1983 1393 683 56 BtsI GCAGTG_nn' Cuts at: 3460 5278 6072 6092 3460 Size: 1818 794 20 5130 Fragments arranged by size: 5130 1818 794 20 ClaI AT'CG_AT Cuts at: 1554 3171 4577 1554 Size: 1617 1406 4739 Fragments arranged by size: 4739 1617 1406 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7727 DraI TTT'AAA Cuts at: 5066 5066 Size: 7762 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3479 4007 6769 809 Size: 507 2163 528 2762 1802 Fragments arranged by size: 2762 2163 1802 528 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2598 3288 4225 Size: 54 1425 50 275 18 768 690 937 Cuts at: 4225 5209 6098 7727 8 Size: 984 889 1629 43 Fragments arranged by size: 1629 1425 984 937 889 768 690 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5209 1537 Size: 275 3397 4090 Fragments arranged by size: 4090 3397 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3417 4152 4196 4950 5692 1353 Size: 2064 735 44 754 742 3423 Fragments arranged by size: 3423 2064 754 742 735 44 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3934 4263 6349 6723 6869 387 Size: 1064 138 2345 329 2086 374 146 1280 Fragments arranged by size: 2345 2086 1280 1064 374 329 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2151 2852 2904 3054 3372 3732 Size: 393 483 36 701 52 150 318 360 Cuts at: 3732 3813 3949 4116 4505 4710 4897 5825 6422 Size: 81 136 167 389 205 187 928 597 Cuts at: 6422 7209 7477 1239 Size: 787 268 1524 Fragments arranged by size: 1524 928 787 701 597 483 393 389 360 318 268 205 187 167 150 136 81 52 36 EcoNI CCTnn'n_nnAGG Cuts at: 4744 4744 Size: 7762 EcoO109I rG'GnC_Cy Cuts at: 2827 4588 2827 Size: 1761 6001 EcoRI G'AATT_C Cuts at: 3734 5071 5449 3734 Size: 1337 378 6047 Fragments arranged by size: 6047 1337 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5023 5023 Size: 7762 FspI TGC'GCA Cuts at: 6268 6268 Size: 7762 HaeII r_GCGC'y Cuts at: 1678 6909 1678 Size: 5231 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3768 3800 3926 3958 4085 Size: 32 135 32 2196 32 126 32 127 Cuts at: 4085 4117 4659 4691 4879 4911 6403 6435 6477 Size: 32 542 32 188 32 1492 32 42 Cuts at: 6477 6509 1373 Size: 32 2626 Fragments arranged by size: 2626 2196 1492 542 188 135 127 126 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2122 3675 5106 5352 669 Size: 988 281 184 1553 1431 246 3079 Fragments arranged by size: 3079 1553 1431 988 281 246 184 HindIII A'AGCT_T Cuts at: 748 3153 4391 748 Size: 2405 1238 4119 Fragments arranged by size: 4119 2405 1238 HpaI GTT'AAC Cuts at: 3675 5352 3675 Size: 1677 6085 KpnI G_GTAC'C Cuts at: 2436 5087 2436 Size: 2651 5111 MfeI C'AATT_G Cuts at: 5361 5361 Size: 7762 MluI A'CGCG_T Cuts at: 7638 7638 Size: 7762 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3477 3825 4049 6876 7060 1305 Size: 2172 348 224 2827 184 2007 Fragments arranged by size: 2827 2172 2007 348 224 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2600 4227 10 Size: 54 1425 343 768 1627 3545 Fragments arranged by size: 3545 1627 1425 768 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2367 3296 3819 3933 4109 4227 4494 Size: 648 1201 929 523 114 176 118 267 Cuts at: 4494 4538 5720 6079 6238 518 Size: 44 1182 359 159 2042 Fragments arranged by size: 2042 1201 1182 929 648 523 359 267 176 159 118 114 44 MspA1I CmG'CkG Cuts at: 1606 2203 2824 5178 5855 7003 7248 1606 Size: 597 621 2354 677 1148 245 2120 Fragments arranged by size: 2354 2120 1148 677 621 597 245 NcoI C'CATG_G Cuts at: 513 1065 3766 4104 4222 4228 4569 513 Size: 552 2701 338 118 6 341 3706 Fragments arranged by size: 3706 2701 552 341 338 118 6 NdeI CA'TA_TG Cuts at: 387 2795 387 Size: 2408 5354 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7762 NotI GC'GGCC_GC Cuts at: 5209 5209 Size: 7762 NsiI A_TGCA'T Cuts at: 2443 2443 Size: 7762 NspI r_CATG'y Cuts at: 1132 1229 3904 5120 6665 1132 Size: 97 2675 1216 1545 2229 Fragments arranged by size: 2675 2229 1545 1216 97 PasI CC'CwG_GG Cuts at: 1348 3659 1348 Size: 2311 5451 PciI A'CATG_T Cuts at: 3900 6661 3900 Size: 2761 5001 PflMI CCAn_nnn'nTGG Cuts at: 1108 3122 4088 1108 Size: 2014 966 4782 Fragments arranged by size: 4782 2014 966 PfoI T'CCnGG_A Cuts at: 2829 3177 4687 2829 Size: 348 1510 5904 Fragments arranged by size: 5904 1510 348 PmeI GTTT'AAAC Cuts at: 5066 5066 Size: 7762 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3239 3271 5831 5863 7344 7376 3239 Size: 32 2560 32 1481 32 3625 Fragments arranged by size: 3625 2560 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2827 4588 2827 Size: 1761 6001 PshAI GACnn'nnGTC Cuts at: 1613 3269 1613 Size: 1656 6106 PsiI TTA'TAA Cuts at: 3159 5332 3159 Size: 2173 5589 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3357 3389 3357 Size: 32 7730 PstI C_TGCA'G Cuts at: 830 2916 4194 4902 5114 830 Size: 2086 1278 708 212 3478 Fragments arranged by size: 3478 2086 1278 708 212 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7762 PvuII CAG'CTG Cuts at: 2203 2203 Size: 7762 SalI G'TCGA_C Cuts at: 1936 2120 5104 1936 Size: 184 2984 4594 Fragments arranged by size: 4594 2984 184 SapI GCTCTTCn'nnn_ Cuts at: 4152 4196 4152 Size: 44 7718 SbfI CC_TGCA'GG Cuts at: 4194 5114 4194 Size: 920 6842 ScaI AGT'ACT Cuts at: 6010 7722 6010 Size: 1712 6050 SfcI C'TryA_G Cuts at: 826 1045 2464 2912 4190 4898 5110 6245 6926 Size: 219 1419 448 1278 708 212 1135 681 Cuts at: 6926 7117 826 Size: 191 1471 Fragments arranged by size: 1471 1419 1278 1135 708 681 448 219 212 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7762 SgrAI Cr'CCGG_yG Cuts at: 1700 4443 1700 Size: 2743 5019 SmaI CCC'GGG Cuts at: 1872 5089 1872 Size: 3217 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5866 6767 7029 820 Size: 197 926 3923 901 262 1553 Fragments arranged by size: 3923 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5059 5553 493 Size: 4566 494 2702 Fragments arranged by size: 4566 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7762 SphI G_CATG'C Cuts at: 1132 5120 1132 Size: 3988 3774 SspI AAT'ATT Cuts at: 5 52 4030 5686 5 Size: 47 3978 1656 2081 Fragments arranged by size: 3978 2081 1656 47 StuI AGG'CCT Cuts at: 1517 3121 1517 Size: 1604 6158 StyI C'CwwG_G Cuts at: 513 1065 3116 3766 4104 4222 4228 4569 4949 Size: 552 2051 650 338 118 6 341 380 Cuts at: 4949 5201 513 Size: 252 3074 Fragments arranged by size: 3074 2051 650 552 380 341 338 252 118 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2618 2801 3250 4099 5789 5806 5959 Size: 1255 1038 183 449 849 1690 17 153 Cuts at: 5959 6144 6589 325 Size: 185 445 1498 Fragments arranged by size: 1690 1498 1255 1038 849 449 445 185 183 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6008 7720 96 Size: 274 80 33 51 1443 4031 1712 138 Fragments arranged by size: 4031 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4584 4616 4721 4753 4869 4901 7722 7754 4584 Size: 32 105 32 116 32 2821 32 4592 Fragments arranged by size: 4592 2821 116 105 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4972 1316 Size: 3656 4106 XbaI T'CTAG_A Cuts at: 5098 5098 Size: 7762 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3078 3641 4522 3078 Size: 563 881 6318 Fragments arranged by size: 6318 881 563 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7762 XmaI C'CCGG_G Cuts at: 1870 5087 1870 Size: 3217 4545 XmnI GAAnn'nnTTC Cuts at: 5891 5891 Size: 7762 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5570 276 Size: 53 83 186 936 4036 2468 Fragments arranged by size: 4036 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI