Restriction map for
pFN21ASDA1458
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BbrPI
BcgI BciVI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BssHII BssSI
BstAPI BstBI BstEII BstPI CelII
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco52I EcoO109I EcoO65I EcoRI EcoRV
FseI FspI HindIII KpnI KspI
MamI MluI MluNI MroI MscI
NdeI NotI NruI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspI StuI SwaI Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 1003 GTATAC GT^[AC][GT]AC
AlwNI 52 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1345 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1558 AGCGCT AGC^GCT
AspHI 1521 GAGCAC G[AT]GC[AT]^C
AspI 1232 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 30 GGACC G^G[AT]CC
BamHI 799 GGATCC G^GATCC
BanI 118 GGCGCC G^G[CT][GA]CC
BbeI 118 GGCGCC GGCGC^C
BbsI 1238 GTCTTC GAAGAC(2/6)
BpuAI 1238 GTCTTC GAAGAC(2/6)
BsiEI 1051 CGACCG CG[GA][CT]^CG
BsiHKAI 1521 GAGCAC G[AT]GC[AT]^C
Bsp1407I 831 TGTACA T^GTACA
BspMI 1575 ACCTGC ACCTGC(4/8)
BsrBI 99 CCGCTC CCGCTC(-3/-3)
BsrGI 831 TGTACA T^GTACA
Bst1107I 1003 GTATAC GTA^TAC
BstZ17I 1003 GTATAC GTA^TAC
Bsu36I 1345 CCTCAGG CC^T[ACGT]AGG
DsaI 1440 CCATGG C^C[GA][CT]GG
Eco47III 1558 AGCGCT AGC^GCT
Eco81I 1345 CCTCAGG CC^T[ACGT]AGG
EcoNI 1363 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 991 ATGCAT ATGCA^T
KasI 118 GGCGCC G^GCGCC
MfeI 794 CAATTG C^AATTG
MslI 1638 CATACCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 794 CAATTG C^AATTG
NarI 118 GGCGCC GG^CGCC
NcoI 1440 CCATGG C^CATGG
NheI 291 GCTAGC G^CTAGC
NsiI 991 ATGCAT ATGCA^T
PflFI 1232 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 88 CAGCTG CAG^CTG
SbfI 1576 CCTGCAGG CCTGCA^GG
SfoI 118 GGCGCC GGC^GCC
SmaI 235 CCCGGG CCC^GGG
SmlI 541 CTCAAG C^T[CT][GA]AG
Sse8387I 1576 CCTGCAGG CCTGCA^GG
SspBI 831 TGTACA T^GTACA
TaqI 1050 TCGA T^CGA
Tth111I 1232 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1050 TCGA T^CGA
XmaCI 235 CCCGGG C^CCGGG
XmaI 235 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 832 GTAC GT^AC
935 GTAC
BbvCI 210 CCTCAGC CCTCAGC(-5/-2)
90 GCTGAGG
BclI 522 TGATCA T^GATCA
945 TGATCA
BfaI 292 CTAG C^TAG
841 CTAG
BglI 330 GCCGCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
356 GCCTGTCAGGC
BsgI 112 GTGCAG GTGCAG(16/14)
453 GTGCAG
BsmBI 185 CGTCTC CGTCTC(1/5)
890 CGTCTC
BsrDI 1205 GCAATG GCAATG(2/0)
1591 GCAATG
BsrFI 121 GCCGGC [GA]^CCGG[CT]
154 GCCGGC
BstXI 442 CCATTAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1440 CCATGGCTCTGG
BstYI 799 GGATCC [GA]^GATC[CT]
1678 AGATCC
Cfr10I 121 GCCGGC [GA]^CCGG[CT]
154 GCCGGC
EarI 1409 CTCTTC CTCTTC(1/4)
265 GAAGAG
FbaI 522 TGATCA T^GATCA
945 TGATCA
HpaI 10 GTTAAC GTT^AAC
1015 GTTAAC
Ksp632I 1409 CTCTTC CTCTTC(1/4)
265 GAAGAG
MaeI 292 CTAG C^TAG
841 CTAG
MflI 799 GGATCC [GA]^GATC[CT]
1678 AGATCC
MspA1I 88 CAGCTG C[AC]G^C[GT]G
331 CCGCTG
NaeI 121 GCCGGC GCC^GGC
154 GCCGGC
NgoMIV 121 GCCGGC G^CCGGC
154 GCCGGC
PleI 1276 GACTC GAGTC(4/5)
1300 GACTC
RsaI 832 GTAC GT^AC
935 GTAC
SapI 265 GAAGAGC GCTCTTC(1/4)
1408 GCTCTTC
TfiI 790 GATTC G^A[AT]TC
837 GATTC
XhoII 799 GGATCC [GA]^GATC[CT]
1678 AGATCC
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
AlwI ApaI ApoI AvaI BanII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI Tru9I TseI Tsp45I
Tsp509I TspRI