(Circular) (Six-base) MAPSORT of: pFN21ASDA1456.seq Check: 462 from: 1 to: 5830 pFN21ASDA1456 5830 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3640 278 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3173 1937 Size: 1236 4594 Acc65I G'GTAC_C Cuts at: 3151 3151 Size: 5830 AclI AA'CG_TT Cuts at: 1563 3957 4330 1563 Size: 2394 373 3063 Fragments arranged by size: 3063 2394 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3893 5277 5545 1632 Size: 2261 1384 268 1917 Fragments arranged by size: 2261 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5633 AflIII A'CryG_T Cuts at: 1203 4729 5706 1203 Size: 3526 977 1327 Fragments arranged by size: 3526 1327 977 AgeI A'CCGG_T Cuts at: 4661 4661 Size: 5830 AhdI GACnn_n'nnGTC Cuts at: 4559 4559 Size: 5830 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5830 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5830 AlwNI CAG_nnn'CTG Cuts at: 3573 5145 3573 Size: 1572 4258 ApaI G_GGCC'C Cuts at: 2251 2251 Size: 5830 ApaLI G'TGCA_C Cuts at: 3887 5043 3887 Size: 1156 4674 ApoI r'AATT_y Cuts at: 1493 2803 3139 3365 3517 5626 1493 Size: 1310 336 226 152 2109 1697 Fragments arranged by size: 2109 1697 1310 336 226 152 AseI AT'TA_AT Cuts at: 160 1032 4384 160 Size: 872 3352 1606 Fragments arranged by size: 3352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 AvaI C'yCGr_G Cuts at: 1870 1943 3155 1870 Size: 73 1212 4545 Fragments arranged by size: 4545 1212 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3076 3109 3076 Size: 33 5797 BamHI G'GATC_C Cuts at: 3160 3527 3160 Size: 367 5463 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3151 4606 618 Size: 325 217 627 1364 1455 1842 Fragments arranged by size: 1842 1455 1364 627 325 217 BanII G_rGCy'C Cuts at: 1391 2251 3021 3054 3149 1391 Size: 860 770 33 95 4072 Fragments arranged by size: 4072 860 770 95 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4859 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4021 4055 1903 Size: 34 2084 34 3678 Fragments arranged by size: 3678 2084 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2300 3068 3722 4932 2300 Size: 768 654 1210 3198 Fragments arranged by size: 3198 1210 768 654 BclI T'GATC_A Cuts at: 1548 2644 3076 1548 Size: 1096 432 4302 Fragments arranged by size: 4302 1096 432 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4441 136 Size: 107 122 71 631 605 2769 1525 Fragments arranged by size: 2769 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2522 5825 2522 Size: 3303 2527 BlpI GC'TnA_GC Cuts at: 3247 3247 Size: 5830 Bme1580I G_kGCm'C Cuts at: 1790 2251 3891 5047 1790 Size: 461 1640 1156 2573 Fragments arranged by size: 2573 1640 1156 461 BmgBI CAC'GTC Cuts at: 1318 2227 1318 Size: 909 4921 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3561 4519 442 Size: 416 53 83 399 24 2144 958 1753 Fragments arranged by size: 2144 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2503 4490 1239 Size: 1264 1987 2579 Fragments arranged by size: 2579 1987 1264 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3017 3955 4820 5118 3017 Size: 938 865 298 3729 Fragments arranged by size: 3729 938 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2760 4493 882 Size: 617 1261 1733 2219 Fragments arranged by size: 2219 1733 1261 617 BsaAI yAC'GTr Cuts at: 493 1204 3127 3621 493 Size: 711 1923 494 2702 Fragments arranged by size: 2702 1923 711 494 BsaBI GATnn'nnATC Cuts at: 2865 5824 2865 Size: 2959 2871 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3637 4019 4654 275 Size: 53 83 186 936 2104 382 635 1451 Fragments arranged by size: 2104 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4263 4661 4935 5082 5493 4263 Size: 398 274 147 411 4600 Fragments arranged by size: 4600 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3029 3059 1178 Size: 30 165 30 1626 30 3949 Fragments arranged by size: 3949 1626 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2800 1186 Size: 1614 4216 BseYI C'CCAG_C Cuts at: 2259 5033 5529 2259 Size: 2774 496 2560 Fragments arranged by size: 2774 2560 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5257 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3280 4041 5069 1540 Size: 275 186 1279 761 1028 2301 Fragments arranged by size: 2301 1279 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2740 3021 3054 3891 3976 5047 2740 Size: 281 33 837 85 1071 3523 Fragments arranged by size: 3523 1071 837 281 85 33 BsmI GAATG_Cn' Cuts at: 3341 3434 3341 Size: 93 5737 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2251 2740 3021 3054 3149 3891 3976 Size: 399 461 489 281 33 95 742 85 Cuts at: 3976 5047 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 489 461 399 281 95 85 33 BspEI T'CCGG_A Cuts at: 5493 5493 Size: 5830 BspHI T'CATG_A Cuts at: 3719 5449 3719 Size: 1730 4100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2425 3185 844 Size: 1581 760 3489 Fragments arranged by size: 3489 1581 760 BsrBI CCG'CTC Cuts at: 1394 1823 3717 1394 Size: 429 1894 3507 Fragments arranged by size: 3507 1894 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2196 3096 4325 4499 65 Size: 1183 948 900 1229 174 1396 Fragments arranged by size: 1396 1229 1183 948 900 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4474 4661 1700 Size: 110 2664 187 2869 Fragments arranged by size: 2869 2664 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5830 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5830 BssSI C'ACGA_G Cuts at: 3890 4902 3890 Size: 1012 4818 BstAPI GCAn_nnn'nTGC Cuts at: 794 2227 794 Size: 1433 4397 BstBI TT'CG_AA Cuts at: 2007 4634 2007 Size: 2627 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5830 BstXI CCAn_nnnn'nTGG Cuts at: 2527 2527 Size: 5830 BstYI r'GATC_y Cuts at: 1972 2522 2950 3160 3527 3912 3929 5381 5467 Size: 550 428 210 367 385 17 1452 86 Cuts at: 5467 5479 5825 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 550 428 385 367 346 210 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2237 513 Size: 552 957 215 4106 Fragments arranged by size: 4106 957 552 215 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5091 Fragments arranged by size: 5091 683 56 BtsI GCAGTG_nn' Cuts at: 2306 3346 4140 4160 2306 Size: 1040 794 20 3976 Fragments arranged by size: 3976 1040 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5795 DraI TTT'AAA Cuts at: 2371 2760 3134 2371 Size: 389 374 5067 Fragments arranged by size: 5067 389 374 DraIII CAC_nnn'GTG Cuts at: 2404 2404 Size: 5830 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2631 4837 809 Size: 507 1315 2206 1802 Fragments arranged by size: 2206 1802 1315 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 3277 4166 Size: 54 1425 50 275 18 312 1135 889 Cuts at: 4166 5795 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1135 889 312 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3277 1537 Size: 275 1465 4090 Fragments arranged by size: 4090 1465 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2270 2288 3760 1353 Size: 917 18 1472 3423 Fragments arranged by size: 3423 1472 917 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4417 4791 4937 387 Size: 1064 138 2828 374 146 1280 Fragments arranged by size: 2828 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3019 3052 3019 Size: 33 5797 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2503 3893 4490 5277 5545 1239 Size: 393 871 1390 597 787 268 1524 Fragments arranged by size: 1524 1390 871 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2247 2248 2247 Size: 1 5829 EcoRI G'AATT_C Cuts at: 3139 3517 3139 Size: 378 5452 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2246 2246 Size: 5830 FspI TGC'GCA Cuts at: 4336 4336 Size: 5830 HaeII r_GCGC'y Cuts at: 1678 4977 1678 Size: 3299 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2924 2956 3029 3061 4471 Size: 32 135 32 1352 32 73 32 1410 Cuts at: 4471 4503 4545 4577 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1410 1352 135 73 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3174 3420 669 Size: 988 281 1236 246 3079 Fragments arranged by size: 3079 1236 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5830 HpaI GTT'AAC Cuts at: 3420 3420 Size: 5830 KpnI G_GTAC'C Cuts at: 3155 3155 Size: 5830 MfeI C'AATT_G Cuts at: 3429 3429 Size: 5830 MluI A'CGCG_T Cuts at: 5706 5706 Size: 5830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4944 5128 1305 Size: 3639 184 2007 Fragments arranged by size: 3639 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2144 10 Size: 54 1425 343 312 3696 Fragments arranged by size: 3696 1425 343 312 54 MslI CAynn'nnrTG Cuts at: 518 1166 2760 3788 4147 4306 518 Size: 648 1594 1028 359 159 2042 Fragments arranged by size: 2042 1594 1028 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3246 3923 5071 5316 1606 Size: 1640 677 1148 245 2120 Fragments arranged by size: 2120 1640 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 2237 513 Size: 552 957 215 4106 Fragments arranged by size: 4106 957 552 215 NdeI CA'TA_TG Cuts at: 387 387 Size: 5830 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5830 NotI GC'GGCC_GC Cuts at: 3277 3277 Size: 5830 NspI r_CATG'y Cuts at: 1132 1229 3188 4733 1132 Size: 97 1959 1545 2229 Fragments arranged by size: 2229 1959 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5830 PciI A'CATG_T Cuts at: 4729 4729 Size: 5830 PflMI CCAn_nnn'nTGG Cuts at: 1108 2404 1108 Size: 1296 4534 PfoI T'CCnGG_A Cuts at: 2158 2158 Size: 5830 PmeI GTTT'AAAC Cuts at: 3134 3134 Size: 5830 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3899 3931 5412 5444 3899 Size: 32 1481 32 4285 Fragments arranged by size: 4285 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5830 PsiI TTA'TAA Cuts at: 3400 3400 Size: 5830 PspOMI G'GGCC_C Cuts at: 2247 2247 Size: 5830 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3049 3081 3049 Size: 32 5798 PstI C_TGCA'G Cuts at: 830 2905 3026 3182 830 Size: 2075 121 156 3478 Fragments arranged by size: 3478 2075 156 121 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5830 SacI G_AGCT'C Cuts at: 3021 3054 3021 Size: 33 5797 SalI G'TCGA_C Cuts at: 1936 3172 1936 Size: 1236 4594 SapI GCTCTTCn'nnn_ Cuts at: 2270 2270 Size: 5830 SbfI CC_TGCA'GG Cuts at: 3182 3182 Size: 5830 ScaI AGT'ACT Cuts at: 4078 5790 4078 Size: 1712 4118 SfcI C'TryA_G Cuts at: 826 1045 2901 3022 3178 4313 4994 5185 826 Size: 219 1856 121 156 1135 681 191 1471 Fragments arranged by size: 1856 1471 1135 681 219 191 156 121 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5830 SmaI CCC'GGG Cuts at: 1872 3157 1872 Size: 1285 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3032 3934 4835 5097 820 Size: 197 926 1089 902 901 262 1553 Fragments arranged by size: 1553 1089 926 902 901 262 197 SnaBI TAC'GTA Cuts at: 493 3127 3621 493 Size: 2634 494 2702 Fragments arranged by size: 2702 2634 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5830 SphI G_CATG'C Cuts at: 1132 3188 1132 Size: 2056 3774 SspI AAT'ATT Cuts at: 5 52 2446 2886 3754 5 Size: 47 2394 440 868 2081 Fragments arranged by size: 2394 2081 868 440 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5830 StyI C'CwwG_G Cuts at: 513 1065 2022 2237 3269 513 Size: 552 957 215 1032 3074 Fragments arranged by size: 3074 1032 957 552 215 SwaI ATTT'AAAT Cuts at: 2760 2760 Size: 5830 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3857 3874 4027 4212 4657 325 Size: 1255 2277 17 153 185 445 1498 Fragments arranged by size: 2277 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4076 5788 96 Size: 274 80 33 51 1443 2099 1712 138 Fragments arranged by size: 2099 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5790 5822 5790 Size: 32 5798 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5830 XbaI T'CTAG_A Cuts at: 2635 3166 2635 Size: 531 5299 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5830 XmaI C'CCGG_G Cuts at: 1870 3155 1870 Size: 1285 4545 XmnI GAAnn'nnTTC Cuts at: 2896 3959 2896 Size: 1063 4767 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3638 276 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI XcmI