Restriction map for
pFN21ASDA1441
(Linear) (Six-base) MAPSORT of: KIAA1441.seq Check: 8602 from: 1 to: 3711
KIAA1441.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1074 1693 3711
Size: 1074 619 2018
Fragments arranged by size:
2018 1074 619
AatII G_ACGT'C
Cuts at: 0 2356 3711
Size: 2356 1355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 2142 2759 3087 3711
Size: 173 1969 617 328 624
Fragments arranged by size:
1969 624 617 328 173
AfeI AGC'GCT
Cuts at: 0 2998 3711
Size: 2998 713
AflII C'TTAA_G
Cuts at: 0 982 3711
Size: 982 2729
AflIII A'CryG_T
Cuts at: 0 3129 3711
Size: 3129 582
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1216 1543 2242 3711
Size: 1216 327 699 1469
Fragments arranged by size:
1469 1216 699 327
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 383 415 3711
Size: 383 32 3296
Fragments arranged by size:
3296 383 32
AlwNI CAG_nnn'CTG
Cuts at: 0 172 772 1057 1438 1945 2749 3711
Size: 172 600 285 381 507 804 962
Fragments arranged by size:
962 804 600 507 381 285 172
ApaI G_GGCC'C
Cuts at: 0 321 3165 3711
Size: 321 2844 546
Fragments arranged by size:
2844 546 321
AvaI C'yCGr_G
Cuts at: 0 274 554 3194 3711
Size: 274 280 2640 517
Fragments arranged by size:
2640 517 280 274
BanI G'GyrC_C
Cuts at: 0 439 777 803 1332 1399 2294 2686 3026
Size: 439 338 26 529 67 895 392 340
Cuts at: 3026 3397 3711
Size: 371 314
Fragments arranged by size:
895 529 439 392 371 340 338 314
67 26
BanII G_rGCy'C
Cuts at: 0 321 388 567 814 1558 1769 2810 3165
Size: 321 67 179 247 744 211 1041 355
Cuts at: 3165 3216 3687 3711
Size: 51 471 24
Fragments arranged by size:
1041 744 471 355 321 247 211 179
67 51 24
BbeI G_GCGC'C
Cuts at: 0 3030 3711
Size: 3030 681
BbsI GAAGACnn'nnnn_
Cuts at: 0 19 161 881 1704 2496 2759 3236 3711
Size: 19 142 720 823 792 263 477 475
Fragments arranged by size:
823 792 720 477 475 263 142 19
BbvCI CC'TCA_GC
Cuts at: 0 1079 1097 1565 1949 3711
Size: 1079 18 468 384 1762
Fragments arranged by size:
1762 1079 468 384 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1656 1690 2167 2201 2257 2291 3711
Size: 1656 34 477 34 56 34 1420
Fragments arranged by size:
1656 1420 477 56 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2281 3711
Size: 2281 1430
BfrBI ATG'CAT
Cuts at: 0 2178 3711
Size: 2178 1533
BglI GCCn_nnn'nGGC
Cuts at: 0 354 1075 1093 1255 2101 3711
Size: 354 721 18 162 846 1610
Fragments arranged by size:
1610 846 721 354 162 18
BlpI GC'TnA_GC
Cuts at: 0 303 3711
Size: 303 3408
Bme1580I G_kGCm'C
Cuts at: 0 321 442 782 806 836 1337 2122 2299
Size: 321 121 340 24 30 501 785 177
Cuts at: 2299 2383 2421 2581 2691 3165 3402 3507 3711
Size: 84 38 160 110 474 237 105 204
Fragments arranged by size:
785 501 474 340 321 237 204 177
160 121 110 105 84 38 30 24
BmgBI CAC'GTC
Cuts at: 0 2271 3711
Size: 2271 1440
BmrI ACTGGGnnnn_n'
Cuts at: 0 425 760 1388 1448 1473 3159 3711
Size: 425 335 628 60 25 1686 552
Fragments arranged by size:
1686 628 552 425 335 60 25
BmtI G_CTAG'C
Cuts at: 0 1106 3711
Size: 1106 2605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 209 317 705 782 948 1627 2047
Size: 131 78 108 388 77 166 679 420
Cuts at: 2047 2900 3338 3608 3711
Size: 853 438 270 103
Fragments arranged by size:
853 679 438 420 388 270 166 131
108 103 78 77
Bpu10I CC'TnA_GC
Cuts at: 0 503 714 1079 1097 1552 1565 1949 2804
Size: 503 211 365 18 455 13 384 855
Cuts at: 2804 2918 3448 3711
Size: 114 530 263
Fragments arranged by size:
855 530 503 455 384 365 263 211
114 18 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2614 2842 3628 3711
Size: 2614 228 786 83
Fragments arranged by size:
2614 786 228 83
BsaAI yAC'GTr
Cuts at: 0 1747 2934 3711
Size: 1747 1187 777
Fragments arranged by size:
1747 1187 777
BsaBI GATnn'nnATC
Cuts at: 0 1058 3711
Size: 1058 2653
BsaHI Gr'CG_yC
Cuts at: 0 2353 3027 3711
Size: 2353 674 684
Fragments arranged by size:
2353 684 674
BsaWI w'CCGG_w
Cuts at: 0 3341 3711
Size: 3341 370
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 383 413 561 591 2419 2449 3711
Size: 383 30 148 30 1828 30 1262
Fragments arranged by size:
1828 1262 383 148 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 416 960 1914 2512 2517 2674 2731 2773
Size: 416 544 954 598 5 157 57 42
Cuts at: 2773 2797 3277 3711
Size: 24 480 434
Fragments arranged by size:
954 598 544 480 434 416 157 57
42 24 5
BseYI C'CCAG_C
Cuts at: 0 175 328 772 1371 1570 2299 2438 2446
Size: 175 153 444 599 199 729 139 8
Cuts at: 2446 2555 2668 2794 3016 3401 3513 3711
Size: 109 113 126 222 385 112 198
Fragments arranged by size:
729 599 444 385 222 199 198 175
153 139 126 113 112 109 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 1746 2251 3711
Size: 524 1222 505 1460
Fragments arranged by size:
1460 1222 524 505
BsiHKAI G_wGCw'C
Cuts at: 0 1783 3711
Size: 1783 1928
BsmI GAATG_Cn'
Cuts at: 0 371 3711
Size: 371 3340
BsmBI CGTCTCn'nnnn_
Cuts at: 0 305 2277 3474 3711
Size: 305 1972 1197 237
Fragments arranged by size:
1972 1197 305 237
Bsp1286I G_dGCh'C
Cuts at: 0 321 388 442 567 782 806 814 836
Size: 321 67 54 125 215 24 8 22
Cuts at: 836 1337 1558 1769 1783 2122 2299 2383 2421
Size: 501 221 211 14 339 177 84 38
Cuts at: 2421 2581 2691 2810 3165 3216 3402 3507 3687
Size: 160 110 119 355 51 186 105 180
Cuts at: 3687 3711
Size: 24
Fragments arranged by size:
501 355 339 321 221 215 211 186
180 177 160 125 119 110 105 84
67 54 51 38 24 24 22 14
8
BspHI T'CATG_A
Cuts at: 0 1793 3371 3711
Size: 1793 1578 340
Fragments arranged by size:
1793 1578 340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1074 1535 1693 2134 3065 3711
Size: 1074 461 158 441 931 646
Fragments arranged by size:
1074 931 646 461 441 158
BsrBI CCG'CTC
Cuts at: 0 1968 3711
Size: 1968 1743
BsrDI GCAATG_nn'
Cuts at: 0 954 2118 3711
Size: 954 1164 1593
Fragments arranged by size:
1593 1164 954
BsrFI r'CCGG_y
Cuts at: 0 1591 3711
Size: 1591 2120
BssSI C'ACGA_G
Cuts at: 0 3046 3711
Size: 3046 665
BstAPI GCAn_nnn'nTGC
Cuts at: 0 172 1694 2117 2550 2749 3080 3150 3623
Size: 172 1522 423 433 199 331 70 473
Cuts at: 3623 3711
Size: 88
Fragments arranged by size:
1522 473 433 423 331 199 172 88
70
BstEII G'GTnAC_C
Cuts at: 0 1680 3711
Size: 1680 2031
BstXI CCAn_nnnn'nTGG
Cuts at: 0 328 613 1242 1491 2647 3172 3507 3711
Size: 328 285 629 249 1156 525 335 204
Fragments arranged by size:
1156 629 525 335 328 285 249 204
BstYI r'GATC_y
Cuts at: 0 522 1053 1508 3313 3711
Size: 522 531 455 1805 398
Fragments arranged by size:
1805 531 522 455 398
Bsu36I CC'TnA_GG
Cuts at: 0 728 1349 2714 3022 3711
Size: 728 621 1365 308 689
Fragments arranged by size:
1365 728 689 621 308
BtgI C'CryG_G
Cuts at: 0 213 734 2393 3324 3711
Size: 213 521 1659 931 387
Fragments arranged by size:
1659 931 521 387 213
BtsI GCAGTG_nn'
Cuts at: 0 1692 1783 2358 3264 3412 3711
Size: 1692 91 575 906 148 299
Fragments arranged by size:
1692 906 575 299 148 91
DraIII CAC_nnn'GTG
Cuts at: 0 269 3711
Size: 269 3442
EaeI y'GGCC_r
Cuts at: 0 1208 1994 3174 3711
Size: 1208 786 1180 537
Fragments arranged by size:
1208 1180 786 537
EarI CTCTTCn'nnn_
Cuts at: 0 594 1630 2774 3472 3524 3711
Size: 594 1036 1144 698 52 187
Fragments arranged by size:
1144 1036 698 594 187 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3000 3320 3711
Size: 3000 320 391
Fragments arranged by size:
3000 391 320
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 173 209 317 705 782 948 1627
Size: 131 42 36 108 388 77 166 679
Cuts at: 1627 2047 2142 2759 2900 3087 3338 3608 3711
Size: 420 95 617 141 187 251 270 103
Fragments arranged by size:
679 617 420 388 270 251 187 166
141 131 108 103 95 77 42 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 506 1348 1492 3711
Size: 506 842 144 2219
Fragments arranged by size:
2219 842 506 144
EcoO109I rG'GnC_Cy
Cuts at: 0 106 288 317 346 355 656 696 1044
Size: 106 182 29 29 9 301 40 348
Cuts at: 1044 1085 1953 2875 3161 3534 3711
Size: 41 868 922 286 373 177
Fragments arranged by size:
922 868 373 348 301 286 182 177
106 41 40 29 29 9
EcoRV GAT'ATC
Cuts at: 0 1469 3711
Size: 1469 2242
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3669 3711
Size: 3669 42
FspI TGC'GCA
Cuts at: 0 1672 3711
Size: 1672 2039
FspAI rTGC'GCAy
Cuts at: 0 1672 3711
Size: 1672 2039
HaeII r_GCGC'y
Cuts at: 0 2165 2655 3000 3030 3711
Size: 2165 490 345 30 681
Fragments arranged by size:
2165 681 490 345 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 561 593 1023 1055 1609 1641 2749 2781
Size: 561 32 430 32 554 32 1108 32
Cuts at: 2781 3711
Size: 930
Fragments arranged by size:
1108 930 561 554 430 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 3026 3711
Size: 3026 685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1642 3208 3711
Size: 1642 1566 503
Fragments arranged by size:
1642 1566 503
MscI TGG'CCA
Cuts at: 0 1210 3711
Size: 1210 2501
MslI CAynn'nnrTG
Cuts at: 0 1240 1745 3711
Size: 1240 505 1966
Fragments arranged by size:
1966 1240 505
MspA1I CmG'CkG
Cuts at: 0 528 1564 1601 1845 2303 3711
Size: 528 1036 37 244 458 1408
Fragments arranged by size:
1408 1036 528 458 244 37
NarI GG'CG_CC
Cuts at: 0 3027 3711
Size: 3027 684
NcoI C'CATG_G
Cuts at: 0 213 2393 3324 3711
Size: 213 2180 931 387
Fragments arranged by size:
2180 931 387 213
NheI G'CTAG_C
Cuts at: 0 1102 3711
Size: 1102 2609
NsiI A_TGCA'T
Cuts at: 0 2180 3711
Size: 2180 1531
NspI r_CATG'y
Cuts at: 0 627 1671 1744 2015 2178 3133 3711
Size: 627 1044 73 271 163 955 578
Fragments arranged by size:
1044 955 627 578 271 163 73
PasI CC'CwG_GG
Cuts at: 0 118 2098 3216 3299 3507 3711
Size: 118 1980 1118 83 208 204
Fragments arranged by size:
1980 1118 208 204 118 83
PciI A'CATG_T
Cuts at: 0 3129 3711
Size: 3129 582
PflMI CCAn_nnn'nTGG
Cuts at: 0 772 1821 3711
Size: 772 1049 1890
Fragments arranged by size:
1890 1049 772
PfoI T'CCnGG_A
Cuts at: 0 2252 3711
Size: 2252 1459
PmlI CAC'GTG
Cuts at: 0 1747 3711
Size: 1747 1964
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 383 415 3353 3385 3711
Size: 383 32 2938 32 326
Fragments arranged by size:
2938 383 326 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 696 1044 3711
Size: 696 348 2667
Fragments arranged by size:
2667 696 348
PspOMI G'GGCC_C
Cuts at: 0 317 3161 3711
Size: 317 2844 550
Fragments arranged by size:
2844 550 317
PstI C_TGCA'G
Cuts at: 0 679 936 2155 3076 3711
Size: 679 257 1219 921 635
Fragments arranged by size:
1219 921 679 635 257
PvuII CAG'CTG
Cuts at: 0 1564 2303 3711
Size: 1564 739 1408
Fragments arranged by size:
1564 1408 739
SapI GCTCTTCn'nnn_
Cuts at: 0 1630 3711
Size: 1630 2081
SbfI CC_TGCA'GG
Cuts at: 0 3076 3711
Size: 3076 635
ScaI AGT'ACT
Cuts at: 0 1230 3711
Size: 1230 2481
SexAI A'CCwGG_T
Cuts at: 0 132 3711
Size: 132 3579
SfcI C'TryA_G
Cuts at: 0 239 675 932 1539 1802 1853 1937 2151
Size: 239 436 257 607 263 51 84 214
Cuts at: 2151 3072 3711
Size: 921 639
Fragments arranged by size:
921 639 607 436 263 257 239 214
84 51
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 354 3711
Size: 354 3357
SfoI GGC'GCC
Cuts at: 0 3028 3711
Size: 3028 683
SmlI C'TyrA_G
Cuts at: 0 982 2629 2857 3643 3711
Size: 982 1647 228 786 68
Fragments arranged by size:
1647 982 786 228 68
SphI G_CATG'C
Cuts at: 0 627 1671 1744 2178 3711
Size: 627 1044 73 434 1533
Fragments arranged by size:
1533 1044 627 434 73
StuI AGG'CCT
Cuts at: 0 205 978 1328 1498 1800 2260 2855 3711
Size: 205 773 350 170 302 460 595 856
Fragments arranged by size:
856 773 595 460 350 302 205 170
StyI C'CwwG_G
Cuts at: 0 213 476 1073 1895 2393 3188 3324 3711
Size: 213 263 597 822 498 795 136 387
Fragments arranged by size:
822 795 597 498 387 263 213 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1159 2653 3711
Size: 1159 1494 1058
Fragments arranged by size:
1494 1159 1058
TatI w'GTAC_w
Cuts at: 0 1228 1447 3711
Size: 1228 219 2264
Fragments arranged by size:
2264 1228 219
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 255 287 1376 1408 2419 2451 3711
Size: 255 32 1089 32 1011 32 1260
Fragments arranged by size:
1260 1089 1011 255 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1479 2500 3711
Size: 1479 1021 1211
Fragments arranged by size:
1479 1211 1021
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2307 2391 3711
Size: 2307 84 1320
Fragments arranged by size:
2307 1320 84
XmnI GAAnn'nnTTC
Cuts at: 0 2974 3711
Size: 2974 737
ZraI GAC'GTC
Cuts at: 0 2354 3711
Size: 2354 1357
Enzymes that do cut:
AarI AatII AcuI AfeI AflII AflIII AlfI AloI
AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRV FalI FspI FspAI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI
NcoI NheI NsiI NspI PasI PciI PflMI PfoI
PmlI PpiI PpuMI PspOMI PstI PvuII SapI SbfI
ScaI SexAI SfcI SfiI SfoI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AgeI AhdI AleI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BclI BglII
BplI BsaI BsiEI BsiWI BspEI BsrGI BssHII BstBI
BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI
EcoRI FseI HincII HindIII HpaI KpnI MfeI MluI
NaeI NdeI NgoMIV NotI NruI PacI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI XbaI XhoI XmaI