(Circular) (Six-base) MAPSORT of: pFN21ASDA1392.seq Check: 5703 from: 1 to: 7507 pFN21ASDA1392 7507 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4222 4222 Size: 7507 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5317 278 Size: 53 83 186 936 3781 2468 Fragments arranged by size: 3781 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4850 1937 Size: 2913 4594 Acc65I G'GTAC_C Cuts at: 2493 4828 2493 Size: 2335 5172 AclI AA'CG_TT Cuts at: 1563 5634 6007 1563 Size: 4071 373 3063 Fragments arranged by size: 4071 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2919 3582 4059 5570 6954 7222 1632 Size: 1287 663 477 1511 1384 268 1917 Fragments arranged by size: 1917 1511 1384 1287 663 477 268 AfeI AGC'GCT Cuts at: 3727 3727 Size: 7507 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7310 AflIII A'CryG_T Cuts at: 1203 6406 7383 1203 Size: 5203 977 1327 Fragments arranged by size: 5203 1327 977 AgeI A'CCGG_T Cuts at: 2520 6338 2520 Size: 3818 3689 AhdI GACnn_n'nnGTC Cuts at: 3454 3639 4061 6236 3454 Size: 185 422 2175 4725 Fragments arranged by size: 4725 2175 422 185 AleI CACnn'nnGTG Cuts at: 1166 3305 4074 1166 Size: 2139 769 4599 Fragments arranged by size: 4599 2139 769 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2063 4092 1184 Size: 879 2029 4599 Fragments arranged by size: 4599 2029 879 AlwNI CAG_nnn'CTG Cuts at: 5250 6822 5250 Size: 1572 5935 ApaI G_GGCC'C Cuts at: 4016 4016 Size: 7507 ApaLI G'TGCA_C Cuts at: 2530 2639 3422 5564 6720 2530 Size: 109 783 2142 1156 3317 Fragments arranged by size: 3317 2142 1156 783 109 ApoI r'AATT_y Cuts at: 1493 2364 2755 2816 4472 4816 5042 5194 7303 Size: 871 391 61 1656 344 226 152 2109 Cuts at: 7303 1493 Size: 1697 Fragments arranged by size: 2109 1697 1656 871 391 344 226 152 61 AseI AT'TA_AT Cuts at: 160 1032 6061 160 Size: 872 5029 1606 Fragments arranged by size: 5029 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7507 AvaI C'yCGr_G Cuts at: 1870 1943 2243 2546 2943 3311 3758 4439 4611 Size: 73 300 303 397 368 447 681 172 Cuts at: 4611 4832 1870 Size: 221 4545 Fragments arranged by size: 4545 681 447 397 368 303 300 221 172 73 BamHI G'GATC_C Cuts at: 4837 5204 4837 Size: 367 7140 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2493 2722 3238 3859 4076 Size: 325 217 627 706 229 516 621 217 Cuts at: 4076 4421 4828 6283 618 Size: 345 407 1455 1842 Fragments arranged by size: 1842 1455 706 627 621 516 407 345 325 229 217 217 BanII G_rGCy'C Cuts at: 1391 3655 4016 4086 4682 4734 4826 1391 Size: 2264 361 70 596 52 92 4072 Fragments arranged by size: 4072 2264 596 361 92 70 52 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2036 2640 3558 4214 928 Size: 971 137 604 918 656 4221 Fragments arranged by size: 4221 971 918 656 604 137 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2262 2296 2589 2623 5698 5732 1903 Size: 34 325 34 293 34 3075 34 3678 Fragments arranged by size: 3678 3075 325 293 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2505 5399 6609 2505 Size: 2894 1210 3403 Fragments arranged by size: 3403 2894 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7507 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3976 6118 136 Size: 107 122 71 631 605 2304 2142 1525 Fragments arranged by size: 2304 2142 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2406 7502 2406 Size: 5096 2411 BlpI GC'TnA_GC Cuts at: 4924 4924 Size: 7507 Bme1580I G_kGCm'C Cuts at: 1790 2534 2643 3243 3426 3981 4016 5568 6724 Size: 744 109 600 183 555 35 1552 1156 Cuts at: 6724 1790 Size: 2573 Fragments arranged by size: 2573 1552 1156 744 600 555 183 109 35 BmgBI CAC'GTC Cuts at: 1318 2638 1318 Size: 1320 6187 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2881 5238 6196 Size: 416 53 83 399 24 1464 2357 958 Cuts at: 6196 442 Size: 1753 Fragments arranged by size: 2357 1753 1464 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7507 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3123 3123 Size: 7507 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2403 3946 6167 1239 Size: 1164 1543 2221 2579 Fragments arranged by size: 2579 2221 1543 1164 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5632 6497 6795 5632 Size: 865 298 6344 Fragments arranged by size: 6344 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2529 3141 3159 6170 882 Size: 617 1030 612 18 3011 2219 Fragments arranged by size: 3011 2219 1030 617 612 18 BsaAI yAC'GTr Cuts at: 493 1204 2331 4607 4804 5298 493 Size: 711 1127 2276 197 494 2702 Fragments arranged by size: 2702 2276 1127 711 494 197 BsaBI GATnn'nnATC Cuts at: 2960 7501 2960 Size: 4541 2966 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3545 4057 5314 5696 Size: 53 83 186 936 2012 512 1257 382 Cuts at: 5696 6331 275 Size: 635 1451 Fragments arranged by size: 2012 1451 1257 936 635 512 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2520 2835 3450 5940 6338 6612 6759 7170 2520 Size: 315 615 2490 398 274 147 411 2857 Fragments arranged by size: 2857 2490 615 411 398 315 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7282 Fragments arranged by size: 7282 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3067 3949 4001 4044 4181 4409 4412 4427 Size: 1881 882 52 43 137 228 3 15 Cuts at: 4427 1186 Size: 4266 Fragments arranged by size: 4266 1881 882 228 137 52 43 15 3 BseYI C'CCAG_C Cuts at: 3196 3590 4085 4293 6710 7206 3196 Size: 394 495 208 2417 496 3497 Fragments arranged by size: 3497 2417 496 495 394 208 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2114 3612 1163 Size: 573 378 1498 5058 Fragments arranged by size: 5058 1498 573 378 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4957 5718 6746 1540 Size: 275 186 2956 761 1028 2301 Fragments arranged by size: 2956 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2042 2322 2534 2643 3312 3426 3855 5568 5653 Size: 280 212 109 669 114 429 1713 85 Cuts at: 5653 6724 2042 Size: 1071 2825 Fragments arranged by size: 2825 1713 1071 669 429 280 212 114 109 85 BsmI GAATG_Cn' Cuts at: 5018 5111 5018 Size: 93 7414 BsmBI CGTCTCn'nnnn_ Cuts at: 2910 3839 4354 2910 Size: 929 515 6063 Fragments arranged by size: 6063 929 515 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2322 2534 2643 3243 3312 3426 Size: 399 252 280 212 109 600 69 114 Cuts at: 3426 3655 3855 3981 4016 4086 4682 4734 4826 Size: 229 200 126 35 70 596 52 92 Cuts at: 4826 5568 5653 6724 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 600 596 399 280 252 229 212 200 126 114 109 92 85 70 69 52 35 BspEI T'CCGG_A Cuts at: 7170 7170 Size: 7507 BspHI T'CATG_A Cuts at: 3021 5396 7126 3021 Size: 2375 1730 3402 Fragments arranged by size: 3402 2375 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3937 4222 4862 844 Size: 3093 285 640 3489 Fragments arranged by size: 3489 3093 640 285 BsrBI CCG'CTC Cuts at: 1394 1823 4444 5394 1394 Size: 429 2621 950 3507 Fragments arranged by size: 3507 2621 950 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2280 2591 4223 6002 6176 65 Size: 1183 1032 311 1632 1779 174 1396 Fragments arranged by size: 1779 1632 1396 1183 1032 311 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2520 6151 6338 1700 Size: 110 710 3631 187 2869 Fragments arranged by size: 3631 2869 710 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7507 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7507 BssSI C'ACGA_G Cuts at: 5567 6579 5567 Size: 1012 6495 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7507 BstBI TT'CG_AA Cuts at: 2007 6311 2007 Size: 4304 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7507 BstXI CCAn_nnnn'nTGG Cuts at: 2869 3502 4073 4093 2869 Size: 633 571 20 6283 Fragments arranged by size: 6283 633 571 20 BstYI r'GATC_y Cuts at: 1972 2250 2406 3470 3491 4837 5204 5589 5606 Size: 278 156 1064 21 1346 367 385 17 Cuts at: 5606 7058 7144 7156 7502 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1346 1064 385 367 346 278 156 86 21 17 12 Bsu36I CC'TnA_GG Cuts at: 2569 2569 Size: 7507 BtgI C'CryG_G Cuts at: 513 1065 2145 2991 3121 4072 4687 513 Size: 552 1080 846 130 951 615 3333 Fragments arranged by size: 3333 1080 951 846 615 552 130 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6768 Fragments arranged by size: 6768 683 56 BtsI GCAGTG_nn' Cuts at: 2605 2620 4436 5023 5817 5837 2605 Size: 15 1816 587 794 20 4275 Fragments arranged by size: 4275 1816 794 587 20 15 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7507 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7472 DraI TTT'AAA Cuts at: 2848 4811 2848 Size: 1963 5544 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3154 6514 809 Size: 507 1838 3360 1802 Fragments arranged by size: 3360 1838 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2262 2871 4954 Size: 54 1425 50 275 18 432 609 2083 Cuts at: 4954 5843 7472 8 Size: 889 1629 43 Fragments arranged by size: 2083 1629 1425 889 609 432 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4954 1537 Size: 275 3142 4090 Fragments arranged by size: 4090 3142 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2895 4146 5437 1353 Size: 1542 1251 1291 3423 Fragments arranged by size: 3423 1542 1291 1251 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6094 6468 6614 387 Size: 1064 138 4505 374 146 1280 Fragments arranged by size: 4505 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2403 2919 3582 3946 4059 5570 6167 Size: 393 771 516 663 364 113 1511 597 Cuts at: 6167 6954 7222 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1511 787 771 663 597 516 393 364 268 113 EcoO109I rG'GnC_Cy Cuts at: 2103 3066 3518 3949 4012 4013 4263 2103 Size: 963 452 431 63 1 250 5347 Fragments arranged by size: 5347 963 452 431 250 63 1 EcoRI G'AATT_C Cuts at: 4816 5194 4816 Size: 378 7129 EcoRV GAT'ATC Cuts at: 3193 3193 Size: 7507 FspI TGC'GCA Cuts at: 6013 6013 Size: 7507 HaeII r_GCGC'y Cuts at: 1678 3729 6654 1678 Size: 2051 2925 2531 Fragments arranged by size: 2925 2531 2051 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2072 2104 2761 2793 3440 Size: 32 135 32 500 32 657 32 647 Cuts at: 3440 3472 3625 3657 4047 4079 4349 4381 6148 Size: 32 153 32 390 32 270 32 1767 Cuts at: 6148 6180 6222 6254 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1767 657 647 500 390 270 153 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4851 5097 669 Size: 988 281 2913 246 3079 Fragments arranged by size: 3079 2913 988 281 246 HindIII A'AGCT_T Cuts at: 748 4782 748 Size: 4034 3473 HpaI GTT'AAC Cuts at: 5097 5097 Size: 7507 KpnI G_GTAC'C Cuts at: 2497 4832 2497 Size: 2335 5172 MfeI C'AATT_G Cuts at: 5106 5106 Size: 7507 MluI A'CGCG_T Cuts at: 7383 7383 Size: 7507 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2372 3198 3498 3821 4653 4794 6621 6805 Size: 1067 826 300 323 832 141 1827 184 Cuts at: 6805 1305 Size: 2007 Fragments arranged by size: 2007 1827 1067 832 826 323 300 184 141 MscI TGG'CCA Cuts at: 10 64 1489 1832 2264 2873 10 Size: 54 1425 343 432 609 4644 Fragments arranged by size: 4644 1425 609 432 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2207 3305 3337 4074 4580 5465 5824 Size: 648 1041 1098 32 737 506 885 359 Cuts at: 5824 5983 518 Size: 159 2042 Fragments arranged by size: 2042 1098 1041 885 737 648 506 359 159 32 MspA1I CmG'CkG Cuts at: 1606 2147 2869 3084 3200 4019 4923 5600 6748 Size: 541 722 215 116 819 904 677 1148 Cuts at: 6748 6993 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 904 819 722 677 541 245 215 116 NcoI C'CATG_G Cuts at: 513 1065 3121 4072 4687 513 Size: 552 2056 951 615 3333 Fragments arranged by size: 3333 2056 951 615 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7507 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7507 NotI GC'GGCC_GC Cuts at: 4954 4954 Size: 7507 NspI r_CATG'y Cuts at: 1132 1229 2654 4865 6410 1132 Size: 97 1425 2211 1545 2229 Fragments arranged by size: 2229 2211 1545 1425 97 PasI CC'CwG_GG Cuts at: 1348 3243 4258 1348 Size: 1895 1015 4597 Fragments arranged by size: 4597 1895 1015 PciI A'CATG_T Cuts at: 6406 6406 Size: 7507 PflMI CCAn_nnn'nTGG Cuts at: 1108 3776 4431 1108 Size: 2668 655 4184 Fragments arranged by size: 4184 2668 655 PfoI T'CCnGG_A Cuts at: 3458 3458 Size: 7507 PmeI GTTT'AAAC Cuts at: 4811 4811 Size: 7507 PmlI CAC'GTG Cuts at: 2331 2331 Size: 7507 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5576 5608 7089 7121 5576 Size: 32 1481 32 5962 Fragments arranged by size: 5962 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2103 3066 3949 4263 2103 Size: 963 883 314 5347 Fragments arranged by size: 5347 963 883 314 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7507 PsiI TTA'TAA Cuts at: 5077 5077 Size: 7507 PspOMI G'GGCC_C Cuts at: 4012 4012 Size: 7507 PspXI vC'TCGA_Gb Cuts at: 3311 4439 3311 Size: 1128 6379 PstI C_TGCA'G Cuts at: 830 2630 3572 4859 830 Size: 1800 942 1287 3478 Fragments arranged by size: 3478 1800 1287 942 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7507 PvuII CAG'CTG Cuts at: 2869 3200 2869 Size: 331 7176 RsrII CG'GwC_CG Cuts at: 2080 2092 2080 Size: 12 7495 SacII CC_GC'GG Cuts at: 2148 2148 Size: 7507 SalI G'TCGA_C Cuts at: 1936 4849 1936 Size: 2913 4594 SbfI CC_TGCA'GG Cuts at: 4859 4859 Size: 7507 ScaI AGT'ACT Cuts at: 2749 3616 5755 7467 2749 Size: 867 2139 1712 2789 Fragments arranged by size: 2789 2139 1712 867 SfcI C'TryA_G Cuts at: 826 1045 2626 3568 4757 4855 5990 6671 6862 Size: 219 1581 942 1189 98 1135 681 191 Cuts at: 6862 826 Size: 1471 Fragments arranged by size: 1581 1471 1189 1135 942 681 219 191 98 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7507 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7507 SmaI CCC'GGG Cuts at: 1872 2548 4834 1872 Size: 676 2286 4545 Fragments arranged by size: 4545 2286 676 SmlI C'TyrA_G Cuts at: 820 1017 1943 3311 4439 5611 6512 6774 820 Size: 197 926 1368 1128 1172 901 262 1553 Fragments arranged by size: 1553 1368 1172 1128 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4804 5298 493 Size: 4311 494 2702 Fragments arranged by size: 4311 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7507 SphI G_CATG'C Cuts at: 1132 2654 4865 1132 Size: 1522 2211 3774 Fragments arranged by size: 3774 2211 1522 SspI AAT'ATT Cuts at: 5 52 5431 5 Size: 47 5379 2081 Fragments arranged by size: 5379 2081 47 StuI AGG'CCT Cuts at: 1517 3625 1517 Size: 2108 5399 StyI C'CwwG_G Cuts at: 513 1065 2679 3121 3483 4072 4687 4946 513 Size: 552 1614 442 362 589 615 259 3074 Fragments arranged by size: 3074 1614 615 589 552 442 362 259 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2215 2315 2671 2743 3123 3515 4553 Size: 1255 635 100 356 72 380 392 1038 Cuts at: 4553 5534 5551 5704 5889 6334 325 Size: 981 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1038 981 635 445 392 380 356 185 153 100 72 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2747 3614 5753 Size: 274 80 33 51 1443 770 867 2139 Cuts at: 5753 7465 96 Size: 1712 138 Fragments arranged by size: 2139 1712 1443 867 770 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3647 3679 7467 7499 3647 Size: 32 3788 32 3655 Fragments arranged by size: 3788 3655 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7507 XbaI T'CTAG_A Cuts at: 4843 4843 Size: 7507 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3520 3970 4694 3520 Size: 450 724 6333 Fragments arranged by size: 6333 724 450 XhoI C'TCGA_G Cuts at: 1943 3311 4439 1943 Size: 1368 1128 5011 Fragments arranged by size: 5011 1368 1128 XmaI C'CCGG_G Cuts at: 1870 2546 4832 1870 Size: 676 2286 4545 Fragments arranged by size: 4545 2286 676 XmnI GAAnn'nnTTC Cuts at: 5636 5636 Size: 7507 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5315 276 Size: 53 83 186 936 3781 2468 Fragments arranged by size: 3781 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PsrI SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI