(Circular) (Six-base) MAPSORT of: pFN21ASDA1412.seq Check: 316 from: 1 to: 8878 pFN21ASDA1412 8878 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5608 5608 Size: 8878 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6688 278 Size: 53 83 186 936 5152 2468 Fragments arranged by size: 5152 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3459 6221 1937 Size: 1522 2762 4594 Fragments arranged by size: 4594 2762 1522 Acc65I G'GTAC_C Cuts at: 6199 6199 Size: 8878 AclI AA'CG_TT Cuts at: 1563 7005 7378 1563 Size: 5442 373 3063 Fragments arranged by size: 5442 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2142 2235 2897 3588 6941 8325 8593 1632 Size: 510 93 662 691 3353 1384 268 1917 Fragments arranged by size: 3353 1917 1384 691 662 510 268 93 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8681 AflIII A'CryG_T Cuts at: 1203 4327 4789 7777 8754 1203 Size: 3124 462 2988 977 1327 Fragments arranged by size: 3124 2988 1327 977 462 AgeI A'CCGG_T Cuts at: 7709 7709 Size: 8878 AhdI GACnn_n'nnGTC Cuts at: 3736 7607 3736 Size: 3871 5007 AleI CACnn'nnGTG Cuts at: 1166 3438 3681 1166 Size: 2272 243 6363 Fragments arranged by size: 6363 2272 243 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2617 3788 5002 1184 Size: 1433 1171 1214 5060 Fragments arranged by size: 5060 1433 1214 1171 AlwNI CAG_nnn'CTG Cuts at: 3430 5451 5517 6621 8193 3430 Size: 2021 66 1104 1572 4115 Fragments arranged by size: 4115 2021 1572 1104 66 ApaI G_GGCC'C Cuts at: 4653 4653 Size: 8878 ApaLI G'TGCA_C Cuts at: 6935 8091 6935 Size: 1156 7722 ApoI r'AATT_y Cuts at: 1493 2165 2764 2856 3167 3277 3480 3595 4192 Size: 672 599 92 311 110 203 115 597 Cuts at: 4192 4414 4735 5375 6187 6413 6565 8674 1493 Size: 222 321 640 812 226 152 2109 1697 Fragments arranged by size: 2109 1697 812 672 640 599 597 321 311 226 222 203 152 115 110 92 AseI AT'TA_AT Cuts at: 160 1032 4047 5097 7432 160 Size: 872 3015 1050 2335 1606 Fragments arranged by size: 3015 2335 1606 1050 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8878 AvaI C'yCGr_G Cuts at: 1870 1943 2814 6203 1870 Size: 73 871 3389 4545 Fragments arranged by size: 4545 3389 871 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4696 4729 4696 Size: 33 8845 BamHI G'GATC_C Cuts at: 2392 2525 2935 5773 6208 6575 2392 Size: 133 410 2838 435 367 4695 Fragments arranged by size: 4695 2838 435 410 367 133 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5176 6199 7654 618 Size: 325 217 627 3389 1023 1455 1842 Fragments arranged by size: 3389 1842 1455 1023 627 325 217 BanII G_rGCy'C Cuts at: 1391 4653 6197 1391 Size: 3262 1544 4072 Fragments arranged by size: 4072 3262 1544 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2193 928 Size: 971 294 7613 Fragments arranged by size: 7613 971 294 BbvCI CC'TCA_GC Cuts at: 3471 3471 Size: 8878 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7069 7103 1903 Size: 34 5132 34 3678 Fragments arranged by size: 5132 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3701 4892 5076 6770 7980 3701 Size: 1191 184 1694 1210 4599 Fragments arranged by size: 4599 1694 1210 1191 184 BclI T'GATC_A Cuts at: 1548 1548 Size: 8878 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7489 136 Size: 107 122 71 631 605 5817 1525 Fragments arranged by size: 5817 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8873 8873 Size: 8878 BlpI GC'TnA_GC Cuts at: 6295 6295 Size: 8878 Bme1580I G_kGCm'C Cuts at: 1790 2728 4653 6939 8095 1790 Size: 938 1925 2286 1156 2573 Fragments arranged by size: 2573 2286 1925 1156 938 BmgBI CAC'GTC Cuts at: 1318 3691 1318 Size: 2373 6505 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3056 4119 6609 Size: 416 53 83 399 24 1639 1063 2490 Cuts at: 6609 7567 442 Size: 958 1753 Fragments arranged by size: 2490 1753 1639 1063 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8878 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2459 2459 Size: 8878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2331 2732 3330 4593 4680 5162 5374 7538 Size: 1092 401 598 1263 87 482 212 2164 Cuts at: 7538 1239 Size: 2579 Fragments arranged by size: 2579 2164 1263 1092 598 482 401 212 87 Bpu10I CC'TnA_GC Cuts at: 3471 3471 Size: 8878 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3742 7003 7868 8166 3742 Size: 3261 865 298 4454 Fragments arranged by size: 4454 3261 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3306 5694 6129 7541 882 Size: 617 1807 2388 435 1412 2219 Fragments arranged by size: 2388 2219 1807 1412 617 435 BsaAI yAC'GTr Cuts at: 493 1204 4328 6175 6669 493 Size: 711 3124 1847 494 2702 Fragments arranged by size: 3124 2702 1847 711 494 BsaBI GATnn'nnATC Cuts at: 2370 5064 8872 2370 Size: 2694 3808 2376 Fragments arranged by size: 3808 2694 2376 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2460 5754 6685 7067 Size: 53 83 186 936 927 3294 931 382 Cuts at: 7067 7702 275 Size: 635 1451 Fragments arranged by size: 3294 1451 936 931 927 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7311 7709 7983 8130 8541 7311 Size: 398 274 147 411 7648 Fragments arranged by size: 7648 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4371 4401 1178 Size: 30 165 30 2968 30 5655 Fragments arranged by size: 5655 2968 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2127 2130 3374 3881 4407 4410 5750 5913 Size: 941 3 1244 507 526 3 1340 163 Cuts at: 5913 1186 Size: 4151 Fragments arranged by size: 4151 1340 1244 941 526 507 163 3 3 BseYI C'CCAG_C Cuts at: 5691 8081 8577 5691 Size: 2390 496 5992 Fragments arranged by size: 5992 2390 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3416 5769 5800 1163 Size: 573 1680 2353 31 4241 Fragments arranged by size: 4241 2353 1680 573 31 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6328 7089 8117 1540 Size: 275 186 4327 761 1028 2301 Fragments arranged by size: 4327 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4673 5030 5088 5894 6939 7024 8095 4673 Size: 357 58 806 1045 85 1071 5456 Fragments arranged by size: 5456 1071 1045 806 357 85 58 BsmI GAATG_Cn' Cuts at: 2204 2470 3392 4981 5335 6389 6482 2204 Size: 266 922 1589 354 1054 93 4600 Fragments arranged by size: 4600 1589 1054 922 354 266 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2384 2384 Size: 8878 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2728 4653 4673 5030 5088 5894 6197 Size: 399 938 1925 20 357 58 806 303 Cuts at: 6197 6939 7024 8095 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1925 1071 938 806 742 399 357 303 85 58 20 BspEI T'CCGG_A Cuts at: 8541 8541 Size: 8878 BspHI T'CATG_A Cuts at: 4728 6767 8497 4728 Size: 2039 1730 5109 Fragments arranged by size: 5109 2039 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5608 6233 844 Size: 4764 625 3489 Fragments arranged by size: 4764 3489 625 BsrBI CCG'CTC Cuts at: 1394 1823 6765 1394 Size: 429 4942 3507 Fragments arranged by size: 4942 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2123 2902 3112 4353 7373 7547 65 Size: 1183 875 779 210 1241 3020 174 1396 Fragments arranged by size: 3020 1396 1241 1183 875 779 210 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5173 5575 7522 7709 1700 Size: 110 3363 402 1947 187 2869 Fragments arranged by size: 3363 2869 1947 402 187 110 BsrGI T'GTAC_A Cuts at: 96 5014 96 Size: 4918 3960 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8878 BssSI C'ACGA_G Cuts at: 4772 5055 6938 7950 4772 Size: 283 1883 1012 5700 Fragments arranged by size: 5700 1883 1012 283 BstAPI GCAn_nnn'nTGC Cuts at: 794 3102 794 Size: 2308 6570 BstBI TT'CG_AA Cuts at: 2007 7682 2007 Size: 5675 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8878 BstXI CCAn_nnnn'nTGG Cuts at: 2573 2860 5080 5168 5438 5992 2573 Size: 287 2220 88 270 554 5459 Fragments arranged by size: 5459 2220 554 287 270 88 BstYI r'GATC_y Cuts at: 1972 2392 2525 2935 5481 5773 6208 6575 6960 Size: 420 133 410 2546 292 435 367 385 Cuts at: 6960 6977 8429 8515 8527 8873 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2546 1977 1452 435 420 410 385 367 346 292 133 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2770 2770 Size: 8878 BtgI C'CryG_G Cuts at: 513 1065 5180 513 Size: 552 4115 4211 Fragments arranged by size: 4211 4115 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8139 Fragments arranged by size: 8139 683 56 BtsI GCAGTG_nn' Cuts at: 3145 4717 4986 4996 6394 7188 7208 3145 Size: 1572 269 10 1398 794 20 4815 Fragments arranged by size: 4815 1572 1398 794 269 20 10 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8878 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3708 3743 529 Size: 35 3144 35 5664 Fragments arranged by size: 5664 3144 35 35 DraI TTT'AAA Cuts at: 4363 5853 6013 6182 4363 Size: 1490 160 169 7059 Fragments arranged by size: 7059 1490 169 160 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4223 7885 809 Size: 507 2907 3662 1802 Fragments arranged by size: 3662 2907 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2850 3184 5440 Size: 54 1425 50 275 18 1020 334 2256 Cuts at: 5440 5523 5577 6325 7214 8843 8 Size: 83 54 748 889 1629 43 Fragments arranged by size: 2256 1629 1425 1020 889 748 334 275 83 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6325 1537 Size: 275 4513 4090 Fragments arranged by size: 4513 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3375 5838 6127 6808 1353 Size: 2022 2463 289 681 3423 Fragments arranged by size: 3423 2463 2022 681 289 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4931 7465 7839 7985 387 Size: 1064 138 3342 2534 374 146 1280 Fragments arranged by size: 3342 2534 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 2235 2331 2732 2897 3330 3588 Size: 393 510 93 96 401 165 433 258 Cuts at: 3588 4593 4680 5162 5374 6941 7538 8325 8593 Size: 1005 87 482 212 1567 597 787 268 Cuts at: 8593 1239 Size: 1524 Fragments arranged by size: 1567 1524 1005 787 597 510 482 433 401 393 268 258 212 165 96 93 87 EcoNI CCTnn'n_nnAGG Cuts at: 2564 2564 Size: 8878 EcoO109I rG'GnC_Cy Cuts at: 2757 2787 2818 4995 6055 2757 Size: 30 31 2177 1060 5580 Fragments arranged by size: 5580 2177 1060 31 30 EcoRI G'AATT_C Cuts at: 2764 3480 4735 6187 6565 2764 Size: 716 1255 1452 378 5077 Fragments arranged by size: 5077 1452 1255 716 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3117 4222 4689 5128 3117 Size: 1105 467 439 6867 Fragments arranged by size: 6867 1105 467 439 FspI TGC'GCA Cuts at: 7384 7384 Size: 8878 HaeII r_GCGC'y Cuts at: 1678 2582 8025 1678 Size: 904 5443 2531 Fragments arranged by size: 5443 2531 904 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2224 2256 3722 3754 4012 Size: 32 135 32 652 32 1466 32 258 Cuts at: 4012 4044 4116 4148 4371 4389 4403 4421 4847 Size: 32 72 32 223 18 14 18 426 Cuts at: 4847 4879 4930 4962 5054 5086 5466 5498 7519 Size: 32 51 32 92 32 380 32 2021 Cuts at: 7519 7551 7593 7625 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2021 1466 652 426 380 258 223 135 92 72 51 42 32 32 32 32 32 32 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 5206 6222 6468 669 Size: 988 281 3268 1016 246 3079 Fragments arranged by size: 3268 3079 1016 988 281 246 HindIII A'AGCT_T Cuts at: 748 2154 3060 4465 6156 748 Size: 1406 906 1405 1691 3470 Fragments arranged by size: 3470 1691 1406 1405 906 HpaI GTT'AAC Cuts at: 6468 6468 Size: 8878 KpnI G_GTAC'C Cuts at: 6203 6203 Size: 8878 MfeI C'AATT_G Cuts at: 4716 6477 4716 Size: 1761 7117 MluI A'CGCG_T Cuts at: 8754 8754 Size: 8878 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2089 2924 3281 3457 5097 7992 8176 1305 Size: 784 835 357 176 1640 2895 184 2007 Fragments arranged by size: 2895 2007 1640 835 784 357 184 176 MscI TGG'CCA Cuts at: 10 64 1489 1832 3186 5442 5525 10 Size: 54 1425 343 1354 2256 83 3363 Fragments arranged by size: 3363 2256 1425 1354 343 83 54 MslI CAynn'nnrTG Cuts at: 518 1166 3005 3138 3438 3681 3770 3980 4305 Size: 648 1839 133 300 243 89 210 325 Cuts at: 4305 5166 5436 5967 6836 7195 7354 518 Size: 861 270 531 869 359 159 2042 Fragments arranged by size: 2042 1839 869 861 648 531 359 325 300 270 243 210 159 133 89 MspA1I CmG'CkG Cuts at: 1606 4058 5290 6091 6294 6971 8119 8364 1606 Size: 2452 1232 801 203 677 1148 245 2120 Fragments arranged by size: 2452 2120 1232 1148 801 677 245 203 NaeI GCC'GGC Cuts at: 5175 5577 5175 Size: 402 8476 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8326 NdeI CA'TA_TG Cuts at: 387 387 Size: 8878 NgoMIV G'CCGG_C Cuts at: 5173 5575 5173 Size: 402 8476 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8878 NotI GC'GGCC_GC Cuts at: 6325 6325 Size: 8878 NspI r_CATG'y Cuts at: 1132 1229 3719 4793 6236 7781 1132 Size: 97 2490 1074 1443 1545 2229 Fragments arranged by size: 2490 2229 1545 1443 1074 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8878 PciI A'CATG_T Cuts at: 4789 7777 4789 Size: 2988 5890 PflMI CCAn_nnn'nTGG Cuts at: 1108 2672 3046 3322 3883 5163 1108 Size: 1564 374 276 561 1280 4823 Fragments arranged by size: 4823 1564 1280 561 374 276 PfoI T'CCnGG_A Cuts at: 2395 2565 2931 3012 2395 Size: 170 366 81 8261 Fragments arranged by size: 8261 366 170 81 PmeI GTTT'AAAC Cuts at: 6182 6182 Size: 8878 PmlI CAC'GTG Cuts at: 4328 4328 Size: 8878 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6947 6979 8460 8492 6947 Size: 32 1481 32 7333 Fragments arranged by size: 7333 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2757 2787 4995 2757 Size: 30 2208 6640 Fragments arranged by size: 6640 2208 30 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8878 PsiI TTA'TAA Cuts at: 3699 4173 6448 3699 Size: 474 2275 6129 Fragments arranged by size: 6129 2275 474 PspOMI G'GGCC_C Cuts at: 4649 4649 Size: 8878 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 6063 6095 6063 Size: 32 8846 PstI C_TGCA'G Cuts at: 830 4063 4991 6230 830 Size: 3233 928 1239 3478 Fragments arranged by size: 3478 3233 1239 928 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8878 PvuII CAG'CTG Cuts at: 4058 6091 4058 Size: 2033 6845 SalI G'TCGA_C Cuts at: 1936 6220 1936 Size: 4284 4594 SbfI CC_TGCA'GG Cuts at: 6230 6230 Size: 8878 ScaI AGT'ACT Cuts at: 5543 7126 8838 5543 Size: 1583 1712 5583 Fragments arranged by size: 5583 1712 1583 SfcI C'TryA_G Cuts at: 826 1045 2475 3052 4059 4987 5545 6226 7361 Size: 219 1430 577 1007 928 558 681 1135 Cuts at: 7361 8042 8233 826 Size: 681 191 1471 Fragments arranged by size: 1471 1430 1135 1007 928 681 681 577 558 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8878 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8878 SmaI CCC'GGG Cuts at: 1872 2816 6205 1872 Size: 944 3389 4545 Fragments arranged by size: 4545 3389 944 SmlI C'TyrA_G Cuts at: 820 1017 1943 3757 6982 7883 8145 820 Size: 197 926 1814 3225 901 262 1553 Fragments arranged by size: 3225 1814 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6175 6669 493 Size: 5682 494 2702 Fragments arranged by size: 5682 2702 494 SpeI A'CTAG_T Cuts at: 152 2288 5630 5932 152 Size: 2136 3342 302 3098 Fragments arranged by size: 3342 3098 2136 302 SphI G_CATG'C Cuts at: 1132 6236 1132 Size: 5104 3774 SspI AAT'ATT Cuts at: 5 52 2536 3496 6802 5 Size: 47 2484 960 3306 2081 Fragments arranged by size: 3306 2484 2081 960 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8878 StyI C'CwwG_G Cuts at: 513 1065 3040 3505 4256 5870 6058 6317 513 Size: 552 1975 465 751 1614 188 259 3074 Fragments arranged by size: 3074 1975 1614 751 552 465 259 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2106 3428 6905 6922 7075 7260 7705 Size: 1255 526 1322 3477 17 153 185 445 Cuts at: 7705 325 Size: 1498 Fragments arranged by size: 3477 1498 1322 1255 526 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5014 5541 7124 Size: 274 80 33 51 1443 3037 527 1583 Cuts at: 7124 8836 96 Size: 1712 138 Fragments arranged by size: 3037 1712 1583 1443 527 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3358 3390 8838 8870 3358 Size: 32 5448 32 3366 Fragments arranged by size: 5448 3366 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5595 1316 Size: 4279 4599 XbaI T'CTAG_A Cuts at: 6214 6214 Size: 8878 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2072 4880 2072 Size: 2808 6070 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8878 XmaI C'CCGG_G Cuts at: 1870 2814 6203 1870 Size: 944 3389 4545 Fragments arranged by size: 4545 3389 944 XmnI GAAnn'nnTTC Cuts at: 3097 7007 3097 Size: 3910 4968 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6686 276 Size: 53 83 186 936 5152 2468 Fragments arranged by size: 5152 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI