(Circular) (Six-base) MAPSORT of: pFN21AA1429.seq Check: 2641 from: 1 to: 10096 pFN21AA1429 10096 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7906 278 Size: 53 83 186 936 6370 2468 Fragments arranged by size: 6370 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2817 3300 5657 6901 6946 7439 1937 Size: 880 483 2357 1244 45 493 4594 Fragments arranged by size: 4594 2357 1244 880 493 483 45 Acc65I G'GTAC_C Cuts at: 7417 7417 Size: 10096 AclI AA'CG_TT Cuts at: 1563 8223 8596 1563 Size: 6660 373 3063 Fragments arranged by size: 6660 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2982 3006 3040 4923 5091 5343 5619 5934 Size: 1350 24 34 1883 168 252 276 315 Cuts at: 5934 6512 6639 8159 9543 9811 1632 Size: 578 127 1520 1384 268 1917 Fragments arranged by size: 1917 1883 1520 1384 1350 578 315 276 268 252 168 127 34 24 AfeI AGC'GCT Cuts at: 2062 3336 5112 6716 2062 Size: 1274 1776 1604 5442 Fragments arranged by size: 5442 1776 1604 1274 AflII C'TTAA_G Cuts at: 820 1017 3500 6443 820 Size: 197 2483 2943 4473 Fragments arranged by size: 4473 2943 2483 197 AflIII A'CryG_T Cuts at: 1203 4807 4914 6041 7393 8995 9972 1203 Size: 3604 107 1127 1352 1602 977 1327 Fragments arranged by size: 3604 1602 1352 1327 1127 977 107 AgeI A'CCGG_T Cuts at: 8927 8927 Size: 10096 AhdI GACnn_n'nnGTC Cuts at: 8825 8825 Size: 10096 AleI CACnn'nnGTG Cuts at: 1166 4098 1166 Size: 2932 7164 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 10096 AlwNI CAG_nnn'CTG Cuts at: 2615 3779 6454 6535 7839 9411 2615 Size: 1164 2675 81 1304 1572 3300 Fragments arranged by size: 3300 2675 1572 1304 1164 81 ApaLI G'TGCA_C Cuts at: 8153 9309 8153 Size: 1156 8940 ApoI r'AATT_y Cuts at: 1493 2680 2904 3127 3222 3322 4189 5181 6200 Size: 1187 224 223 95 100 867 992 1019 Cuts at: 6200 6213 6355 7405 7631 7783 9892 1493 Size: 13 142 1050 226 152 2109 1697 Fragments arranged by size: 2109 1697 1187 1050 1019 992 867 226 224 223 152 142 100 95 13 AseI AT'TA_AT Cuts at: 160 1032 5742 6303 8650 160 Size: 872 4710 561 2347 1606 Fragments arranged by size: 4710 2347 1606 872 561 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10096 AvaI C'yCGr_G Cuts at: 1870 1943 2056 7091 7193 7421 1870 Size: 73 113 5035 102 228 4545 Fragments arranged by size: 5035 4545 228 113 102 73 BamHI G'GATC_C Cuts at: 5075 7426 7793 5075 Size: 2351 367 7378 Fragments arranged by size: 7378 2351 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 7417 8872 618 Size: 325 217 627 5630 1455 1842 Fragments arranged by size: 5630 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2152 2664 3418 4207 6280 6850 7415 1391 Size: 761 512 754 789 2073 570 565 4072 Fragments arranged by size: 4072 2073 789 761 754 570 565 512 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2994 3737 4148 4578 5625 928 Size: 971 1095 743 411 430 1047 5399 Fragments arranged by size: 5399 1095 1047 971 743 430 411 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3758 3792 8287 8321 1903 Size: 34 1821 34 4495 34 3678 Fragments arranged by size: 4495 3678 1821 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2967 7988 9198 2967 Size: 5021 1210 3865 Fragments arranged by size: 5021 3865 1210 BclI T'GATC_A Cuts at: 1548 3450 3481 3763 4222 5878 6580 1548 Size: 1902 31 282 459 1656 702 5064 Fragments arranged by size: 5064 1902 1656 702 459 282 31 BfrBI ATG'CAT Cuts at: 4271 4560 6961 4271 Size: 289 2401 7406 Fragments arranged by size: 7406 2401 289 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4966 8707 136 Size: 107 122 71 631 605 3294 3741 1525 Fragments arranged by size: 3741 3294 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4375 4861 6634 10091 4375 Size: 486 1773 3457 4380 Fragments arranged by size: 4380 3457 1773 486 BlpI GC'TnA_GC Cuts at: 2064 7513 2064 Size: 5449 4647 Bme1580I G_kGCm'C Cuts at: 1790 2248 8157 9313 1790 Size: 458 5909 1156 2573 Fragments arranged by size: 5909 2573 1156 458 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10096 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 7827 8785 442 Size: 416 53 83 399 24 6410 958 1753 Fragments arranged by size: 6410 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 6008 1056 Size: 4952 5144 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5959 5959 Size: 10096 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2544 3975 4478 5835 5954 6476 6501 6726 Size: 1305 1431 503 1357 119 522 25 225 Cuts at: 6726 7050 8756 1239 Size: 324 1706 2579 Fragments arranged by size: 2579 1706 1431 1357 1305 522 503 324 225 119 25 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3402 3806 6990 7251 8221 9086 9384 3402 Size: 404 3184 261 970 865 298 4114 Fragments arranged by size: 4114 3184 970 865 404 298 261 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4247 5814 6263 6788 8759 882 Size: 617 2748 1567 449 525 1971 2219 Fragments arranged by size: 2748 2219 1971 1567 617 525 449 BsaAI yAC'GTr Cuts at: 493 1204 3487 4808 7887 493 Size: 711 2283 1321 3079 2702 Fragments arranged by size: 3079 2702 2283 1321 711 BsaBI GATnn'nnATC Cuts at: 3837 6408 6633 7332 10090 3837 Size: 2571 225 699 2758 3843 Fragments arranged by size: 3843 2758 2571 699 225 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7903 8285 8920 275 Size: 53 83 186 936 6370 382 635 1451 Fragments arranged by size: 6370 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 8529 8927 9201 9348 9759 8529 Size: 398 274 147 411 8866 Fragments arranged by size: 8866 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3973 4003 5115 5145 5331 Size: 30 165 30 2570 30 1112 30 186 Cuts at: 5331 5361 5808 5838 1178 Size: 30 447 30 5436 Fragments arranged by size: 5436 2570 1112 447 186 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2547 2628 2846 4247 7079 1186 Size: 1361 81 218 1401 2832 4203 Fragments arranged by size: 4203 2832 1401 1361 218 81 BseYI C'CCAG_C Cuts at: 2451 3377 9299 9795 2451 Size: 926 5922 496 2752 Fragments arranged by size: 5922 2752 926 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4376 5011 5562 1163 Size: 573 2640 635 551 5697 Fragments arranged by size: 5697 2640 635 573 551 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7546 8307 9335 1540 Size: 275 186 5545 761 1028 2301 Fragments arranged by size: 5545 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4093 4207 5141 6127 6280 6850 7080 8157 8242 Size: 114 934 986 153 570 230 1077 85 Cuts at: 8242 9313 4093 Size: 1071 4876 Fragments arranged by size: 4876 1077 1071 986 934 570 230 153 114 85 BsmI GAATG_Cn' Cuts at: 7607 7700 7607 Size: 93 10003 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2152 2248 2664 3418 4093 4207 5141 Size: 399 362 96 416 754 675 114 934 Cuts at: 5141 6127 6280 6850 7080 7415 8157 8242 9313 Size: 986 153 570 230 335 742 85 1071 Cuts at: 9313 1391 Size: 2174 Fragments arranged by size: 2174 1071 986 934 754 742 675 570 416 399 362 335 230 153 114 96 85 BspEI T'CCGG_A Cuts at: 9759 9759 Size: 10096 BspHI T'CATG_A Cuts at: 2404 6910 7985 9715 2404 Size: 4506 1075 1730 2785 Fragments arranged by size: 4506 2785 1730 1075 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5519 6844 7451 844 Size: 4675 1325 607 3489 Fragments arranged by size: 4675 3489 1325 607 BsrBI CCG'CTC Cuts at: 1394 1823 7983 1394 Size: 429 6160 3507 Fragments arranged by size: 6160 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4304 4316 5372 5886 8591 8765 65 Size: 1183 3056 12 1056 514 2705 174 1396 Fragments arranged by size: 3056 2705 1396 1183 1056 514 174 12 BsrFI r'CCGG_y Cuts at: 1700 1810 8740 8927 1700 Size: 110 6930 187 2869 Fragments arranged by size: 6930 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4346 96 Size: 4250 5846 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10096 BssSI C'ACGA_G Cuts at: 7372 8156 9168 7372 Size: 784 1012 8300 Fragments arranged by size: 8300 1012 784 BstAPI GCAn_nnn'nTGC Cuts at: 794 5511 794 Size: 4717 5379 BstBI TT'CG_AA Cuts at: 8900 8900 Size: 10096 BstEII G'GTnAC_C Cuts at: 1184 3713 4057 6467 7177 1184 Size: 2529 344 2410 710 4103 Fragments arranged by size: 4103 2529 2410 710 344 BstXI CCAn_nnnn'nTGG Cuts at: 2011 4253 2011 Size: 2242 7854 BstYI r'GATC_y Cuts at: 1972 3838 4375 4861 5075 5788 6634 7426 7793 Size: 1866 537 486 214 713 846 792 367 Cuts at: 7793 8178 8195 9647 9733 9745 10091 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1866 1452 846 792 713 537 486 385 367 346 214 86 17 12 BstZ17I GTA'TAC Cuts at: 5658 6902 5658 Size: 1244 8852 Bsu36I CC'TnA_GG Cuts at: 2829 2829 Size: 10096 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 8605 Fragments arranged by size: 8605 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2036 487 Size: 683 56 810 8547 Fragments arranged by size: 8547 810 683 56 BtsI GCAGTG_nn' Cuts at: 2979 4797 7263 7299 7612 8406 8426 2979 Size: 1818 2466 36 313 794 20 4649 Fragments arranged by size: 4649 2466 1818 794 313 36 20 ClaI AT'CG_AT Cuts at: 1554 4761 1554 Size: 3207 6889 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10061 DraI TTT'AAA Cuts at: 2050 3321 3517 6096 7400 2050 Size: 1271 196 2579 1304 4746 Fragments arranged by size: 4746 2579 1304 1271 196 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9103 809 Size: 507 7787 1802 Fragments arranged by size: 7787 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3289 4441 5262 Size: 54 1425 50 275 18 1459 1152 821 Cuts at: 5262 7031 7543 8432 10061 8 Size: 1769 512 889 1629 43 Fragments arranged by size: 1769 1629 1459 1425 1152 889 821 512 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7543 1537 Size: 275 5731 4090 Fragments arranged by size: 5731 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2622 2721 2778 2832 2844 2859 3747 5043 Size: 1269 99 57 54 12 15 888 1296 Cuts at: 5043 5247 5373 5691 5914 6531 6615 7151 8026 Size: 204 126 318 223 617 84 536 875 Cuts at: 8026 1353 Size: 3423 Fragments arranged by size: 3423 1296 1269 888 875 617 536 318 223 204 126 99 84 57 54 15 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8683 9057 9203 387 Size: 1064 138 7094 374 146 1280 Fragments arranged by size: 7094 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4205 6278 6848 4205 Size: 2073 570 7453 Fragments arranged by size: 7453 2073 570 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2544 2982 3006 3040 3975 4478 4923 Size: 393 912 438 24 34 935 503 445 Cuts at: 4923 5091 5343 5619 5835 5934 5954 6476 6501 Size: 168 252 276 216 99 20 522 25 Cuts at: 6501 6512 6639 6726 7050 8159 8756 9543 9811 Size: 11 127 87 324 1109 597 787 268 Cuts at: 9811 1239 Size: 1524 Fragments arranged by size: 1524 1109 935 912 787 597 522 503 445 438 393 324 276 268 252 216 168 127 99 87 34 25 24 20 11 EcoNI CCTnn'n_nnAGG Cuts at: 4845 4845 Size: 10096 EcoO109I rG'GnC_Cy Cuts at: 2564 7141 2564 Size: 4577 5519 EcoRI G'AATT_C Cuts at: 2680 7405 7783 2680 Size: 4725 378 4993 Fragments arranged by size: 4993 4725 378 EcoRV GAT'ATC Cuts at: 4028 5194 4028 Size: 1166 8930 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3302 3353 5315 6673 3302 Size: 51 1962 1358 6725 Fragments arranged by size: 6725 1962 1358 51 FspI TGC'GCA Cuts at: 8602 8602 Size: 10096 HaeII r_GCGC'y Cuts at: 1678 2064 3338 5114 6718 9243 1678 Size: 386 1274 1776 1604 2525 2531 Fragments arranged by size: 2531 2525 1776 1604 1274 386 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2399 2431 3004 3036 3473 Size: 32 135 32 827 32 573 32 437 Cuts at: 3473 3505 3724 3756 3988 4020 4189 4221 4511 Size: 32 219 32 232 32 169 32 290 Cuts at: 4511 4543 5331 5363 5552 5584 6170 6202 6686 Size: 32 788 32 189 32 586 32 484 Cuts at: 6686 6718 8737 8769 8811 8843 1373 Size: 32 2019 32 42 32 2626 Fragments arranged by size: 2626 2019 827 788 586 573 484 437 290 232 219 189 169 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3159 4853 4978 5055 6655 7440 Size: 988 281 1221 1694 125 77 1600 785 Cuts at: 7440 7686 669 Size: 246 3079 Fragments arranged by size: 3079 1694 1600 1221 988 785 281 246 125 77 HindIII A'AGCT_T Cuts at: 748 3315 3519 3558 3602 3822 4634 5712 6377 Size: 2567 204 39 44 220 812 1078 665 Cuts at: 6377 748 Size: 4467 Fragments arranged by size: 4467 2567 1078 812 665 220 204 44 39 HpaI GTT'AAC Cuts at: 3159 5055 7686 3159 Size: 1896 2631 5569 Fragments arranged by size: 5569 2631 1896 KpnI G_GTAC'C Cuts at: 7421 7421 Size: 10096 MfeI C'AATT_G Cuts at: 3260 5552 6231 7695 3260 Size: 2292 679 1464 5661 Fragments arranged by size: 5661 2292 1464 679 MluI A'CGCG_T Cuts at: 9972 9972 Size: 10096 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3967 4152 5583 6405 6418 7051 9210 9394 Size: 2662 185 1431 822 13 633 2159 184 Cuts at: 9394 1305 Size: 2007 Fragments arranged by size: 2662 2159 2007 1431 822 633 185 184 13 MscI TGG'CCA Cuts at: 10 64 1489 1832 3291 4443 5264 10 Size: 54 1425 343 1459 1152 821 4842 Fragments arranged by size: 4842 1459 1425 1152 821 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2082 2490 3539 3681 4098 4218 Size: 648 843 73 408 1049 142 417 120 Cuts at: 4218 4309 5457 6078 6789 6966 8054 8413 8572 Size: 91 1148 621 711 177 1088 359 159 Cuts at: 8572 518 Size: 2042 Fragments arranged by size: 2042 1148 1088 1049 843 711 648 621 417 408 359 177 159 142 120 91 73 MspA1I CmG'CkG Cuts at: 1606 4436 7512 8189 9337 9582 1606 Size: 2830 3076 677 1148 245 2120 Fragments arranged by size: 3076 2830 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 8605 Fragments arranged by size: 8605 939 552 NdeI CA'TA_TG Cuts at: 387 2179 3016 3025 5127 5529 387 Size: 1792 837 9 2102 402 4954 Fragments arranged by size: 4954 2102 1792 837 402 9 NheI G'CTAG_C Cuts at: 1052 6004 1052 Size: 4952 5144 NotI GC'GGCC_GC Cuts at: 7543 7543 Size: 10096 NsiI A_TGCA'T Cuts at: 4273 4562 6963 4273 Size: 289 2401 7406 Fragments arranged by size: 7406 2401 289 NspI r_CATG'y Cuts at: 1132 1229 2489 4560 4619 4830 5098 5452 6045 Size: 97 1260 2071 59 211 268 354 593 Cuts at: 6045 6965 7397 7454 8999 1132 Size: 920 432 57 1545 2229 Fragments arranged by size: 2229 2071 1545 1260 920 593 432 354 268 211 97 59 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10096 PciI A'CATG_T Cuts at: 6041 7393 8995 6041 Size: 1352 1602 7142 Fragments arranged by size: 7142 1602 1352 PflMI CCAn_nnn'nTGG Cuts at: 1108 2615 1108 Size: 1507 8589 PfoI T'CCnGG_A Cuts at: 2560 5784 5812 6742 2560 Size: 3224 28 930 5914 Fragments arranged by size: 5914 3224 930 28 PmeI GTTT'AAAC Cuts at: 7400 7400 Size: 10096 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8165 8197 9678 9710 8165 Size: 32 1481 32 8551 Fragments arranged by size: 8551 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2564 2564 Size: 10096 PshAI GACnn'nnGTC Cuts at: 1613 6044 1613 Size: 4431 5665 PsiI TTA'TAA Cuts at: 4548 6803 7666 4548 Size: 2255 863 6978 Fragments arranged by size: 6978 2255 863 PspXI vC'TCGA_Gb Cuts at: 7091 7091 Size: 10096 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 6742 6774 6742 Size: 32 10064 PstI C_TGCA'G Cuts at: 830 6313 7448 830 Size: 5483 1135 3478 Fragments arranged by size: 5483 3478 1135 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10096 PvuII CAG'CTG Cuts at: 4436 4436 Size: 10096 SacI G_AGCT'C Cuts at: 4207 6280 6850 4207 Size: 2073 570 7453 Fragments arranged by size: 7453 2073 570 SalI G'TCGA_C Cuts at: 1936 7438 1936 Size: 5502 4594 SapI GCTCTTCn'nnn_ Cuts at: 5691 5914 5691 Size: 223 9873 SbfI CC_TGCA'GG Cuts at: 7448 7448 Size: 10096 ScaI AGT'ACT Cuts at: 4496 6507 8344 10056 4496 Size: 2011 1837 1712 4536 Fragments arranged by size: 4536 2011 1837 1712 SexAI A'CCwGG_T Cuts at: 5672 5672 Size: 10096 SfcI C'TryA_G Cuts at: 826 1045 4286 6309 6682 7172 7444 8579 9260 Size: 219 3241 2023 373 490 272 1135 681 Cuts at: 9260 9451 826 Size: 191 1471 Fragments arranged by size: 3241 2023 1471 1135 681 490 373 272 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10096 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10096 SmaI CCC'GGG Cuts at: 1872 7423 1872 Size: 5551 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3417 3500 3785 6443 7005 7091 Size: 197 926 1474 83 285 2658 562 86 Cuts at: 7091 7266 8200 9101 9363 820 Size: 175 934 901 262 1553 Fragments arranged by size: 2658 1553 1474 934 926 901 562 285 262 197 175 86 83 SnaBI TAC'GTA Cuts at: 493 7887 493 Size: 7394 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10096 SphI G_CATG'C Cuts at: 1132 4560 4830 5452 7454 1132 Size: 3428 270 622 2002 3774 Fragments arranged by size: 3774 3428 2002 622 270 SspI AAT'ATT Cuts at: 5 52 2705 8020 5 Size: 47 2653 5315 2081 Fragments arranged by size: 5315 2653 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 10096 StyI C'CwwG_G Cuts at: 513 1065 2004 2540 3286 4512 4961 7535 513 Size: 552 939 536 746 1226 449 2574 3074 Fragments arranged by size: 3074 2574 1226 939 746 552 536 449 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4505 8123 8140 8293 8478 8923 325 Size: 1255 2925 3618 17 153 185 445 1498 Fragments arranged by size: 3618 2925 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4346 4494 6505 Size: 274 80 33 51 1443 2369 148 2011 Cuts at: 6505 8342 10054 96 Size: 1837 1712 138 Fragments arranged by size: 2369 2011 1837 1712 1443 274 148 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 10056 10088 10056 Size: 32 10064 Tth111I GACn'n_nGTC Cuts at: 1316 3530 1316 Size: 2214 7882 XbaI T'CTAG_A Cuts at: 7207 7432 7207 Size: 225 9871 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4510 4510 Size: 10096 XhoI C'TCGA_G Cuts at: 1943 7091 1943 Size: 5148 4948 XmaI C'CCGG_G Cuts at: 1870 7421 1870 Size: 5551 4545 XmnI GAAnn'nnTTC Cuts at: 3222 3906 5487 6525 6999 8225 3222 Size: 684 1581 1038 474 1226 5093 Fragments arranged by size: 5093 1581 1226 1038 684 474 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7904 276 Size: 53 83 186 936 6370 2468 Fragments arranged by size: 6370 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI RsrII SacII SanDI SfiI SfoI SrfI SwaI