Restriction map for
pFN21ASDA1441
(Circular) (Six-base) MAPSORT of: pFN21ASDA1441.seq Check: 6034 from: 1 to: 8440
pFN21ASDA1441 8440 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3097 3716 3097
Size: 619 7821
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4379 6250 278
Size: 53 83 186 936 2843 1871 2468
Fragments arranged by size:
2843 2468 1871 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5783 1937
Size: 3846 4594
Acc65I G'GTAC_C
Cuts at: 5761 5761
Size: 8440
AclI AA'CG_TT
Cuts at: 1563 6567 6940 1563
Size: 5004 373 3063
Fragments arranged by size:
5004 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2196 4165 4782 5110 6503 7887 8155 1632
Size: 564 1969 617 328 1393 1384 268 1917
Fragments arranged by size:
1969 1917 1393 1384 617 564 328 268
AfeI AGC'GCT
Cuts at: 5021 5021
Size: 8440
AflII C'TTAA_G
Cuts at: 820 1017 3005 820
Size: 197 1988 6255
Fragments arranged by size:
6255 1988 197
AflIII A'CryG_T
Cuts at: 1203 5152 7339 8316 1203
Size: 3949 2187 977 1327
Fragments arranged by size:
3949 2187 1327 977
AgeI A'CCGG_T
Cuts at: 7271 7271
Size: 8440
AhdI GACnn_n'nnGTC
Cuts at: 7169 7169
Size: 8440
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8440
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3239 3566 4265 1184
Size: 2055 327 699 5359
Fragments arranged by size:
5359 2055 699 327
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2406 2438 2406
Size: 32 8408
AlwNI CAG_nnn'CTG
Cuts at: 2195 2795 3080 3461 3968 4772 6183 7755 2195
Size: 600 285 381 507 804 1411 1572 2880
Fragments arranged by size:
2880 1572 1411 804 600 507 381 285
ApaI G_GGCC'C
Cuts at: 2344 5188 2344
Size: 2844 5596
ApaLI G'TGCA_C
Cuts at: 6497 7653 6497
Size: 1156 7284
ApoI r'AATT_y
Cuts at: 1493 5749 5975 6127 8236 1493
Size: 4256 226 152 2109 1697
Fragments arranged by size:
4256 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6994 160
Size: 872 5962 1606
Fragments arranged by size:
5962 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8440
AvaI C'yCGr_G
Cuts at: 1870 1943 2297 2577 5217 5765 1870
Size: 73 354 280 2640 548 4545
Fragments arranged by size:
4545 2640 548 354 280 73
BamHI G'GATC_C
Cuts at: 5770 6137 5770
Size: 367 8073
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2462 2800 2826 3355 3422
Size: 325 217 627 675 338 26 529 67
Cuts at: 3422 4317 4709 5049 5420 5761 7216 618
Size: 895 392 340 371 341 1455 1842
Fragments arranged by size:
1842 1455 895 675 627 529 392 371
341 340 338 325 217 67 26
BanII G_rGCy'C
Cuts at: 1391 2344 2411 2590 2837 3581 3792 4833 5188
Size: 953 67 179 247 744 211 1041 355
Cuts at: 5188 5239 5710 5759 1391
Size: 51 471 49 4072
Fragments arranged by size:
4072 1041 953 744 471 355 247 211
179 67 51 49
BbeI G_GCGC'C
Cuts at: 5053 5053
Size: 8440
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2042 2184 2904 3727 4519 4782 5259
Size: 971 143 142 720 823 792 263 477
Cuts at: 5259 928
Size: 4109
Fragments arranged by size:
4109 971 823 792 720 477 263 143
142
BbvCI CC'TCA_GC
Cuts at: 3102 3120 3588 3972 3102
Size: 18 468 384 7570
Fragments arranged by size:
7570 468 384 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3679 3713 4190 4224 4280 4314 6631
Size: 34 1742 34 477 34 56 34 2317
Cuts at: 6631 6665 1903
Size: 34 3678
Fragments arranged by size:
3678 2317 1742 477 56 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4304 6332 7542 4304
Size: 2028 1210 5202
Fragments arranged by size:
5202 2028 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8440
BfrBI ATG'CAT
Cuts at: 4201 4201
Size: 8440
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2377 3098 3116
Size: 107 122 71 631 605 705 721 18
Cuts at: 3116 3278 4124 7051 136
Size: 162 846 2927 1525
Fragments arranged by size:
2927 1525 846 721 705 631 605 162
122 107 71 18
BglII A'GATC_T
Cuts at: 8435 8435
Size: 8440
BlpI GC'TnA_GC
Cuts at: 2326 5857 2326
Size: 3531 4909
Bme1580I G_kGCm'C
Cuts at: 1790 2344 2465 2805 2829 2859 3360 4145 4322
Size: 554 121 340 24 30 501 785 177
Cuts at: 4322 4406 4444 4604 4714 5188 5425 5530 6501
Size: 84 38 160 110 474 237 105 971
Cuts at: 6501 7657 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 971 785 554 501 474 340
237 177 160 121 110 105 84 38
30 24
BmgBI CAC'GTC
Cuts at: 1318 4294 1318
Size: 2976 5464
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2448 2783 3411
Size: 416 53 83 399 24 1031 335 628
Cuts at: 3411 3471 3496 5182 6171 7129 442
Size: 60 25 1686 989 958 1753
Fragments arranged by size:
1753 1686 1031 989 958 628 416 399
335 83 60 53 25 24
BmtI G_CTAG'C
Cuts at: 1056 3129 1056
Size: 2073 6367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2154 2232 2340 2728 2805 2971 3650 4070
Size: 915 78 108 388 77 166 679 420
Cuts at: 4070 4923 5361 5631 7100 1239
Size: 853 438 270 1469 2579
Fragments arranged by size:
2579 1469 915 853 679 438 420 388
270 166 108 78 77
Bpu10I CC'TnA_GC
Cuts at: 2526 2737 3102 3120 3575 3588 3972 4827 4941
Size: 211 365 18 455 13 384 855 114
Cuts at: 4941 5471 2526
Size: 530 5495
Fragments arranged by size:
5495 855 530 455 384 365 211 114
18 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4637 4865 5651 6565 7430 7728 4637
Size: 228 786 914 865 298 5349
Fragments arranged by size:
5349 914 865 786 298 228
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7103 882
Size: 617 5604 2219
Fragments arranged by size:
5604 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3770 4957 5737 6231 493
Size: 711 2566 1187 780 494 2702
Fragments arranged by size:
2702 2566 1187 780 711 494
BsaBI GATnn'nnATC
Cuts at: 3081 8434 3081
Size: 5353 3087
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4376 5050 6247 6629
Size: 53 83 186 936 2843 674 1197 382
Cuts at: 6629 7264 275
Size: 635 1451
Fragments arranged by size:
2843 1451 1197 936 674 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5364 6873 7271 7545 7692 8103 5364
Size: 1509 398 274 147 411 5701
Fragments arranged by size:
5701 1509 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2406 2436 2584 2614 4442
Size: 30 165 30 1003 30 148 30 1828
Cuts at: 4442 4472 1178
Size: 30 5146
Fragments arranged by size:
5146 1828 1003 165 148 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2439 2983 3937 4535 4540 4697 4754 4796
Size: 1253 544 954 598 5 157 57 42
Cuts at: 4796 4820 5300 1186
Size: 24 480 4326
Fragments arranged by size:
4326 1253 954 598 544 480 157 57
42 24 5
BseYI C'CCAG_C
Cuts at: 2198 2351 2795 3394 3593 4322 4461 4469 4578
Size: 153 444 599 199 729 139 8 109
Cuts at: 4578 4691 4817 5039 5424 5536 7643 8139 2198
Size: 113 126 222 385 112 2107 496 2499
Fragments arranged by size:
2499 2107 729 599 496 444 385 222
199 153 139 126 113 112 109 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2547 3769 4274 1163
Size: 573 811 1222 505 5329
Fragments arranged by size:
5329 1222 811 573 505
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5890 6651 7679 1540
Size: 275 186 3889 761 1028 2301
Fragments arranged by size:
3889 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3806 6501 6586 7657 3806
Size: 2695 85 1071 4589
Fragments arranged by size:
4589 2695 1071 85
BsmI GAATG_Cn'
Cuts at: 2394 5951 6044 2394
Size: 3557 93 4790
Fragments arranged by size:
4790 3557 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2328 4300 5497 2328
Size: 1972 1197 5271
Fragments arranged by size:
5271 1972 1197
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2344 2411 2465 2590 2805 2829 2837
Size: 399 554 67 54 125 215 24 8
Cuts at: 2837 2859 3360 3581 3792 3806 4145 4322 4406
Size: 22 501 221 211 14 339 177 84
Cuts at: 4406 4444 4604 4714 4833 5188 5239 5425 5530
Size: 38 160 110 119 355 51 186 105
Cuts at: 5530 5710 5759 6501 6586 7657 1391
Size: 180 49 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 554 501 399 355 339
221 215 211 186 180 177 160 125
119 110 105 85 84 67 54 51
49 38 24 22 14 8
BspEI T'CCGG_A
Cuts at: 8103 8103
Size: 8440
BspHI T'CATG_A
Cuts at: 3816 5394 6329 8059 3816
Size: 1578 935 1730 4197
Fragments arranged by size:
4197 1730 1578 935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3097 3558 3716 4157 5088 5795 844
Size: 2253 461 158 441 931 707 3489
Fragments arranged by size:
3489 2253 931 707 461 441 158
BsrBI CCG'CTC
Cuts at: 1394 1823 3991 6327 1394
Size: 429 2168 2336 3507
Fragments arranged by size:
3507 2336 2168 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2977 4141 6935 7109 65
Size: 1183 1729 1164 2794 174 1396
Fragments arranged by size:
2794 1729 1396 1183 1164 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3614 7084 7271 1700
Size: 110 1804 3470 187 2869
Fragments arranged by size:
3470 2869 1804 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8440
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8440
BssSI C'ACGA_G
Cuts at: 5069 6500 7512 5069
Size: 1431 1012 5997
Fragments arranged by size:
5997 1431 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2195 3717 4140 4573 4772 5103 5173 5646
Size: 1401 1522 423 433 199 331 70 473
Cuts at: 5646 794
Size: 3588
Fragments arranged by size:
3588 1522 1401 473 433 423 331 199
70
BstBI TT'CG_AA
Cuts at: 2007 7244 2007
Size: 5237 3203
BstEII G'GTnAC_C
Cuts at: 1184 3703 1184
Size: 2519 5921
BstXI CCAn_nnnn'nTGG
Cuts at: 2351 2636 3265 3514 4670 5195 5530 2351
Size: 285 629 249 1156 525 335 5261
Fragments arranged by size:
5261 1156 629 525 335 285 249
BstYI r'GATC_y
Cuts at: 1972 2545 3076 3531 5336 5770 6137 6522 6539
Size: 573 531 455 1805 434 367 385 17
Cuts at: 6539 7991 8077 8089 8435 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1805 1452 573 531 455 434 385
367 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2751 3372 4737 5045 2751
Size: 621 1365 308 6146
Fragments arranged by size:
6146 1365 621 308
BtgI C'CryG_G
Cuts at: 513 1065 2022 2236 2757 4416 5347 513
Size: 552 957 214 521 1659 931 3606
Fragments arranged by size:
3606 1659 957 931 552 521 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7701
Fragments arranged by size:
7701 683 56
BtsI GCAGTG_nn'
Cuts at: 3715 3806 4381 5287 5435 5956 6750 6770 3715
Size: 91 575 906 148 521 794 20 5385
Fragments arranged by size:
5385 906 794 575 521 148 91 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8440
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8405
DraI TTT'AAA
Cuts at: 5744 5744
Size: 8440
DraIII CAC_nnn'GTG
Cuts at: 2292 2292
Size: 8440
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7447 809
Size: 507 6131 1802
Fragments arranged by size:
6131 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3231 4017 5197
Size: 54 1425 50 275 18 1401 786 1180
Cuts at: 5197 5887 6776 8405 8
Size: 690 889 1629 43
Fragments arranged by size:
1629 1425 1401 1180 889 786 690 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5887 1537
Size: 275 4075 4090
Fragments arranged by size:
4090 4075 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2617 3653 4797 5495 5547 6370 1353
Size: 1264 1036 1144 698 52 823 3423
Fragments arranged by size:
3423 1264 1144 1036 823 698 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5023 5343 7027 7401 7547 387
Size: 1064 138 3434 320 1684 374 146 1280
Fragments arranged by size:
3434 1684 1280 1064 374 320 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2196 2232 2340 2728 2805 2971
Size: 393 522 42 36 108 388 77 166
Cuts at: 2971 3650 4070 4165 4782 4923 5110 5361 5631
Size: 679 420 95 617 141 187 251 270
Cuts at: 5631 6503 7100 7887 8155 1239
Size: 872 597 787 268 1524
Fragments arranged by size:
1524 872 787 679 617 597 522 420
393 388 270 268 251 187 166 141
108 95 77 42 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2529 3371 3515 2529
Size: 842 144 7454
Fragments arranged by size:
7454 842 144
EcoO109I rG'GnC_Cy
Cuts at: 2129 2311 2340 2369 2378 2679 2719 3067 3108
Size: 182 29 29 9 301 40 348 41
Cuts at: 3108 3976 4898 5184 5557 2129
Size: 868 922 286 373 5012
Fragments arranged by size:
5012 922 868 373 348 301 286 182
41 40 29 29 9
EcoRI G'AATT_C
Cuts at: 5749 6127 5749
Size: 378 8062
EcoRV GAT'ATC
Cuts at: 3492 3492
Size: 8440
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5692 5692
Size: 8440
FspI TGC'GCA
Cuts at: 3695 6946 3695
Size: 3251 5189
FspAI rTGC'GCAy
Cuts at: 3695 3695
Size: 8440
HaeII r_GCGC'y
Cuts at: 1678 4188 4678 5023 5053 7587 1678
Size: 2510 490 345 30 2534 2531
Fragments arranged by size:
2534 2531 2510 490 345 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2584 2616 3046 3078 3632
Size: 32 135 32 1012 32 430 32 554
Cuts at: 3632 3664 4772 4804 7081 7113 7155 7187 1373
Size: 32 1108 32 2277 32 42 32 2626
Fragments arranged by size:
2626 2277 1108 1012 554 430 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5784 6030 669
Size: 988 281 3846 246 3079
Fragments arranged by size:
3846 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8440
HpaI GTT'AAC
Cuts at: 6030 6030
Size: 8440
KasI G'GCGC_C
Cuts at: 5049 5049
Size: 8440
KpnI G_GTAC'C
Cuts at: 5765 5765
Size: 8440
MfeI C'AATT_G
Cuts at: 6039 6039
Size: 8440
MluI A'CGCG_T
Cuts at: 8316 8316
Size: 8440
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3665 5231 7554 7738 1305
Size: 2360 1566 2323 184 2007
Fragments arranged by size:
2360 2323 2007 1566 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3233 10
Size: 54 1425 343 1401 5217
Fragments arranged by size:
5217 1425 1401 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3263 3768 6398 6757 6916 518
Size: 648 2097 505 2630 359 159 2042
Fragments arranged by size:
2630 2097 2042 648 505 359 159
MspA1I CmG'CkG
Cuts at: 1606 2551 3587 3624 3868 4326 5856 6533 7681
Size: 945 1036 37 244 458 1530 677 1148
Cuts at: 7681 7926 1606
Size: 245 2120
Fragments arranged by size:
2120 1530 1148 1036 945 677 458 245
244 37
NarI GG'CG_CC
Cuts at: 5050 5050
Size: 8440
NcoI C'CATG_G
Cuts at: 513 1065 2022 2236 4416 5347 513
Size: 552 957 214 2180 931 3606
Fragments arranged by size:
3606 2180 957 931 552 214
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8440
NheI G'CTAG_C
Cuts at: 1052 3125 1052
Size: 2073 6367
NotI GC'GGCC_GC
Cuts at: 5887 5887
Size: 8440
NsiI A_TGCA'T
Cuts at: 4203 4203
Size: 8440
NspI r_CATG'y
Cuts at: 1132 1229 2650 3694 3767 4038 4201 5156 5798
Size: 97 1421 1044 73 271 163 955 642
Cuts at: 5798 7343 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1421 1044 955 642 271 163
97 73
PasI CC'CwG_GG
Cuts at: 1348 2141 4121 5239 5322 5530 1348
Size: 793 1980 1118 83 208 4258
Fragments arranged by size:
4258 1980 1118 793 208 83
PciI A'CATG_T
Cuts at: 5152 7339 5152
Size: 2187 6253
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2795 3844 1108
Size: 1687 1049 5704
Fragments arranged by size:
5704 1687 1049
PfoI T'CCnGG_A
Cuts at: 4275 4275
Size: 8440
PmeI GTTT'AAAC
Cuts at: 5744 5744
Size: 8440
PmlI CAC'GTG
Cuts at: 3770 3770
Size: 8440
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2406 2438 5376 5408 6509 6541 8022 8054 2406
Size: 32 2938 32 1101 32 1481 32 2792
Fragments arranged by size:
2938 2792 1481 1101 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2719 3067 2719
Size: 348 8092
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8440
PsiI TTA'TAA
Cuts at: 6010 6010
Size: 8440
PspOMI G'GGCC_C
Cuts at: 2340 5184 2340
Size: 2844 5596
PstI C_TGCA'G
Cuts at: 830 2702 2959 4178 5099 5792 830
Size: 1872 257 1219 921 693 3478
Fragments arranged by size:
3478 1872 1219 921 693 257
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8440
PvuII CAG'CTG
Cuts at: 3587 4326 3587
Size: 739 7701
SalI G'TCGA_C
Cuts at: 1936 5782 1936
Size: 3846 4594
SapI GCTCTTCn'nnn_
Cuts at: 3653 3653
Size: 8440
SbfI CC_TGCA'GG
Cuts at: 5099 5792 5099
Size: 693 7747
ScaI AGT'ACT
Cuts at: 3253 6688 8400 3253
Size: 3435 1712 3293
Fragments arranged by size:
3435 3293 1712
SexAI A'CCwGG_T
Cuts at: 2155 2155
Size: 8440
SfcI C'TryA_G
Cuts at: 826 1045 2262 2698 2955 3562 3825 3876 3960
Size: 219 1217 436 257 607 263 51 84
Cuts at: 3960 4174 5095 5788 6923 7604 7795 826
Size: 214 921 693 1135 681 191 1471
Fragments arranged by size:
1471 1217 1135 921 693 681 607 436
263 257 219 214 191 84 51
SfiI GGCCn_nnn'nGGCC
Cuts at: 2377 2377
Size: 8440
SfoI GGC'GCC
Cuts at: 5051 5051
Size: 8440
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8440
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8440
SmaI CCC'GGG
Cuts at: 1872 5767 1872
Size: 3895 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3005 4652 4880 5666 6544 7445
Size: 197 926 1062 1647 228 786 878 901
Cuts at: 7445 7707 820
Size: 262 1553
Fragments arranged by size:
1647 1553 1062 926 901 878 786 262
228 197
SnaBI TAC'GTA
Cuts at: 493 5737 6231 493
Size: 5244 494 2702
Fragments arranged by size:
5244 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8440
SphI G_CATG'C
Cuts at: 1132 2650 3694 3767 4201 5798 1132
Size: 1518 1044 73 434 1597 3774
Fragments arranged by size:
3774 1597 1518 1044 434 73
SspI AAT'ATT
Cuts at: 5 52 6364 5
Size: 47 6312 2081
Fragments arranged by size:
6312 2081 47
StuI AGG'CCT
Cuts at: 1517 2228 3001 3351 3521 3823 4283 4878 1517
Size: 711 773 350 170 302 460 595 5079
Fragments arranged by size:
5079 773 711 595 460 350 302 170
StyI C'CwwG_G
Cuts at: 513 1065 2022 2236 2499 3096 3918 4416 5211
Size: 552 957 214 263 597 822 498 795
Cuts at: 5211 5347 5879 513
Size: 136 532 3074
Fragments arranged by size:
3074 957 822 795 597 552 532 498
263 214 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3182 4676 6467 6484 6637 6822 7267
Size: 1255 1602 1494 1791 17 153 185 445
Cuts at: 7267 325
Size: 1498
Fragments arranged by size:
1791 1602 1498 1494 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3251 3470 6686
Size: 274 80 33 51 1443 1274 219 3216
Cuts at: 6686 8398 96
Size: 1712 138
Fragments arranged by size:
3216 1712 1443 1274 274 219 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2278 2310 3399 3431 4442 4474 8400 8432 2278
Size: 32 1089 32 1011 32 3926 32 2286
Fragments arranged by size:
3926 2286 1089 1011 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3502 4523 1316
Size: 2186 1021 5233
Fragments arranged by size:
5233 2186 1021
XbaI T'CTAG_A
Cuts at: 5776 5776
Size: 8440
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4330 4414 4330
Size: 84 8356
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8440
XmaI C'CCGG_G
Cuts at: 1870 5765 1870
Size: 3895 4545
XmnI GAAnn'nnTTC
Cuts at: 4997 6569 4997
Size: 1572 6868
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4377 6248 276
Size: 53 83 186 936 2843 1871 2468
Fragments arranged by size:
2843 2468 1871 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BplI BsiWI BstZ17I EcoICRI FseI
NaeI NgoMIV NruI PacI PspXI PsrI RsrII SacI
SacII SanDI SrfI SwaI