(Circular) (Six-base) MAPSORT of: pFN21ASDA1399.seq Check: 7893 from: 1 to: 5959 pFN21ASDA1399 5959 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2410 2410 Size: 5959 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3302 1937 Size: 1365 4594 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2189 4022 5406 5674 1632 Size: 557 1833 1384 268 1917 Fragments arranged by size: 1917 1833 1384 557 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 3150 3248 1166 Size: 1984 98 3877 Fragments arranged by size: 3877 1984 98 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3120 1184 Size: 1936 4023 AlwNI CAG_nnn'CTG Cuts at: 3702 5274 3702 Size: 1572 4387 ApaI G_GGCC'C Cuts at: 2300 3104 2300 Size: 804 5155 ApaLI G'TGCA_C Cuts at: 2581 4016 5172 2581 Size: 1435 1156 3368 Fragments arranged by size: 3368 1435 1156 ApoI r'AATT_y Cuts at: 1493 3268 3494 3646 5755 1493 Size: 1775 226 152 2109 1697 Fragments arranged by size: 2109 1775 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 2301 3284 1870 Size: 73 358 983 4545 Fragments arranged by size: 4545 983 358 73 BamHI G'GATC_C Cuts at: 3289 3656 3289 Size: 367 5592 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2187 2270 3080 3280 4735 Size: 325 217 627 400 83 810 200 1455 Cuts at: 4735 618 Size: 1842 Fragments arranged by size: 1842 1455 810 627 400 325 217 200 83 BanII G_rGCy'C Cuts at: 1391 2300 2325 2555 2756 3104 3278 1391 Size: 909 25 230 201 348 174 4072 Fragments arranged by size: 4072 909 348 230 201 174 25 BbeI G_GCGC'C Cuts at: 2191 2274 2191 Size: 83 5876 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2456 928 Size: 971 557 4431 Fragments arranged by size: 4431 971 557 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4150 4184 1903 Size: 34 2213 34 3678 Fragments arranged by size: 3678 2213 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 1548 Size: 5959 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4570 136 Size: 107 122 71 631 605 2898 1525 Fragments arranged by size: 2898 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2844 5954 2844 Size: 3110 2849 BlpI GC'TnA_GC Cuts at: 2414 2549 3376 2414 Size: 135 827 4997 Fragments arranged by size: 4997 827 135 Bme1580I G_kGCm'C Cuts at: 1790 2101 2300 2585 3104 4020 5176 1790 Size: 311 199 285 519 916 1156 2573 Fragments arranged by size: 2573 1156 916 519 311 285 199 BmgBI CAC'GTC Cuts at: 1318 2662 2875 1318 Size: 1344 213 4402 Fragments arranged by size: 4402 1344 213 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2386 2480 3690 Size: 416 53 83 399 24 969 94 1210 Cuts at: 3690 4648 442 Size: 958 1753 Fragments arranged by size: 1753 1210 969 958 416 399 94 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2311 2650 3011 4619 1239 Size: 1072 339 361 1608 2579 Fragments arranged by size: 2579 1608 1072 361 339 Bpu10I CC'TnA_GC Cuts at: 3093 3093 Size: 5959 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2120 2181 3205 4084 4949 5247 2120 Size: 61 1024 879 865 298 2832 Fragments arranged by size: 2832 1024 879 865 298 61 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2135 2894 3065 3181 4622 882 Size: 617 636 759 171 116 1441 2219 Fragments arranged by size: 2219 1441 759 636 617 171 116 BsaAI yAC'GTr Cuts at: 493 1204 3256 3750 493 Size: 711 2052 494 2702 Fragments arranged by size: 2702 2052 711 494 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2188 2271 3766 4148 Size: 53 83 186 936 655 83 1495 382 Cuts at: 4148 4783 275 Size: 635 1451 Fragments arranged by size: 1495 1451 936 655 635 382 186 83 83 53 BsaWI w'CCGG_w Cuts at: 4392 4790 5064 5211 5622 4392 Size: 398 274 147 411 4729 Fragments arranged by size: 4729 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5734 Fragments arranged by size: 5734 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2123 2184 2416 3022 3049 3083 1186 Size: 937 61 232 606 27 34 4062 Fragments arranged by size: 4062 937 606 232 61 34 27 BseYI C'CCAG_C Cuts at: 2101 2226 2386 5162 5658 2101 Size: 125 160 2776 496 2402 Fragments arranged by size: 2776 2402 496 160 125 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3152 1163 Size: 573 1416 3970 Fragments arranged by size: 3970 1416 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3409 4170 5198 1540 Size: 275 186 1408 761 1028 2301 Fragments arranged by size: 2301 1408 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2585 4020 4105 5176 2585 Size: 1435 85 1071 3368 Fragments arranged by size: 3368 1435 1071 85 BsmI GAATG_Cn' Cuts at: 3470 3563 3470 Size: 93 5866 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2101 2300 2325 2555 2585 2756 3104 Size: 399 311 199 25 230 30 171 348 Cuts at: 3104 3278 4020 4105 5176 1391 Size: 174 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 348 311 230 199 174 171 85 30 25 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 3193 3234 3848 5578 3193 Size: 41 614 1730 3574 Fragments arranged by size: 3574 1730 614 41 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2410 3314 844 Size: 1566 904 3489 Fragments arranged by size: 3489 1566 904 BsrBI CCG'CTC Cuts at: 1394 1823 2620 3846 1394 Size: 429 797 1226 3507 Fragments arranged by size: 3507 1226 797 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4454 4628 65 Size: 1183 3206 174 1396 Fragments arranged by size: 3206 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3116 4603 4790 1700 Size: 110 1306 1487 187 2869 Fragments arranged by size: 2869 1487 1306 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 4019 5031 4019 Size: 1012 4947 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5959 BstBI TT'CG_AA Cuts at: 2007 4763 2007 Size: 2756 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstYI r'GATC_y Cuts at: 1972 2844 3289 3656 4041 4058 5510 5596 5608 Size: 872 445 367 385 17 1452 86 12 Cuts at: 5608 5954 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 872 445 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3127 3127 Size: 5959 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5407 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2650 2752 487 Size: 683 56 1424 102 3694 Fragments arranged by size: 3694 1424 683 102 56 BtsI GCAGTG_nn' Cuts at: 2425 2699 2960 3475 4269 4289 2425 Size: 274 261 515 794 20 4095 Fragments arranged by size: 4095 794 515 274 261 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2331 2366 529 Size: 35 1767 35 4122 Fragments arranged by size: 4122 1767 35 35 DraI TTT'AAA Cuts at: 2929 3263 2929 Size: 334 5625 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2244 3118 3406 Size: 54 1425 50 275 18 414 874 288 Cuts at: 3406 4295 5924 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 874 414 288 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2162 2765 2843 3060 3889 1353 Size: 809 603 78 217 829 3423 Fragments arranged by size: 3423 829 809 603 217 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4546 4920 5066 387 Size: 1064 138 2957 374 146 1280 Fragments arranged by size: 2957 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2311 2650 3011 4022 4619 5406 Size: 393 557 122 339 361 1011 597 787 Cuts at: 5406 5674 1239 Size: 268 1524 Fragments arranged by size: 1524 1011 787 597 557 393 361 339 268 122 EcoO109I rG'GnC_Cy Cuts at: 2296 2297 2499 2906 3100 3101 3131 2296 Size: 1 202 407 194 1 30 5124 Fragments arranged by size: 5124 407 202 194 30 1 1 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2194 2194 Size: 5959 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 2191 2204 2274 2827 5106 1678 Size: 513 13 70 553 2279 2531 Fragments arranged by size: 2531 2279 553 513 70 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2443 2450 2475 2482 3216 Size: 32 135 32 871 7 25 7 734 Cuts at: 3216 3248 4600 4632 4674 4706 1373 Size: 32 1352 32 42 32 2626 Fragments arranged by size: 2626 1352 871 734 135 42 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2796 2947 3303 3549 669 Size: 988 281 858 151 356 246 3079 Fragments arranged by size: 3079 988 858 356 281 246 151 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KasI G'GCGC_C Cuts at: 2187 2270 2187 Size: 83 5876 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5073 5257 1305 Size: 3768 184 2007 Fragments arranged by size: 3768 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4137 Fragments arranged by size: 4137 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2873 3150 3248 3917 4276 4435 Size: 648 861 846 277 98 669 359 159 Cuts at: 4435 518 Size: 2042 Fragments arranged by size: 2042 861 846 669 648 359 277 159 98 MspA1I CmG'CkG Cuts at: 1606 2145 2562 3375 4052 5200 5445 1606 Size: 539 417 813 677 1148 245 2120 Fragments arranged by size: 2120 1148 813 677 539 417 245 NaeI GCC'GGC Cuts at: 3118 3118 Size: 5959 NarI GG'CG_CC Cuts at: 2188 2271 2188 Size: 83 5876 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5407 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NgoMIV G'CCGG_C Cuts at: 3116 3116 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 2155 3317 4862 1132 Size: 97 926 1162 1545 2229 Fragments arranged by size: 2229 1545 1162 926 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5959 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2499 2906 2499 Size: 407 5552 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PspOMI G'GGCC_C Cuts at: 2296 3100 2296 Size: 804 5155 PstI C_TGCA'G Cuts at: 830 2694 3311 830 Size: 1864 617 3478 Fragments arranged by size: 3478 1864 617 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SapI GCTCTTCn'nnn_ Cuts at: 2162 2162 Size: 5959 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SfcI C'TryA_G Cuts at: 826 1045 2690 3307 4442 5123 5314 826 Size: 219 1645 617 1135 681 191 1471 Fragments arranged by size: 1645 1471 1135 681 617 219 191 SfoI GGC'GCC Cuts at: 2189 2272 2189 Size: 83 5876 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2135 2196 3184 4063 4964 5226 Size: 197 926 192 61 988 879 901 262 Cuts at: 5226 820 Size: 1553 Fragments arranged by size: 1553 988 926 901 879 262 197 192 61 SnaBI TAC'GTA Cuts at: 493 3256 3750 493 Size: 2763 494 2702 Fragments arranged by size: 2763 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 2155 3317 1132 Size: 1023 1162 3774 Fragments arranged by size: 3774 1162 1023 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 3398 513 Size: 552 2333 3074 Fragments arranged by size: 3074 2333 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2468 2563 3056 3986 4003 4156 4341 Size: 1255 888 95 493 930 17 153 185 Cuts at: 4341 4786 325 Size: 445 1498 Fragments arranged by size: 1498 1255 930 888 493 445 185 153 95 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4205 5917 96 Size: 274 80 33 51 1443 2228 1712 138 Fragments arranged by size: 2228 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2668 2700 2864 2896 2898 2930 5919 5951 2668 Size: 32 164 32 2 32 2989 32 2676 Fragments arranged by size: 2989 2676 164 32 32 32 32 2 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 2174 4088 2174 Size: 1914 4045 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI