Restriction map for
pFN21ASDA1438
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BciVI BclI BfrI BglII
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII DrdI
Eam1105I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 2279 AAATTT [GA]^AATT[CT]
AocI 1930 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1376 AGCGCT AGC^GCT
ApoI 2279 AAATTT [GA]^AATT[CT]
Asp700I 1734 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 961 GGCGCC GGCGC^C
BbsI 2337 GAAGAC GAAGAC(2/6)
BcgI 375 CGAGCAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 2337 GAAGAC GAAGAC(2/6)
BsaI 139 GAGACC GGTCTC(1/5)
BsiEI 97 CGGCCG CG[GA][CT]^CG
BsmI 2022 GAATGC GAATGC(1/-1)
BspHI 30 TCATGA T^CATGA
BssSI 1489 CTCGTG CACGAG(-5/-1)
BstAPI 2121 GCACCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1930 CCTCAGG CC^T[ACGT]AGG
EagI 97 CGGCCG C^GGCCG
EclXI 97 CGGCCG C^GGCCG
Eco47III 1376 AGCGCT AGC^GCT
Eco52I 97 CGGCCG C^GGCCG
Eco81I 1930 CCTCAGG CC^T[ACGT]AGG
KasI 961 GGCGCC G^GCGCC
MaeII 987 ACGT A^CGT
MseI 623 TTAA T^TAA
NaeI 1029 GCCGGC GCC^GGC
NarI 961 GGCGCC GG^CGCC
NgoMIV 1029 GCCGGC G^CCGGC
NspI 1616 GCATGC [GA]CATG^[CT]
PflMI 2235 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 30 TCATGA T^CATGA
SacI 2386 GAGCTC GAGCT^C
SbfI 1464 CCTGCAGG CCTGCA^GG
SfiI 1299 GGCCACGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 961 GGCGCC GGC^GCC
SphI 1616 GCATGC GCATG^C
Sse8387I 1464 CCTGCAGG CCTGCA^GG
Tru9I 623 TTAA T^TAA
Van91I 2235 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1734 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AccII 1561 CGCG CG^CG
1613 CGCG
AcyI 961 GGCGCC G[GA]^CG[CT]C
2435 GACGCC
BbvCI 2712 CCTCAGC CCTCAGC(-5/-2)
1285 GCTGAGG
BsaHI 961 GGCGCC G[GA]^CG[CT]C
2435 GACGCC
BsgI 1198 CTGCAC GTGCAG(16/14)
2773 CTGCAC
BsrFI 1029 GCCGGC [GA]^CCGG[CT]
1899 GCCGGT
BstUI 1561 CGCG CG^CG
1613 CGCG
Cfr10I 1029 GCCGGC [GA]^CCGG[CT]
1899 GCCGGT
Hin1I 961 GGCGCC G[GA]^CG[CT]C
2435 GACGCC
MvnI 1561 CGCG CG^CG
1613 CGCG
SapI 335 GCTCTTC GCTCTTC(1/4)
801 GCTCTTC
Tsp509I 2280 AATT ^AATT
2594 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI