(Circular) (Six-base) MAPSORT of: pFN21ASDA1470.seq Check: 4325 from: 1 to: 6295 pFN21ASDA1470 6295 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3212 3212 Size: 6295 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4105 278 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3638 1937 Size: 1701 4594 Acc65I G'GTAC_C Cuts at: 3616 3616 Size: 6295 AclI AA'CG_TT Cuts at: 1563 4422 4795 1563 Size: 2859 373 3063 Fragments arranged by size: 3063 2859 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4358 5742 6010 1632 Size: 2726 1384 268 1917 Fragments arranged by size: 2726 1917 1384 268 AfeI AGC'GCT Cuts at: 2144 2144 Size: 6295 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6098 AflIII A'CryG_T Cuts at: 1203 5194 6171 1203 Size: 3991 977 1327 Fragments arranged by size: 3991 1327 977 AgeI A'CCGG_T Cuts at: 5126 5126 Size: 6295 AhdI GACnn_n'nnGTC Cuts at: 5024 5024 Size: 6295 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6295 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6295 AlwNI CAG_nnn'CTG Cuts at: 2747 2995 4038 5610 2747 Size: 248 1043 1572 3432 Fragments arranged by size: 3432 1572 1043 248 ApaI G_GGCC'C Cuts at: 2097 2209 2439 2097 Size: 112 230 5953 Fragments arranged by size: 5953 230 112 ApaLI G'TGCA_C Cuts at: 4352 5508 4352 Size: 1156 5139 ApoI r'AATT_y Cuts at: 1493 2425 2813 3315 3604 3830 3982 6091 1493 Size: 932 388 502 289 226 152 2109 1697 Fragments arranged by size: 2109 1697 932 502 388 289 226 152 AseI AT'TA_AT Cuts at: 160 1032 4849 160 Size: 872 3817 1606 Fragments arranged by size: 3817 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 AvaI C'yCGr_G Cuts at: 1870 1943 2068 2138 2285 2485 2910 3620 1870 Size: 73 125 70 147 200 425 710 4545 Fragments arranged by size: 4545 710 425 200 147 125 73 70 BamHI G'GATC_C Cuts at: 3625 3992 3625 Size: 367 5928 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2451 2859 3616 5071 618 Size: 325 217 627 664 408 757 1455 1842 Fragments arranged by size: 1842 1455 757 664 627 408 325 217 BanII G_rGCy'C Cuts at: 1391 2069 2097 2199 2209 2439 2492 2687 3614 Size: 678 28 102 10 230 53 195 927 Cuts at: 3614 1391 Size: 4072 Fragments arranged by size: 4072 927 678 230 195 102 53 28 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2981 3067 3426 928 Size: 971 1082 86 359 3797 Fragments arranged by size: 3797 1082 971 359 86 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4486 4520 1903 Size: 34 2549 34 3678 Fragments arranged by size: 3678 2549 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4187 5397 4187 Size: 1210 5085 BclI T'GATC_A Cuts at: 1548 1548 Size: 6295 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2118 2807 3108 Size: 107 122 71 631 605 446 689 301 Cuts at: 3108 4906 136 Size: 1798 1525 Fragments arranged by size: 1798 1525 689 631 605 446 301 122 107 71 BglII A'GATC_T Cuts at: 3186 6290 3186 Size: 3104 3191 BlpI GC'TnA_GC Cuts at: 3712 3712 Size: 6295 Bme1580I G_kGCm'C Cuts at: 1790 2097 2209 2439 2864 2917 4356 5512 1790 Size: 307 112 230 425 53 1439 1156 2573 Fragments arranged by size: 2573 1439 1156 425 307 230 112 53 BmgBI CAC'GTC Cuts at: 1318 3025 1318 Size: 1707 4588 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2371 3416 4026 Size: 416 53 83 399 24 954 1045 610 Cuts at: 4026 4984 442 Size: 958 1753 Fragments arranged by size: 1753 1045 958 954 610 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6295 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2083 2083 Size: 6295 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 3331 4955 1239 Size: 974 1118 1624 2579 Fragments arranged by size: 2579 1624 1118 974 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4420 5285 5583 4420 Size: 865 298 5132 Fragments arranged by size: 5132 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2488 4958 882 Size: 617 989 2470 2219 Fragments arranged by size: 2470 2219 989 617 BsaAI yAC'GTr Cuts at: 493 1204 3592 4086 493 Size: 711 2388 494 2702 Fragments arranged by size: 2702 2388 711 494 BsaBI GATnn'nnATC Cuts at: 6289 6289 Size: 6295 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4102 4484 5119 275 Size: 53 83 186 936 2569 382 635 1451 Fragments arranged by size: 2569 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4728 5126 5400 5547 5958 4728 Size: 398 274 147 411 5065 Fragments arranged by size: 5065 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2493 2523 2530 2560 3191 Size: 30 165 30 1090 30 7 30 631 Cuts at: 3191 3221 3491 3521 1178 Size: 30 270 30 3952 Fragments arranged by size: 3952 1090 631 270 165 30 30 30 30 30 30 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 2501 1186 Size: 886 429 4980 Fragments arranged by size: 4980 886 429 BseYI C'CCAG_C Cuts at: 2541 2566 2722 2753 2878 3106 3378 5498 5994 Size: 25 156 31 125 228 272 2120 496 Cuts at: 5994 2541 Size: 2842 Fragments arranged by size: 2842 2120 496 272 228 156 125 31 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2484 3314 1163 Size: 573 748 830 4144 Fragments arranged by size: 4144 830 748 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2237 2268 3745 4506 5534 1540 Size: 275 186 236 31 1477 761 1028 2301 Fragments arranged by size: 2301 1477 1028 761 275 236 186 31 BsiHKAI G_wGCw'C Cuts at: 2069 2199 2292 3371 4356 4441 5512 2069 Size: 130 93 1079 985 85 1071 2852 Fragments arranged by size: 2852 1079 1071 985 130 93 85 BsmI GAATG_Cn' Cuts at: 3806 3899 3806 Size: 93 6202 BsmBI CGTCTCn'nnnn_ Cuts at: 3015 3015 Size: 6295 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2097 2199 2209 2292 2439 2492 Size: 399 279 28 102 10 83 147 53 Cuts at: 2492 2687 2864 2917 3371 3614 4356 4441 5512 Size: 195 177 53 454 243 742 85 1071 Cuts at: 5512 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 454 399 279 243 195 177 147 102 85 83 53 53 28 10 BspEI T'CCGG_A Cuts at: 5958 5958 Size: 6295 BspHI T'CATG_A Cuts at: 4184 5914 4184 Size: 1730 4565 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3212 3475 3650 844 Size: 2368 263 175 3489 Fragments arranged by size: 3489 2368 263 175 BsrBI CCG'CTC Cuts at: 1394 1823 2134 4182 1394 Size: 429 311 2048 3507 Fragments arranged by size: 3507 2048 429 311 BsrDI GCAATG_nn' Cuts at: 65 1248 4790 4964 65 Size: 1183 3542 174 1396 Fragments arranged by size: 3542 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2236 3102 4939 5126 1700 Size: 110 426 866 1837 187 2869 Fragments arranged by size: 2869 1837 866 426 187 110 BsrGI T'GTAC_A Cuts at: 96 2772 96 Size: 2676 3619 BssHII G'CGCG_C Cuts at: 1925 2224 1925 Size: 299 5996 BssSI C'ACGA_G Cuts at: 2491 4355 5367 2491 Size: 1864 1012 3419 Fragments arranged by size: 3419 1864 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6295 BstBI TT'CG_AA Cuts at: 2007 5099 2007 Size: 3092 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6295 BstXI CCAn_nnnn'nTGG Cuts at: 2447 2447 Size: 6295 BstYI r'GATC_y Cuts at: 1972 3186 3625 3992 4377 4394 5846 5932 5944 Size: 1214 439 367 385 17 1452 86 12 Cuts at: 5944 6290 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1214 439 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2268 2520 3492 513 Size: 552 1203 252 972 3316 Fragments arranged by size: 3316 1203 972 552 252 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2890 487 Size: 683 56 1664 3892 Fragments arranged by size: 3892 1664 683 56 BtsI GCAGTG_nn' Cuts at: 2079 3297 3438 3811 4605 4625 2079 Size: 1218 141 373 794 20 3749 Fragments arranged by size: 3749 1218 794 373 141 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6295 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6260 DraI TTT'AAA Cuts at: 3599 3599 Size: 6295 DraIII CAC_nnn'GTG Cuts at: 2629 2629 Size: 6295 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5302 809 Size: 507 3986 1802 Fragments arranged by size: 3986 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2234 2265 2779 Size: 54 1425 50 275 18 404 31 514 Cuts at: 2779 2958 3100 3354 3742 4631 6260 8 Size: 179 142 254 388 889 1629 43 Fragments arranged by size: 1629 1425 889 514 404 388 275 254 179 142 54 50 43 31 18 EagI C'GGCC_G Cuts at: 1537 1812 2234 2265 3742 1537 Size: 275 422 31 1477 4090 Fragments arranged by size: 4090 1477 422 275 31 EarI CTCTTCn'nnn_ Cuts at: 1353 3332 4225 1353 Size: 1979 893 3423 Fragments arranged by size: 3423 1979 893 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2668 4882 5256 5402 387 Size: 1064 138 1079 2214 374 146 1280 Fragments arranged by size: 2214 1280 1079 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2067 2197 2067 Size: 130 6165 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 3331 4358 4955 5742 6010 1239 Size: 393 581 1118 1027 597 787 268 1524 Fragments arranged by size: 1524 1118 1027 787 597 581 393 268 EcoO109I rG'GnC_Cy Cuts at: 2205 2206 2435 2436 3298 2205 Size: 1 229 1 862 5202 Fragments arranged by size: 5202 862 229 1 1 EcoRI G'AATT_C Cuts at: 2813 3315 3604 3982 2813 Size: 502 289 378 5126 Fragments arranged by size: 5126 502 378 289 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2354 3091 2354 Size: 737 5558 FspI TGC'GCA Cuts at: 4801 4801 Size: 6295 HaeII r_GCGC'y Cuts at: 1678 2146 3039 5442 1678 Size: 468 893 2403 2531 Fragments arranged by size: 2531 2403 893 468 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2530 2562 2946 2978 4936 Size: 32 135 32 958 32 384 32 1958 Cuts at: 4936 4968 5010 5042 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1958 958 384 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3639 3885 669 Size: 988 281 1701 246 3079 Fragments arranged by size: 3079 1701 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6295 HpaI GTT'AAC Cuts at: 3885 3885 Size: 6295 KpnI G_GTAC'C Cuts at: 3620 3620 Size: 6295 MfeI C'AATT_G Cuts at: 3894 3894 Size: 6295 MluI A'CGCG_T Cuts at: 6171 6171 Size: 6295 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5409 5593 1305 Size: 4104 184 2007 Fragments arranged by size: 4104 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2960 10 Size: 54 1425 343 1128 3345 Fragments arranged by size: 3345 1425 1128 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2445 3350 4253 4612 4771 518 Size: 648 1279 905 903 359 159 2042 Fragments arranged by size: 2042 1279 905 903 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2160 2175 2566 2722 2878 2920 3378 3711 Size: 554 15 391 156 156 42 458 333 Cuts at: 3711 4388 5536 5781 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 554 458 391 333 245 156 156 42 15 NaeI GCC'GGC Cuts at: 2238 3104 2238 Size: 866 5429 NcoI C'CATG_G Cuts at: 513 1065 3492 513 Size: 552 2427 3316 Fragments arranged by size: 3316 2427 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6295 NgoMIV G'CCGG_C Cuts at: 2236 3102 2236 Size: 866 5429 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6295 NotI GC'GGCC_GC Cuts at: 3742 3742 Size: 6295 NspI r_CATG'y Cuts at: 1132 1229 2649 3653 5198 1132 Size: 97 1420 1004 1545 2229 Fragments arranged by size: 2229 1545 1420 1004 97 PasI CC'CwG_GG Cuts at: 1348 3167 1348 Size: 1819 4476 PciI A'CATG_T Cuts at: 5194 5194 Size: 6295 PflMI CCAn_nnn'nTGG Cuts at: 1108 2665 3049 3378 1108 Size: 1557 384 329 4025 Fragments arranged by size: 4025 1557 384 329 PfoI T'CCnGG_A Cuts at: 3054 3054 Size: 6295 PmeI GTTT'AAAC Cuts at: 3599 3599 Size: 6295 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4364 4396 5877 5909 4364 Size: 32 1481 32 4750 Fragments arranged by size: 4750 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3298 3298 Size: 6295 PshAI GACnn'nnGTC Cuts at: 1613 2480 1613 Size: 867 5428 PsiI TTA'TAA Cuts at: 3865 3865 Size: 6295 PspOMI G'GGCC_C Cuts at: 2093 2205 2435 2093 Size: 112 230 5953 Fragments arranged by size: 5953 230 112 PstI C_TGCA'G Cuts at: 830 2150 2644 3445 3647 830 Size: 1320 494 801 202 3478 Fragments arranged by size: 3478 1320 801 494 202 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6295 PvuII CAG'CTG Cuts at: 2566 2722 2878 3378 2566 Size: 156 156 500 5483 Fragments arranged by size: 5483 500 156 156 SacI G_AGCT'C Cuts at: 2069 2199 2069 Size: 130 6165 SalI G'TCGA_C Cuts at: 1936 3637 1936 Size: 1701 4594 SapI GCTCTTCn'nnn_ Cuts at: 3332 3332 Size: 6295 SbfI CC_TGCA'GG Cuts at: 3647 3647 Size: 6295 ScaI AGT'ACT Cuts at: 4543 6255 4543 Size: 1712 4583 SfcI C'TryA_G Cuts at: 826 1045 2146 2640 3441 3643 4778 5459 5650 Size: 219 1101 494 801 202 1135 681 191 Cuts at: 5650 826 Size: 1471 Fragments arranged by size: 1471 1135 1101 801 681 494 219 202 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3108 3108 Size: 6295 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6295 SmaI CCC'GGG Cuts at: 1872 2912 3622 1872 Size: 1040 710 4545 Fragments arranged by size: 4545 1040 710 SmlI C'TyrA_G Cuts at: 820 1017 1943 4399 5300 5562 820 Size: 197 926 2456 901 262 1553 Fragments arranged by size: 2456 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3592 4086 493 Size: 3099 494 2702 Fragments arranged by size: 3099 2702 494 SpeI A'CTAG_T Cuts at: 152 2941 152 Size: 2789 3506 SphI G_CATG'C Cuts at: 1132 3653 1132 Size: 2521 3774 SrfI GCCC'GGGC Cuts at: 2912 2912 Size: 6295 SspI AAT'ATT Cuts at: 5 52 4219 5 Size: 47 4167 2081 Fragments arranged by size: 4167 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6295 StyI C'CwwG_G Cuts at: 513 1065 2295 2664 2728 3492 3510 3734 513 Size: 552 1230 369 64 764 18 224 3074 Fragments arranged by size: 3074 1230 764 552 369 224 64 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2408 2439 2847 3218 4322 4339 4492 Size: 1255 828 31 408 371 1104 17 153 Cuts at: 4492 4677 5122 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1104 828 445 408 371 185 153 31 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2772 4541 6253 Size: 274 80 33 51 1443 795 1769 1712 Cuts at: 6253 96 Size: 138 Fragments arranged by size: 1769 1712 1443 795 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2493 2525 6255 6287 2493 Size: 32 3730 32 2501 Fragments arranged by size: 3730 2501 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6295 XbaI T'CTAG_A Cuts at: 3631 3631 Size: 6295 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3191 3191 Size: 6295 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6295 XmaI C'CCGG_G Cuts at: 1870 2910 3620 1870 Size: 1040 710 4545 Fragments arranged by size: 4545 1040 710 XmnI GAAnn'nnTTC Cuts at: 2943 4424 2943 Size: 1481 4814 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4103 276 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfoI SwaI