Restriction map for
pFN21ASDA1470
(Linear) (Six-base) MAPSORT of: KIAA1470.seq Check: 1232 from: 1 to: 1566
KIAA1470.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1189 1566
Size: 1189 377
AfeI AGC'GCT
Cuts at: 0 121 1566
Size: 121 1445
AlwNI CAG_nnn'CTG
Cuts at: 0 724 972 1566
Size: 724 248 594
Fragments arranged by size:
724 594 248
ApaI G_GGCC'C
Cuts at: 0 74 186 416 1566
Size: 74 112 230 1150
Fragments arranged by size:
1150 230 112 74
ApoI r'AATT_y
Cuts at: 0 402 790 1292 1566
Size: 402 388 502 274
Fragments arranged by size:
502 402 388 274
AvaI C'yCGr_G
Cuts at: 0 45 115 262 462 887 1566
Size: 45 70 147 200 425 679
Fragments arranged by size:
679 425 200 147 70 45
BanI G'GyrC_C
Cuts at: 0 428 836 1566
Size: 428 408 730
Fragments arranged by size:
730 428 408
BanII G_rGCy'C
Cuts at: 0 46 74 176 186 416 469 664 1566
Size: 46 28 102 10 230 53 195 902
Fragments arranged by size:
902 230 195 102 53 46 28 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 958 1044 1403 1566
Size: 958 86 359 163
Fragments arranged by size:
958 359 163 86
BglI GCCn_nnn'nGGC
Cuts at: 0 95 784 1085 1566
Size: 95 689 301 481
Fragments arranged by size:
689 481 301 95
BglII A'GATC_T
Cuts at: 0 1163 1566
Size: 1163 403
Bme1580I G_kGCm'C
Cuts at: 0 74 186 416 841 894 1566
Size: 74 112 230 425 53 672
Fragments arranged by size:
672 425 230 112 74 53
BmgBI CAC'GTC
Cuts at: 0 1002 1566
Size: 1002 564
BmrI ACTGGGnnnn_n'
Cuts at: 0 348 1393 1566
Size: 348 1045 173
Fragments arranged by size:
1045 348 173
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 60 1566
Size: 60 1506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1308 1566
Size: 190 1118 258
Fragments arranged by size:
1118 258 190
BsaI GGTCTCn'nnnn_
Cuts at: 0 465 1566
Size: 465 1101
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 470 500 507 537 1168 1198 1468 1498
Size: 470 30 7 30 631 30 270 30
Cuts at: 1498 1566
Size: 68
Fragments arranged by size:
631 470 270 68 30 30 30 30
7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 478 1566
Size: 49 429 1088
Fragments arranged by size:
1088 429 49
BseYI C'CCAG_C
Cuts at: 0 518 543 699 730 855 1083 1355 1566
Size: 518 25 156 31 125 228 272 211
Fragments arranged by size:
518 272 228 211 156 125 31 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 1291 1566
Size: 461 830 275
Fragments arranged by size:
830 461 275
BsiEI CG_ry'CG
Cuts at: 0 214 245 1566
Size: 214 31 1321
Fragments arranged by size:
1321 214 31
BsiHKAI G_wGCw'C
Cuts at: 0 46 176 269 1348 1566
Size: 46 130 93 1079 218
Fragments arranged by size:
1079 218 130 93 46
BsmBI CGTCTCn'nnnn_
Cuts at: 0 992 1566
Size: 992 574
Bsp1286I G_dGCh'C
Cuts at: 0 46 74 176 186 269 416 469 664
Size: 46 28 102 10 83 147 53 195
Cuts at: 664 841 894 1348 1566
Size: 177 53 454 218
Fragments arranged by size:
454 218 195 177 147 102 83 53
53 46 28 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1189 1452 1566
Size: 1189 263 114
Fragments arranged by size:
1189 263 114
BsrBI CCG'CTC
Cuts at: 0 111 1566
Size: 111 1455
BsrFI r'CCGG_y
Cuts at: 0 213 1079 1566
Size: 213 866 487
Fragments arranged by size:
866 487 213
BsrGI T'GTAC_A
Cuts at: 0 749 1566
Size: 749 817
BssHII G'CGCG_C
Cuts at: 0 201 1566
Size: 201 1365
BssSI C'ACGA_G
Cuts at: 0 468 1566
Size: 468 1098
BstXI CCAn_nnnn'nTGG
Cuts at: 0 424 1566
Size: 424 1142
BstYI r'GATC_y
Cuts at: 0 1163 1566
Size: 1163 403
BtgI C'CryG_G
Cuts at: 0 245 497 1469 1566
Size: 245 252 972 97
Fragments arranged by size:
972 252 245 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 867 1566
Size: 867 699
BtsI GCAGTG_nn'
Cuts at: 0 56 1274 1415 1566
Size: 56 1218 141 151
Fragments arranged by size:
1218 151 141 56
DraIII CAC_nnn'GTG
Cuts at: 0 606 1566
Size: 606 960
EaeI y'GGCC_r
Cuts at: 0 211 242 756 935 1077 1331 1566
Size: 211 31 514 179 142 254 235
Fragments arranged by size:
514 254 235 211 179 142 31
EagI C'GGCC_G
Cuts at: 0 211 242 1566
Size: 211 31 1324
Fragments arranged by size:
1324 211 31
EarI CTCTTCn'nnn_
Cuts at: 0 1309 1566
Size: 1309 257
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 645 1566
Size: 645 921
EcoICRI GAG'CTC
Cuts at: 0 44 174 1566
Size: 44 130 1392
Fragments arranged by size:
1392 130 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1308 1566
Size: 190 1118 258
Fragments arranged by size:
1118 258 190
EcoO109I rG'GnC_Cy
Cuts at: 0 182 183 412 413 1275 1566
Size: 182 1 229 1 862 291
Fragments arranged by size:
862 291 229 182 1 1
EcoRI G'AATT_C
Cuts at: 0 790 1292 1566
Size: 790 502 274
Fragments arranged by size:
790 502 274
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 331 1068 1566
Size: 331 737 498
Fragments arranged by size:
737 498 331
HaeII r_GCGC'y
Cuts at: 0 123 1016 1566
Size: 123 893 550
Fragments arranged by size:
893 550 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 507 539 923 955 1566
Size: 507 32 384 32 611
Fragments arranged by size:
611 507 384 32 32
MscI TGG'CCA
Cuts at: 0 937 1566
Size: 937 629
MslI CAynn'nnrTG
Cuts at: 0 422 1327 1566
Size: 422 905 239
Fragments arranged by size:
905 422 239
MspA1I CmG'CkG
Cuts at: 0 137 152 543 699 855 897 1355 1566
Size: 137 15 391 156 156 42 458 211
Fragments arranged by size:
458 391 211 156 156 137 42 15
NaeI GCC'GGC
Cuts at: 0 215 1081 1566
Size: 215 866 485
Fragments arranged by size:
866 485 215
NcoI C'CATG_G
Cuts at: 0 1469 1566
Size: 1469 97
NgoMIV G'CCGG_C
Cuts at: 0 213 1079 1566
Size: 213 866 487
Fragments arranged by size:
866 487 213
NspI r_CATG'y
Cuts at: 0 626 1566
Size: 626 940
PasI CC'CwG_GG
Cuts at: 0 1144 1566
Size: 1144 422
PflMI CCAn_nnn'nTGG
Cuts at: 0 642 1026 1355 1566
Size: 642 384 329 211
Fragments arranged by size:
642 384 329 211
PfoI T'CCnGG_A
Cuts at: 0 1031 1566
Size: 1031 535
PpuMI rG'GwC_Cy
Cuts at: 0 1275 1566
Size: 1275 291
PshAI GACnn'nnGTC
Cuts at: 0 457 1566
Size: 457 1109
PspOMI G'GGCC_C
Cuts at: 0 70 182 412 1566
Size: 70 112 230 1154
Fragments arranged by size:
1154 230 112 70
PstI C_TGCA'G
Cuts at: 0 127 621 1422 1566
Size: 127 494 801 144
Fragments arranged by size:
801 494 144 127
PvuII CAG'CTG
Cuts at: 0 543 699 855 1355 1566
Size: 543 156 156 500 211
Fragments arranged by size:
543 500 211 156 156
SacI G_AGCT'C
Cuts at: 0 46 176 1566
Size: 46 130 1390
Fragments arranged by size:
1390 130 46
SapI GCTCTTCn'nnn_
Cuts at: 0 1309 1566
Size: 1309 257
SfcI C'TryA_G
Cuts at: 0 123 617 1418 1566
Size: 123 494 801 148
Fragments arranged by size:
801 494 148 123
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1085 1566
Size: 1085 481
SmaI CCC'GGG
Cuts at: 0 889 1566
Size: 889 677
SpeI A'CTAG_T
Cuts at: 0 918 1566
Size: 918 648
SrfI GCCC'GGGC
Cuts at: 0 889 1566
Size: 889 677
StyI C'CwwG_G
Cuts at: 0 272 641 705 1469 1487 1566
Size: 272 369 64 764 18 79
Fragments arranged by size:
764 369 272 79 64 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 385 416 824 1195 1566
Size: 385 31 408 371 371
Fragments arranged by size:
408 385 371 371 31
TatI w'GTAC_w
Cuts at: 0 749 1566
Size: 749 817
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 470 502 1566
Size: 470 32 1064
Fragments arranged by size:
1064 470 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1168 1566
Size: 1168 398
XmaI C'CCGG_G
Cuts at: 0 887 1566
Size: 887 679
XmnI GAAnn'nnTTC
Cuts at: 0 920 1566
Size: 920 646
Enzymes that do cut:
AarI AfeI AlwNI ApaI ApoI AvaI BanI BanII
BbsI BglI BglII Bme1580I BmgBI BmrI BplI BpmI
BsaI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstXI
BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI
PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI
SapI SfcI SfiI SmaI SpeI SrfI StyI TaqII
TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BlpI BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaWI BsiWI BsmI BspEI BspHI BsrDI BstAPI BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoNI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NarI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI SphI
SspI StuI SwaI Tth111I XbaI XhoI ZraI