Restriction map for
pFN21ASDA1445
(Linear) (Six-base) MAPSORT of: KIAA1445.seq Check: 5330 from: 1 to: 3453
KIAA1445.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2410 3453
Size: 2410 1043
AclI AA'CG_TT
Cuts at: 0 1740 3453
Size: 1740 1713
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 454 1516 3453
Size: 423 31 1062 1937
Fragments arranged by size:
1937 1062 423 31
AgeI A'CCGG_T
Cuts at: 0 1291 3453
Size: 1291 2162
AleI CACnn'nnGTG
Cuts at: 0 926 3453
Size: 926 2527
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2116 2148 2242 2274 2374 2406 3453
Size: 2116 32 94 32 100 32 1047
Fragments arranged by size:
2116 1047 100 94 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 288 2210 2993 3453
Size: 288 1922 783 460
Fragments arranged by size:
1922 783 460 288
ApaI G_GGCC'C
Cuts at: 0 1704 2581 3453
Size: 1704 877 872
Fragments arranged by size:
1704 877 872
ApaLI G'TGCA_C
Cuts at: 0 621 2637 2928 3453
Size: 621 2016 291 525
Fragments arranged by size:
2016 621 525 291
ApoI r'AATT_y
Cuts at: 0 3289 3453
Size: 3289 164
AvaI C'yCGr_G
Cuts at: 0 368 1044 1378 1564 1703 1753 2299 2597
Size: 368 676 334 186 139 50 546 298
Cuts at: 2597 2848 3415 3453
Size: 251 567 38
Fragments arranged by size:
676 567 546 368 334 298 251 186
139 50 38
BanI G'GyrC_C
Cuts at: 0 1276 1474 1486 1568 2160 3256 3453
Size: 1276 198 12 82 592 1096 197
Fragments arranged by size:
1276 1096 592 198 197 82 12
BanII G_rGCy'C
Cuts at: 0 272 369 740 1385 1580 1704 1908 2188
Size: 272 97 371 645 195 124 204 280
Cuts at: 2188 2581 2654 2946 2975 3142 3149 3453
Size: 393 73 292 29 167 7 304
Fragments arranged by size:
645 393 371 304 292 280 272 204
195 167 124 97 73 29 7
BbeI G_GCGC'C
Cuts at: 0 1572 3453
Size: 1572 1881
BbsI GAAGACnn'nnnn_
Cuts at: 0 118 332 535 3453
Size: 118 214 203 2918
Fragments arranged by size:
2918 214 203 118
BbvCI CC'TCA_GC
Cuts at: 0 646 3453
Size: 646 2807
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1734 1768 3453
Size: 1734 34 1685
Fragments arranged by size:
1734 1685 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1529 1566 2096 2884 2897 3453
Size: 1529 37 530 788 13 556
Fragments arranged by size:
1529 788 556 530 37 13
BlpI GC'TnA_GC
Cuts at: 0 2766 3453
Size: 2766 687
Bme1580I G_kGCm'C
Cuts at: 0 625 1489 1704 2165 2376 2581 2641 2932
Size: 625 864 215 461 211 205 60 291
Cuts at: 2932 3453
Size: 521
Fragments arranged by size:
864 625 521 461 291 215 211 205
60
BmgBI CAC'GTC
Cuts at: 0 975 2033 2291 3453
Size: 975 1058 258 1162
Fragments arranged by size:
1162 1058 975 258
BmrI ACTGGGnnnn_n'
Cuts at: 0 2716 3453
Size: 2716 737
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 504 1779 3453
Size: 504 1275 1674
Fragments arranged by size:
1674 1275 504
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 246 383 474 1015 1561 1678 1968
Size: 228 18 137 91 541 546 117 290
Cuts at: 1968 2211 3045 3453
Size: 243 834 408
Fragments arranged by size:
834 546 541 408 290 243 228 137
117 91 18
Bpu10I CC'TnA_GC
Cuts at: 0 76 298 646 3453
Size: 76 222 348 2807
Fragments arranged by size:
2807 348 222 76
BsaI GGTCTCn'nnnn_
Cuts at: 0 348 1282 2762 3453
Size: 348 934 1480 691
Fragments arranged by size:
1480 934 691 348
BsaAI yAC'GTr
Cuts at: 0 555 1554 3453
Size: 555 999 1899
Fragments arranged by size:
1899 999 555
BsaHI Gr'CG_yC
Cuts at: 0 1569 1640 2235 2338 2504 3453
Size: 1569 71 595 103 166 949
Fragments arranged by size:
1569 949 595 166 103 71
BsaWI w'CCGG_w
Cuts at: 0 1291 3453
Size: 1291 2162
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2116 2146 2242 2272 2374 2404 3453
Size: 2116 30 96 30 102 30 1049
Fragments arranged by size:
2116 1049 102 96 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 550 553 714 1558 2878 2965 2983 3085
Size: 550 3 161 844 1320 87 18 102
Cuts at: 3085 3453
Size: 368
Fragments arranged by size:
1320 844 550 368 161 102 87 18
3
BseYI C'CCAG_C
Cuts at: 0 63 214 271 380 1721 2564 2610 3453
Size: 63 151 57 109 1341 843 46 843
Fragments arranged by size:
1341 843 843 151 109 63 57 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1384 1456 1534 1585 2046 2220 2793 2839
Size: 1384 72 78 51 461 174 573 46
Cuts at: 2839 3107 3453
Size: 268 346
Fragments arranged by size:
1384 573 461 346 268 174 78 72
51 46
BsiEI CG_ry'CG
Cuts at: 0 579 3453
Size: 579 2874
BsiHKAI G_wGCw'C
Cuts at: 0 625 740 923 1868 1908 2539 2641 2932
Size: 625 115 183 945 40 631 102 291
Cuts at: 2932 2975 3453
Size: 43 478
Fragments arranged by size:
945 631 625 478 291 183 115 102
43 40
BsmBI CGTCTCn'nnnn_
Cuts at: 0 26 1628 2452 3453
Size: 26 1602 824 1001
Fragments arranged by size:
1602 1001 824 26
Bsp1286I G_dGCh'C
Cuts at: 0 272 369 625 740 923 1385 1489 1580
Size: 272 97 256 115 183 462 104 91
Cuts at: 1580 1704 1868 1908 2165 2188 2376 2539 2581
Size: 124 164 40 257 23 188 163 42
Cuts at: 2581 2641 2654 2932 2946 2975 3142 3149 3453
Size: 60 13 278 14 29 167 7 304
Fragments arranged by size:
462 304 278 272 257 256 188 183
167 164 163 124 115 104 97 91
60 42 40 29 23 14 13 7
BspHI T'CATG_A
Cuts at: 0 1016 3453
Size: 1016 2437
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 338 2410 3453
Size: 338 2072 1043
Fragments arranged by size:
2072 1043 338
BsrBI CCG'CTC
Cuts at: 0 509 2558 3453
Size: 509 2049 895
Fragments arranged by size:
2049 895 509
BsrDI GCAATG_nn'
Cuts at: 0 3346 3453
Size: 3346 107
BsrFI r'CCGG_y
Cuts at: 0 574 1291 1368 2957 3079 3453
Size: 574 717 77 1589 122 374
Fragments arranged by size:
1589 717 574 374 122 77
BsrGI T'GTAC_A
Cuts at: 0 3365 3453
Size: 3365 88
BssHII G'CGCG_C
Cuts at: 0 1613 2408 3453
Size: 1613 795 1045
Fragments arranged by size:
1613 1045 795
BssSI C'ACGA_G
Cuts at: 0 1420 3453
Size: 1420 2033
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1557 2261 2433 2875 3128 3453
Size: 1557 704 172 442 253 325
Fragments arranged by size:
1557 704 442 325 253 172
BstEII G'GTnAC_C
Cuts at: 0 1844 3453
Size: 1844 1609
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2900 3453
Size: 2900 553
BtgI C'CryG_G
Cuts at: 0 314 337 749 1508 1527 1840 1849 2005
Size: 314 23 412 759 19 313 9 156
Cuts at: 2005 2091 2341 2474 2581 3116 3284 3453
Size: 86 250 133 107 535 168 169
Fragments arranged by size:
759 535 412 314 313 250 169 168
156 133 107 86 23 19 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1128 1956 2696 3453
Size: 1128 828 740 757
Fragments arranged by size:
1128 828 757 740
BtsI GCAGTG_nn'
Cuts at: 0 223 282 807 818 920 3167 3180 3453
Size: 223 59 525 11 102 2247 13 273
Fragments arranged by size:
2247 525 273 223 102 59 13 11
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 842 877 3453
Size: 842 35 2576
Fragments arranged by size:
2576 842 35
DraIII CAC_nnn'GTG
Cuts at: 0 482 1422 2553 2875 3111 3453
Size: 482 940 1131 322 236 342
Fragments arranged by size:
1131 940 482 342 322 236
DrdI GACnn_nn'nnGTC
Cuts at: 0 2007 2754 3453
Size: 2007 747 699
Fragments arranged by size:
2007 747 699
EaeI y'GGCC_r
Cuts at: 0 576 3113 3453
Size: 576 2537 340
Fragments arranged by size:
2537 576 340
EagI C'GGCC_G
Cuts at: 0 576 3453
Size: 576 2877
EarI CTCTTCn'nnn_
Cuts at: 0 442 470 1348 1624 3453
Size: 442 28 878 276 1829
Fragments arranged by size:
1829 878 442 276 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2047 3266 3453
Size: 2047 1219 187
Fragments arranged by size:
2047 1219 187
EcoICRI GAG'CTC
Cuts at: 0 738 1906 2973 3453
Size: 738 1168 1067 480
Fragments arranged by size:
1168 1067 738 480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 246 383 423 454 474 1015 1516
Size: 228 18 137 40 31 20 541 501
Cuts at: 1516 1561 1678 1968 2211 3045 3453
Size: 45 117 290 243 834 408
Fragments arranged by size:
834 541 501 408 290 243 228 137
117 45 40 31 20 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 994 1435 2512 3453
Size: 994 441 1077 941
Fragments arranged by size:
1077 994 941 441
EcoO109I rG'GnC_Cy
Cuts at: 0 166 187 774 1053 1104 1650 1700 1707
Size: 166 21 587 279 51 546 50 7
Cuts at: 1707 1941 2465 2517 2577 2664 3453
Size: 234 524 52 60 87 789
Fragments arranged by size:
789 587 546 524 279 234 166 87
60 52 51 50 21 7
EcoRI G'AATT_C
Cuts at: 0 3289 3453
Size: 3289 164
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3330 3453
Size: 3330 123
FspI TGC'GCA
Cuts at: 0 1585 1594 2527 3453
Size: 1585 9 933 926
Fragments arranged by size:
1585 933 926 9
HaeII r_GCGC'y
Cuts at: 0 1341 1504 1572 1610 2023 2993 3453
Size: 1341 163 68 38 413 970 460
Fragments arranged by size:
1341 970 460 413 163 68 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 551 583 1625 1657 2454 2486 3453
Size: 551 32 1042 32 797 32 967
Fragments arranged by size:
1042 967 797 551 32 32 32
KasI G'GCGC_C
Cuts at: 0 1568 3453
Size: 1568 1885
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1790 3453
Size: 1790 1663
MscI TGG'CCA
Cuts at: 0 3115 3453
Size: 3115 338
MslI CAynn'nnrTG
Cuts at: 0 926 1015 1871 2107 3283 3453
Size: 926 89 856 236 1176 170
Fragments arranged by size:
1176 926 856 236 170 89
MspA1I CmG'CkG
Cuts at: 0 288 384 683 715 1934 2388 2476 2531
Size: 288 96 299 32 1219 454 88 55
Cuts at: 2531 3453
Size: 922
Fragments arranged by size:
1219 922 454 299 288 96 88 55
32
NaeI GCC'GGC
Cuts at: 0 576 2959 3453
Size: 576 2383 494
Fragments arranged by size:
2383 576 494
NarI GG'CG_CC
Cuts at: 0 1569 3453
Size: 1569 1884
NcoI C'CATG_G
Cuts at: 0 1840 1849 3284 3453
Size: 1840 9 1435 169
Fragments arranged by size:
1840 1435 169 9
NgoMIV G'CCGG_C
Cuts at: 0 574 2957 3453
Size: 574 2383 496
Fragments arranged by size:
2383 574 496
NspI r_CATG'y
Cuts at: 0 1694 2226 3453
Size: 1694 532 1227
Fragments arranged by size:
1694 1227 532
PasI CC'CwG_GG
Cuts at: 0 46 729 1226 2980 3453
Size: 46 683 497 1754 473
Fragments arranged by size:
1754 683 497 473 46
PflMI CCAn_nnn'nTGG
Cuts at: 0 482 2171 3111 3453
Size: 482 1689 940 342
Fragments arranged by size:
1689 940 482 342
PfoI T'CCnGG_A
Cuts at: 0 144 400 1984 2188 3453
Size: 144 256 1584 204 1265
Fragments arranged by size:
1584 1265 256 204 144
PmlI CAC'GTG
Cuts at: 0 1554 3453
Size: 1554 1899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2116 2148 2242 2274 2374 2406 3453
Size: 2116 32 94 32 100 32 1047
Fragments arranged by size:
2116 1047 100 94 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 774 1053 1104 1650 1707 2465 2517 3453
Size: 774 279 51 546 57 758 52 936
Fragments arranged by size:
936 774 758 546 279 57 52 51
PshAI GACnn'nnGTC
Cuts at: 0 2843 3453
Size: 2843 610
PspOMI G'GGCC_C
Cuts at: 0 1700 2577 3453
Size: 1700 877 876
Fragments arranged by size:
1700 877 876
PspXI vC'TCGA_Gb
Cuts at: 0 1753 3453
Size: 1753 1700
PstI C_TGCA'G
Cuts at: 0 162 335 1079 1328 2438 3453
Size: 162 173 744 249 1110 1015
Fragments arranged by size:
1110 1015 744 249 173 162
PvuII CAG'CTG
Cuts at: 0 288 384 715 3453
Size: 288 96 331 2738
Fragments arranged by size:
2738 331 288 96
SacI G_AGCT'C
Cuts at: 0 740 1908 2975 3453
Size: 740 1168 1067 478
Fragments arranged by size:
1168 1067 740 478
SacII CC_GC'GG
Cuts at: 0 2477 3453
Size: 2477 976
SanDI GG'GwC_CC
Cuts at: 0 774 1650 1707 3453
Size: 774 876 57 1746
Fragments arranged by size:
1746 876 774 57
SapI GCTCTTCn'nnn_
Cuts at: 0 1624 3453
Size: 1624 1829
SbfI CC_TGCA'GG
Cuts at: 0 1328 3453
Size: 1328 2125
SexAI A'CCwGG_T
Cuts at: 0 1430 3453
Size: 1430 2023
SfcI C'TryA_G
Cuts at: 0 158 331 1075 1324 2434 3030 3453
Size: 158 173 744 249 1110 596 423
Fragments arranged by size:
1110 744 596 423 249 173 158
SfoI GGC'GCC
Cuts at: 0 1570 3453
Size: 1570 1883
SmaI CCC'GGG
Cuts at: 0 370 1046 1566 1705 2599 3453
Size: 370 676 520 139 894 854
Fragments arranged by size:
894 854 676 520 370 139
SmlI C'TyrA_G
Cuts at: 0 1753 3453
Size: 1753 1700
SphI G_CATG'C
Cuts at: 0 1694 2226 3453
Size: 1694 532 1227
Fragments arranged by size:
1694 1227 532
SrfI GCCC'GGGC
Cuts at: 0 1046 3453
Size: 1046 2407
StuI AGG'CCT
Cuts at: 0 2887 3421 3453
Size: 2887 534 32
Fragments arranged by size:
2887 534 32
StyI C'CwwG_G
Cuts at: 0 1840 1849 3284 3453
Size: 1840 9 1435 169
Fragments arranged by size:
1840 1435 169 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1408 2470 3297 3453
Size: 1408 1062 827 156
Fragments arranged by size:
1408 1062 827 156
TatI w'GTAC_w
Cuts at: 0 941 1461 3275 3365 3453
Size: 941 520 1814 90 88
Fragments arranged by size:
1814 941 520 90 88
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 923 955 1785 1817 3453
Size: 923 32 830 32 1636
Fragments arranged by size:
1636 923 830 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1639 3453
Size: 1639 1814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 43 633 1195 1847 3453
Size: 43 590 562 652 1606
Fragments arranged by size:
1606 652 590 562 43
XhoI C'TCGA_G
Cuts at: 0 1753 3453
Size: 1753 1700
XmaI C'CCGG_G
Cuts at: 0 368 1044 1564 1703 2597 3453
Size: 368 676 520 139 894 856
Fragments arranged by size:
894 856 676 520 368 139
XmnI GAAnn'nnTTC
Cuts at: 0 586 3453
Size: 586 2867
Enzymes that do cut:
AarI AclI AcuI AgeI AleI AloI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI
PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII
SacI SacII SanDI SapI SbfI SexAI SfcI SfoI
SmaI SmlI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AflIII AhdI AlfI
AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI
BfrBI BglII BmtI BpuEI BsaBI BsiWI BsmI BspEI
BstBI BstYI BstZ17I Bsu36I ClaI DraI EcoRV FseI
FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PsiI
PsrI PvuI RsrII SalI ScaI SfiI SgfI SgrAI
SnaBI SpeI SspI SwaI XbaI ZraI