(Circular) (Six-base) MAPSORT of: pFN21ASDA1442.seq Check: 9836 from: 1 to: 6349 pFN21ASDA1442 6349 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4159 278 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3021 3692 1937 Size: 1084 671 4594 Fragments arranged by size: 4594 1084 671 Acc65I G'GTAC_C Cuts at: 3670 3670 Size: 6349 AclI AA'CG_TT Cuts at: 1563 4476 4849 1563 Size: 2913 373 3063 Fragments arranged by size: 3063 2913 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2075 2630 3149 3215 3292 4412 5796 6064 Size: 443 555 519 66 77 1120 1384 268 Cuts at: 6064 1632 Size: 1917 Fragments arranged by size: 1917 1384 1120 555 519 443 268 77 66 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6152 AflIII A'CryG_T Cuts at: 1203 2683 2706 5248 6225 1203 Size: 1480 23 2542 977 1327 Fragments arranged by size: 2542 1480 1327 977 23 AgeI A'CCGG_T Cuts at: 5180 5180 Size: 6349 AhdI GACnn_n'nnGTC Cuts at: 2413 5078 2413 Size: 2665 3684 AleI CACnn'nnGTG Cuts at: 1166 2666 1166 Size: 1500 4849 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6349 AlwNI CAG_nnn'CTG Cuts at: 3187 4092 5664 3187 Size: 905 1572 3872 Fragments arranged by size: 3872 1572 905 ApaI G_GGCC'C Cuts at: 3354 3354 Size: 6349 ApaLI G'TGCA_C Cuts at: 2714 4406 5562 2714 Size: 1692 1156 3501 Fragments arranged by size: 3501 1692 1156 ApoI r'AATT_y Cuts at: 1493 3658 3884 4036 6145 1493 Size: 2165 226 152 2109 1697 Fragments arranged by size: 2165 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 2744 3471 2744 Size: 727 5622 AseI AT'TA_AT Cuts at: 160 1032 4903 160 Size: 872 3871 1606 Fragments arranged by size: 3871 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 AvaI C'yCGr_G Cuts at: 1870 1943 2309 2798 2951 3101 3245 3268 3346 Size: 73 366 489 153 150 144 23 78 Cuts at: 3346 3353 3674 1870 Size: 7 321 4545 Fragments arranged by size: 4545 489 366 321 153 150 144 78 73 23 7 BamHI G'GATC_C Cuts at: 2334 3679 4046 2334 Size: 1345 367 4637 Fragments arranged by size: 4637 1345 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2822 3670 5125 618 Size: 325 217 627 1035 848 1455 1842 Fragments arranged by size: 1842 1455 1035 848 627 325 217 BanII G_rGCy'C Cuts at: 1391 2090 2310 2904 3042 3354 3614 3668 1391 Size: 699 220 594 138 312 260 54 4072 Fragments arranged by size: 4072 699 594 312 260 220 138 54 BbeI G_GCGC'C Cuts at: 2826 2826 Size: 6349 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5378 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4540 4574 1903 Size: 34 2603 34 3678 Fragments arranged by size: 3678 2603 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4241 5451 4241 Size: 1210 5139 BclI T'GATC_A Cuts at: 1548 1548 Size: 6349 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2941 3146 3462 Size: 107 122 71 631 605 1269 205 316 Cuts at: 3462 4960 136 Size: 1498 1525 Fragments arranged by size: 1525 1498 1269 631 605 316 205 122 107 71 BglII A'GATC_T Cuts at: 6344 6344 Size: 6349 BlpI GC'TnA_GC Cuts at: 3766 3766 Size: 6349 Bme1580I G_kGCm'C Cuts at: 1790 2718 3171 3354 4410 5566 1790 Size: 928 453 183 1056 1156 2573 Fragments arranged by size: 2573 1156 1056 928 453 183 BmgBI CAC'GTC Cuts at: 1318 3211 1318 Size: 1893 4456 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4080 5038 442 Size: 416 53 83 399 24 2663 958 1753 Fragments arranged by size: 2663 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6349 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2492 2560 2492 Size: 68 6281 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5009 1239 Size: 3770 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2561 4474 5339 5637 2561 Size: 1913 865 298 3273 Fragments arranged by size: 3273 1913 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3090 5012 882 Size: 617 1591 1922 2219 Fragments arranged by size: 2219 1922 1591 617 BsaAI yAC'GTr Cuts at: 493 1204 2395 2686 3646 4140 493 Size: 711 1191 291 960 494 2702 Fragments arranged by size: 2702 1191 960 711 494 291 BsaBI GATnn'nnATC Cuts at: 6343 6343 Size: 6349 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2127 2540 2823 2933 Size: 53 83 186 936 594 413 283 110 Cuts at: 2933 3339 4156 4538 5173 275 Size: 406 817 382 635 1451 Fragments arranged by size: 1451 936 817 635 594 413 406 382 283 186 110 83 53 BsaWI w'CCGG_w Cuts at: 2511 2529 4782 5180 5454 5601 6012 2511 Size: 18 2253 398 274 147 411 2848 Fragments arranged by size: 2848 2253 411 398 274 147 18 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2708 2738 3266 3296 1178 Size: 30 165 30 1305 30 528 30 4231 Fragments arranged by size: 4231 1305 528 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 1186 Size: 889 5460 BseYI C'CCAG_C Cuts at: 3407 3509 5552 6048 3407 Size: 102 2043 496 3708 Fragments arranged by size: 3708 2043 496 102 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2515 2948 1163 Size: 573 779 433 4564 Fragments arranged by size: 4564 779 573 433 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2242 3013 3141 3799 4560 5588 Size: 275 186 241 771 128 658 761 1028 Cuts at: 5588 1540 Size: 2301 Fragments arranged by size: 2301 1028 771 761 658 275 241 186 128 BsiHKAI G_wGCw'C Cuts at: 2170 2718 2889 2904 4410 4495 5566 2170 Size: 548 171 15 1506 85 1071 2953 Fragments arranged by size: 2953 1506 1071 548 171 85 15 BsmI GAATG_Cn' Cuts at: 2620 3860 3953 2620 Size: 1240 93 5016 Fragments arranged by size: 5016 1240 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2405 2531 2405 Size: 126 6223 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2170 2310 2718 2889 2904 3042 Size: 399 300 80 140 408 171 15 138 Cuts at: 3042 3171 3354 3614 3668 4410 4495 5566 1391 Size: 129 183 260 54 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 408 399 300 260 183 171 140 138 129 85 80 54 15 BspEI T'CCGG_A Cuts at: 6012 6012 Size: 6349 BspHI T'CATG_A Cuts at: 4238 5968 4238 Size: 1730 4619 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2857 3704 844 Size: 2013 847 3489 Fragments arranged by size: 3489 2013 847 BsrBI CCG'CTC Cuts at: 1394 1823 3253 3429 4236 1394 Size: 429 1430 176 807 3507 Fragments arranged by size: 3507 1430 807 429 176 BsrDI GCAATG_nn' Cuts at: 65 1248 4844 5018 65 Size: 1183 3596 174 1396 Fragments arranged by size: 3596 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2078 2241 2499 3485 4993 5180 1700 Size: 110 268 163 258 986 1508 187 2869 Fragments arranged by size: 2869 1508 986 268 258 187 163 110 BsrGI T'GTAC_A Cuts at: 96 3225 96 Size: 3129 3220 BssHII G'CGCG_C Cuts at: 1925 2744 3199 3368 3471 1925 Size: 819 455 169 103 4803 Fragments arranged by size: 4803 819 455 169 103 BssSI C'ACGA_G Cuts at: 2163 2888 4409 5421 2163 Size: 725 1521 1012 3091 Fragments arranged by size: 3091 1521 1012 725 BstAPI GCAn_nnn'nTGC Cuts at: 794 2502 3003 794 Size: 1708 501 4140 Fragments arranged by size: 4140 1708 501 BstBI TT'CG_AA Cuts at: 2007 5153 2007 Size: 3146 3203 BstEII G'GTnAC_C Cuts at: 1184 2965 1184 Size: 1781 4568 BstYI r'GATC_y Cuts at: 1972 2334 3679 4046 4431 4448 5900 5986 5998 Size: 362 1345 367 385 17 1452 86 12 Cuts at: 5998 6344 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1345 385 367 362 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2198 2198 Size: 6349 BtgI C'CryG_G Cuts at: 513 1065 2022 2136 2256 2814 2939 3394 3614 Size: 552 957 114 120 558 125 455 220 Cuts at: 3614 513 Size: 3248 Fragments arranged by size: 3248 957 558 552 455 220 125 120 114 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3310 487 Size: 683 56 2084 3526 Fragments arranged by size: 3526 2084 683 56 BtsI GCAGTG_nn' Cuts at: 3865 4659 4679 3865 Size: 794 20 5535 Fragments arranged by size: 5535 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6349 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2815 2850 529 Size: 35 2251 35 4028 Fragments arranged by size: 4028 2251 35 35 DraI TTT'AAA Cuts at: 3653 3653 Size: 6349 DraIII CAC_nnn'GTG Cuts at: 2224 2224 Size: 6349 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5356 809 Size: 507 4040 1802 Fragments arranged by size: 4040 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2229 2691 2734 Size: 54 1425 50 275 18 399 462 43 Cuts at: 2734 3010 3138 3213 3321 3483 3796 4685 6314 Size: 276 128 75 108 162 313 889 1629 Cuts at: 6314 8 Size: 43 Fragments arranged by size: 1629 1425 889 462 399 313 276 275 162 128 108 75 54 50 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3010 3138 3796 1537 Size: 275 1198 128 658 4090 Fragments arranged by size: 4090 1198 658 275 128 EarI CTCTTCn'nnn_ Cuts at: 1353 2510 4279 1353 Size: 1157 1769 3423 Fragments arranged by size: 3423 1769 1157 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2330 3221 3274 4936 5310 5456 Size: 1064 138 741 891 53 1662 374 146 Cuts at: 5456 387 Size: 1280 Fragments arranged by size: 1662 1280 1064 891 741 374 146 138 53 EcoICRI GAG'CTC Cuts at: 2902 2902 Size: 6349 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2630 3149 3215 3292 4412 5009 Size: 393 443 555 519 66 77 1120 597 Cuts at: 5009 5796 6064 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1120 787 597 555 519 443 393 268 77 66 EcoNI CCTnn'n_nnAGG Cuts at: 2057 2057 Size: 6349 EcoO109I rG'GnC_Cy Cuts at: 3350 3504 3350 Size: 154 6195 EcoRI G'AATT_C Cuts at: 3658 4036 3658 Size: 378 5971 FseI GG_CCGG'CC Cuts at: 3489 3489 Size: 6349 FspI TGC'GCA Cuts at: 4855 4855 Size: 6349 HaeII r_GCGC'y Cuts at: 1678 2826 3559 5496 1678 Size: 1148 733 1937 2531 Fragments arranged by size: 2531 1937 1148 733 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2399 2431 4990 5022 5064 Size: 32 135 32 827 32 2559 32 42 Cuts at: 5064 5096 1373 Size: 32 2626 Fragments arranged by size: 2626 2559 827 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3693 3939 669 Size: 988 281 1755 246 3079 Fragments arranged by size: 3079 1755 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6349 HpaI GTT'AAC Cuts at: 3939 3939 Size: 6349 KasI G'GCGC_C Cuts at: 2822 2822 Size: 6349 KpnI G_GTAC'C Cuts at: 3674 3674 Size: 6349 MfeI C'AATT_G Cuts at: 3948 3948 Size: 6349 MluI A'CGCG_T Cuts at: 6225 6225 Size: 6349 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2217 2232 2724 5463 5647 1305 Size: 912 15 492 2739 184 2007 Fragments arranged by size: 2739 2007 912 492 184 15 MscI TGG'CCA Cuts at: 10 64 1489 1832 2231 3323 10 Size: 54 1425 343 399 1092 3036 Fragments arranged by size: 3036 1425 1092 399 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2666 2711 2924 4307 4666 4825 518 Size: 648 1500 45 213 1383 359 159 2042 Fragments arranged by size: 2042 1500 1383 648 359 213 159 45 MspA1I CmG'CkG Cuts at: 1606 2043 2068 2138 2941 3319 3375 3396 3765 Size: 437 25 70 803 378 56 21 369 Cuts at: 3765 4442 5590 5835 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 803 677 437 378 369 245 70 56 25 21 NaeI GCC'GGC Cuts at: 2080 3487 2080 Size: 1407 4942 NarI GG'CG_CC Cuts at: 2823 2823 Size: 6349 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4840 Fragments arranged by size: 4840 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6349 NgoMIV G'CCGG_C Cuts at: 2078 3485 2078 Size: 1407 4942 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6349 NotI GC'GGCC_GC Cuts at: 3796 3796 Size: 6349 NspI r_CATG'y Cuts at: 1132 1229 2638 2710 3707 5252 1132 Size: 97 1409 72 997 1545 2229 Fragments arranged by size: 2229 1545 1409 997 97 72 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6349 PciI A'CATG_T Cuts at: 2706 5248 2706 Size: 2542 3807 PflMI CCAn_nnn'nTGG Cuts at: 1108 2653 1108 Size: 1545 4804 PfoI T'CCnGG_A Cuts at: 2099 2121 2099 Size: 22 6327 PmeI GTTT'AAAC Cuts at: 3653 3653 Size: 6349 PmlI CAC'GTG Cuts at: 2686 2686 Size: 6349 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4418 4450 5931 5963 4418 Size: 32 1481 32 4804 Fragments arranged by size: 4804 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3504 3504 Size: 6349 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6349 PsiI TTA'TAA Cuts at: 3919 3919 Size: 6349 PspOMI G'GGCC_C Cuts at: 3350 3350 Size: 6349 PstI C_TGCA'G Cuts at: 830 3380 3701 830 Size: 2550 321 3478 Fragments arranged by size: 3478 2550 321 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6349 PvuII CAG'CTG Cuts at: 3375 3375 Size: 6349 SacI G_AGCT'C Cuts at: 2904 2904 Size: 6349 SacII CC_GC'GG Cuts at: 2139 2942 3397 2139 Size: 803 455 5091 Fragments arranged by size: 5091 803 455 SalI G'TCGA_C Cuts at: 1936 3691 1936 Size: 1755 4594 SanDI GG'GwC_CC Cuts at: 3504 3504 Size: 6349 SapI GCTCTTCn'nnn_ Cuts at: 2510 2510 Size: 6349 SbfI CC_TGCA'GG Cuts at: 3701 3701 Size: 6349 ScaI AGT'ACT Cuts at: 4597 6309 4597 Size: 1712 4637 SfcI C'TryA_G Cuts at: 826 1045 3068 3376 3697 4832 5513 5704 826 Size: 219 2023 308 321 1135 681 191 1471 Fragments arranged by size: 2023 1471 1135 681 321 308 219 191 SfoI GGC'GCC Cuts at: 2824 2824 Size: 6349 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6349 SmaI CCC'GGG Cuts at: 1872 2311 2800 2953 3247 3348 3676 1872 Size: 439 489 153 294 101 328 4545 Fragments arranged by size: 4545 489 439 328 294 153 101 SmlI C'TyrA_G Cuts at: 820 1017 1943 2576 4453 5354 5616 820 Size: 197 926 633 1877 901 262 1553 Fragments arranged by size: 1877 1553 926 901 633 262 197 SnaBI TAC'GTA Cuts at: 493 3646 4140 493 Size: 3153 494 2702 Fragments arranged by size: 3153 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6349 SphI G_CATG'C Cuts at: 1132 3707 1132 Size: 2575 3774 SspI AAT'ATT Cuts at: 5 52 4273 5 Size: 47 4221 2081 Fragments arranged by size: 4221 2081 47 StuI AGG'CCT Cuts at: 1517 3600 1517 Size: 2083 4266 StyI C'CwwG_G Cuts at: 513 1065 2022 3114 3549 3788 513 Size: 552 957 1092 435 239 3074 Fragments arranged by size: 3074 1092 957 552 435 239 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2316 2495 3291 4376 4393 4546 4731 Size: 1255 736 179 796 1085 17 153 185 Cuts at: 4731 5176 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1085 796 736 445 185 179 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3225 4595 6307 Size: 274 80 33 51 1443 1248 1370 1712 Cuts at: 6307 96 Size: 138 Fragments arranged by size: 1712 1443 1370 1248 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2506 2538 2708 2740 3266 3298 6309 6341 2506 Size: 32 170 32 526 32 3011 32 2514 Fragments arranged by size: 3011 2514 526 170 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2552 1316 Size: 1236 5113 XbaI T'CTAG_A Cuts at: 3685 3685 Size: 6349 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6349 XmaI C'CCGG_G Cuts at: 1870 2309 2798 2951 3245 3346 3674 1870 Size: 439 489 153 294 101 328 4545 Fragments arranged by size: 4545 489 439 328 294 153 101 XmnI GAAnn'nnTTC Cuts at: 4478 4478 Size: 6349 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4157 276 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I EcoRV FalI FspAI NruI NsiI PacI PspXI PsrI RsrII SexAI SfiI SrfI SwaI XcmI