Restriction map for
pFN21ASDA1441
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# NO CUT ENZYMES ( 87 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AgeI AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AvrII BamHI BclI BglII
BlnI BsaI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BsrGI BssHII
Bst1107I BstBI BstUI BstZ17I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoRI FbaI FseI
HgaI HincII HindII HindIII HpaI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SspI SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 2352 GACGTC GACGT^C
AflII 982 CTTAAG C^TTAAG
AflIII 3129 ACATGT A^C[GA][CT]GT
Aor51HI 2996 AGCGCT AGC^GCT
Asp700I 2970 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1779 GTGCTC G[AT]GC[AT]^C
AviII 1670 TGCGCA TGC^GCA
BalI 1208 TGGCCA TGG^CCA
BbeI 3026 GGCGCC GGCGC^C
BbrPI 1745 CACGTG CAC^GTG
BciVI 2287 GGATAC GTATCC(6/5)
BfrI 982 CTTAAG C^TTAAG
BlpI 302 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 302 GCTCAGC GC^T[ACGT]AGC
BsaBI 1054 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3341 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1779 GTGCTC G[AT]GC[AT]^C
BsmI 365 GAATGC GAATGC(1/-1)
BspLU11I 3129 ACATGT A^CATGT
BsrBI 1966 CCGCTC CCGCTC(-3/-3)
BsrFI 1591 ACCGGC [GA]^CCGG[CT]
BssSI 3046 CACGAG CACGAG(-5/-1)
BstEII 1680 GGTCACC G^GT[ACGT]ACC
BstPI 1680 GGTCACC G^GT[ACGT]ACC
CelII 302 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1591 ACCGGC [GA]^CCGG[CT]
DraIII 264 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2996 AGCGCT AGC^GCT
EcoO65I 1680 GGTCACC G^GT[ACGT]ACC
EcoRV 1467 GATATC GAT^ATC
EcoT22I 2176 ATGCAT ATGCA^T
FspI 1670 TGCGCA TGC^GCA
KasI 3026 GGCGCC G^GCGCC
MamI 1054 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1208 TGGCCA TGG^CCA
MscI 1208 TGGCCA TGG^CCA
NarI 3026 GGCGCC GG^CGCC
NheI 1102 GCTAGC G^CTAGC
NsiI 2176 ATGCAT ATGCA^T
PmaCI 1745 CACGTG CAC^GTG
PmlI 1745 CACGTG CAC^GTG
SapI 1635 GAAGAGC GCTCTTC(1/4)
SbfI 3071 CCTGCAGG CCTGCA^GG
ScaI 1228 AGTACT AGT^ACT
SexAI 132 ACCAGGT A^CC[AT]GGT
SfiI 347 GGCCTGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3026 GGCGCC GGC^GCC
Sse8387I 3071 CCTGCAGG CCTGCA^GG
Tsp509I 2236 AATT ^AATT
XmnI 2970 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcyI 2352 GACGTC G[GA]^CG[CT]C
3026 GGCGCC
ApaI 317 GGGCCC GGGCC^C
3161 GGGCCC
AspI 1476 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2497 GACACAGTC
BsaAI 1745 CACGTG [CT]AC^GT[GA]
2932 TACGTG
BsaHI 2352 GACGTC G[GA]^CG[CT]C
3026 GGCGCC
BspHI 1793 TCATGA T^CATGA
3371 TCATGA
BsrDI 947 GCAATG GCAATG(2/0)
2111 GCAATG
Hin1I 2352 GACGTC G[GA]^CG[CT]C
3026 GGCGCC
MslI 1236 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1741 CATGCACGTG
PflFI 1476 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2497 GACACAGTC
PflMI 766 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1815 CCAGATGGTGG
PpuMI 695 AGGTCCT [GA]G^G[AT]CC[CT]
1043 AGGTCCC
PvuII 1562 CAGCTG CAG^CTG
2301 CAGCTG
RcaI 1793 TCATGA T^CATGA
3371 TCATGA
TfiI 148 GAATC G^A[AT]TC
2184 GAATC
Tsp45I 1681 GTCAC ^GT[GC]AC
2903 GTCAC
Tth111I 1476 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2497 GACACAGTC
Van91I 766 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1815 CCAGATGGTGG
XcmI 2300 CCAGCTGTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2384 CCATCTGCCCCATGG
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI Tru9I TseI TspRI
TthHB8I XhoII