Restriction map for
pFN21ASDA1433
(Linear) (Six-base) MAPSORT of: KIAA1433.seq Check: 2620 from: 1 to: 1917
KIAA1433.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 401 1917
Size: 211 190 1516
Fragments arranged by size:
1516 211 190
AfeI AGC'GCT
Cuts at: 0 514 1917
Size: 514 1403
AflII C'TTAA_G
Cuts at: 0 247 1917
Size: 247 1670
AleI CACnn'nnGTG
Cuts at: 0 1519 1917
Size: 1519 398
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 292 380 1917
Size: 292 88 1537
Fragments arranged by size:
1537 292 88
AlwNI CAG_nnn'CTG
Cuts at: 0 904 1853 1917
Size: 904 949 64
Fragments arranged by size:
949 904 64
ApoI r'AATT_y
Cuts at: 0 442 1393 1917
Size: 442 951 524
Fragments arranged by size:
951 524 442
BanI G'GyrC_C
Cuts at: 0 320 1917
Size: 320 1597
BanII G_rGCy'C
Cuts at: 0 1234 1444 1681 1917
Size: 1234 210 237 236
Fragments arranged by size:
1234 237 236 210
BbsI GAAGACnn'nnnn_
Cuts at: 0 1844 1917
Size: 1844 73
BciVI GTATCCnnnnn_n'
Cuts at: 0 366 1917
Size: 366 1551
BglI GCCn_nnn'nGGC
Cuts at: 0 97 1917
Size: 97 1820
BlpI GC'TnA_GC
Cuts at: 0 607 1917
Size: 607 1310
BmrI ACTGGGnnnn_n'
Cuts at: 0 1007 1202 1917
Size: 1007 195 715
Fragments arranged by size:
1007 715 195
BmtI G_CTAG'C
Cuts at: 0 1328 1917
Size: 1328 589
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 446 531 1917
Size: 446 85 1386
Fragments arranged by size:
1386 446 85
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1178 1512 1637 1673 1917
Size: 1178 334 125 36 244
Fragments arranged by size:
1178 334 244 125 36
Bpu10I CC'TnA_GC
Cuts at: 0 1675 1917
Size: 1675 242
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 571 1917
Size: 364 207 1346
Fragments arranged by size:
1346 364 207
BsaWI w'CCGG_w
Cuts at: 0 773 1917
Size: 773 1144
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 377 407 1260 1290 1758 1788 1917
Size: 377 30 853 30 468 30 129
Fragments arranged by size:
853 468 377 129 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 523 1248 1251 1917
Size: 523 725 3 666
Fragments arranged by size:
725 666 523 3
BseYI C'CCAG_C
Cuts at: 0 296 1243 1917
Size: 296 947 674
Fragments arranged by size:
947 674 296
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 1917
Size: 210 1707
BsiHKAI G_wGCw'C
Cuts at: 0 1234 1526 1917
Size: 1234 292 391
Fragments arranged by size:
1234 391 292
Bsp1286I G_dGCh'C
Cuts at: 0 1234 1444 1526 1681 1917
Size: 1234 210 82 155 236
Fragments arranged by size:
1234 236 210 155 82
BsrFI r'CCGG_y
Cuts at: 0 1290 1917
Size: 1290 627
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1497 1917
Size: 1497 420
BtgI C'CryG_G
Cuts at: 0 966 1120 1917
Size: 966 154 797
Fragments arranged by size:
966 797 154
BtsI GCAGTG_nn'
Cuts at: 0 271 1250 1613 1829 1917
Size: 271 979 363 216 88
Fragments arranged by size:
979 363 271 216 88
DraI TTT'AAA
Cuts at: 0 809 1917
Size: 809 1108
EaeI y'GGCC_r
Cuts at: 0 1047 1917
Size: 1047 870
EarI CTCTTCn'nnn_
Cuts at: 0 500 625 650 761 1224 1290 1863 1917
Size: 500 125 25 111 463 66 573 54
Fragments arranged by size:
573 500 463 125 111 66 54 25
EcoICRI GAG'CTC
Cuts at: 0 1232 1917
Size: 1232 685
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 401 1178 1512 1637 1673 1917
Size: 211 190 777 334 125 36 244
Fragments arranged by size:
777 334 244 211 190 125 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1652 1917
Size: 1652 265
EcoO109I rG'GnC_Cy
Cuts at: 0 75 825 1917
Size: 75 750 1092
Fragments arranged by size:
1092 750 75
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 79 699 1917
Size: 79 620 1218
Fragments arranged by size:
1218 620 79
HaeII r_GCGC'y
Cuts at: 0 516 1917
Size: 516 1401
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 377 409 1176 1208 1788 1820 1917
Size: 377 32 767 32 580 32 97
Fragments arranged by size:
767 580 377 97 32 32 32
MfeI C'AATT_G
Cuts at: 0 865 1917
Size: 865 1052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 1917
Size: 419 1498
MscI TGG'CCA
Cuts at: 0 1049 1917
Size: 1049 868
MslI CAynn'nnrTG
Cuts at: 0 400 544 919 1519 1917
Size: 400 144 375 600 398
Fragments arranged by size:
600 400 398 375 144
MspA1I CmG'CkG
Cuts at: 0 300 360 501 667 1497 1853 1917
Size: 300 60 141 166 830 356 64
Fragments arranged by size:
830 356 300 166 141 64 60
NcoI C'CATG_G
Cuts at: 0 966 1917
Size: 966 951
NdeI CA'TA_TG
Cuts at: 0 1032 1917
Size: 1032 885
NheI G'CTAG_C
Cuts at: 0 1324 1917
Size: 1324 593
NspI r_CATG'y
Cuts at: 0 279 291 367 405 1917
Size: 279 12 76 38 1512
Fragments arranged by size:
1512 279 76 38 12
PfoI T'CCnGG_A
Cuts at: 0 1689 1917
Size: 1689 228
PpuMI rG'GwC_Cy
Cuts at: 0 75 825 1917
Size: 75 750 1092
Fragments arranged by size:
1092 750 75
PshAI GACnn'nnGTC
Cuts at: 0 1195 1917
Size: 1195 722
PstI C_TGCA'G
Cuts at: 0 1092 1836 1917
Size: 1092 744 81
Fragments arranged by size:
1092 744 81
PvuII CAG'CTG
Cuts at: 0 300 501 667 1497 1853 1917
Size: 300 201 166 830 356 64
Fragments arranged by size:
830 356 300 201 166 64
SacI G_AGCT'C
Cuts at: 0 1234 1917
Size: 1234 683
SapI GCTCTTCn'nnn_
Cuts at: 0 625 1290 1863 1917
Size: 625 665 573 54
Fragments arranged by size:
665 625 573 54
SfcI C'TryA_G
Cuts at: 0 169 1088 1435 1832 1917
Size: 169 919 347 397 85
Fragments arranged by size:
919 397 347 169 85
SmlI C'TyrA_G
Cuts at: 0 247 343 550 1917
Size: 247 96 207 1367
Fragments arranged by size:
1367 247 207 96
SphI G_CATG'C
Cuts at: 0 291 367 1917
Size: 291 76 1550
Fragments arranged by size:
1550 291 76
StyI C'CwwG_G
Cuts at: 0 966 1917
Size: 966 951
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1160 1755 1764 1917
Size: 1160 595 9 153
Fragments arranged by size:
1160 595 153 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1605 1637 1758 1790 1917
Size: 1605 32 121 32 127
Fragments arranged by size:
1605 127 121 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1426 1917
Size: 1426 491
Enzymes that do cut:
AcuI AfeI AflII AleI AlfI AlwNI ApoI BanI
BanII BbsI BciVI BglI BlpI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BsrFI BstXI BtgI BtsI DraI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
MfeI MmeI MscI MslI MspA1I NcoI NdeI NheI
NspI PfoI PpuMI PshAI PstI PvuII SacI SapI
SfcI SmlI SphI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BclI BfrBI BglII
Bme1580I BmgBI BsaI BsaAI BsaBI BsaHI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MluI NaeI NarI NgoMIV NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI TatI Tth111I
XbaI XhoI XmaI XmnI ZraI