Restriction map for
pFN21ASDA1438
(Linear) (Six-base) MAPSORT of: KIAA1438.seq Check: 2145 from: 1 to: 2793
KIAA1438.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 202 304 706 1084 1096 2182 2793
Size: 196 6 102 402 378 12 1086 611
Fragments arranged by size:
1086 611 402 378 196 102 12 6
AfeI AGC'GCT
Cuts at: 0 1378 2793
Size: 1378 1415
AleI CACnn'nnGTG
Cuts at: 0 1720 2793
Size: 1720 1073
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2485 2793
Size: 2485 308
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 75 107 1726 1758 2793
Size: 75 32 1619 32 1035
Fragments arranged by size:
1619 1035 75 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1074 1578 1638 1770 2092 2541 2793
Size: 1074 504 60 132 322 449 252
Fragments arranged by size:
1074 504 449 322 252 132 60
ApaI G_GGCC'C
Cuts at: 0 974 1507 1777 1853 1939 2793
Size: 974 533 270 76 86 854
Fragments arranged by size:
974 854 533 270 86 76
ApoI r'AATT_y
Cuts at: 0 2279 2793
Size: 2279 514
AvaI C'yCGr_G
Cuts at: 0 373 428 957 1098 1414 1508 1845 1894
Size: 373 55 529 141 316 94 337 49
Cuts at: 1894 2793
Size: 899
Fragments arranged by size:
899 529 373 337 316 141 94 55
49
BanI G'GyrC_C
Cuts at: 0 753 961 1102 1714 2793
Size: 753 208 141 612 1079
Fragments arranged by size:
1079 753 612 208 141
BanII G_rGCy'C
Cuts at: 0 974 1026 1170 1345 1507 1534 1683 1777
Size: 974 52 144 175 162 27 149 94
Cuts at: 1777 1853 1895 1939 2056 2390 2507 2615 2793
Size: 76 42 44 117 334 117 108 178
Fragments arranged by size:
974 334 178 175 162 149 144 117
117 108 94 76 52 44 42 27
BbeI G_GCGC'C
Cuts at: 0 965 2793
Size: 965 1828
BbsI GAAGACnn'nnnn_
Cuts at: 0 2344 2793
Size: 2344 449
BbvCI CC'TCA_GC
Cuts at: 0 1286 2713 2793
Size: 1286 1427 80
Fragments arranged by size:
1427 1286 80
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 364 398 2793
Size: 364 34 2395
Fragments arranged by size:
2395 364 34
BglI GCCn_nnn'nGGC
Cuts at: 0 978 1030 1174 1264 1306 2793
Size: 978 52 144 90 42 1487
Fragments arranged by size:
1487 978 144 90 52 42
BlpI GC'TnA_GC
Cuts at: 0 149 1243 1387 1756 2384 2542 2698 2793
Size: 149 1094 144 369 628 158 156 95
Fragments arranged by size:
1094 628 369 158 156 149 144 95
Bme1580I G_kGCm'C
Cuts at: 0 756 974 1105 1507 1777 1853 1939 2793
Size: 756 218 131 402 270 76 86 854
Fragments arranged by size:
854 756 402 270 218 131 86 76
BmgBI CAC'GTC
Cuts at: 0 988 2793
Size: 988 1805
BmrI ACTGGGnnnn_n'
Cuts at: 0 913 1908 2787 2793
Size: 913 995 879 6
Fragments arranged by size:
995 913 879 6
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 442 793 936 1265 1684 1728 2069
Size: 145 297 351 143 329 419 44 341
Cuts at: 2069 2137 2396 2461 2473 2692 2793
Size: 68 259 65 12 219 101
Fragments arranged by size:
419 351 341 329 297 259 219 145
143 101 68 65 44 12
Bpu10I CC'TnA_GC
Cuts at: 0 1286 1528 1889 2609 2713 2793
Size: 1286 242 361 720 104 80
Fragments arranged by size:
1286 720 361 242 104 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 1636 2214 2793
Size: 691 945 578 579
Fragments arranged by size:
945 691 579 578
BsaI GGTCTCn'nnnn_
Cuts at: 0 133 2793
Size: 133 2660
BsaHI Gr'CG_yC
Cuts at: 0 962 2436 2793
Size: 962 1474 357
Fragments arranged by size:
1474 962 357
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1063 1093 1726 1756 2411 2441 2665 2695
Size: 1063 30 633 30 655 30 224 30
Cuts at: 2695 2793
Size: 98
Fragments arranged by size:
1063 655 633 224 98 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 530 1513 2399 2793
Size: 530 983 886 394
Fragments arranged by size:
983 886 530 394
BseYI C'CCAG_C
Cuts at: 0 568 1230 1682 1795 1809 1939 2057 2072
Size: 568 662 452 113 14 130 118 15
Cuts at: 2072 2614 2728 2793
Size: 542 114 65
Fragments arranged by size:
662 568 542 452 130 118 114 113
65 15 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1183 2758 2793
Size: 1183 1575 35
Fragments arranged by size:
1575 1183 35
BsiEI CG_ry'CG
Cuts at: 0 100 2793
Size: 100 2693
BsiHKAI G_wGCw'C
Cuts at: 0 1249 2123 2390 2793
Size: 1249 874 267 403
Fragments arranged by size:
1249 874 403 267
BsmI GAATG_Cn'
Cuts at: 0 2028 2793
Size: 2028 765
Bsp1286I G_dGCh'C
Cuts at: 0 756 974 1026 1105 1170 1249 1345 1507
Size: 756 218 52 79 65 79 96 162
Cuts at: 1507 1534 1683 1777 1853 1895 1939 2056 2123
Size: 27 149 94 76 42 44 117 67
Cuts at: 2123 2390 2507 2615 2793
Size: 267 117 108 178
Fragments arranged by size:
756 267 218 178 162 149 117 117
108 96 94 79 79 76 67 65
52 44 42 27
BspHI T'CATG_A
Cuts at: 0 30 2793
Size: 30 2763
BsrBI CCG'CTC
Cuts at: 0 54 1647 1780 2273 2793
Size: 54 1593 133 493 520
Fragments arranged by size:
1593 520 493 133 54
BsrFI r'CCGG_y
Cuts at: 0 1029 1899 2793
Size: 1029 870 894
Fragments arranged by size:
1029 894 870
BssSI C'ACGA_G
Cuts at: 0 1489 2793
Size: 1489 1304
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2127 2793
Size: 2127 666
BstXI CCAn_nnnn'nTGG
Cuts at: 0 246 300 2631 2793
Size: 246 54 2331 162
Fragments arranged by size:
2331 246 162 54
BstYI r'GATC_y
Cuts at: 0 782 818 1484 2793
Size: 782 36 666 1309
Fragments arranged by size:
1309 782 666 36
Bsu36I CC'TnA_GG
Cuts at: 0 1931 2793
Size: 1931 862
BtgI C'CryG_G
Cuts at: 0 239 761 1301 1859 2579 2624 2793
Size: 239 522 540 558 720 45 169
Fragments arranged by size:
720 558 540 522 239 169 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 374 1412 2793
Size: 374 1038 1381
Fragments arranged by size:
1381 1038 374
BtsI GCAGTG_nn'
Cuts at: 0 442 596 1087 1320 1381 1477 2125 2549
Size: 442 154 491 233 61 96 648 424
Cuts at: 2549 2793
Size: 244
Fragments arranged by size:
648 491 442 424 244 233 154 96
61
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 459 494 2793
Size: 459 35 2299
Fragments arranged by size:
2299 459 35
EaeI y'GGCC_r
Cuts at: 0 97 1031 1298 1307 2646 2757 2793
Size: 97 934 267 9 1339 111 36
Fragments arranged by size:
1339 934 267 111 97 36 9
EagI C'GGCC_G
Cuts at: 0 97 2793
Size: 97 2696
EarI CTCTTCn'nnn_
Cuts at: 0 131 178 342 808 2740 2793
Size: 131 47 164 466 1932 53
Fragments arranged by size:
1932 466 164 131 53 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1561 2793
Size: 1561 1232
EcoICRI GAG'CTC
Cuts at: 0 2388 2793
Size: 2388 405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 196 202 304 442 706 793 936
Size: 145 51 6 102 138 264 87 143
Cuts at: 936 1084 1096 1265 1684 1728 2069 2137 2182
Size: 148 12 169 419 44 341 68 45
Cuts at: 2182 2396 2461 2473 2692 2793
Size: 214 65 12 219 101
Fragments arranged by size:
419 341 264 219 214 169 148 145
143 138 102 101 87 68 65 51
45 44 12 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1160 1201 1492 1948 2411 2793
Size: 1160 41 291 456 463 382
Fragments arranged by size:
1160 463 456 382 291 41
EcoO109I rG'GnC_Cy
Cuts at: 0 894 911 971 1176 1503 1504 1517 1774
Size: 894 17 60 205 327 1 13 257
Cuts at: 1774 1849 1850 1927 1935 1936 1991 2144 2351
Size: 75 1 77 8 1 55 153 207
Cuts at: 2351 2793
Size: 442
Fragments arranged by size:
894 442 327 257 207 205 153 77
75 60 55 17 13 8 1 1
1
HaeII r_GCGC'y
Cuts at: 0 965 1128 1380 1609 2092 2793
Size: 965 163 252 229 483 701
Fragments arranged by size:
965 701 483 252 229 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 352 384 1390 1422 1431 1463 2290 2314
Size: 352 32 1006 32 9 32 827 24
Cuts at: 2314 2322 2346 2665 2697 2793
Size: 8 24 319 32 96
Fragments arranged by size:
1006 827 352 319 96 32 32 32
32 24 24 9 8
KasI G'GCGC_C
Cuts at: 0 961 2793
Size: 961 1832
MscI TGG'CCA
Cuts at: 0 1300 1309 2648 2759 2793
Size: 1300 9 1339 111 34
Fragments arranged by size:
1339 1300 111 34 9
MslI CAynn'nnrTG
Cuts at: 0 298 1051 1720 2793
Size: 298 753 669 1073
Fragments arranged by size:
1073 753 669 298
MspA1I CmG'CkG
Cuts at: 0 174 813 908 1234 1285 1458 1638 1662
Size: 174 639 95 326 51 173 180 24
Cuts at: 1662 1748 1755 2772 2793
Size: 86 7 1017 21
Fragments arranged by size:
1017 639 326 180 174 173 95 86
51 24 21 7
NaeI GCC'GGC
Cuts at: 0 1031 2793
Size: 1031 1762
NarI GG'CG_CC
Cuts at: 0 962 2793
Size: 962 1831
NcoI C'CATG_G
Cuts at: 0 239 761 2579 2624 2793
Size: 239 522 1818 45 169
Fragments arranged by size:
1818 522 239 169 45
NgoMIV G'CCGG_C
Cuts at: 0 1029 2793
Size: 1029 1764
NspI r_CATG'y
Cuts at: 0 1620 2793
Size: 1620 1173
PasI CC'CwG_GG
Cuts at: 0 399 897 967 1534 1770 2793
Size: 399 498 70 567 236 1023
Fragments arranged by size:
1023 567 498 399 236 70
PflMI CCAn_nnn'nTGG
Cuts at: 0 2241 2793
Size: 2241 552
PfoI T'CCnGG_A
Cuts at: 0 2450 2558 2793
Size: 2450 108 235
Fragments arranged by size:
2450 235 108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 431 463 985 1017 1726 1758 2793
Size: 431 32 522 32 709 32 1035
Fragments arranged by size:
1035 709 522 431 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 911 1517 1991 2144 2351 2793
Size: 911 606 474 153 207 442
Fragments arranged by size:
911 606 474 442 207 153
PspOMI G'GGCC_C
Cuts at: 0 970 1503 1773 1849 1935 2793
Size: 970 533 270 76 86 858
Fragments arranged by size:
970 858 533 270 86 76
PstI C_TGCA'G
Cuts at: 0 791 818 1469 1484 1583 1661 2132 2793
Size: 791 27 651 15 99 78 471 661
Fragments arranged by size:
791 661 651 471 99 78 27 15
PvuII CAG'CTG
Cuts at: 0 174 813 1285 1458 1638 1662 1748 1755
Size: 174 639 472 173 180 24 86 7
Cuts at: 1755 2772 2793
Size: 1017 21
Fragments arranged by size:
1017 639 472 180 174 173 86 24
21 7
SacI G_AGCT'C
Cuts at: 0 2390 2793
Size: 2390 403
SanDI GG'GwC_CC
Cuts at: 0 911 1991 2144 2351 2793
Size: 911 1080 153 207 442
Fragments arranged by size:
1080 911 442 207 153
SapI GCTCTTCn'nnn_
Cuts at: 0 342 808 2793
Size: 342 466 1985
Fragments arranged by size:
1985 466 342
SbfI CC_TGCA'GG
Cuts at: 0 1469 2793
Size: 1469 1324
SfcI C'TryA_G
Cuts at: 0 787 814 1465 1480 1579 1657 2128 2793
Size: 787 27 651 15 99 78 471 665
Fragments arranged by size:
787 665 651 471 99 78 27 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1306 2793
Size: 1306 1487
SfoI GGC'GCC
Cuts at: 0 963 2793
Size: 963 1830
SmaI CCC'GGG
Cuts at: 0 1416 1510 1847 2793
Size: 1416 94 337 946
Fragments arranged by size:
1416 946 337 94
SmlI C'TyrA_G
Cuts at: 0 706 1651 2229 2793
Size: 706 945 578 564
Fragments arranged by size:
945 706 578 564
SphI G_CATG'C
Cuts at: 0 1620 2793
Size: 1620 1173
StuI AGG'CCT
Cuts at: 0 231 1274 1471 2793
Size: 231 1043 197 1322
Fragments arranged by size:
1322 1043 231 197
StyI C'CwwG_G
Cuts at: 0 239 677 761 1172 2579 2624 2793
Size: 239 438 84 411 1407 45 169
Fragments arranged by size:
1407 438 411 239 169 84 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 487 1799 2138 2592 2793
Size: 487 1312 339 454 201
Fragments arranged by size:
1312 487 454 339 201
TatI w'GTAC_w
Cuts at: 0 719 2793
Size: 719 2074
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1469 1501 2411 2443 2767 2793
Size: 1469 32 910 32 324 26
Fragments arranged by size:
1469 910 324 32 32 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 246 1317 2622 2793
Size: 246 1071 1305 171
Fragments arranged by size:
1305 1071 246 171
XmaI C'CCGG_G
Cuts at: 0 1414 1508 1845 2793
Size: 1414 94 337 948
Fragments arranged by size:
1414 948 337 94
XmnI GAAnn'nnTTC
Cuts at: 0 1738 2793
Size: 1738 1055
Enzymes that do cut:
AcuI AfeI AleI AlfI AloI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BsrBI BsrFI BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII
SacI SanDI SapI SbfI SfcI SfiI SfoI SmaI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BclI BfrBI BglII BmtI BplI BsaAI BsaBI
BsaWI BsiWI BsmBI BspEI BspMI BsrDI BsrGI BssHII
BstBI BstEII BstZ17I ClaI DraI DraIII DrdI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI ScaI SexAI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XhoI ZraI