(Circular) (Six-base) MAPSORT of: pFN21ASDA1394.seq Check: 7461 from: 1 to: 7627 pFN21ASDA1394 7627 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3085 3280 4100 4686 3085 Size: 195 820 586 6026 Fragments arranged by size: 6026 820 586 195 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5437 278 Size: 53 83 186 936 3901 2468 Fragments arranged by size: 3901 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2262 4970 1937 Size: 325 2708 4594 Fragments arranged by size: 4594 2708 325 Acc65I G'GTAC_C Cuts at: 4072 4948 4072 Size: 876 6751 AclI AA'CG_TT Cuts at: 1563 5754 6127 1563 Size: 4191 373 3063 Fragments arranged by size: 4191 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5690 7074 7342 1632 Size: 4058 1384 268 1917 Fragments arranged by size: 4058 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7430 AflIII A'CryG_T Cuts at: 1203 6526 7503 1203 Size: 5323 977 1327 Fragments arranged by size: 5323 1327 977 AgeI A'CCGG_T Cuts at: 6458 6458 Size: 7627 AhdI GACnn_n'nnGTC Cuts at: 6356 6356 Size: 7627 AleI CACnn'nnGTG Cuts at: 1166 4731 1166 Size: 3565 4062 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2414 4250 1184 Size: 1230 1836 4561 Fragments arranged by size: 4561 1836 1230 AlwNI CAG_nnn'CTG Cuts at: 2728 2943 3628 4678 4687 4723 4867 5370 6942 Size: 215 685 1050 9 36 144 503 1572 Cuts at: 6942 2728 Size: 3413 Fragments arranged by size: 3413 1572 1050 685 503 215 144 36 9 ApaI G_GGCC'C Cuts at: 2144 4884 2144 Size: 2740 4887 ApaLI G'TGCA_C Cuts at: 4064 5684 6840 4064 Size: 1620 1156 4851 Fragments arranged by size: 4851 1620 1156 ApoI r'AATT_y Cuts at: 1493 4936 5162 5314 7423 1493 Size: 3443 226 152 2109 1697 Fragments arranged by size: 3443 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6181 160 Size: 872 5149 1606 Fragments arranged by size: 5149 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7627 AvaI C'yCGr_G Cuts at: 1870 1943 2096 2231 2247 2431 2486 4176 4952 Size: 73 153 135 16 184 55 1690 776 Cuts at: 4952 1870 Size: 4545 Fragments arranged by size: 4545 1690 776 184 153 135 73 55 16 AvrII C'CTAG_G Cuts at: 2815 2815 Size: 7627 BamHI G'GATC_C Cuts at: 2083 2225 4545 4957 5324 2083 Size: 142 2320 412 367 4386 Fragments arranged by size: 4386 2320 412 367 142 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2196 2332 2476 3222 3390 Size: 325 217 627 409 136 144 746 168 Cuts at: 3390 3565 4072 4259 4384 4771 4948 6403 618 Size: 175 507 187 125 387 177 1455 1842 Fragments arranged by size: 1842 1455 746 627 507 409 387 325 217 187 177 175 168 144 136 125 BanII G_rGCy'C Cuts at: 1391 2120 2144 2159 2384 2432 3693 3826 4473 Size: 729 24 15 225 48 1261 133 647 Cuts at: 4473 4557 4794 4884 4946 1391 Size: 84 237 90 62 4072 Fragments arranged by size: 4072 1261 729 647 237 225 133 90 84 62 48 24 15 BbeI G_GCGC'C Cuts at: 2200 3394 3569 2200 Size: 1194 175 6258 Fragments arranged by size: 6258 1194 175 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4450 928 Size: 971 2551 4105 Fragments arranged by size: 4105 2551 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3643 3677 5818 5852 1903 Size: 34 1706 34 2141 34 3678 Fragments arranged by size: 3678 2141 1706 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5519 6729 5519 Size: 1210 6417 BclI T'GATC_A Cuts at: 1548 1548 Size: 7627 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2115 2788 3338 Size: 107 122 71 631 605 443 673 550 Cuts at: 3338 3350 3554 4378 4851 6238 136 Size: 12 204 824 473 1387 1525 Fragments arranged by size: 1525 1387 824 673 631 605 550 473 443 204 122 107 71 12 BglII A'GATC_T Cuts at: 7622 7622 Size: 7627 BlpI GC'TnA_GC Cuts at: 2682 3176 3942 5044 2682 Size: 494 766 1102 5265 Fragments arranged by size: 5265 1102 766 494 Bme1580I G_kGCm'C Cuts at: 1790 2030 2144 2337 4023 4068 4262 4884 5688 Size: 240 114 193 1686 45 194 622 804 Cuts at: 5688 6844 1790 Size: 1156 2573 Fragments arranged by size: 2573 1686 1156 804 622 240 194 193 114 45 BmgBI CAC'GTC Cuts at: 1318 2206 4273 1318 Size: 888 2067 4672 Fragments arranged by size: 4672 2067 888 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2123 3259 3452 Size: 416 53 83 399 24 706 1136 193 Cuts at: 3452 3730 3868 5358 6316 442 Size: 278 138 1490 958 1753 Fragments arranged by size: 1753 1490 1136 958 706 416 399 278 193 138 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7627 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 2432 2720 3047 3056 3269 3377 3488 Size: 911 282 288 327 9 213 108 111 Cuts at: 3488 3512 3530 3677 4055 4118 4571 4778 6287 Size: 24 18 147 378 63 453 207 1509 Cuts at: 6287 1239 Size: 2579 Fragments arranged by size: 2579 1509 911 453 378 327 288 282 213 207 147 111 108 63 24 18 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3818 4457 5752 6617 6915 3818 Size: 639 1295 865 298 4530 Fragments arranged by size: 4530 1295 865 639 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3572 4703 6290 882 Size: 617 2073 1131 1587 2219 Fragments arranged by size: 2219 2073 1587 1131 617 BsaAI yAC'GTr Cuts at: 493 1204 4631 4924 5418 493 Size: 711 3427 293 494 2702 Fragments arranged by size: 3427 2702 711 494 293 BsaBI GATnn'nnATC Cuts at: 7621 7621 Size: 7627 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2197 2971 3391 3566 Size: 53 83 186 936 664 774 420 175 Cuts at: 3566 3648 4653 5434 5816 6451 275 Size: 82 1005 781 382 635 1451 Fragments arranged by size: 1451 1005 936 781 774 664 635 420 382 186 175 83 82 53 BsaWI w'CCGG_w Cuts at: 2308 3777 6060 6458 6732 6879 7290 2308 Size: 1469 2283 398 274 147 411 2645 Fragments arranged by size: 2645 2283 1469 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3053 3083 3215 3245 3503 Size: 30 165 30 1650 30 132 30 258 Cuts at: 3503 3533 1178 Size: 30 5272 Fragments arranged by size: 5272 1650 258 165 132 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3053 3056 3059 3296 3803 4775 4802 1186 Size: 1867 3 3 237 507 972 27 4011 Fragments arranged by size: 4011 1867 972 507 237 27 3 3 BseYI C'CCAG_C Cuts at: 2387 2733 2992 3081 3535 3922 3960 4216 4221 Size: 346 259 89 454 387 38 256 5 Cuts at: 4221 4487 4671 4820 4849 4883 6830 7326 2387 Size: 266 184 149 29 34 1947 496 2688 Fragments arranged by size: 2688 1947 496 454 387 346 266 259 256 184 149 89 38 34 29 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2960 3018 3683 3950 4134 4709 1163 Size: 573 1224 58 665 267 184 575 4081 Fragments arranged by size: 4081 1224 665 575 573 267 184 58 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3327 3441 3549 3596 4732 5077 Size: 275 186 1326 114 108 47 1136 345 Cuts at: 5077 5838 6866 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1326 1136 1028 761 345 275 186 114 108 47 BsiHKAI G_wGCw'C Cuts at: 3777 3879 4068 4161 4269 4473 4557 4636 5688 Size: 102 189 93 108 204 84 79 1052 Cuts at: 5688 5773 6844 3777 Size: 85 1071 4560 Fragments arranged by size: 4560 1071 1052 204 189 108 102 93 85 84 79 BsmI GAATG_Cn' Cuts at: 5138 5231 5138 Size: 93 7534 BsmBI CGTCTCn'nnnn_ Cuts at: 2852 4349 4844 2852 Size: 1497 495 5635 Fragments arranged by size: 5635 1497 495 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2120 2144 2159 2337 2384 2432 Size: 399 240 90 24 15 178 47 48 Cuts at: 2432 3693 3777 3826 3879 4023 4068 4161 4262 Size: 1261 84 49 53 144 45 93 101 Cuts at: 4262 4269 4473 4557 4636 4794 4884 4946 5688 Size: 7 204 84 79 158 90 62 742 Cuts at: 5688 5773 6844 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1261 1071 742 399 240 204 178 158 144 101 93 90 90 85 84 84 79 62 53 49 48 47 45 24 15 7 BspEI T'CCGG_A Cuts at: 2308 7290 2308 Size: 4982 2645 BspHI T'CATG_A Cuts at: 4089 5516 7246 4089 Size: 1427 1730 4470 Fragments arranged by size: 4470 1730 1427 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2669 2911 3085 3280 4100 4542 4578 4686 Size: 1825 242 174 195 820 442 36 108 Cuts at: 4686 4982 844 Size: 296 3489 Fragments arranged by size: 3489 1825 820 442 296 242 195 174 108 36 BsrBI CCG'CTC Cuts at: 1394 1823 5514 1394 Size: 429 3691 3507 Fragments arranged by size: 3691 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4234 6122 6296 65 Size: 1183 2986 1888 174 1396 Fragments arranged by size: 2986 1888 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2706 3362 3440 3541 6271 6458 1700 Size: 110 896 656 78 101 2730 187 2869 Fragments arranged by size: 2869 2730 896 656 187 110 101 78 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7627 BssHII G'CGCG_C Cuts at: 1925 3504 3599 3677 1925 Size: 1579 95 78 5875 Fragments arranged by size: 5875 1579 95 78 BssSI C'ACGA_G Cuts at: 3704 4635 5687 6699 3704 Size: 931 1052 1012 4632 Fragments arranged by size: 4632 1052 1012 931 BstAPI GCAn_nnn'nTGC Cuts at: 794 3973 4723 794 Size: 3179 750 3698 Fragments arranged by size: 3698 3179 750 BstBI TT'CG_AA Cuts at: 2007 6431 2007 Size: 4424 3203 BstEII G'GTnAC_C Cuts at: 1184 2362 1184 Size: 1178 6449 BstXI CCAn_nnnn'nTGG Cuts at: 2128 2741 3937 4381 2128 Size: 613 1196 444 5374 Fragments arranged by size: 5374 1196 613 444 BstYI r'GATC_y Cuts at: 1972 2083 2225 4545 4957 5324 5709 5726 7178 Size: 111 142 2320 412 367 385 17 1452 Cuts at: 7178 7264 7276 7622 1972 Size: 86 12 346 1977 Fragments arranged by size: 2320 1977 1452 412 385 367 346 142 111 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2323 2981 3202 3530 3982 4732 513 Size: 552 1258 658 221 328 452 750 3408 Fragments arranged by size: 3408 1258 750 658 552 452 328 221 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6888 Fragments arranged by size: 6888 683 56 BtsI GCAGTG_nn' Cuts at: 3006 3170 4122 4807 5143 5937 5957 3006 Size: 164 952 685 336 794 20 4676 Fragments arranged by size: 4676 952 794 685 336 164 20 ClaI AT'CG_AT Cuts at: 1554 4875 1554 Size: 3321 4306 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7592 DraI TTT'AAA Cuts at: 4931 4931 Size: 7627 DraIII CAC_nnn'GTG Cuts at: 3161 3706 4273 3161 Size: 545 567 6515 Fragments arranged by size: 6515 567 545 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6634 809 Size: 507 5318 1802 Fragments arranged by size: 5318 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2326 2620 3123 Size: 54 1425 50 275 18 496 294 503 Cuts at: 3123 3324 3339 3438 3546 4639 5074 5963 7592 Size: 201 15 99 108 1093 435 889 1629 Cuts at: 7592 8 Size: 43 Fragments arranged by size: 1629 1425 1093 889 503 496 435 294 275 201 108 99 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 3324 3438 3546 5074 1537 Size: 275 1512 114 108 1528 4090 Fragments arranged by size: 4090 1528 1512 275 114 108 EarI CTCTTCn'nnn_ Cuts at: 1353 2130 3944 5557 1353 Size: 777 1814 1613 3423 Fragments arranged by size: 3423 1814 1613 777 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2372 4832 6214 6588 6734 387 Size: 1064 138 783 2460 1382 374 146 1280 Fragments arranged by size: 2460 1382 1280 1064 783 374 146 138 EcoICRI GAG'CTC Cuts at: 4471 4555 4471 Size: 84 7543 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2432 2720 3047 3056 3269 3377 Size: 393 518 282 288 327 9 213 108 Cuts at: 3377 3488 3512 3530 3677 4055 4118 4571 4778 Size: 111 24 18 147 378 63 453 207 Cuts at: 4778 5690 6287 7074 7342 1239 Size: 912 597 787 268 1524 Fragments arranged by size: 1524 912 787 597 518 453 393 378 327 288 282 268 213 207 147 111 108 63 24 18 9 EcoNI CCTnn'n_nnAGG Cuts at: 3059 3278 3611 4859 3059 Size: 219 333 1248 5827 Fragments arranged by size: 5827 1248 333 219 EcoO109I rG'GnC_Cy Cuts at: 2093 2125 2140 2141 2545 2615 2902 4684 4720 Size: 32 15 1 404 70 287 1782 36 Cuts at: 4720 4765 4864 2093 Size: 45 99 4856 Fragments arranged by size: 4856 1782 404 287 99 70 45 36 32 15 1 EcoRI G'AATT_C Cuts at: 4936 5314 4936 Size: 378 7249 EcoRV GAT'ATC Cuts at: 2914 2914 Size: 7627 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2321 2321 Size: 7627 FseI GG_CCGG'CC Cuts at: 3366 3366 Size: 7627 FspI TGC'GCA Cuts at: 6133 6133 Size: 7627 HaeII r_GCGC'y Cuts at: 1678 2200 2950 3023 3260 3394 3569 3642 6774 Size: 522 750 73 237 134 175 73 3132 Cuts at: 6774 1678 Size: 2531 Fragments arranged by size: 3132 2531 750 522 237 175 134 73 73 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 6268 6300 6342 6374 1373 Size: 32 135 32 4696 32 42 32 2626 Fragments arranged by size: 4696 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4567 4971 5217 669 Size: 988 281 2629 404 246 3079 Fragments arranged by size: 3079 2629 988 404 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7627 HpaI GTT'AAC Cuts at: 5217 5217 Size: 7627 KasI G'GCGC_C Cuts at: 2196 3390 3565 2196 Size: 1194 175 6258 Fragments arranged by size: 6258 1194 175 KpnI G_GTAC'C Cuts at: 4076 4952 4076 Size: 876 6751 MfeI C'AATT_G Cuts at: 5226 5226 Size: 7627 MluI A'CGCG_T Cuts at: 7503 7503 Size: 7627 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3131 4344 6741 6925 1305 Size: 1826 1213 2397 184 2007 Fragments arranged by size: 2397 2007 1826 1213 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4641 10 Size: 54 1425 343 2809 2996 Fragments arranged by size: 2996 2809 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3981 4731 5585 5944 6103 518 Size: 648 2815 750 854 359 159 2042 Fragments arranged by size: 2815 2042 854 750 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2325 2983 3085 3204 3437 3532 3571 3625 Size: 719 658 102 119 233 95 39 54 Cuts at: 3625 3697 3926 3964 4425 5043 5720 6868 7113 Size: 72 229 38 461 618 677 1148 245 Cuts at: 7113 1606 Size: 2120 Fragments arranged by size: 2120 1148 719 677 658 618 461 245 233 229 119 102 95 72 54 39 38 NaeI GCC'GGC Cuts at: 3364 3442 3543 3364 Size: 78 101 7448 Fragments arranged by size: 7448 101 78 NarI GG'CG_CC Cuts at: 2197 3391 3566 2197 Size: 1194 175 6258 Fragments arranged by size: 6258 1194 175 NcoI C'CATG_G Cuts at: 513 1065 3982 513 Size: 552 2917 4158 Fragments arranged by size: 4158 2917 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7627 NgoMIV G'CCGG_C Cuts at: 3362 3440 3541 3362 Size: 78 101 7448 Fragments arranged by size: 7448 101 78 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7627 NotI GC'GGCC_GC Cuts at: 5074 5074 Size: 7627 NspI r_CATG'y Cuts at: 1132 1229 2443 4372 4985 6530 1132 Size: 97 1214 1929 613 1545 2229 Fragments arranged by size: 2229 1929 1545 1214 613 97 PasI CC'CwG_GG Cuts at: 1348 2145 2540 2905 1348 Size: 797 395 365 6070 Fragments arranged by size: 6070 797 395 365 PciI A'CATG_T Cuts at: 6526 6526 Size: 7627 PflMI CCAn_nnn'nTGG Cuts at: 1108 2206 4211 4648 4867 1108 Size: 1098 2005 437 219 3868 Fragments arranged by size: 3868 2005 1098 437 219 PfoI T'CCnGG_A Cuts at: 2556 4166 4548 2556 Size: 1610 382 5635 Fragments arranged by size: 5635 1610 382 PmeI GTTT'AAAC Cuts at: 4931 4931 Size: 7627 PmlI CAC'GTG Cuts at: 4631 4631 Size: 7627 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5696 5728 7209 7241 5696 Size: 32 1481 32 6082 Fragments arranged by size: 6082 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2093 2125 2093 Size: 32 7595 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7627 PsiI TTA'TAA Cuts at: 5197 5197 Size: 7627 PspOMI G'GGCC_C Cuts at: 2140 4880 2140 Size: 2740 4887 PstI C_TGCA'G Cuts at: 830 2283 2295 2922 3917 4683 4719 4802 4979 Size: 1453 12 627 995 766 36 83 177 Cuts at: 4979 830 Size: 3478 Fragments arranged by size: 3478 1453 995 766 627 177 83 36 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7627 PvuII CAG'CTG Cuts at: 3085 3625 3697 3926 3964 3085 Size: 540 72 229 38 6748 Fragments arranged by size: 6748 540 229 72 38 SacI G_AGCT'C Cuts at: 4473 4557 4473 Size: 84 7543 SacII CC_GC'GG Cuts at: 2326 2984 3205 3533 2326 Size: 658 221 328 6420 Fragments arranged by size: 6420 658 328 221 SalI G'TCGA_C Cuts at: 1936 4969 1936 Size: 3033 4594 SanDI GG'GwC_CC Cuts at: 2093 2093 Size: 7627 SapI GCTCTTCn'nnn_ Cuts at: 3944 3944 Size: 7627 SbfI CC_TGCA'GG Cuts at: 2922 4683 4719 4979 2922 Size: 1761 36 260 5570 Fragments arranged by size: 5570 1761 260 36 ScaI AGT'ACT Cuts at: 5875 7587 5875 Size: 1712 5915 SexAI A'CCwGG_T Cuts at: 4068 4278 4068 Size: 210 7417 SfcI C'TryA_G Cuts at: 826 1045 2269 2279 2291 2918 3913 4679 4715 Size: 219 1224 10 12 627 995 766 36 Cuts at: 4715 4798 4975 6110 6791 6982 826 Size: 83 177 1135 681 191 1471 Fragments arranged by size: 1471 1224 1135 995 766 681 627 219 191 177 83 36 12 10 SfiI GGCCn_nnn'nGGCC Cuts at: 3338 3338 Size: 7627 SfoI GGC'GCC Cuts at: 2198 3392 3567 2198 Size: 1194 175 6258 Fragments arranged by size: 6258 1194 175 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7627 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7627 SmaI CCC'GGG Cuts at: 1872 2249 2433 2488 4954 1872 Size: 377 184 55 2466 4545 Fragments arranged by size: 4545 2466 377 184 55 SmlI C'TyrA_G Cuts at: 820 1017 1943 3833 4472 5731 6632 6894 820 Size: 197 926 1890 639 1259 901 262 1553 Fragments arranged by size: 1890 1553 1259 926 901 639 262 197 SnaBI TAC'GTA Cuts at: 493 4924 5418 493 Size: 4431 494 2702 Fragments arranged by size: 4431 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7627 SphI G_CATG'C Cuts at: 1132 4372 4985 1132 Size: 3240 613 3774 Fragments arranged by size: 3774 3240 613 SrfI GCCC'GGGC Cuts at: 2249 2249 Size: 7627 SspI AAT'ATT Cuts at: 5 52 5551 5 Size: 47 5499 2081 Fragments arranged by size: 5499 2081 47 StuI AGG'CCT Cuts at: 1517 2604 1517 Size: 1087 6540 StyI C'CwwG_G Cuts at: 513 1065 2121 2520 2815 3982 5066 513 Size: 552 1056 399 295 1167 1084 3074 Fragments arranged by size: 3074 1167 1084 1056 552 399 295 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2897 2984 3974 4109 4218 4511 5654 Size: 1255 1317 87 990 135 109 293 1143 Cuts at: 5654 5671 5824 6009 6454 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1317 1255 1143 990 445 293 185 153 135 109 87 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5873 7585 96 Size: 274 80 33 51 1443 3896 1712 138 Fragments arranged by size: 3896 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2332 2364 3145 3177 3215 3247 3503 3535 3716 Size: 32 781 32 38 32 256 32 181 Cuts at: 3716 3748 7587 7619 2332 Size: 32 3839 32 2340 Fragments arranged by size: 3839 2340 781 256 181 38 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2567 1316 Size: 1251 6376 XbaI T'CTAG_A Cuts at: 4963 4963 Size: 7627 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2593 4208 4240 2029 Size: 564 1615 32 5416 Fragments arranged by size: 5416 1615 564 32 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7627 XmaI C'CCGG_G Cuts at: 1870 2247 2431 2486 4952 1870 Size: 377 184 55 2466 4545 Fragments arranged by size: 4545 2466 377 184 55 XmnI GAAnn'nnTTC Cuts at: 5756 5756 Size: 7627 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5435 276 Size: 53 83 186 936 3901 2468 Fragments arranged by size: 3901 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I FspAI NruI NsiI PacI PspXI PsrI RsrII SwaI