(Circular) (Six-base) MAPSORT of: pFN21ASDA1473.seq Check: 6156 from: 1 to: 6322 pFN21ASDA1473 6322 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4132 278 Size: 53 83 186 936 2596 2468 Fragments arranged by size: 2596 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3665 1937 Size: 1728 4594 Acc65I G'GTAC_C Cuts at: 3643 3643 Size: 6322 AclI AA'CG_TT Cuts at: 1563 4449 4822 1563 Size: 2886 373 3063 Fragments arranged by size: 3063 2886 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4385 5769 6037 1632 Size: 2753 1384 268 1917 Fragments arranged by size: 2753 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6125 AflIII A'CryG_T Cuts at: 1203 5221 6198 1203 Size: 4018 977 1327 Fragments arranged by size: 4018 1327 977 AgeI A'CCGG_T Cuts at: 5153 5153 Size: 6322 AhdI GACnn_n'nnGTC Cuts at: 5051 5051 Size: 6322 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6322 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6322 AlwNI CAG_nnn'CTG Cuts at: 4065 5637 4065 Size: 1572 4750 ApaLI G'TGCA_C Cuts at: 2300 4379 5535 2300 Size: 2079 1156 3087 Fragments arranged by size: 3087 2079 1156 ApoI r'AATT_y Cuts at: 1493 2254 2393 2401 2505 2589 2757 2942 3009 Size: 761 139 8 104 84 168 185 67 Cuts at: 3009 3093 3110 3177 3631 3857 4009 6118 1493 Size: 84 17 67 454 226 152 2109 1697 Fragments arranged by size: 2109 1697 761 454 226 185 168 152 139 104 84 84 67 67 17 8 AseI AT'TA_AT Cuts at: 160 1032 4876 160 Size: 872 3844 1606 Fragments arranged by size: 3844 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 AvaI C'yCGr_G Cuts at: 1870 1943 2172 3647 1870 Size: 73 229 1475 4545 Fragments arranged by size: 4545 1475 229 73 BamHI G'GATC_C Cuts at: 3652 4019 3652 Size: 367 5955 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3643 5098 618 Size: 325 217 627 1856 1455 1842 Fragments arranged by size: 1856 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3641 1391 Size: 2250 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2041 928 Size: 971 142 5209 Fragments arranged by size: 5209 971 142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4513 4547 1903 Size: 34 2576 34 3678 Fragments arranged by size: 3678 2576 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4214 5424 4214 Size: 1210 5112 BclI T'GATC_A Cuts at: 1548 2082 1548 Size: 534 5788 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4933 136 Size: 107 122 71 631 605 3261 1525 Fragments arranged by size: 3261 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6317 6317 Size: 6322 BlpI GC'TnA_GC Cuts at: 3739 3739 Size: 6322 Bme1580I G_kGCm'C Cuts at: 1790 2304 4383 5539 1790 Size: 514 2079 1156 2573 Fragments arranged by size: 2573 2079 1156 514 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6322 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2144 4053 5011 Size: 416 53 83 399 24 727 1909 958 Cuts at: 5011 442 Size: 1753 Fragments arranged by size: 1909 1753 958 727 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6322 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3548 3548 Size: 6322 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2787 2871 2955 3039 3123 3207 3291 Size: 875 673 84 84 84 84 84 84 Cuts at: 3291 3375 3459 3543 4982 1239 Size: 84 84 84 1439 2579 Fragments arranged by size: 2579 1439 875 673 84 84 84 84 84 84 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4447 5312 5610 4447 Size: 865 298 5159 Fragments arranged by size: 5159 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2169 2555 4985 882 Size: 617 670 386 2430 2219 Fragments arranged by size: 2430 2219 670 617 386 BsaAI yAC'GTr Cuts at: 493 1204 3619 4113 493 Size: 711 2415 494 2702 Fragments arranged by size: 2702 2415 711 494 BsaBI GATnn'nnATC Cuts at: 6316 6316 Size: 6322 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4129 4511 5146 275 Size: 53 83 186 936 2596 382 635 1451 Fragments arranged by size: 2596 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4755 5153 5427 5574 5985 4755 Size: 398 274 147 411 5092 Fragments arranged by size: 5092 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6097 Fragments arranged by size: 6097 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2720 3478 1186 Size: 1534 758 4030 Fragments arranged by size: 4030 1534 758 BseYI C'CCAG_C Cuts at: 5525 6021 5525 Size: 496 5826 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2814 1163 Size: 573 1078 4671 Fragments arranged by size: 4671 1078 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3772 4533 5561 1540 Size: 275 186 1771 761 1028 2301 Fragments arranged by size: 2301 1771 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2304 4383 4468 5539 2304 Size: 2079 85 1071 3087 Fragments arranged by size: 3087 2079 1071 85 BsmI GAATG_Cn' Cuts at: 3833 3926 3833 Size: 93 6229 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2304 3641 4383 4468 5539 1391 Size: 399 514 1337 742 85 1071 2174 Fragments arranged by size: 2174 1337 1071 742 514 399 85 BspEI T'CCGG_A Cuts at: 5985 5985 Size: 6322 BspHI T'CATG_A Cuts at: 4211 5941 4211 Size: 1730 4592 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3677 844 Size: 2833 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4209 1394 Size: 429 2386 3507 Fragments arranged by size: 3507 2386 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3570 4817 4991 65 Size: 1183 2322 1247 174 1396 Fragments arranged by size: 2322 1396 1247 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2643 3147 4966 5153 1700 Size: 110 833 504 1819 187 2869 Fragments arranged by size: 2869 1819 833 504 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6322 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6322 BssSI C'ACGA_G Cuts at: 4382 5394 4382 Size: 1012 5310 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6322 BstBI TT'CG_AA Cuts at: 2007 5126 2007 Size: 3119 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6322 BstYI r'GATC_y Cuts at: 1972 3652 4019 4404 4421 5873 5959 5971 6317 Size: 1680 367 385 17 1452 86 12 346 Cuts at: 6317 1972 Size: 1977 Fragments arranged by size: 1977 1680 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5770 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5583 Fragments arranged by size: 5583 683 56 BtsI GCAGTG_nn' Cuts at: 3838 4632 4652 3838 Size: 794 20 5508 Fragments arranged by size: 5508 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6322 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6287 DraI TTT'AAA Cuts at: 3061 3626 3061 Size: 565 5757 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5329 809 Size: 507 4013 1802 Fragments arranged by size: 4013 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2183 3769 4658 Size: 54 1425 50 275 18 353 1586 889 Cuts at: 4658 6287 8 Size: 1629 43 Fragments arranged by size: 1629 1586 1425 889 353 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3769 1537 Size: 275 1957 4090 Fragments arranged by size: 4090 1957 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2117 2616 4252 1353 Size: 764 499 1636 3423 Fragments arranged by size: 3423 1636 764 499 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4909 5283 5429 387 Size: 1064 138 3320 374 146 1280 Fragments arranged by size: 3320 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2787 2871 2955 3039 3123 3207 Size: 393 482 673 84 84 84 84 84 Cuts at: 3207 3291 3375 3459 3543 4385 4982 5769 6037 Size: 84 84 84 84 842 597 787 268 Cuts at: 6037 1239 Size: 1524 Fragments arranged by size: 1524 842 787 673 597 482 393 268 84 84 84 84 84 84 84 84 84 EcoRI G'AATT_C Cuts at: 2505 2589 2757 3009 3093 3177 3631 4009 2505 Size: 84 168 252 84 84 454 378 4818 Fragments arranged by size: 4818 454 378 252 168 84 84 84 FspI TGC'GCA Cuts at: 4828 4828 Size: 6322 HaeII r_GCGC'y Cuts at: 1678 5469 1678 Size: 3791 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2074 2106 4963 4995 5037 Size: 32 135 32 502 32 2857 32 42 Cuts at: 5037 5069 1373 Size: 32 2626 Fragments arranged by size: 2857 2626 502 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3666 3912 669 Size: 988 281 1728 246 3079 Fragments arranged by size: 3079 1728 988 281 246 HindIII A'AGCT_T Cuts at: 748 2718 2886 3054 3222 3306 3390 748 Size: 1970 168 168 168 84 84 3680 Fragments arranged by size: 3680 1970 168 168 168 84 84 HpaI GTT'AAC Cuts at: 3912 3912 Size: 6322 KpnI G_GTAC'C Cuts at: 3647 3647 Size: 6322 MfeI C'AATT_G Cuts at: 3921 3921 Size: 6322 MluI A'CGCG_T Cuts at: 6198 6198 Size: 6322 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5436 5620 1305 Size: 4131 184 2007 Fragments arranged by size: 4131 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2185 10 Size: 54 1425 343 353 4147 Fragments arranged by size: 4147 1425 353 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4280 4639 4798 518 Size: 648 3114 359 159 2042 Fragments arranged by size: 3114 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3738 4415 5563 5808 1606 Size: 2132 677 1148 245 2120 Fragments arranged by size: 2132 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5770 NdeI CA'TA_TG Cuts at: 387 387 Size: 6322 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6322 NotI GC'GGCC_GC Cuts at: 3769 3769 Size: 6322 NspI r_CATG'y Cuts at: 1132 1229 2479 3680 5225 1132 Size: 97 1250 1201 1545 2229 Fragments arranged by size: 2229 1545 1250 1201 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6322 PciI A'CATG_T Cuts at: 5221 5221 Size: 6322 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6322 PmeI GTTT'AAAC Cuts at: 3626 3626 Size: 6322 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4391 4423 5904 5936 4391 Size: 32 1481 32 4777 Fragments arranged by size: 4777 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6322 PsiI TTA'TAA Cuts at: 3892 3892 Size: 6322 PstI C_TGCA'G Cuts at: 830 3674 830 Size: 2844 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6322 SalI G'TCGA_C Cuts at: 1936 3664 1936 Size: 1728 4594 SbfI CC_TGCA'GG Cuts at: 3674 3674 Size: 6322 ScaI AGT'ACT Cuts at: 4570 6282 4570 Size: 1712 4610 SexAI A'CCwGG_T Cuts at: 2043 2043 Size: 6322 SfcI C'TryA_G Cuts at: 826 1045 2035 3670 4805 5486 5677 826 Size: 219 990 1635 1135 681 191 1471 Fragments arranged by size: 1635 1471 1135 990 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6322 SmaI CCC'GGG Cuts at: 1872 3649 1872 Size: 1777 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4426 5327 5589 820 Size: 197 926 2483 901 262 1553 Fragments arranged by size: 2483 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3619 4113 493 Size: 3126 494 2702 Fragments arranged by size: 3126 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6322 SphI G_CATG'C Cuts at: 1132 2479 3680 1132 Size: 1347 1201 3774 Fragments arranged by size: 3774 1347 1201 SspI AAT'ATT Cuts at: 5 52 2358 4246 5 Size: 47 2306 1888 2081 Fragments arranged by size: 2306 2081 1888 47 StuI AGG'CCT Cuts at: 1517 2972 1517 Size: 1455 4867 StyI C'CwwG_G Cuts at: 513 1065 2111 3761 513 Size: 552 1046 1650 3074 Fragments arranged by size: 3074 1650 1046 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4349 4366 4519 4704 5149 325 Size: 1255 2769 17 153 185 445 1498 Fragments arranged by size: 2769 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4568 6280 96 Size: 274 80 33 51 1443 2591 1712 138 Fragments arranged by size: 2591 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6282 6314 6282 Size: 32 6290 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6322 XbaI T'CTAG_A Cuts at: 3658 3658 Size: 6322 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6322 XmaI C'CCGG_G Cuts at: 1870 3647 1870 Size: 1777 4545 XmnI GAAnn'nnTTC Cuts at: 2441 3013 4451 2441 Size: 572 1438 4312 Fragments arranged by size: 4312 1438 572 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4130 276 Size: 53 83 186 936 2596 2468 Fragments arranged by size: 2596 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI