Restriction map for
pFN21ASDA1458
(Linear) (Six-base) MAPSORT of: KIAA1458.seq Check: 2919 from: 1 to: 1743
KIAA1458.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AccI GT'mk_AC
Cuts at: 0 1004 1743
Size: 1004 739
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 1337 1743
Size: 758 579 406
Fragments arranged by size:
758 579 406
AfeI AGC'GCT
Cuts at: 0 1560 1743
Size: 1560 183
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 893 1660 1699 1743
Size: 893 767 39 44
Fragments arranged by size:
893 767 44 39
AlwNI CAG_nnn'CTG
Cuts at: 0 57 1743
Size: 57 1686
ApaI G_GGCC'C
Cuts at: 0 137 236 247 1743
Size: 137 99 11 1496
Fragments arranged by size:
1496 137 99 11
ApoI r'AATT_y
Cuts at: 0 552 696 1077 1305 1375 1743
Size: 552 144 381 228 70 368
Fragments arranged by size:
552 381 368 228 144 70
AvaI C'yCGr_G
Cuts at: 0 102 191 235 246 1103 1743
Size: 102 89 44 11 857 640
Fragments arranged by size:
857 640 102 89 44 11
BamHI G'GATC_C
Cuts at: 0 799 1743
Size: 799 944
BanI G'GyrC_C
Cuts at: 0 118 1743
Size: 118 1625
BanII G_rGCy'C
Cuts at: 0 137 236 247 1743
Size: 137 99 11 1496
Fragments arranged by size:
1496 137 99 11
BbeI G_GCGC'C
Cuts at: 0 122 1743
Size: 122 1621
BbsI GAAGACnn'nnnn_
Cuts at: 0 1231 1743
Size: 1231 512
BbvCI CC'TCA_GC
Cuts at: 0 91 211 1743
Size: 91 120 1532
Fragments arranged by size:
1532 120 91
BclI T'GATC_A
Cuts at: 0 522 945 1743
Size: 522 423 798
Fragments arranged by size:
798 522 423
BfrBI ATG'CAT
Cuts at: 0 993 1743
Size: 993 750
BglI GCCn_nnn'nGGC
Cuts at: 0 336 362 1743
Size: 336 26 1381
Fragments arranged by size:
1381 336 26
Bme1580I G_kGCm'C
Cuts at: 0 137 236 247 1623 1743
Size: 137 99 11 1376 120
Fragments arranged by size:
1376 137 120 99 11
BmgBI CAC'GTC
Cuts at: 0 890 1743
Size: 890 853
BmrI ACTGGGnnnn_n'
Cuts at: 0 649 1610 1743
Size: 649 961 133
Fragments arranged by size:
961 649 133
BmtI G_CTAG'C
Cuts at: 0 295 1743
Size: 295 1448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 370 1352 1743
Size: 91 279 982 391
Fragments arranged by size:
982 391 279 91
Bpu10I CC'TnA_GC
Cuts at: 0 91 211 1061 1743
Size: 91 120 850 682
Fragments arranged by size:
850 682 120 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 1743
Size: 526 1217
BsaHI Gr'CG_yC
Cuts at: 0 119 184 278 1743
Size: 119 65 94 1465
Fragments arranged by size:
1465 119 94 65
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 268 298 343 373 1743
Size: 28 240 30 45 30 1370
Fragments arranged by size:
1370 240 45 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 108 162 179 385 388 391 394 1485
Size: 108 54 17 206 3 3 3 1091
Cuts at: 1485 1743
Size: 258
Fragments arranged by size:
1091 258 206 108 54 17 3 3
3
BseYI C'CCAG_C
Cuts at: 0 365 1543 1630 1743
Size: 365 1178 87 113
Fragments arranged by size:
1178 365 113 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 474 1743
Size: 133 341 1269
Fragments arranged by size:
1269 341 133
BsiEI CG_ry'CG
Cuts at: 0 1054 1743
Size: 1054 689
BsiHKAI G_wGCw'C
Cuts at: 0 1525 1743
Size: 1525 218
BsmBI CGTCTCn'nnnn_
Cuts at: 0 191 896 1743
Size: 191 705 847
Fragments arranged by size:
847 705 191
Bsp1286I G_dGCh'C
Cuts at: 0 137 236 247 1525 1623 1743
Size: 137 99 11 1278 98 120
Fragments arranged by size:
1278 137 120 99 98 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1584 1743
Size: 1584 159
BsrBI CCG'CTC
Cuts at: 0 101 1743
Size: 101 1642
BsrDI GCAATG_nn'
Cuts at: 0 1212 1598 1743
Size: 1212 386 145
Fragments arranged by size:
1212 386 145
BsrFI r'CCGG_y
Cuts at: 0 121 154 1743
Size: 121 33 1589
Fragments arranged by size:
1589 121 33
BsrGI T'GTAC_A
Cuts at: 0 831 1743
Size: 831 912
BstXI CCAn_nnnn'nTGG
Cuts at: 0 449 1447 1743
Size: 449 998 296
Fragments arranged by size:
998 449 296
BstYI r'GATC_y
Cuts at: 0 799 1678 1743
Size: 799 879 65
Fragments arranged by size:
879 799 65
BstZ17I GTA'TAC
Cuts at: 0 1005 1743
Size: 1005 738
Bsu36I CC'TnA_GG
Cuts at: 0 1346 1743
Size: 1346 397
BtgI C'CryG_G
Cuts at: 0 1440 1743
Size: 1440 303
BtsI GCAGTG_nn'
Cuts at: 0 633 1743
Size: 633 1110
EarI CTCTTCn'nnn_
Cuts at: 0 260 1415 1743
Size: 260 1155 328
Fragments arranged by size:
1155 328 260
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 267 1743
Size: 267 1476
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 370 758 1337 1352 1743
Size: 91 279 388 579 15 391
Fragments arranged by size:
579 391 388 279 91 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1367 1743
Size: 1367 376
HaeII r_GCGC'y
Cuts at: 0 122 218 357 1562 1743
Size: 122 96 139 1205 181
Fragments arranged by size:
1205 181 139 122 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 268 300 343 375 1743
Size: 30 238 32 43 32 1368
Fragments arranged by size:
1368 238 43 32 32 30
HincII GTy'rAC
Cuts at: 0 12 1017 1275 1743
Size: 12 1005 258 468
Fragments arranged by size:
1005 468 258 12
HpaI GTT'AAC
Cuts at: 0 12 1017 1743
Size: 12 1005 726
Fragments arranged by size:
1005 726 12
KasI G'GCGC_C
Cuts at: 0 118 1743
Size: 118 1625
MfeI C'AATT_G
Cuts at: 0 794 1743
Size: 794 949
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 1705 1743
Size: 41 1664 38
Fragments arranged by size:
1664 41 38
MslI CAynn'nnrTG
Cuts at: 0 1642 1743
Size: 1642 101
MspA1I CmG'CkG
Cuts at: 0 90 333 1743
Size: 90 243 1410
Fragments arranged by size:
1410 243 90
NaeI GCC'GGC
Cuts at: 0 123 156 1743
Size: 123 33 1587
Fragments arranged by size:
1587 123 33
NarI GG'CG_CC
Cuts at: 0 119 1743
Size: 119 1624
NcoI C'CATG_G
Cuts at: 0 1440 1743
Size: 1440 303
NgoMIV G'CCGG_C
Cuts at: 0 121 154 1743
Size: 121 33 1589
Fragments arranged by size:
1589 121 33
NheI G'CTAG_C
Cuts at: 0 291 1743
Size: 291 1452
NsiI A_TGCA'T
Cuts at: 0 995 1743
Size: 995 748
PfoI T'CCnGG_A
Cuts at: 0 690 1743
Size: 690 1053
PsiI TTA'TAA
Cuts at: 0 809 1743
Size: 809 934
PspOMI G'GGCC_C
Cuts at: 0 133 232 243 1743
Size: 133 99 11 1500
Fragments arranged by size:
1500 133 99 11
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1248 1280 1743
Size: 1248 32 463
Fragments arranged by size:
1248 463 32
PstI C_TGCA'G
Cuts at: 0 53 1392 1581 1743
Size: 53 1339 189 162
Fragments arranged by size:
1339 189 162 53
PvuII CAG'CTG
Cuts at: 0 90 1743
Size: 90 1653
SapI GCTCTTCn'nnn_
Cuts at: 0 260 1415 1743
Size: 260 1155 328
Fragments arranged by size:
1155 328 260
SbfI CC_TGCA'GG
Cuts at: 0 1581 1743
Size: 1581 162
SfcI C'TryA_G
Cuts at: 0 49 668 752 1388 1577 1743
Size: 49 619 84 636 189 166
Fragments arranged by size:
636 619 189 166 84 49
SfoI GGC'GCC
Cuts at: 0 120 1743
Size: 120 1623
SmaI CCC'GGG
Cuts at: 0 237 1743
Size: 237 1506
SmlI C'TyrA_G
Cuts at: 0 541 1743
Size: 541 1202
StyI C'CwwG_G
Cuts at: 0 129 203 1440 1743
Size: 129 74 1237 303
Fragments arranged by size:
1237 303 129 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1086 1743
Size: 1086 657
TatI w'GTAC_w
Cuts at: 0 831 1743
Size: 831 912
Tth111I GACn'n_nGTC
Cuts at: 0 1235 1743
Size: 1235 508
XmaI C'CCGG_G
Cuts at: 0 235 1743
Size: 235 1508
Enzymes that do cut:
AccI AcuI AfeI AlfI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BclI BfrBI
BglI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstXI BstYI
BstZ17I Bsu36I BtgI BtsI EarI EciI Eco57MI EcoNI
HaeII Hin4I HincII HpaI KasI MfeI MmeI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI PfoI
PsiI PspOMI PsrI PstI PvuII SapI SbfI SfcI
SfoI SmaI SmlI StyI TaqII TatI Tth111I XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BcgI BciVI BglII BlpI BplI BsaI BsaAI BsaBI
BsaWI BsiWI BsmI BspEI BspHI BssHII BssSI BstAPI
BstBI BstEII BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EcoICRI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HindIII KpnI MluI MscI NdeI NotI
NruI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PspXI PvuI RsrII SacI SacII
SalI SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI XbaI
XcmI XhoI XmnI ZraI