(Circular) (Six-base) MAPSORT of: pFN21ASDA1439.seq Check: 8662 from: 1 to: 6256 pFN21ASDA1439 6256 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4066 278 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3599 1937 Size: 1662 4594 Acc65I G'GTAC_C Cuts at: 3540 3577 3540 Size: 37 6219 AclI AA'CG_TT Cuts at: 1563 4383 4756 1563 Size: 2820 373 3063 Fragments arranged by size: 3063 2820 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3011 4319 5703 5971 1632 Size: 1379 1308 1384 268 1917 Fragments arranged by size: 1917 1384 1379 1308 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6059 AflIII A'CryG_T Cuts at: 1203 5155 6132 1203 Size: 3952 977 1327 Fragments arranged by size: 3952 1327 977 AgeI A'CCGG_T Cuts at: 5087 5087 Size: 6256 AhdI GACnn_n'nnGTC Cuts at: 4985 4985 Size: 6256 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6256 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6256 AlwNI CAG_nnn'CTG Cuts at: 2741 3999 5571 2741 Size: 1258 1572 3426 Fragments arranged by size: 3426 1572 1258 ApaLI G'TGCA_C Cuts at: 3302 4313 5469 3302 Size: 1011 1156 4089 Fragments arranged by size: 4089 1156 1011 ApoI r'AATT_y Cuts at: 1493 2054 2749 3215 3565 3791 3943 6052 1493 Size: 561 695 466 350 226 152 2109 1697 Fragments arranged by size: 2109 1697 695 561 466 350 226 152 AseI AT'TA_AT Cuts at: 160 1032 4810 160 Size: 872 3778 1606 Fragments arranged by size: 3778 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 AvaI C'yCGr_G Cuts at: 1870 1943 3581 1870 Size: 73 1638 4545 Fragments arranged by size: 4545 1638 73 BamHI G'GATC_C Cuts at: 3499 3586 3953 3499 Size: 87 367 5802 Fragments arranged by size: 5802 367 87 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3379 3540 3577 5032 618 Size: 325 217 627 1592 161 37 1455 1842 Fragments arranged by size: 1842 1592 1455 627 325 217 161 37 BanII G_rGCy'C Cuts at: 1391 2505 2843 3575 1391 Size: 1114 338 732 4072 Fragments arranged by size: 4072 1114 732 338 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5285 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3099 3133 4447 4481 1903 Size: 34 1162 34 1314 34 3678 Fragments arranged by size: 3678 1314 1162 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3542 4148 5358 3542 Size: 606 1210 4440 Fragments arranged by size: 4440 1210 606 BclI T'GATC_A Cuts at: 1548 1548 Size: 6256 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4867 136 Size: 107 122 71 631 605 3195 1525 Fragments arranged by size: 3195 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6251 6251 Size: 6256 BlpI GC'TnA_GC Cuts at: 3673 3673 Size: 6256 Bme1580I G_kGCm'C Cuts at: 1790 3306 4317 5473 1790 Size: 1516 1011 1156 2573 Fragments arranged by size: 2573 1516 1156 1011 BmgBI CAC'GTC Cuts at: 1318 2089 3405 3435 1318 Size: 771 1316 30 4139 Fragments arranged by size: 4139 1316 771 30 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3987 4945 442 Size: 416 53 83 399 24 2570 958 1753 Fragments arranged by size: 2570 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6256 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2422 4916 1239 Size: 1183 2494 2579 Fragments arranged by size: 2579 2494 1183 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2840 4381 5246 5544 2840 Size: 1541 865 298 3552 Fragments arranged by size: 3552 1541 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4919 882 Size: 617 3420 2219 Fragments arranged by size: 3420 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3553 4047 493 Size: 711 2349 494 2702 Fragments arranged by size: 2702 2349 711 494 BsaBI GATnn'nnATC Cuts at: 6250 6250 Size: 6256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4063 4445 5080 275 Size: 53 83 186 936 2530 382 635 1451 Fragments arranged by size: 2530 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4689 5087 5361 5508 5919 4689 Size: 398 274 147 411 5026 Fragments arranged by size: 5026 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3379 3409 1178 Size: 30 165 30 1976 30 4025 Fragments arranged by size: 4025 1976 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2824 2906 3031 1186 Size: 1638 82 125 4411 Fragments arranged by size: 4411 1638 125 82 BseYI C'CCAG_C Cuts at: 2603 2822 3178 3455 5459 5955 2603 Size: 219 356 277 2004 496 2904 Fragments arranged by size: 2904 2004 496 356 277 219 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3441 1163 Size: 573 1705 3978 Fragments arranged by size: 3978 1705 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3706 4467 5495 1540 Size: 275 186 1705 761 1028 2301 Fragments arranged by size: 2301 1705 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2843 3306 4317 4402 5473 2843 Size: 463 1011 85 1071 3626 Fragments arranged by size: 3626 1071 1011 463 85 BsmI GAATG_Cn' Cuts at: 2976 3767 3860 2976 Size: 791 93 5372 Fragments arranged by size: 5372 791 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3197 3197 Size: 6256 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2505 2843 3306 3575 4317 4402 5473 Size: 399 715 338 463 269 742 85 1071 Cuts at: 5473 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 715 463 399 338 269 85 BspEI T'CCGG_A Cuts at: 5919 5919 Size: 6256 BspHI T'CATG_A Cuts at: 4145 5875 4145 Size: 1730 4526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2127 3611 844 Size: 1283 1484 3489 Fragments arranged by size: 3489 1484 1283 BsrBI CCG'CTC Cuts at: 1394 1823 2035 4143 1394 Size: 429 212 2108 3507 Fragments arranged by size: 3507 2108 429 212 BsrDI GCAATG_nn' Cuts at: 65 1248 2811 4751 4925 65 Size: 1183 1563 1940 174 1396 Fragments arranged by size: 1940 1563 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3376 4900 5087 1700 Size: 110 1566 1524 187 2869 Fragments arranged by size: 2869 1566 1524 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6256 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6256 BssSI C'ACGA_G Cuts at: 4316 5328 4316 Size: 1012 5244 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6256 BstBI TT'CG_AA Cuts at: 2007 5060 2007 Size: 3053 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6256 BstXI CCAn_nnnn'nTGG Cuts at: 3158 3158 Size: 6256 BstYI r'GATC_y Cuts at: 1972 3499 3586 3953 4338 4355 5807 5893 5905 Size: 1527 87 367 385 17 1452 86 12 Cuts at: 5905 6251 1972 Size: 346 1977 Fragments arranged by size: 1977 1527 1452 385 367 346 87 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3198 3198 Size: 6256 BtgI C'CryG_G Cuts at: 513 1065 3494 513 Size: 552 2429 3275 Fragments arranged by size: 3275 2429 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5517 Fragments arranged by size: 5517 683 56 BtsI GCAGTG_nn' Cuts at: 3772 4566 4586 3772 Size: 794 20 5442 Fragments arranged by size: 5442 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6221 DraI TTT'AAA Cuts at: 2434 3560 2434 Size: 1126 5130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2672 5263 809 Size: 507 1356 2591 1802 Fragments arranged by size: 2591 1802 1356 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3357 3703 4592 Size: 54 1425 50 275 18 1527 346 889 Cuts at: 4592 6221 8 Size: 1629 43 Fragments arranged by size: 1629 1527 1425 889 346 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3703 1537 Size: 275 1891 4090 Fragments arranged by size: 4090 1891 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2172 3129 4186 1353 Size: 819 957 1057 3423 Fragments arranged by size: 3423 1057 957 819 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2372 4843 5217 5363 387 Size: 1064 138 783 2471 374 146 1280 Fragments arranged by size: 2471 1280 1064 783 374 146 138 EcoICRI GAG'CTC Cuts at: 2841 2841 Size: 6256 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2422 3011 4319 4916 5703 5971 1239 Size: 393 790 589 1308 597 787 268 1524 Fragments arranged by size: 1524 1308 790 787 597 589 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2384 2384 Size: 6256 EcoO109I rG'GnC_Cy Cuts at: 3089 3089 Size: 6256 EcoRI G'AATT_C Cuts at: 3565 3943 3565 Size: 378 5878 EcoRV GAT'ATC Cuts at: 2269 2269 Size: 6256 FspI TGC'GCA Cuts at: 4762 4762 Size: 6256 HaeII r_GCGC'y Cuts at: 1678 2470 2854 2920 5403 1678 Size: 792 384 66 2483 2531 Fragments arranged by size: 2531 2483 792 384 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4897 4929 4971 5003 1373 Size: 32 135 32 3325 32 42 32 2626 Fragments arranged by size: 3325 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3408 3600 3846 669 Size: 988 281 1470 192 246 3079 Fragments arranged by size: 3079 1470 988 281 246 192 HindIII A'AGCT_T Cuts at: 748 3505 748 Size: 2757 3499 HpaI GTT'AAC Cuts at: 3846 3846 Size: 6256 KpnI G_GTAC'C Cuts at: 3544 3581 3544 Size: 37 6219 MfeI C'AATT_G Cuts at: 3855 3855 Size: 6256 MluI A'CGCG_T Cuts at: 6132 6132 Size: 6256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2296 2364 2388 2536 2979 3005 3247 3422 Size: 991 68 24 148 443 26 242 175 Cuts at: 3422 3469 5370 5554 1305 Size: 47 1901 184 2007 Fragments arranged by size: 2007 1901 991 443 242 184 175 148 68 47 26 24 MscI TGG'CCA Cuts at: 10 64 1489 1832 3359 10 Size: 54 1425 343 1527 2907 Fragments arranged by size: 2907 1527 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3326 4214 4573 4732 518 Size: 648 2160 888 359 159 2042 Fragments arranged by size: 2160 2042 888 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2446 3672 4349 5497 5742 1606 Size: 840 1226 677 1148 245 2120 Fragments arranged by size: 2120 1226 1148 840 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5704 NdeI CA'TA_TG Cuts at: 387 387 Size: 6256 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6256 NotI GC'GGCC_GC Cuts at: 3703 3703 Size: 6256 NspI r_CATG'y Cuts at: 1132 1229 2576 3110 3614 5159 1132 Size: 97 1347 534 504 1545 2229 Fragments arranged by size: 2229 1545 1347 534 504 97 PasI CC'CwG_GG Cuts at: 1348 3046 1348 Size: 1698 4558 PciI A'CATG_T Cuts at: 5155 5155 Size: 6256 PflMI CCAn_nnn'nTGG Cuts at: 1108 2645 2930 1108 Size: 1537 285 4434 Fragments arranged by size: 4434 1537 285 PfoI T'CCnGG_A Cuts at: 2661 2797 2661 Size: 136 6120 PmeI GTTT'AAAC Cuts at: 3560 3560 Size: 6256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4325 4357 5838 5870 4325 Size: 32 1481 32 4711 Fragments arranged by size: 4711 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3089 3089 Size: 6256 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6256 PsiI TTA'TAA Cuts at: 3826 3826 Size: 6256 PstI C_TGCA'G Cuts at: 830 2124 2737 3608 830 Size: 1294 613 871 3478 Fragments arranged by size: 3478 1294 871 613 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6256 SacI G_AGCT'C Cuts at: 2843 2843 Size: 6256 SalI G'TCGA_C Cuts at: 1936 3598 1936 Size: 1662 4594 SbfI CC_TGCA'GG Cuts at: 2124 3608 2124 Size: 1484 4772 ScaI AGT'ACT Cuts at: 2455 4504 6216 2455 Size: 2049 1712 2495 Fragments arranged by size: 2495 2049 1712 SfcI C'TryA_G Cuts at: 826 1045 2120 2733 2785 3604 4739 5420 5611 Size: 219 1075 613 52 819 1135 681 191 Cuts at: 5611 826 Size: 1471 Fragments arranged by size: 1471 1135 1075 819 681 613 219 191 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6256 SmaI CCC'GGG Cuts at: 1872 3583 1872 Size: 1711 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2855 4360 5261 5523 820 Size: 197 926 912 1505 901 262 1553 Fragments arranged by size: 1553 1505 926 912 901 262 197 SnaBI TAC'GTA Cuts at: 493 3553 4047 493 Size: 3060 494 2702 Fragments arranged by size: 3060 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6256 SphI G_CATG'C Cuts at: 1132 2576 3110 3614 1132 Size: 1444 534 504 3774 Fragments arranged by size: 3774 1444 534 504 SspI AAT'ATT Cuts at: 5 52 4180 5 Size: 47 4128 2081 Fragments arranged by size: 4128 2081 47 StuI AGG'CCT Cuts at: 1517 3364 1517 Size: 1847 4409 StyI C'CwwG_G Cuts at: 513 1065 2911 3292 3695 513 Size: 552 1846 381 403 3074 Fragments arranged by size: 3074 1846 552 403 381 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2060 3157 3192 3469 4283 4300 4453 Size: 1255 480 1097 35 277 814 17 153 Cuts at: 4453 4638 5083 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1097 814 480 445 277 185 153 35 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2453 4502 6214 Size: 274 80 33 51 1443 476 2049 1712 Cuts at: 6214 96 Size: 138 Fragments arranged by size: 2049 1712 1443 476 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3132 3164 3379 3411 6216 6248 3132 Size: 32 215 32 2805 32 3140 Fragments arranged by size: 3140 2805 215 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3532 1316 Size: 2216 4040 XbaI T'CTAG_A Cuts at: 3592 3592 Size: 6256 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2642 2642 Size: 6256 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6256 XmaI C'CCGG_G Cuts at: 1870 3581 1870 Size: 1711 4545 XmnI GAAnn'nnTTC Cuts at: 2076 4385 2076 Size: 2309 3947 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4064 276 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI