(Circular) (Six-base) MAPSORT of: pFN21ASDA1458.seq Check: 8282 from: 1 to: 6472 pFN21ASDA1458 6472 bp With 182 enzymes: SgfI * February 10, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4282 278 Size: 53 83 186 936 2746 2468 Fragments arranged by size: 2746 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3027 3815 1937 Size: 1090 788 4594 Fragments arranged by size: 4594 1090 788 Acc65I G'GTAC_C Cuts at: 3793 3793 Size: 6472 AclI AA'CG_TT Cuts at: 1563 4599 4972 1563 Size: 3036 373 3063 Fragments arranged by size: 3063 3036 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2781 3360 4535 5919 6187 1632 Size: 1149 579 1175 1384 268 1917 Fragments arranged by size: 1917 1384 1175 1149 579 268 AfeI AGC'GCT Cuts at: 3583 3583 Size: 6472 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6275 AflIII A'CryG_T Cuts at: 1203 5371 6348 1203 Size: 4168 977 1327 Fragments arranged by size: 4168 1327 977 AgeI A'CCGG_T Cuts at: 5303 5303 Size: 6472 AhdI GACnn_n'nnGTC Cuts at: 5201 5201 Size: 6472 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6472 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2916 3683 3722 1184 Size: 1732 767 39 3934 Fragments arranged by size: 3934 1732 767 39 AlwNI CAG_nnn'CTG Cuts at: 2080 4215 5787 2080 Size: 2135 1572 2765 Fragments arranged by size: 2765 2135 1572 ApaI G_GGCC'C Cuts at: 2160 2259 2270 2160 Size: 99 11 6362 Fragments arranged by size: 6362 99 11 ApaLI G'TGCA_C Cuts at: 4529 5685 4529 Size: 1156 5316 ApoI r'AATT_y Cuts at: 1493 2575 2719 3100 3328 3398 3781 4007 4159 Size: 1082 144 381 228 70 383 226 152 Cuts at: 4159 6268 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1082 383 381 228 226 152 144 70 AseI AT'TA_AT Cuts at: 160 1032 5026 160 Size: 872 3994 1606 Fragments arranged by size: 3994 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 AvaI C'yCGr_G Cuts at: 1870 1943 2125 2214 2258 2269 3126 3797 1870 Size: 73 182 89 44 11 857 671 4545 Fragments arranged by size: 4545 857 671 182 89 73 44 11 BamHI G'GATC_C Cuts at: 2822 3802 4169 2822 Size: 980 367 5125 Fragments arranged by size: 5125 980 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2141 3793 5248 618 Size: 325 217 627 354 1652 1455 1842 Fragments arranged by size: 1842 1652 1455 627 354 325 217 BanII G_rGCy'C Cuts at: 1391 2160 2259 2270 3791 1391 Size: 769 99 11 1521 4072 Fragments arranged by size: 4072 1521 769 99 11 BbeI G_GCGC'C Cuts at: 2145 2145 Size: 6472 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3254 928 Size: 971 1355 4146 Fragments arranged by size: 4146 1355 971 BbvCI CC'TCA_GC Cuts at: 2114 2234 2114 Size: 120 6352 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4663 4697 1903 Size: 34 2726 34 3678 Fragments arranged by size: 3678 2726 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4364 5574 4364 Size: 1210 5262 BclI T'GATC_A Cuts at: 1548 2545 2968 1548 Size: 997 423 5052 Fragments arranged by size: 5052 997 423 BfrBI ATG'CAT Cuts at: 3016 3016 Size: 6472 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2359 2385 5083 Size: 107 122 71 631 605 687 26 2698 Cuts at: 5083 136 Size: 1525 Fragments arranged by size: 2698 1525 687 631 605 122 107 71 26 BglII A'GATC_T Cuts at: 6467 6467 Size: 6472 BlpI GC'TnA_GC Cuts at: 3889 3889 Size: 6472 Bme1580I G_kGCm'C Cuts at: 1790 2160 2259 2270 3646 4533 5689 1790 Size: 370 99 11 1376 887 1156 2573 Fragments arranged by size: 2573 1376 1156 887 370 99 11 BmgBI CAC'GTC Cuts at: 1318 2913 1318 Size: 1595 4877 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2672 3633 4203 Size: 416 53 83 399 24 1255 961 570 Cuts at: 4203 5161 442 Size: 958 1753 Fragments arranged by size: 1753 1255 961 958 570 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2318 1056 Size: 1262 5210 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2393 3375 5132 1239 Size: 875 279 982 1757 2579 Fragments arranged by size: 2579 1757 982 875 279 Bpu10I CC'TnA_GC Cuts at: 2114 2234 3084 2114 Size: 120 850 5502 Fragments arranged by size: 5502 850 120 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2549 4597 5462 5760 2549 Size: 2048 865 298 3261 Fragments arranged by size: 3261 2048 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5135 882 Size: 617 3636 2219 Fragments arranged by size: 3636 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3769 4263 493 Size: 711 2565 494 2702 Fragments arranged by size: 2702 2565 711 494 BsaBI GATnn'nnATC Cuts at: 6466 6466 Size: 6472 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2142 2207 2301 4279 Size: 53 83 186 936 609 65 94 1978 Cuts at: 4279 4661 5296 275 Size: 382 635 1451 Fragments arranged by size: 1978 1451 936 635 609 382 186 94 83 65 53 BsaWI w'CCGG_w Cuts at: 4905 5303 5577 5724 6135 4905 Size: 398 274 147 411 5242 Fragments arranged by size: 5242 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2021 2051 2291 2321 2366 Size: 30 165 30 618 30 240 30 45 Cuts at: 2366 2396 1178 Size: 30 5254 Fragments arranged by size: 5254 618 240 165 45 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2131 2185 2202 2408 2411 2414 2417 3508 Size: 945 54 17 206 3 3 3 1091 Cuts at: 3508 1186 Size: 4150 Fragments arranged by size: 4150 1091 945 206 54 17 3 3 3 BseYI C'CCAG_C Cuts at: 2388 3566 3653 5675 6171 2388 Size: 1178 87 2022 496 2689 Fragments arranged by size: 2689 2022 1178 496 87 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2156 2497 1163 Size: 573 420 341 5138 Fragments arranged by size: 5138 573 420 341 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3077 3922 4683 5711 1540 Size: 275 186 1076 845 761 1028 2301 Fragments arranged by size: 2301 1076 1028 845 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3548 4533 4618 5689 3548 Size: 985 85 1071 4331 Fragments arranged by size: 4331 1071 985 85 BsmI GAATG_Cn' Cuts at: 3983 4076 3983 Size: 93 6379 BsmBI CGTCTCn'nnnn_ Cuts at: 2214 2919 2214 Size: 705 5767 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2259 2270 3548 3646 3791 4533 Size: 399 370 99 11 1278 98 145 742 Cuts at: 4533 4618 5689 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1278 1071 742 399 370 145 99 98 85 11 BspEI T'CCGG_A Cuts at: 6135 6135 Size: 6472 BspHI T'CATG_A Cuts at: 4361 6091 4361 Size: 1730 4742 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3607 3827 844 Size: 2763 220 3489 Fragments arranged by size: 3489 2763 220 BsrBI CCG'CTC Cuts at: 1394 1823 2124 4359 1394 Size: 429 301 2235 3507 Fragments arranged by size: 3507 2235 429 301 BsrDI GCAATG_nn' Cuts at: 65 1248 3235 3621 4967 5141 65 Size: 1183 1987 386 1346 174 1396 Fragments arranged by size: 1987 1396 1346 1183 386 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2144 2177 5116 5303 1700 Size: 110 334 33 2939 187 2869 Fragments arranged by size: 2939 2869 334 187 110 33 BsrGI T'GTAC_A Cuts at: 96 2854 96 Size: 2758 3714 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6472 BssSI C'ACGA_G Cuts at: 4532 5544 4532 Size: 1012 5460 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6472 BstBI TT'CG_AA Cuts at: 2007 5276 2007 Size: 3269 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6472 BstXI CCAn_nnnn'nTGG Cuts at: 2472 3470 2472 Size: 998 5474 BstYI r'GATC_y Cuts at: 1972 2822 3701 3802 4169 4554 4571 6023 6109 Size: 850 879 101 367 385 17 1452 86 Cuts at: 6109 6121 6467 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 879 850 385 367 346 101 86 17 12 BstZ17I GTA'TAC Cuts at: 3028 3028 Size: 6472 Bsu36I CC'TnA_GG Cuts at: 3369 3369 Size: 6472 BtgI C'CryG_G Cuts at: 513 1065 2022 3463 513 Size: 552 957 1441 3522 Fragments arranged by size: 3522 1441 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5733 Fragments arranged by size: 5733 683 56 BtsI GCAGTG_nn' Cuts at: 2656 3988 4782 4802 2656 Size: 1332 794 20 4326 Fragments arranged by size: 4326 1332 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6472 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6437 DraI TTT'AAA Cuts at: 3776 3776 Size: 6472 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5479 809 Size: 507 4163 1802 Fragments arranged by size: 4163 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3919 4808 6437 Size: 54 1425 50 275 18 2089 889 1629 Cuts at: 6437 8 Size: 43 Fragments arranged by size: 2089 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3919 1537 Size: 275 2107 4090 Fragments arranged by size: 4090 2107 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2283 3438 4402 1353 Size: 930 1155 964 3423 Fragments arranged by size: 3423 1155 964 930 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2290 5059 5433 5579 387 Size: 1064 138 701 2769 374 146 1280 Fragments arranged by size: 2769 1280 1064 701 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2393 2781 3360 3375 4535 5132 Size: 393 482 279 388 579 15 1160 597 Cuts at: 5132 5919 6187 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1160 787 597 579 482 393 388 279 268 15 EcoNI CCTnn'n_nnAGG Cuts at: 3390 3390 Size: 6472 EcoRI G'AATT_C Cuts at: 3781 4159 3781 Size: 378 6094 FspI TGC'GCA Cuts at: 4978 4978 Size: 6472 HaeII r_GCGC'y Cuts at: 1678 2145 2241 2380 3585 5619 1678 Size: 467 96 139 1205 2034 2531 Fragments arranged by size: 2531 2034 1205 467 139 96 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 2291 2323 2366 Size: 32 135 32 449 32 238 32 43 Cuts at: 2366 2398 5113 5145 5187 5219 1373 Size: 32 2715 32 42 32 2626 Fragments arranged by size: 2715 2626 449 238 135 43 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2035 3040 3298 3816 4062 669 Size: 988 281 97 1005 258 518 246 3079 Fragments arranged by size: 3079 1005 988 518 281 258 246 97 HindIII A'AGCT_T Cuts at: 748 748 Size: 6472 HpaI GTT'AAC Cuts at: 2035 3040 4062 2035 Size: 1005 1022 4445 Fragments arranged by size: 4445 1022 1005 KasI G'GCGC_C Cuts at: 2141 2141 Size: 6472 KpnI G_GTAC'C Cuts at: 3797 3797 Size: 6472 MfeI C'AATT_G Cuts at: 2817 4071 2817 Size: 1254 5218 MluI A'CGCG_T Cuts at: 6348 6348 Size: 6472 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2064 3728 5586 5770 1305 Size: 759 1664 1858 184 2007 Fragments arranged by size: 2007 1858 1664 759 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4650 Fragments arranged by size: 4650 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3665 4430 4789 4948 518 Size: 648 2499 765 359 159 2042 Fragments arranged by size: 2499 2042 765 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2113 2356 3888 4565 5713 5958 1606 Size: 507 243 1532 677 1148 245 2120 Fragments arranged by size: 2120 1532 1148 677 507 245 243 NaeI GCC'GGC Cuts at: 2146 2179 2146 Size: 33 6439 NarI GG'CG_CC Cuts at: 2142 2142 Size: 6472 NcoI C'CATG_G Cuts at: 513 1065 2022 3463 513 Size: 552 957 1441 3522 Fragments arranged by size: 3522 1441 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6472 NgoMIV G'CCGG_C Cuts at: 2144 2177 2144 Size: 33 6439 NheI G'CTAG_C Cuts at: 1052 2314 1052 Size: 1262 5210 NotI GC'GGCC_GC Cuts at: 3919 3919 Size: 6472 NsiI A_TGCA'T Cuts at: 3018 3018 Size: 6472 NspI r_CATG'y Cuts at: 1132 1229 3830 5375 1132 Size: 97 2601 1545 2229 Fragments arranged by size: 2601 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6472 PciI A'CATG_T Cuts at: 5371 5371 Size: 6472 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6472 PfoI T'CCnGG_A Cuts at: 2713 2713 Size: 6472 PmeI GTTT'AAAC Cuts at: 3776 3776 Size: 6472 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4541 4573 6054 6086 4541 Size: 32 1481 32 4927 Fragments arranged by size: 4927 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6472 PsiI TTA'TAA Cuts at: 2832 4042 2832 Size: 1210 5262 PspOMI G'GGCC_C Cuts at: 2156 2255 2266 2156 Size: 99 11 6362 Fragments arranged by size: 6362 99 11 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3271 3303 3271 Size: 32 6440 PstI C_TGCA'G Cuts at: 830 2076 3415 3604 3824 830 Size: 1246 1339 189 220 3478 Fragments arranged by size: 3478 1339 1246 220 189 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6472 PvuII CAG'CTG Cuts at: 2113 2113 Size: 6472 SalI G'TCGA_C Cuts at: 1936 3814 1936 Size: 1878 4594 SapI GCTCTTCn'nnn_ Cuts at: 2283 3438 2283 Size: 1155 5317 SbfI CC_TGCA'GG Cuts at: 3604 3824 3604 Size: 220 6252 ScaI AGT'ACT Cuts at: 4720 6432 4720 Size: 1712 4760 SfcI C'TryA_G Cuts at: 826 1045 2072 2691 2775 3411 3600 3820 4955 Size: 219 1027 619 84 636 189 220 1135 Cuts at: 4955 5636 5827 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1027 681 636 619 220 219 191 189 84 SfoI GGC'GCC Cuts at: 2143 2143 Size: 6472 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6472 SmaI CCC'GGG Cuts at: 1872 2260 3799 1872 Size: 388 1539 4545 Fragments arranged by size: 4545 1539 388 SmlI C'TyrA_G Cuts at: 820 1017 1943 2564 4576 5477 5739 820 Size: 197 926 621 2012 901 262 1553 Fragments arranged by size: 2012 1553 926 901 621 262 197 SnaBI TAC'GTA Cuts at: 493 3769 4263 493 Size: 3276 494 2702 Fragments arranged by size: 3276 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6472 SphI G_CATG'C Cuts at: 1132 3830 1132 Size: 2698 3774 SspI AAT'ATT Cuts at: 5 52 4396 5 Size: 47 4344 2081 Fragments arranged by size: 4344 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6472 StyI C'CwwG_G Cuts at: 513 1065 2022 2152 2226 3463 3911 513 Size: 552 957 130 74 1237 448 3074 Fragments arranged by size: 3074 1237 957 552 448 130 74 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3109 4499 4516 4669 4854 5299 325 Size: 1255 1529 1390 17 153 185 445 1498 Fragments arranged by size: 1529 1498 1390 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2854 4718 6430 Size: 274 80 33 51 1443 877 1864 1712 Cuts at: 6430 96 Size: 138 Fragments arranged by size: 1864 1712 1443 877 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6432 6464 6432 Size: 32 6440 Tth111I GACn'n_nGTC Cuts at: 1316 3258 1316 Size: 1942 4530 XbaI T'CTAG_A Cuts at: 3808 3808 Size: 6472 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6472 XmaI C'CCGG_G Cuts at: 1870 2258 3797 1870 Size: 388 1539 4545 Fragments arranged by size: 4545 1539 388 XmnI GAAnn'nnTTC Cuts at: 4601 4601 Size: 6472 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4280 276 Size: 53 83 186 936 2746 2468 Fragments arranged by size: 2746 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BplI BsiWI DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI PacI PmlI PpuMI PspXI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI