Restriction map for
pFN21ASDA1445
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# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AflIII AhdI AocI Aor51HI AscI
AseI AsnI Asp718I AspEI AvrII
BamHI BciVI BclI BfrI BglII
BlnI BsaBI BseAI BsiWI BsmI
BspDI BspEI BspLU11I Bst1107I BstBI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI Eam1105I Eco47III Eco81I EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI MamI MfeI
MflI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PmeI PshBI PvuI RsrII
SalI ScaI SfiI SfuI SgrAI
SnaBI SpeI SspI SwaI XbaI
XhoII
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# ONE CUT ENZYMES ( 55 enzymes) #
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AclI 1739 AACGTT AA^CGTT
AcsI 3289 GAATTC [GA]^AATT[CT]
AgeI 1291 ACCGGT A^CCGGT
ApoI 3289 GAATTC [GA]^AATT[CT]
Asp700I 582 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1636 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3113 TGGCCA TGG^CCA
BbeI 1568 GGCGCC GGCGC^C
BbrPI 1552 CACGTG CAC^GTG
BbvCI 645 CCTCAGC CCTCAGC(-5/-2)
BcgI 1745 GCAGCACACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2765 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2765 GCTCAGC GC^T[ACGT]AGC
BsaWI 1291 ACCGGT [AT]^CCGG[AT]
BsiEI 576 CGGCCG CG[GA][CT]^CG
Bsp1407I 3365 TGTACA T^GTACA
BspHI 1016 TCATGA T^CATGA
BsrDI 3347 CATTGC GCAATG(2/0)
BsrGI 3365 TGTACA T^GTACA
BssSI 1420 CTCGTG CACGAG(-5/-1)
BstEII 1844 GGTCACC G^GT[ACGT]ACC
BstPI 1844 GGTCACC G^GT[ACGT]ACC
BstXI 2893 CCAGAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2765 GCTCAGC GC^T[ACGT]AGC
EagI 576 CGGCCG C^GGCCG
EclXI 576 CGGCCG C^GGCCG
Eco52I 576 CGGCCG C^GGCCG
EcoO65I 1844 GGTCACC G^GT[ACGT]ACC
EcoRI 3289 GAATTC G^AATTC
KasI 1568 GGCGCC G^GCGCC
KspI 2474 CCGCGG CCGC^GG
MluNI 3113 TGGCCA TGG^CCA
MscI 3113 TGGCCA TGG^CCA
MseI 845 TTAA T^TAA
NarI 1568 GGCGCC GG^CGCC
PaeR7I 1753 CTCGAG C^TCGAG
PflFI 1636 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1291 ACCGGT A^CCGGT
PmaCI 1552 CACGTG CAC^GTG
PmlI 1552 CACGTG CAC^GTG
PshAI 2839 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1739 AACGTT AA^CGTT
RcaI 1016 TCATGA T^CATGA
SacII 2474 CCGCGG CCGC^GG
SapI 1617 GCTCTTC GCTCTTC(1/4)
SbfI 1323 CCTGCAGG CCTGCA^GG
SexAI 1430 ACCTGGT A^CC[AT]GGT
SfoI 1568 GGCGCC GGC^GCC
SmlI 1753 CTCGAG C^T[CT][GA]AG
Sse8387I 1323 CCTGCAGG CCTGCA^GG
SspBI 3365 TGTACA T^GTACA
Tru9I 845 TTAA T^TAA
Tth111I 1636 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1753 CTCGAG C^TCGAG
XmnI 582 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwI 1910 GATCC GGATC(4/5)
3318 GATCC
ApaI 1700 GGGCCC GGGCC^C
2577 GGGCCC
BfaI 152 CTAG C^TAG
3157 CTAG
BsaAI 553 TACGTG [CT]AC^GT[GA]
1552 CACGTG
BspMI 329 ACCTGC ACCTGC(4/8)
2401 ACCTGC
BsrBI 507 CCGCTC CCGCTC(-3/-3)
2556 GAGCGG
BssHII 1613 GCGCGC G^CGCGC
2408 GCGCGC
DrdI 2001 GACCCCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2748 GACCTCATGGTC
EaeI 576 CGGCCG [CT]^GGCC[GA]
3113 TGGCCA
MaeI 152 CTAG C^TAG
3157 CTAG
NaeI 574 GCCGGC GCC^GGC
2957 GCCGGC
NgoMIV 574 GCCGGC G^CCGGC
2957 GCCGGC
NspI 1690 GCATGC [GA]CATG^[CT]
2222 GCATGC
SphI 1690 GCATGC GCATG^C
2222 GCATGC
StuI 2885 AGGCCT AGG^CCT
3419 AGGCCT
Tsp509I 1264 AATT ^AATT
3290 AATT
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# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwNI ApaLI AspHI AvaI
AvaII AviII BanI BanII BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI