(Circular) (Six-base) MAPSORT of: pFN21ASDA1443.seq Check: 6753 from: 1 to: 6376 pFN21ASDA1443 6376 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2238 3493 2238 Size: 1255 5121 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4186 278 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2403 3719 1937 Size: 466 1316 4594 Fragments arranged by size: 4594 1316 466 Acc65I G'GTAC_C Cuts at: 3697 3697 Size: 6376 AclI AA'CG_TT Cuts at: 1563 4503 4876 1563 Size: 2940 373 3063 Fragments arranged by size: 3063 2940 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2091 2227 2391 4439 5823 6091 1632 Size: 459 136 164 2048 1384 268 1917 Fragments arranged by size: 2048 1917 1384 459 268 164 136 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6179 AflIII A'CryG_T Cuts at: 1203 5275 6252 1203 Size: 4072 977 1327 Fragments arranged by size: 4072 1327 977 AgeI A'CCGG_T Cuts at: 5207 5207 Size: 6376 AhdI GACnn_n'nnGTC Cuts at: 5105 5105 Size: 6376 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6376 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6376 AlwNI CAG_nnn'CTG Cuts at: 2905 3296 3508 4119 5691 2905 Size: 391 212 611 1572 3590 Fragments arranged by size: 3590 1572 611 391 212 ApaLI G'TGCA_C Cuts at: 4433 5589 4433 Size: 1156 5220 ApoI r'AATT_y Cuts at: 1493 3685 3911 4063 6172 1493 Size: 2192 226 152 2109 1697 Fragments arranged by size: 2192 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2825 4930 160 Size: 872 1793 2105 1606 Fragments arranged by size: 2105 1793 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 AvaI C'yCGr_G Cuts at: 1870 1943 2048 2388 2394 2799 3701 1870 Size: 73 105 340 6 405 902 4545 Fragments arranged by size: 4545 902 405 340 105 73 6 BamHI G'GATC_C Cuts at: 3706 4073 3706 Size: 367 6009 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 2476 3299 3697 5152 Size: 325 217 627 303 386 823 398 1455 Cuts at: 5152 618 Size: 1842 Fragments arranged by size: 1842 1455 823 627 398 386 325 303 217 BanII G_rGCy'C Cuts at: 1391 2145 2395 2586 3695 1391 Size: 754 250 191 1109 4072 Fragments arranged by size: 4072 1109 754 250 191 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5405 BbvCI CC'TCA_GC Cuts at: 3534 3543 3534 Size: 9 6367 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4567 4601 1903 Size: 34 2630 34 3678 Fragments arranged by size: 3678 2630 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4268 5478 4268 Size: 1210 5166 BclI T'GATC_A Cuts at: 1548 3657 1548 Size: 2109 4267 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2050 3022 4987 Size: 107 122 71 631 605 378 972 1965 Cuts at: 4987 136 Size: 1525 Fragments arranged by size: 1965 1525 972 631 605 378 122 107 71 BglII A'GATC_T Cuts at: 6371 6371 Size: 6376 BlpI GC'TnA_GC Cuts at: 3491 3793 3491 Size: 302 6074 Bme1580I G_kGCm'C Cuts at: 1790 2079 3166 3304 4437 5593 1790 Size: 289 1087 138 1133 1156 2573 Fragments arranged by size: 2573 1156 1133 1087 289 138 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6376 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2970 3439 4107 Size: 416 53 83 399 24 1553 469 668 Cuts at: 4107 5065 442 Size: 958 1753 Fragments arranged by size: 1753 1553 958 668 469 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6376 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2602 2602 Size: 6376 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2472 2484 2924 5036 1239 Size: 1233 12 440 2112 2579 Fragments arranged by size: 2579 2112 1233 440 12 Bpu10I CC'TnA_GC Cuts at: 3534 3543 3534 Size: 9 6367 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4501 5366 5664 4501 Size: 865 298 5213 Fragments arranged by size: 5213 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2126 5039 882 Size: 617 627 2913 2219 Fragments arranged by size: 2913 2219 627 617 BsaAI yAC'GTr Cuts at: 493 1204 3673 4167 493 Size: 711 2469 494 2702 Fragments arranged by size: 2702 2469 711 494 BsaBI GATnn'nnATC Cuts at: 6370 6370 Size: 6376 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4183 4565 5200 275 Size: 53 83 186 936 2650 382 635 1451 Fragments arranged by size: 2650 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3403 4809 5207 5481 5628 6039 3403 Size: 1406 398 274 147 411 3740 Fragments arranged by size: 3740 1406 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2083 2113 1178 Size: 30 165 30 680 30 5441 Fragments arranged by size: 5441 680 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2183 2472 2475 2486 2594 2861 3593 3596 Size: 997 289 3 11 108 267 732 3 Cuts at: 3596 3629 3632 3635 3638 1186 Size: 33 3 3 3 3924 Fragments arranged by size: 3924 997 732 289 267 108 33 11 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2034 2261 2337 2787 3319 3498 5579 6075 2034 Size: 227 76 450 532 179 2081 496 2335 Fragments arranged by size: 2335 2081 532 496 450 227 179 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5803 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3826 4587 5615 1540 Size: 275 186 1825 761 1028 2301 Fragments arranged by size: 2301 1825 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4437 4522 5593 4437 Size: 85 1071 5220 Fragments arranged by size: 5220 1071 85 BsmI GAATG_Cn' Cuts at: 2657 3887 3980 2657 Size: 1230 93 5053 Fragments arranged by size: 5053 1230 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2145 2395 2586 3166 3304 3695 Size: 399 289 66 250 191 580 138 391 Cuts at: 3695 4437 4522 5593 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 580 399 391 289 250 191 138 85 66 BspEI T'CCGG_A Cuts at: 6039 6039 Size: 6376 BspHI T'CATG_A Cuts at: 4265 5995 4265 Size: 1730 4646 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2238 3493 3731 844 Size: 1394 1255 238 3489 Fragments arranged by size: 3489 1394 1255 238 BsrBI CCG'CTC Cuts at: 1394 1823 3351 4263 1394 Size: 429 1528 912 3507 Fragments arranged by size: 3507 1528 912 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2227 2274 4871 5045 65 Size: 1183 979 47 2597 174 1396 Fragments arranged by size: 2597 1396 1183 979 174 47 BsrFI r'CCGG_y Cuts at: 1700 1810 5020 5207 1700 Size: 110 3210 187 2869 Fragments arranged by size: 3210 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6376 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6376 BssSI C'ACGA_G Cuts at: 4436 5448 4436 Size: 1012 5364 BstAPI GCAn_nnn'nTGC Cuts at: 794 2276 2828 794 Size: 1482 552 4342 Fragments arranged by size: 4342 1482 552 BstBI TT'CG_AA Cuts at: 2007 5180 2007 Size: 3173 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6376 BstXI CCAn_nnnn'nTGG Cuts at: 2744 2744 Size: 6376 BstYI r'GATC_y Cuts at: 1972 3706 4073 4458 4475 5927 6013 6025 6371 Size: 1734 367 385 17 1452 86 12 346 Cuts at: 6371 1972 Size: 1977 Fragments arranged by size: 1977 1734 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2577 2577 Size: 6376 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4867 Fragments arranged by size: 4867 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5637 Fragments arranged by size: 5637 683 56 BtsI GCAGTG_nn' Cuts at: 2600 2656 2704 3892 4686 4706 2600 Size: 56 48 1188 794 20 4270 Fragments arranged by size: 4270 1188 794 56 48 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6376 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3254 3289 529 Size: 35 2690 35 3616 Fragments arranged by size: 3616 2690 35 35 DraI TTT'AAA Cuts at: 3680 3680 Size: 6376 DraIII CAC_nnn'GTG Cuts at: 2978 3170 2978 Size: 192 6184 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5383 809 Size: 507 4067 1802 Fragments arranged by size: 4067 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3823 4712 6341 Size: 54 1425 50 275 18 1993 889 1629 Cuts at: 6341 8 Size: 43 Fragments arranged by size: 1993 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3823 1537 Size: 275 2011 4090 Fragments arranged by size: 4090 2011 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2376 2878 2893 3564 3570 3606 4306 1353 Size: 1023 502 15 671 6 36 700 3423 Fragments arranged by size: 3423 1023 700 671 502 36 15 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4963 5337 5483 387 Size: 1064 138 3374 374 146 1280 Fragments arranged by size: 3374 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2091 2227 2391 2472 2484 2924 4439 Size: 393 459 136 164 81 12 440 1515 Cuts at: 4439 5036 5823 6091 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1515 787 597 459 440 393 268 164 136 81 12 EcoNI CCTnn'n_nnAGG Cuts at: 2708 3421 2708 Size: 713 5663 EcoO109I rG'GnC_Cy Cuts at: 2525 2729 2525 Size: 204 6172 EcoRI G'AATT_C Cuts at: 3685 4063 3685 Size: 378 5998 EcoRV GAT'ATC Cuts at: 3463 3463 Size: 6376 FspI TGC'GCA Cuts at: 4882 4882 Size: 6376 HaeII r_GCGC'y Cuts at: 1678 2344 5523 1678 Size: 666 3179 2531 Fragments arranged by size: 3179 2531 666 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2840 2872 3364 3396 5017 Size: 32 135 32 1268 32 492 32 1621 Cuts at: 5017 5049 5091 5123 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1621 1268 492 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3720 3966 669 Size: 988 281 1782 246 3079 Fragments arranged by size: 3079 1782 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6376 HpaI GTT'AAC Cuts at: 3966 3966 Size: 6376 KpnI G_GTAC'C Cuts at: 3701 3701 Size: 6376 MfeI C'AATT_G Cuts at: 2817 3470 3975 2817 Size: 653 505 5218 Fragments arranged by size: 5218 653 505 MluI A'CGCG_T Cuts at: 6252 6252 Size: 6376 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2555 5490 5674 1305 Size: 1250 2935 184 2007 Fragments arranged by size: 2935 2007 1250 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4554 Fragments arranged by size: 4554 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4334 4693 4852 518 Size: 648 3168 359 159 2042 Fragments arranged by size: 3168 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2118 2138 2349 3124 3542 3792 4469 5617 Size: 512 20 211 775 418 250 677 1148 Cuts at: 5617 5862 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 775 677 512 418 250 245 211 20 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4867 Fragments arranged by size: 4867 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6376 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6376 NotI GC'GGCC_GC Cuts at: 3823 3823 Size: 6376 NspI r_CATG'y Cuts at: 1132 1229 3734 5279 1132 Size: 97 2505 1545 2229 Fragments arranged by size: 2505 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6376 PciI A'CATG_T Cuts at: 5275 5275 Size: 6376 PflMI CCAn_nnn'nTGG Cuts at: 1108 2905 1108 Size: 1797 4579 PfoI T'CCnGG_A Cuts at: 2762 2762 Size: 6376 PmeI GTTT'AAAC Cuts at: 3680 3680 Size: 6376 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2217 2249 4445 4477 5958 5990 2217 Size: 32 2196 32 1481 32 2603 Fragments arranged by size: 2603 2196 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2525 2729 2525 Size: 204 6172 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6376 PsiI TTA'TAA Cuts at: 3946 3946 Size: 6376 PspXI vC'TCGA_Gb Cuts at: 2388 2388 Size: 6376 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2973 3005 2973 Size: 32 6344 PstI C_TGCA'G Cuts at: 830 3728 830 Size: 2898 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6376 PvuII CAG'CTG Cuts at: 3124 3542 3124 Size: 418 5958 SalI G'TCGA_C Cuts at: 1936 3718 1936 Size: 1782 4594 SanDI GG'GwC_CC Cuts at: 2729 2729 Size: 6376 SbfI CC_TGCA'GG Cuts at: 3728 3728 Size: 6376 ScaI AGT'ACT Cuts at: 4624 6336 4624 Size: 1712 4664 SexAI A'CCwGG_T Cuts at: 3043 3295 3043 Size: 252 6124 SfcI C'TryA_G Cuts at: 826 1045 2174 3724 4859 5540 5731 826 Size: 219 1129 1550 1135 681 191 1471 Fragments arranged by size: 1550 1471 1135 1129 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6376 SmaI CCC'GGG Cuts at: 1872 2050 3703 1872 Size: 178 1653 4545 Fragments arranged by size: 4545 1653 178 SmlI C'TyrA_G Cuts at: 820 1017 1943 2388 4480 5381 5643 820 Size: 197 926 445 2092 901 262 1553 Fragments arranged by size: 2092 1553 926 901 445 262 197 SnaBI TAC'GTA Cuts at: 493 3673 4167 493 Size: 3180 494 2702 Fragments arranged by size: 3180 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6376 SphI G_CATG'C Cuts at: 1132 3734 1132 Size: 2602 3774 SrfI GCCC'GGGC Cuts at: 2050 2050 Size: 6376 SspI AAT'ATT Cuts at: 5 52 4300 5 Size: 47 4248 2081 Fragments arranged by size: 4248 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6376 StyI C'CwwG_G Cuts at: 513 1065 2022 3019 3417 3815 513 Size: 552 957 997 398 398 3074 Fragments arranged by size: 3074 997 957 552 398 398 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2989 3512 4403 4420 4573 4758 5203 Size: 1255 1409 523 891 17 153 185 445 Cuts at: 5203 325 Size: 1498 Fragments arranged by size: 1498 1409 1255 891 523 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4622 6334 96 Size: 274 80 33 51 1443 2645 1712 138 Fragments arranged by size: 2645 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2083 2115 6336 6368 2083 Size: 32 4221 32 2091 Fragments arranged by size: 4221 2091 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6376 XbaI T'CTAG_A Cuts at: 3559 3712 3559 Size: 153 6223 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3121 3121 Size: 6376 XhoI C'TCGA_G Cuts at: 1943 2388 1943 Size: 445 5931 XmaI C'CCGG_G Cuts at: 1870 2048 3701 1870 Size: 178 1653 4545 Fragments arranged by size: 4545 1653 178 XmnI GAAnn'nnTTC Cuts at: 4505 4505 Size: 6376 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4184 276 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI RsrII SacI SacII SapI SfiI SfoI SwaI