Restriction map for
pFN21AA1429
(Linear) (Six-base) MAPSORT of: KIAA1429.seq Check: 3436 from: 1 to: 5391
KIAA1429.orf
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AccI GT'mk_AC
Cuts at: 0 812 1295 3652 4896 4941 5391
Size: 812 483 2357 1244 45 450
Fragments arranged by size:
2357 1244 812 483 450 45
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 1001 1035 2918 3086 3338 3614 3929
Size: 977 24 34 1883 168 252 276 315
Cuts at: 3929 4507 4634 5391
Size: 578 127 757
Fragments arranged by size:
1883 977 757 578 315 276 252 168
127 34 24
AfeI AGC'GCT
Cuts at: 0 57 1331 3107 4711 5391
Size: 57 1274 1776 1604 680
Fragments arranged by size:
1776 1604 1274 680 57
AflII C'TTAA_G
Cuts at: 0 1495 4438 5391
Size: 1495 2943 953
Fragments arranged by size:
2943 1495 953
AflIII A'CryG_T
Cuts at: 0 2802 2909 4036 5391
Size: 2802 107 1127 1355
Fragments arranged by size:
2802 1355 1127 107
AleI CACnn'nnGTG
Cuts at: 0 2093 5391
Size: 2093 3298
AlwNI CAG_nnn'CTG
Cuts at: 0 610 1774 4449 4530 5391
Size: 610 1164 2675 81 861
Fragments arranged by size:
2675 1164 861 610 81
ApoI r'AATT_y
Cuts at: 0 675 899 1122 1217 1317 2184 3176 4195
Size: 675 224 223 95 100 867 992 1019
Cuts at: 4195 4208 4350 5391
Size: 13 142 1041
Fragments arranged by size:
1041 1019 992 867 675 224 223 142
100 95 13
AseI AT'TA_AT
Cuts at: 0 3737 4298 5391
Size: 3737 561 1093
Fragments arranged by size:
3737 1093 561
AvaI C'yCGr_G
Cuts at: 0 51 5086 5188 5391
Size: 51 5035 102 203
Fragments arranged by size:
5035 203 102 51
BamHI G'GATC_C
Cuts at: 0 3070 5391
Size: 3070 2321
BanII G_rGCy'C
Cuts at: 0 147 659 1413 2202 4275 4845 5391
Size: 147 512 754 789 2073 570 546
Fragments arranged by size:
2073 789 754 570 546 512 147
BbsI GAAGACnn'nnnn_
Cuts at: 0 989 1732 2143 2573 3620 5391
Size: 989 743 411 430 1047 1771
Fragments arranged by size:
1771 1047 989 743 430 411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1753 1787 5391
Size: 1753 34 3604
Fragments arranged by size:
3604 1753 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 962 5391
Size: 962 4429
BclI T'GATC_A
Cuts at: 0 1445 1476 1758 2217 3873 4575 5391
Size: 1445 31 282 459 1656 702 816
Fragments arranged by size:
1656 1445 816 702 459 282 31
BfrBI ATG'CAT
Cuts at: 0 2266 2555 4956 5391
Size: 2266 289 2401 435
Fragments arranged by size:
2401 2266 435 289
BglI GCCn_nnn'nGGC
Cuts at: 0 2961 5391
Size: 2961 2430
BglII A'GATC_T
Cuts at: 0 2370 2856 4629 5391
Size: 2370 486 1773 762
Fragments arranged by size:
2370 1773 762 486
BlpI GC'TnA_GC
Cuts at: 0 59 5391
Size: 59 5332
Bme1580I G_kGCm'C
Cuts at: 0 243 5391
Size: 243 5148
BmtI G_CTAG'C
Cuts at: 0 4003 5391
Size: 4003 1388
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3954 5391
Size: 3954 1437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 1970 2473 3830 3949 4471 4496 4721
Size: 539 1431 503 1357 119 522 25 225
Cuts at: 4721 5045 5391
Size: 324 346
Fragments arranged by size:
1431 1357 539 522 503 346 324 225
119 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1397 1801 4985 5246 5391
Size: 1397 404 3184 261 145
Fragments arranged by size:
3184 1397 404 261 145
BsaI GGTCTCn'nnnn_
Cuts at: 0 2242 3809 4258 4783 5391
Size: 2242 1567 449 525 608
Fragments arranged by size:
2242 1567 608 525 449
BsaAI yAC'GTr
Cuts at: 0 1482 2803 5391
Size: 1482 1321 2588
Fragments arranged by size:
2588 1482 1321
BsaBI GATnn'nnATC
Cuts at: 0 1832 4403 4628 5327 5391
Size: 1832 2571 225 699 64
Fragments arranged by size:
2571 1832 699 225 64
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1968 1998 3110 3140 3326 3356 3803 3833
Size: 1968 30 1112 30 186 30 447 30
Cuts at: 3833 5391
Size: 1558
Fragments arranged by size:
1968 1558 1112 447 186 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 542 623 841 2242 5074 5391
Size: 542 81 218 1401 2832 317
Fragments arranged by size:
2832 1401 542 317 218 81
BseYI C'CCAG_C
Cuts at: 0 446 1372 5391
Size: 446 926 4019
Fragments arranged by size:
4019 926 446
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2371 3006 3557 5391
Size: 2371 635 551 1834
Fragments arranged by size:
2371 1834 635 551
BsiHKAI G_wGCw'C
Cuts at: 0 2088 2202 3136 4122 4275 4845 5075 5391
Size: 2088 114 934 986 153 570 230 316
Fragments arranged by size:
2088 986 934 570 316 230 153 114
Bsp1286I G_dGCh'C
Cuts at: 0 147 243 659 1413 2088 2202 3136 4122
Size: 147 96 416 754 675 114 934 986
Cuts at: 4122 4275 4845 5075 5391
Size: 153 570 230 316
Fragments arranged by size:
986 934 754 675 570 416 316 230
153 147 114 96
BspHI T'CATG_A
Cuts at: 0 399 4905 5391
Size: 399 4506 486
Fragments arranged by size:
4506 486 399
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3514 4839 5391
Size: 3514 1325 552
Fragments arranged by size:
3514 1325 552
BsrDI GCAATG_nn'
Cuts at: 0 2299 2311 3367 3881 5391
Size: 2299 12 1056 514 1510
Fragments arranged by size:
2299 1510 1056 514 12
BsrGI T'GTAC_A
Cuts at: 0 2341 5391
Size: 2341 3050
BssSI C'ACGA_G
Cuts at: 0 5367 5391
Size: 5367 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3506 5391
Size: 3506 1885
BstEII G'GTnAC_C
Cuts at: 0 1708 2052 4462 5172 5391
Size: 1708 344 2410 710 219
Fragments arranged by size:
2410 1708 710 344 219
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2248 5391
Size: 2248 3143
BstYI r'GATC_y
Cuts at: 0 1833 2370 2856 3070 3783 4629 5391
Size: 1833 537 486 214 713 846 762
Fragments arranged by size:
1833 846 762 713 537 486 214
BstZ17I GTA'TAC
Cuts at: 0 3653 4897 5391
Size: 3653 1244 494
Fragments arranged by size:
3653 1244 494
Bsu36I CC'TnA_GG
Cuts at: 0 824 5391
Size: 824 4567
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 31 5391
Size: 31 5360
BtsI GCAGTG_nn'
Cuts at: 0 974 2792 5258 5294 5391
Size: 974 1818 2466 36 97
Fragments arranged by size:
2466 1818 974 97 36
ClaI AT'CG_AT
Cuts at: 0 2756 5391
Size: 2756 2635
DraI TTT'AAA
Cuts at: 0 45 1316 1512 4091 5391
Size: 45 1271 196 2579 1300
Fragments arranged by size:
2579 1300 1271 196 45
EaeI y'GGCC_r
Cuts at: 0 1284 2436 3257 5026 5391
Size: 1284 1152 821 1769 365
Fragments arranged by size:
1769 1284 1152 821 365
EarI CTCTTCn'nnn_
Cuts at: 0 617 716 773 827 839 854 1742 3038
Size: 617 99 57 54 12 15 888 1296
Cuts at: 3038 3242 3368 3686 3909 4526 4610 5146 5391
Size: 204 126 318 223 617 84 536 245
Fragments arranged by size:
1296 888 617 617 536 318 245 223
204 126 99 84 57 54 15 12
EcoICRI GAG'CTC
Cuts at: 0 2200 4273 4843 5391
Size: 2200 2073 570 548
Fragments arranged by size:
2200 2073 570 548
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 977 1001 1035 1970 2473 2918 3086
Size: 539 438 24 34 935 503 445 168
Cuts at: 3086 3338 3614 3830 3929 3949 4471 4496 4507
Size: 252 276 216 99 20 522 25 11
Cuts at: 4507 4634 4721 5045 5391
Size: 127 87 324 346
Fragments arranged by size:
935 539 522 503 445 438 346 324
276 252 216 168 127 99 87 34
25 24 20 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2840 5391
Size: 2840 2551
EcoO109I rG'GnC_Cy
Cuts at: 0 559 5136 5391
Size: 559 4577 255
Fragments arranged by size:
4577 559 255
EcoRI G'AATT_C
Cuts at: 0 675 5391
Size: 675 4716
EcoRV GAT'ATC
Cuts at: 0 2023 3189 5391
Size: 2023 1166 2202
Fragments arranged by size:
2202 2023 1166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1297 1348 3310 4668 5391
Size: 1297 51 1962 1358 723
Fragments arranged by size:
1962 1358 1297 723 51
HaeII r_GCGC'y
Cuts at: 0 59 1333 3109 4713 5391
Size: 59 1274 1776 1604 678
Fragments arranged by size:
1776 1604 1274 678 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 394 426 999 1031 1468 1500 1719 1751
Size: 394 32 573 32 437 32 219 32
Cuts at: 1751 1983 2015 2184 2216 2506 2538 3326 3358
Size: 232 32 169 32 290 32 788 32
Cuts at: 3358 3547 3579 4165 4197 4681 4713 5391
Size: 189 32 586 32 484 32 678
Fragments arranged by size:
788 678 586 573 484 437 394 290
232 219 189 169 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1154 2848 2973 3050 4650 5391
Size: 1154 1694 125 77 1600 741
Fragments arranged by size:
1694 1600 1154 741 125 77
HindIII A'AGCT_T
Cuts at: 0 1310 1514 1553 1597 1817 2629 3707 4372
Size: 1310 204 39 44 220 812 1078 665
Cuts at: 4372 5391
Size: 1019
Fragments arranged by size:
1310 1078 1019 812 665 220 204 44
39
HpaI GTT'AAC
Cuts at: 0 1154 3050 5391
Size: 1154 1896 2341
Fragments arranged by size:
2341 1896 1154
MfeI C'AATT_G
Cuts at: 0 1255 3547 4226 5391
Size: 1255 2292 679 1165
Fragments arranged by size:
2292 1255 1165 679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1962 2147 3578 4400 4413 5046 5391
Size: 1962 185 1431 822 13 633 345
Fragments arranged by size:
1962 1431 822 633 345 185 13
MscI TGG'CCA
Cuts at: 0 1286 2438 3259 5391
Size: 1286 1152 821 2132
Fragments arranged by size:
2132 1286 1152 821
MslI CAynn'nnrTG
Cuts at: 0 77 485 1534 1676 2093 2213 2304 3452
Size: 77 408 1049 142 417 120 91 1148
Cuts at: 3452 4073 4784 4961 5391
Size: 621 711 177 430
Fragments arranged by size:
1148 1049 711 621 430 417 408 177
142 120 91 77
MspA1I CmG'CkG
Cuts at: 0 2431 5391
Size: 2431 2960
NdeI CA'TA_TG
Cuts at: 0 174 1011 1020 3122 3524 5391
Size: 174 837 9 2102 402 1867
Fragments arranged by size:
2102 1867 837 402 174 9
NheI G'CTAG_C
Cuts at: 0 3999 5391
Size: 3999 1392
NsiI A_TGCA'T
Cuts at: 0 2268 2557 4958 5391
Size: 2268 289 2401 433
Fragments arranged by size:
2401 2268 433 289
NspI r_CATG'y
Cuts at: 0 484 2555 2614 2825 3093 3447 4040 4960
Size: 484 2071 59 211 268 354 593 920
Cuts at: 4960 5391
Size: 431
Fragments arranged by size:
2071 920 593 484 431 354 268 211
59
PciI A'CATG_T
Cuts at: 0 4036 5391
Size: 4036 1355
PflMI CCAn_nnn'nTGG
Cuts at: 0 610 5391
Size: 610 4781
PfoI T'CCnGG_A
Cuts at: 0 555 3779 3807 4737 5391
Size: 555 3224 28 930 654
Fragments arranged by size:
3224 930 654 555 28
PpuMI rG'GwC_Cy
Cuts at: 0 559 5391
Size: 559 4832
PshAI GACnn'nnGTC
Cuts at: 0 4039 5391
Size: 4039 1352
PsiI TTA'TAA
Cuts at: 0 2543 4798 5391
Size: 2543 2255 593
Fragments arranged by size:
2543 2255 593
PspXI vC'TCGA_Gb
Cuts at: 0 5086 5391
Size: 5086 305
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4737 4769 5391
Size: 4737 32 622
Fragments arranged by size:
4737 622 32
PstI C_TGCA'G
Cuts at: 0 4308 5391
Size: 4308 1083
PvuII CAG'CTG
Cuts at: 0 2431 5391
Size: 2431 2960
SacI G_AGCT'C
Cuts at: 0 2202 4275 4845 5391
Size: 2202 2073 570 546
Fragments arranged by size:
2202 2073 570 546
SapI GCTCTTCn'nnn_
Cuts at: 0 3686 3909 5391
Size: 3686 223 1482
Fragments arranged by size:
3686 1482 223
ScaI AGT'ACT
Cuts at: 0 2491 4502 5391
Size: 2491 2011 889
Fragments arranged by size:
2491 2011 889
SexAI A'CCwGG_T
Cuts at: 0 3667 5391
Size: 3667 1724
SfcI C'TryA_G
Cuts at: 0 2281 4304 4677 5167 5391
Size: 2281 2023 373 490 224
Fragments arranged by size:
2281 2023 490 373 224
SmlI C'TyrA_G
Cuts at: 0 1412 1495 1780 4438 5000 5086 5261 5391
Size: 1412 83 285 2658 562 86 175 130
Fragments arranged by size:
2658 1412 562 285 175 130 86 83
SphI G_CATG'C
Cuts at: 0 2555 2825 3447 5391
Size: 2555 270 622 1944
Fragments arranged by size:
2555 1944 622 270
SspI AAT'ATT
Cuts at: 0 700 5391
Size: 700 4691
StyI C'CwwG_G
Cuts at: 0 535 1281 2507 2956 5391
Size: 535 746 1226 449 2435
Fragments arranged by size:
2435 1226 746 535 449
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2500 5391
Size: 2500 2891
TatI w'GTAC_w
Cuts at: 0 2341 2489 4500 5391
Size: 2341 148 2011 891
Fragments arranged by size:
2341 2011 891 148
Tth111I GACn'n_nGTC
Cuts at: 0 1525 5391
Size: 1525 3866
XbaI T'CTAG_A
Cuts at: 0 5202 5391
Size: 5202 189
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2505 5391
Size: 2505 2886
XhoI C'TCGA_G
Cuts at: 0 5086 5391
Size: 5086 305
XmnI GAAnn'nnTTC
Cuts at: 0 1217 1901 3482 4520 4994 5391
Size: 1217 684 1581 1038 474 397
Fragments arranged by size:
1581 1217 1038 684 474 397
Enzymes that do cut:
AccI AcuI AfeI AflII AflIII AleI AlwNI ApoI
AseI AvaI BamHI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstAPI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII HindIII HpaI MfeI
MmeI MscI MslI MspA1I NdeI NheI NsiI NspI
PciI PflMI PfoI PpuMI PshAI PsiI PspXI PsrI
PstI PvuII SacI SapI ScaI SexAI SfcI SmlI
SphI SspI StyI TaqII TatI Tth111I XbaI XcmI
XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AgeI AhdI AlfI AloI
ApaI ApaLI AscI AsiSI AvrII BaeI BanI BbeI
BbvCI BmgBI BmrI Bpu10I BsaHI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BsrBI BsrFI BssHII BstBI BtgI
CspCI DraIII DrdI EagI EciI FseI FspI FspAI
KasI KpnI MluI NaeI NarI NcoI NgoMIV NotI
NruI PacI PasI PmeI PmlI PpiI PspOMI PvuI
RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TstI
XmaI ZraI