(Circular) (Six-base) MAPSORT of: pFN21ASDA1427.seq Check: 3698 from: 1 to: 6007 pFN21ASDA1427 6007 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2111 2362 2111 Size: 251 5756 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3817 278 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2333 3350 1937 Size: 396 1017 4594 Fragments arranged by size: 4594 1017 396 Acc65I G'GTAC_C Cuts at: 3328 3328 Size: 6007 AclI AA'CG_TT Cuts at: 1563 4134 4507 1563 Size: 2571 373 3063 Fragments arranged by size: 3063 2571 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2844 2873 3062 4070 5454 5722 1632 Size: 1212 29 189 1008 1384 268 1917 Fragments arranged by size: 1917 1384 1212 1008 268 189 29 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5810 AflIII A'CryG_T Cuts at: 1203 4906 5883 1203 Size: 3703 977 1327 Fragments arranged by size: 3703 1327 977 AgeI A'CCGG_T Cuts at: 4838 4838 Size: 6007 AhdI GACnn_n'nnGTC Cuts at: 4736 4736 Size: 6007 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6007 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3201 1184 Size: 2017 3990 AlwNI CAG_nnn'CTG Cuts at: 2885 3240 3750 5322 2885 Size: 355 510 1572 3570 Fragments arranged by size: 3570 1572 510 355 ApaLI G'TGCA_C Cuts at: 4064 5220 4064 Size: 1156 4851 ApoI r'AATT_y Cuts at: 1493 3316 3542 3694 5803 1493 Size: 1823 226 152 2109 1697 Fragments arranged by size: 2109 1823 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4561 160 Size: 872 3529 1606 Fragments arranged by size: 3529 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 AvaI C'yCGr_G Cuts at: 1870 1943 2189 3220 3332 1870 Size: 73 246 1031 112 4545 Fragments arranged by size: 4545 1031 246 112 73 BamHI G'GATC_C Cuts at: 3337 3704 3337 Size: 367 5640 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 2054 3285 3328 4783 Size: 325 217 627 248 19 1231 43 1455 Cuts at: 4783 618 Size: 1842 Fragments arranged by size: 1842 1455 1231 627 325 248 217 43 19 BanII G_rGCy'C Cuts at: 1391 2346 2724 2796 2988 3227 3326 1391 Size: 955 378 72 192 239 99 4072 Fragments arranged by size: 4072 955 378 239 192 99 72 BbeI G_GCGC'C Cuts at: 2058 2058 Size: 6007 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2861 928 Size: 971 962 4074 Fragments arranged by size: 4074 971 962 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3014 3048 4198 4232 1903 Size: 34 1077 34 1150 34 3678 Fragments arranged by size: 3678 1150 1077 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3899 5109 3899 Size: 1210 4797 BclI T'GATC_A Cuts at: 1548 3099 1548 Size: 1551 4456 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4618 136 Size: 107 122 71 631 605 2946 1525 Fragments arranged by size: 2946 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6002 6002 Size: 6007 BlpI GC'TnA_GC Cuts at: 3066 3424 3066 Size: 358 5649 Bme1580I G_kGCm'C Cuts at: 1790 2069 2248 4068 5224 1790 Size: 279 179 1820 1156 2573 Fragments arranged by size: 2573 1820 1156 279 179 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6007 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2833 3250 3738 Size: 416 53 83 399 24 1416 417 488 Cuts at: 3738 4696 442 Size: 958 1753 Fragments arranged by size: 1753 1416 958 488 417 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6007 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2186 2280 2907 3121 4667 1239 Size: 947 94 627 214 1546 2579 Fragments arranged by size: 2579 1546 947 627 214 94 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2237 2372 2770 4132 4997 5295 2237 Size: 135 398 1362 865 298 2949 Fragments arranged by size: 2949 1362 865 398 298 135 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2453 4670 882 Size: 617 954 2217 2219 Fragments arranged by size: 2219 2217 954 617 BsaAI yAC'GTr Cuts at: 493 1204 3304 3798 493 Size: 711 2100 494 2702 Fragments arranged by size: 2702 2100 711 494 BsaBI GATnn'nnATC Cuts at: 6001 6001 Size: 6007 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2055 3814 4196 4831 Size: 53 83 186 936 522 1759 382 635 Cuts at: 4831 275 Size: 1451 Fragments arranged by size: 1759 1451 936 635 522 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4440 4838 5112 5259 5670 4440 Size: 398 274 147 411 4777 Fragments arranged by size: 4777 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2753 2783 1178 Size: 30 165 30 1350 30 4402 Fragments arranged by size: 4402 1350 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2240 2354 2420 2702 2729 2732 3260 1186 Size: 1054 114 66 282 27 3 528 3933 Fragments arranged by size: 3933 1054 528 282 114 66 27 3 BseYI C'CCAG_C Cuts at: 2050 2062 2180 3157 5210 5706 2050 Size: 12 118 977 2053 496 2351 Fragments arranged by size: 2351 2053 977 496 118 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2181 2334 2657 3197 3278 1163 Size: 573 445 153 323 540 81 3892 Fragments arranged by size: 3892 573 540 445 323 153 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2764 3457 4218 5246 1540 Size: 275 186 763 693 761 1028 2301 Fragments arranged by size: 2301 1028 763 761 693 275 186 BsiHKAI G_wGCw'C Cuts at: 2703 2724 2796 2988 4068 4153 5224 2703 Size: 21 72 192 1080 85 1071 3486 Fragments arranged by size: 3486 1080 1071 192 85 72 21 BsmI GAATG_Cn' Cuts at: 3518 3611 3518 Size: 93 5914 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2248 2346 2703 2724 2796 2988 Size: 399 279 179 98 357 21 72 192 Cuts at: 2988 3227 3326 4068 4153 5224 1391 Size: 239 99 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 357 279 239 192 179 99 98 85 72 21 BspEI T'CCGG_A Cuts at: 5670 5670 Size: 6007 BspHI T'CATG_A Cuts at: 3896 5626 3896 Size: 1730 4277 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2111 2362 2386 2765 3132 3362 844 Size: 1267 251 24 379 367 230 3489 Fragments arranged by size: 3489 1267 379 367 251 230 24 BsrBI CCG'CTC Cuts at: 1394 1823 3894 1394 Size: 429 2071 3507 Fragments arranged by size: 3507 2071 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4502 4676 65 Size: 1183 3254 174 1396 Fragments arranged by size: 3254 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2630 3267 4651 4838 1700 Size: 110 820 637 1384 187 2869 Fragments arranged by size: 2869 1384 820 637 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6007 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6007 BssSI C'ACGA_G Cuts at: 4067 5079 4067 Size: 1012 4995 BstAPI GCAn_nnn'nTGC Cuts at: 794 3292 794 Size: 2498 3509 BstBI TT'CG_AA Cuts at: 2007 4811 2007 Size: 2804 3203 BstEII G'GTnAC_C Cuts at: 1184 2098 3013 1184 Size: 914 915 4178 Fragments arranged by size: 4178 915 914 BstYI r'GATC_y Cuts at: 1972 3337 3704 4089 4106 5558 5644 5656 6002 Size: 1365 367 385 17 1452 86 12 346 Cuts at: 6002 1972 Size: 1977 Fragments arranged by size: 1977 1452 1365 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2144 2223 513 Size: 552 957 122 79 4297 Fragments arranged by size: 4297 957 552 122 79 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5268 Fragments arranged by size: 5268 683 56 BtsI GCAGTG_nn' Cuts at: 2346 3196 3523 4317 4337 2346 Size: 850 327 794 20 4016 Fragments arranged by size: 4016 850 794 327 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6007 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5972 DraI TTT'AAA Cuts at: 3311 3311 Size: 6007 DraIII CAC_nnn'GTG Cuts at: 2782 2782 Size: 6007 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2603 5014 809 Size: 507 1287 2411 1802 Fragments arranged by size: 2411 1802 1287 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2619 2784 3193 Size: 54 1425 50 275 18 789 165 409 Cuts at: 3193 3454 4343 5972 8 Size: 261 889 1629 43 Fragments arranged by size: 1629 1425 889 789 409 275 261 165 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3454 1537 Size: 275 1642 4090 Fragments arranged by size: 4090 1642 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3937 1353 Size: 2584 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2726 2786 4594 4968 5114 387 Size: 1064 138 1137 60 1808 374 146 1280 Fragments arranged by size: 1808 1280 1137 1064 374 146 138 60 EcoICRI GAG'CTC Cuts at: 2722 2794 2986 2722 Size: 72 192 5743 Fragments arranged by size: 5743 192 72 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 2280 2844 2873 2907 3062 3121 Size: 393 554 94 564 29 34 155 59 Cuts at: 3121 4070 4667 5454 5722 1239 Size: 949 597 787 268 1524 Fragments arranged by size: 1524 949 787 597 564 554 393 268 155 94 59 34 29 EcoNI CCTnn'n_nnAGG Cuts at: 2168 2711 2993 3128 2168 Size: 543 282 135 5047 Fragments arranged by size: 5047 543 282 135 EcoO109I rG'GnC_Cy Cuts at: 2155 2497 2692 2754 2893 2155 Size: 342 195 62 139 5269 Fragments arranged by size: 5269 342 195 139 62 EcoRI G'AATT_C Cuts at: 3316 3694 3316 Size: 378 5629 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2200 3034 2200 Size: 834 5173 FspI TGC'GCA Cuts at: 4513 4513 Size: 6007 HaeII r_GCGC'y Cuts at: 1678 2058 5154 1678 Size: 380 3096 2531 Fragments arranged by size: 3096 2531 380 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2753 2785 2801 2833 4648 Size: 32 135 32 1181 32 16 32 1815 Cuts at: 4648 4680 4722 4754 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1815 1181 135 42 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 3351 3597 669 Size: 988 281 1413 246 3079 Fragments arranged by size: 3079 1413 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6007 HpaI GTT'AAC Cuts at: 3597 3597 Size: 6007 KasI G'GCGC_C Cuts at: 2054 2054 Size: 6007 KpnI G_GTAC'C Cuts at: 3332 3332 Size: 6007 MfeI C'AATT_G Cuts at: 3606 3606 Size: 6007 MluI A'CGCG_T Cuts at: 5883 5883 Size: 6007 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2468 5121 5305 1305 Size: 1163 2653 184 2007 Fragments arranged by size: 2653 2007 1163 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2621 3195 10 Size: 54 1425 343 789 574 2822 Fragments arranged by size: 2822 1425 789 574 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3965 4324 4483 518 Size: 648 2799 359 159 2042 Fragments arranged by size: 2799 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2146 2285 2671 3292 3423 4100 5248 5493 Size: 540 139 386 621 131 677 1148 245 Cuts at: 5493 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 621 540 386 245 139 131 NaeI GCC'GGC Cuts at: 3269 3269 Size: 6007 NarI GG'CG_CC Cuts at: 2055 2055 Size: 6007 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4498 Fragments arranged by size: 4498 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6007 NgoMIV G'CCGG_C Cuts at: 3267 3267 Size: 6007 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6007 NotI GC'GGCC_GC Cuts at: 3454 3454 Size: 6007 NspI r_CATG'y Cuts at: 1132 1229 2122 3365 4910 1132 Size: 97 893 1243 1545 2229 Fragments arranged by size: 2229 1545 1243 893 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6007 PciI A'CATG_T Cuts at: 4906 4906 Size: 6007 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6007 PmeI GTTT'AAAC Cuts at: 3311 3311 Size: 6007 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2396 2428 2951 2983 4076 4108 5589 5621 2396 Size: 32 523 32 1093 32 1481 32 2782 Fragments arranged by size: 2782 1481 1093 523 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2155 2754 2893 2155 Size: 599 139 5269 Fragments arranged by size: 5269 599 139 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6007 PsiI TTA'TAA Cuts at: 3577 3577 Size: 6007 PstI C_TGCA'G Cuts at: 830 2881 3132 3203 3359 830 Size: 2051 251 71 156 3478 Fragments arranged by size: 3478 2051 251 156 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6007 PvuII CAG'CTG Cuts at: 2285 2671 3292 2285 Size: 386 621 5000 Fragments arranged by size: 5000 621 386 SacI G_AGCT'C Cuts at: 2724 2796 2988 2724 Size: 72 192 5743 Fragments arranged by size: 5743 192 72 SacII CC_GC'GG Cuts at: 2147 2147 Size: 6007 SalI G'TCGA_C Cuts at: 1936 3349 1936 Size: 1413 4594 SbfI CC_TGCA'GG Cuts at: 3359 3359 Size: 6007 ScaI AGT'ACT Cuts at: 4255 5967 4255 Size: 1712 4295 SfcI C'TryA_G Cuts at: 826 1045 2877 3128 3199 3355 4490 5171 5362 Size: 219 1832 251 71 156 1135 681 191 Cuts at: 5362 826 Size: 1471 Fragments arranged by size: 1832 1471 1135 681 251 219 191 156 71 SfoI GGC'GCC Cuts at: 2056 2056 Size: 6007 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6007 SmaI CCC'GGG Cuts at: 1872 3334 1872 Size: 1462 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2252 2387 2749 4111 5012 5274 Size: 197 926 309 135 362 1362 901 262 Cuts at: 5274 820 Size: 1553 Fragments arranged by size: 1553 1362 926 901 362 309 262 197 135 SnaBI TAC'GTA Cuts at: 493 3304 3798 493 Size: 2811 494 2702 Fragments arranged by size: 2811 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6007 SphI G_CATG'C Cuts at: 1132 3365 1132 Size: 2233 3774 SspI AAT'ATT Cuts at: 5 52 3931 5 Size: 47 3879 2081 Fragments arranged by size: 3879 2081 47 StuI AGG'CCT Cuts at: 1517 3134 1517 Size: 1617 4390 StyI C'CwwG_G Cuts at: 513 1065 2022 2608 2979 3446 513 Size: 552 957 586 371 467 3074 Fragments arranged by size: 3074 957 586 552 467 371 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4034 4051 4204 4389 4834 325 Size: 1255 2454 17 153 185 445 1498 Fragments arranged by size: 2454 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4253 5965 96 Size: 274 80 33 51 1443 2276 1712 138 Fragments arranged by size: 2276 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5967 5999 5967 Size: 32 5975 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6007 XbaI T'CTAG_A Cuts at: 3343 3343 Size: 6007 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2932 2986 3145 2932 Size: 54 159 5794 Fragments arranged by size: 5794 159 54 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6007 XmaI C'CCGG_G Cuts at: 1870 3332 1870 Size: 1462 4545 XmnI GAAnn'nnTTC Cuts at: 2232 4136 2232 Size: 1904 4103 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3815 276 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI