(Circular) (Six-base) MAPSORT of: pFN21ASDA1397.seq Check: 3078 from: 1 to: 6763 pFN21ASDA1397 6763 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2355 2392 2581 2355 Size: 37 189 6537 Fragments arranged by size: 6537 189 37 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3207 4573 278 Size: 53 83 186 936 1671 1366 2468 Fragments arranged by size: 2468 1671 1366 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4106 1937 Size: 2169 4594 Acc65I G'GTAC_C Cuts at: 4084 4084 Size: 6763 AclI AA'CG_TT Cuts at: 1563 4890 5263 1563 Size: 3327 373 3063 Fragments arranged by size: 3327 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2999 4826 6210 6478 1632 Size: 1367 1827 1384 268 1917 Fragments arranged by size: 1917 1827 1384 1367 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6566 AflIII A'CryG_T Cuts at: 1203 2534 5662 6639 1203 Size: 1331 3128 977 1327 Fragments arranged by size: 3128 1331 1327 977 AgeI A'CCGG_T Cuts at: 5594 5594 Size: 6763 AhdI GACnn_n'nnGTC Cuts at: 5492 5492 Size: 6763 AleI CACnn'nnGTG Cuts at: 1166 3041 1166 Size: 1875 4888 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6763 AlwNI CAG_nnn'CTG Cuts at: 2311 2569 2927 3160 4506 6078 2311 Size: 258 358 233 1346 1572 2996 Fragments arranged by size: 2996 1572 1346 358 258 233 ApaLI G'TGCA_C Cuts at: 2899 4820 5976 2899 Size: 1921 1156 3686 Fragments arranged by size: 3686 1921 1156 ApoI r'AATT_y Cuts at: 1493 2245 3842 3963 4072 4298 4450 6559 1493 Size: 752 1597 121 109 226 152 2109 1697 Fragments arranged by size: 2109 1697 1597 752 226 152 121 109 AseI AT'TA_AT Cuts at: 160 1032 3981 5317 160 Size: 872 2949 1336 1606 Fragments arranged by size: 2949 1606 1336 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6763 AvaI C'yCGr_G Cuts at: 1870 1943 2812 3690 4088 1870 Size: 73 869 878 398 4545 Fragments arranged by size: 4545 878 869 398 73 BamHI G'GATC_C Cuts at: 4093 4460 4093 Size: 367 6396 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4084 5539 618 Size: 325 217 627 2297 1455 1842 Fragments arranged by size: 2297 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2301 3708 3752 4082 1391 Size: 910 1407 44 330 4072 Fragments arranged by size: 4072 1407 910 330 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3792 3985 928 Size: 971 1893 193 3706 Fragments arranged by size: 3706 1893 971 193 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4954 4988 1903 Size: 34 3017 34 3678 Fragments arranged by size: 3678 3017 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4655 5865 4655 Size: 1210 5553 BclI T'GATC_A Cuts at: 1548 1548 Size: 6763 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5374 136 Size: 107 122 71 631 605 3702 1525 Fragments arranged by size: 3702 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6758 6758 Size: 6763 BlpI GC'TnA_GC Cuts at: 4180 4180 Size: 6763 Bme1580I G_kGCm'C Cuts at: 1790 2903 3432 4824 5980 1790 Size: 1113 529 1392 1156 2573 Fragments arranged by size: 2573 1392 1156 1113 529 BmgBI CAC'GTC Cuts at: 1318 2632 3822 1318 Size: 1314 1190 4259 Fragments arranged by size: 4259 1314 1190 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2765 2887 3604 Size: 416 53 83 399 24 1348 122 717 Cuts at: 3604 4494 5452 442 Size: 890 958 1753 Fragments arranged by size: 1753 1348 958 890 717 416 399 122 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3633 1056 Size: 2577 4186 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3428 3553 3607 3905 5423 1239 Size: 2189 125 54 298 1518 2579 Fragments arranged by size: 2579 2189 1518 298 125 54 Bpu10I CC'TnA_GC Cuts at: 2869 3699 3813 2869 Size: 830 114 5819 Fragments arranged by size: 5819 830 114 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2421 4888 5753 6051 2421 Size: 2467 865 298 3133 Fragments arranged by size: 3133 2467 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5426 882 Size: 617 3927 2219 Fragments arranged by size: 3927 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2319 2418 2535 3121 4060 4554 493 Size: 711 1115 99 117 586 939 494 2702 Fragments arranged by size: 2702 1115 939 711 586 494 117 99 BsaBI GATnn'nnATC Cuts at: 6757 6757 Size: 6763 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3204 4570 4952 5587 Size: 53 83 186 936 1671 1366 382 635 Cuts at: 5587 275 Size: 1451 Fragments arranged by size: 1671 1451 1366 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5196 5594 5868 6015 6426 5196 Size: 398 274 147 411 5533 Fragments arranged by size: 5533 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3630 3660 1178 Size: 30 165 30 2227 30 4281 Fragments arranged by size: 4281 2227 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2193 1186 Size: 1007 5756 BseYI C'CCAG_C Cuts at: 2927 2946 3069 3755 5966 6462 2927 Size: 19 123 686 2211 496 3228 Fragments arranged by size: 3228 2211 686 496 123 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2149 2795 3065 3326 3331 3909 1163 Size: 573 413 646 270 261 5 578 4017 Fragments arranged by size: 4017 646 578 573 413 270 261 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2392 2583 4213 4974 6002 1540 Size: 275 186 391 191 1630 761 1028 2301 Fragments arranged by size: 2301 1630 1028 761 391 275 191 186 BsiHKAI G_wGCw'C Cuts at: 2903 3021 3138 3708 4824 4909 5980 2903 Size: 118 117 570 1116 85 1071 3686 Fragments arranged by size: 3686 1116 1071 570 118 117 85 BsmI GAATG_Cn' Cuts at: 2625 4274 4367 2625 Size: 1649 93 5021 Fragments arranged by size: 5021 1649 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2128 2128 Size: 6763 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2301 2903 3021 3138 3432 3708 3752 Size: 399 511 602 118 117 294 276 44 Cuts at: 3752 4082 4824 4909 5980 1391 Size: 330 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 602 511 399 330 294 276 118 117 85 44 BspEI T'CCGG_A Cuts at: 6426 6426 Size: 6763 BspHI T'CATG_A Cuts at: 4652 6382 4652 Size: 1730 5033 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2355 2392 2581 4118 844 Size: 1511 37 189 1537 3489 Fragments arranged by size: 3489 1537 1511 189 37 BsrBI CCG'CTC Cuts at: 1394 1823 2868 3337 4650 1394 Size: 429 1045 469 1313 3507 Fragments arranged by size: 3507 1313 1045 469 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5258 5432 65 Size: 1183 4010 174 1396 Fragments arranged by size: 4010 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3326 5407 5594 1700 Size: 110 1516 2081 187 2869 Fragments arranged by size: 2869 2081 1516 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6763 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6763 BssSI C'ACGA_G Cuts at: 2642 3041 4823 5835 2642 Size: 399 1782 1012 3570 Fragments arranged by size: 3570 1782 1012 399 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6763 BstBI TT'CG_AA Cuts at: 2007 5567 2007 Size: 3560 3203 BstEII G'GTnAC_C Cuts at: 1184 3074 1184 Size: 1890 4873 BstYI r'GATC_y Cuts at: 1972 3286 4093 4460 4845 4862 6314 6400 6412 Size: 1314 807 367 385 17 1452 86 12 Cuts at: 6412 6758 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1314 807 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2163 2392 2611 2856 2988 3488 3719 Size: 552 1098 229 219 245 132 500 231 Cuts at: 3719 3926 3969 513 Size: 207 43 3307 Fragments arranged by size: 3307 1098 552 500 245 231 229 219 207 132 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2337 3160 487 Size: 683 56 1111 823 4090 Fragments arranged by size: 4090 1111 823 683 56 BtsI GCAGTG_nn' Cuts at: 2040 2065 3249 3343 4279 5073 5093 2040 Size: 25 1184 94 936 794 20 3710 Fragments arranged by size: 3710 1184 936 794 94 25 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6763 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2992 3027 529 Size: 35 2428 35 4265 Fragments arranged by size: 4265 2428 35 35 DraI TTT'AAA Cuts at: 4067 4067 Size: 6763 DraIII CAC_nnn'GTG Cuts at: 3142 3142 Size: 6763 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3203 5770 809 Size: 507 1887 2567 1802 Fragments arranged by size: 2567 1887 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2160 2313 2580 Size: 54 1425 50 275 18 330 153 267 Cuts at: 2580 3464 4210 5099 6728 8 Size: 884 746 889 1629 43 Fragments arranged by size: 1629 1425 889 884 746 330 275 267 153 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2580 4210 1537 Size: 275 768 1630 4090 Fragments arranged by size: 4090 1630 768 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2975 4693 1353 Size: 1622 1718 3423 Fragments arranged by size: 3423 1718 1622 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5350 5724 5870 387 Size: 1064 138 3761 374 146 1280 Fragments arranged by size: 3761 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3706 3706 Size: 6763 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2999 3428 3553 3607 3905 4826 5423 Size: 393 1367 429 125 54 298 921 597 Cuts at: 5423 6210 6478 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1367 921 787 597 429 393 298 268 125 54 EcoNI CCTnn'n_nnAGG Cuts at: 2693 2693 Size: 6763 EcoRI G'AATT_C Cuts at: 4072 4450 4072 Size: 378 6385 FspI TGC'GCA Cuts at: 3734 5269 3734 Size: 1535 5228 HaeII r_GCGC'y Cuts at: 1678 3052 5910 1678 Size: 1374 2858 2531 Fragments arranged by size: 2858 2531 1374 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2705 2737 2834 2866 3294 Size: 32 135 32 1133 32 97 32 428 Cuts at: 3294 3326 3928 3960 5404 5436 5478 5510 1373 Size: 32 602 32 1444 32 42 32 2626 Fragments arranged by size: 2626 1444 1133 602 428 135 97 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2386 4107 4353 669 Size: 988 281 448 1721 246 3079 Fragments arranged by size: 3079 1721 988 448 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6763 HpaI GTT'AAC Cuts at: 4353 4353 Size: 6763 KpnI G_GTAC'C Cuts at: 4088 4088 Size: 6763 MfeI C'AATT_G Cuts at: 4362 4362 Size: 6763 MluI A'CGCG_T Cuts at: 6639 6639 Size: 6763 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2091 5877 6061 1305 Size: 786 3786 184 2007 Fragments arranged by size: 3786 2007 786 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2162 2315 10 Size: 54 1425 343 330 153 4458 Fragments arranged by size: 4458 1425 343 330 153 54 MslI CAynn'nnrTG Cuts at: 518 1166 2342 2771 3041 4721 5080 5239 518 Size: 648 1176 429 270 1680 359 159 2042 Fragments arranged by size: 2042 1680 1176 648 429 359 270 159 MspA1I CmG'CkG Cuts at: 1606 2394 3157 3490 3552 3928 4179 4856 6004 Size: 788 763 333 62 376 251 677 1148 Cuts at: 6004 6249 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 788 763 677 376 333 251 245 62 NaeI GCC'GGC Cuts at: 3328 3328 Size: 6763 NcoI C'CATG_G Cuts at: 513 1065 2988 3969 513 Size: 552 1923 981 3307 Fragments arranged by size: 3307 1923 981 552 NdeI CA'TA_TG Cuts at: 387 2543 387 Size: 2156 4607 NgoMIV G'CCGG_C Cuts at: 3326 3326 Size: 6763 NheI G'CTAG_C Cuts at: 1052 3629 1052 Size: 2577 4186 NotI GC'GGCC_GC Cuts at: 4210 4210 Size: 6763 NspI r_CATG'y Cuts at: 1132 1229 2668 4121 5666 1132 Size: 97 1439 1453 1545 2229 Fragments arranged by size: 2229 1545 1453 1439 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6763 PciI A'CATG_T Cuts at: 5662 5662 Size: 6763 PflMI CCAn_nnn'nTGG Cuts at: 1108 2311 3069 1108 Size: 1203 758 4802 Fragments arranged by size: 4802 1203 758 PfoI T'CCnGG_A Cuts at: 2084 2669 2084 Size: 585 6178 PmeI GTTT'AAAC Cuts at: 4067 4067 Size: 6763 PmlI CAC'GTG Cuts at: 2319 2418 2319 Size: 99 6664 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4832 4864 6345 6377 4832 Size: 32 1481 32 5218 Fragments arranged by size: 5218 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2500 1613 Size: 887 5876 PsiI TTA'TAA Cuts at: 4333 4333 Size: 6763 PstI C_TGCA'G Cuts at: 830 3156 4115 830 Size: 2326 959 3478 Fragments arranged by size: 3478 2326 959 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6763 PvuII CAG'CTG Cuts at: 3157 3552 3157 Size: 395 6368 SacI G_AGCT'C Cuts at: 3708 3708 Size: 6763 SacII CC_GC'GG Cuts at: 2395 3491 3929 2395 Size: 1096 438 5229 Fragments arranged by size: 5229 1096 438 SalI G'TCGA_C Cuts at: 1936 4105 1936 Size: 2169 4594 SbfI CC_TGCA'GG Cuts at: 4115 4115 Size: 6763 ScaI AGT'ACT Cuts at: 2230 5011 6723 2230 Size: 2781 1712 2270 Fragments arranged by size: 2781 2270 1712 SexAI A'CCwGG_T Cuts at: 4033 4033 Size: 6763 SfcI C'TryA_G Cuts at: 826 1045 3152 4111 5246 5927 6118 826 Size: 219 2107 959 1135 681 191 1471 Fragments arranged by size: 2107 1471 1135 959 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6763 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6763 SmaI CCC'GGG Cuts at: 1872 3692 4090 1872 Size: 1820 398 4545 Fragments arranged by size: 4545 1820 398 SmlI C'TyrA_G Cuts at: 820 1017 1943 2436 4867 5768 6030 820 Size: 197 926 493 2431 901 262 1553 Fragments arranged by size: 2431 1553 926 901 493 262 197 SnaBI TAC'GTA Cuts at: 493 4060 4554 493 Size: 3567 494 2702 Fragments arranged by size: 3567 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6763 SphI G_CATG'C Cuts at: 1132 4121 1132 Size: 2989 3774 SspI AAT'ATT Cuts at: 5 52 4687 5 Size: 47 4635 2081 Fragments arranged by size: 4635 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6763 StyI C'CwwG_G Cuts at: 513 1065 2988 3650 3969 4202 513 Size: 552 1923 662 319 233 3074 Fragments arranged by size: 3074 1923 662 552 319 233 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3655 3694 4790 4807 4960 5145 5590 Size: 1255 2075 39 1096 17 153 185 445 Cuts at: 5590 325 Size: 1498 Fragments arranged by size: 2075 1498 1255 1096 445 185 153 39 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2228 5009 6721 Size: 274 80 33 51 1443 251 2781 1712 Cuts at: 6721 96 Size: 138 Fragments arranged by size: 2781 1712 1443 274 251 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2080 2112 2489 2521 3128 3160 3630 3662 6723 Size: 32 377 32 607 32 470 32 3061 Cuts at: 6723 6755 2080 Size: 32 2088 Fragments arranged by size: 3061 2088 607 470 377 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2654 3308 1316 Size: 1338 654 4771 Fragments arranged by size: 4771 1338 654 XbaI T'CTAG_A Cuts at: 4099 4099 Size: 6763 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6763 XmaI C'CCGG_G Cuts at: 1870 3690 4088 1870 Size: 1820 398 4545 Fragments arranged by size: 4545 1820 398 XmnI GAAnn'nnTTC Cuts at: 4892 4892 Size: 6763 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3205 4571 276 Size: 53 83 186 936 1671 1366 2468 Fragments arranged by size: 2468 1671 1366 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SfiI SfoI SrfI SwaI XcmI