Restriction map for
pFN21AA1429
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# NO CUT ENZYMES ( 89 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I ApaI
ApaLI AscI Asp718I AspEI AviII
AvrII BanI BbeI BbrPI BbvCI
BcnI BlnI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspEI
BsrBI BsrFI BssHII BstBI BstUI
Cfr10I CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco52I FauI
FseI FspI HapII HgaI Hin1I
HpaII KasI KpnI KspI MluI
MroI MspI MvnI NaeI NarI
NciI NcoI NgoMIV NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AocI 823 CCTGAGG CC^T[ACGT]AGG
AspI 1522 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3070 GGATCC G^GATCC
BcgI 1764 CGACACAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 951 GTATCC GTATCC(6/5)
BglI 2955 GCCTTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 58 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 58 GCTGAGC GC^T[ACGT]AGC
Bsp1407I 2341 TGTACA T^GTACA
BspDI 2755 ATCGAT AT^CGAT
BspLU11I 4036 ACATGT A^CATGT
BsrGI 2341 TGTACA T^GTACA
BssSI 5367 CACGAG CACGAG(-5/-1)
BstAPI 3500 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2241 CCAATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 823 CCTGAGG CC^T[ACGT]AGG
CelII 58 GCTGAGC GC^T[ACGT]AGC
ClaI 2755 ATCGAT AT^CGAT
Eco81I 823 CCTGAGG CC^T[ACGT]AGG
EcoNI 2836 CCTGTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 675 GAATTC G^AATTC
MspA1I 2429 CAGCTG C[AC]G^C[GT]G
NheI 3999 GCTAGC G^CTAGC
PaeR7I 5086 CTCGAG C^TCGAG
PflFI 1522 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 604 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 558 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 4035 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 4304 CTGCAG CTGCA^G
PvuII 2429 CAGCTG CAG^CTG
SexAI 3667 ACCAGGT A^CC[AT]GGT
SspBI 2341 TGTACA T^GTACA
SspI 698 AATATT AAT^ATT
Tth111I 1522 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 604 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5202 TCTAGA T^CTAGA
XcmI 2498 CCAAAGAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 5086 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AflII 1495 CTTAAG C^TTAAG
4438 CTTAAG
AseI 3736 ATTAAT AT^TAAT
4297 ATTAAT
AsnI 3736 ATTAAT AT^TAAT
4297 ATTAAT
BfrI 1495 CTTAAG C^TTAAG
4438 CTTAAG
BsaAI 1480 CACGTA [CT]AC^GT[GA]
2801 TACGTG
BspHI 399 TCATGA T^CATGA
4905 TCATGA
BspMI 3505 ACCTGC ACCTGC(4/8)
4830 ACCTGC
Bst1107I 3651 GTATAC GTA^TAC
4895 GTATAC
BstZ17I 3651 GTATAC GTA^TAC
4895 GTATAC
DraII 558 AGGACCC [GA]G^G[ACGT]CC[CT]
5135 AGGCCCT
EcoO109I 558 AGGACCC [GA]G^G[ACGT]CC[CT]
5135 AGGCCCT
EcoRV 2021 GATATC GAT^ATC
3187 GATATC
HpaI 1152 GTTAAC GTT^AAC
3048 GTTAAC
PshBI 3736 ATTAAT AT^TAAT
4297 ATTAAT
RcaI 399 TCATGA T^CATGA
4905 TCATGA
SapI 3679 GCTCTTC GCTCTTC(1/4)
3902 GCTCTTC
ScaI 2489 AGTACT AGT^ACT
4500 AGTACT
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# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
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AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI Aor51HI ApoI
Asp700I AspHI AvaI AvaII BalI
BanII BbsI BbvI BclI BfaI
BglII BmyI BpmI BpuAI BsaBI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EaeI EarI Eco47III EcoO65I EcoRII
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeII HaeIII HhaI HinP1I HincII
HindII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MunI
MvaI MwoI NdeI NdeII NlaIII
NlaIV NsiI NspI PleI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI