Restriction map for
pFN21ASDA1433
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BclI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MflI
MluI MroI MvnI NaeI NarI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AflII 247 CTTAAG C^TTAAG
Aor51HI 512 AGCGCT AGC^GCT
BalI 1047 TGGCCA TGG^CCA
BanI 320 GGCACC G^G[CT][GA]CC
BbsI 1837 GAAGAC GAAGAC(2/6)
BciVI 372 GGATAC GTATCC(6/5)
BcnI 636 CCGGG CC^[GC]GG
BfrI 247 CTTAAG C^TTAAG
BglI 91 GCCTTAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 606 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 606 GCTGAGC GC^T[ACGT]AGC
BpuAI 1837 GAAGAC GAAGAC(2/6)
BsaWI 773 ACCGGA [AT]^CCGG[AT]
BsgI 225 CTGCAC GTGCAG(16/14)
BsrFI 1290 GCCGGT [GA]^CCGG[CT]
BstXI 1490 CCAAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 606 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1290 GCCGGT [GA]^CCGG[CT]
DraI 807 TTTAAA TTT^AAA
EaeI 1047 TGGCCA [CT]^GGCC[GA]
Eco47III 512 AGCGCT AGC^GCT
EcoNI 1648 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 966 CCATGG C^C[AT][AT]GG
FauI 518 CCCGC CCCGC(4/6)
HaeII 512 AGCGCT [GA]GCGC^[CT]
MfeI 865 CAATTG C^AATTG
MluNI 1047 TGGCCA TGG^CCA
MscI 1047 TGGCCA TGG^CCA
MunI 865 CAATTG C^AATTG
NciI 636 CCGGG CC^[GC]GG
NcoI 966 CCATGG C^CATGG
NdeI 1031 CATATG CA^TATG
NheI 1324 GCTAGC G^CTAGC
PshAI 1191 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1230 GAGCTC GAGCT^C
StyI 966 CCATGG C^C[AT][AT]GG
XcmI 1419 CCAACAAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 442 GAATTT [GA]^AATT[CT]
1393 AAATTT
AfaI 1294 GTAC GT^AC
1350 GTAC
AlwNI 899 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
1848 CAGCAGCTG
ApoI 442 GAATTT [GA]^AATT[CT]
1393 AAATTT
AspHI 1230 GAGCTC G[AT]GC[AT]^C
1522 GTGCTC
BsiHKAI 1230 GAGCTC G[AT]GC[AT]^C
1522 GTGCTC
BsiYI 612 CCTGAAATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1648 CCTACTCCAGG
BslI 612 CCTGAAATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1648 CCTACTCCAGG
CfoI 285 GCGC GCG^C
513 GCGC
DraII 74 GGGACCT [GA]G^G[ACGT]CC[CT]
824 AGGACCT
DsaI 966 CCATGG C^C[GA][CT]GG
1120 CCACGG
EcoO109I 74 GGGACCT [GA]G^G[ACGT]CC[CT]
824 AGGACCT
HhaI 285 GCGC GCG^C
513 GCGC
HinP1I 285 GCGC G^CGC
513 GCGC
PleI 429 GACTC GAGTC(4/5)
1191 GACTC
PpuMI 74 GGGACCT [GA]G^G[AT]CC[CT]
824 AGGACCT
PstI 1088 CTGCAG CTGCA^G
1832 CTGCAG
RsaI 1294 GTAC GT^AC
1350 GTAC
SphI 287 GCATGC GCATG^C
363 GCATGC
TaqI 731 TCGA T^CGA
1599 TCGA
TthHB8I 731 TCGA T^CGA
1599 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PvuII SapI
Sau3AI Sau96I ScrFI SfcI SmlI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI