Restriction map for
pFN21ASDA1388
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AhdI AocI ApaI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BalI BcgI
BciVI BclI BfrI BlnI BsaBI
BsaI BseAI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eam1105I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HpaI KpnI MamI MfeI MluNI
MroI MscI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI ScaI
SfiI SfuI SmaI SnaBI SpeI
SphI SspBI SspI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AcyI 1717 GGCGCC G[GA]^CG[CT]C
AgeI 673 ACCGGT A^CCGGT
AlwNI 283 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1646 AGCGCT AGC^GCT
AvaII 984 GGACC G^G[AT]CC
AviII 778 TGCGCA TGC^GCA
BamHI 665 GGATCC G^GATCC
BbeI 1717 GGCGCC GGCGC^C
BbrPI 1171 CACGTG CAC^GTG
BbsI 792 GAAGAC GAAGAC(2/6)
BfaI 1654 CTAG C^TAG
BglII 332 AGATCT A^GATCT
BlpI 379 GCTGAGC GC^T[ACGT]AGC
BpmI 358 CTGGAG CTGGAG(16/14)
Bpu1102I 379 GCTGAGC GC^T[ACGT]AGC
BpuAI 792 GAAGAC GAAGAC(2/6)
BsaAI 1171 CACGTG [CT]AC^GT[GA]
BsaHI 1717 GGCGCC G[GA]^CG[CT]C
BsaWI 673 ACCGGT [AT]^CCGG[AT]
BsiEI 1268 CGGCCG CG[GA][CT]^CG
BspLU11I 827 ACATGT A^CATGT
BstF5I 153 CATCC GGATG(2/0)
CelII 379 GCTGAGC GC^T[ACGT]AGC
DraII 222 AGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 1268 CGGCCG [CT]^GGCC[GA]
EagI 1268 CGGCCG C^GGCCG
EclXI 1268 CGGCCG C^GGCCG
Eco47III 1646 AGCGCT AGC^GCT
Eco52I 1268 CGGCCG C^GGCCG
EcoO109I 222 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 1046 CCATGG C^C[AT][AT]GG
FokI 153 CATCC GGATG(9/13)
FspI 778 TGCGCA TGC^GCA
Hin1I 1717 GGCGCC G[GA]^CG[CT]C
HindIII 711 AAGCTT A^AGCTT
KasI 1717 GGCGCC G^GCGCC
KspI 1703 CCGCGG CCGC^GG
MaeI 1654 CTAG C^TAG
MluI 1490 ACGCGT A^CGCGT
NarI 1717 GGCGCC GG^CGCC
NcoI 1046 CCATGG C^CATGG
NspI 827 ACATGT [GA]CATG^[CT]
PaeR7I 1183 CTCGAG C^TCGAG
PflMI 282 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 673 ACCGGT A^CCGGT
PmaCI 1171 CACGTG CAC^GTG
PmlI 1171 CACGTG CAC^GTG
SacI 646 GAGCTC GAGCT^C
SacII 1703 CCGCGG CCGC^GG
SbfI 201 CCTGCAGG CCTGCA^GG
SfoI 1717 GGCGCC GGC^GCC
Sse8387I 201 CCTGCAGG CCTGCA^GG
StyI 1046 CCATGG C^C[AT][AT]GG
TaqI 1184 TCGA T^CGA
TthHB8I 1184 TCGA T^CGA
Van91I 282 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 347 CCACTGACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1183 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaI 840 CTCGGG C^[CT]CG[GA]G
1183 CTCGAG
BanI 1508 GGCACC G^G[CT][GA]CC
1717 GGCGCC
BanII 646 GAGCTC G[GA]GC[CT]^C
1105 GAGCCC
BbvCI 61 CCTCAGC CCTCAGC(-5/-2)
508 GCTGAGG
BseRI 1404 CTCCTC GAGGAG(10/8)
1102 GAGGAG
BsmAI 1186 GAGAC GTCTC(1/5)
326 GTCTC
BsoBI 840 CTCGGG C^[CT]CG[GA]G
1183 CTCGAG
BssHII 1314 GCGCGC G^CGCGC
1682 GCGCGC
BstYI 332 AGATCT [GA]^GATC[CT]
665 GGATCC
DsaI 1046 CCATGG C^C[GA][CT]GG
1703 CCGCGG
EarI 1192 CTCTTC CTCTTC(1/4)
1407 CTCTTC
HinfI 892 GAGTC G^A[ACGT]TC
1188 GACTC
Ksp632I 1192 CTCTTC CTCTTC(1/4)
1407 CTCTTC
MflI 332 AGATCT [GA]^GATC[CT]
665 GGATCC
PleI 1188 GACTC GAGTC(4/5)
892 GAGTC
XhoII 332 AGATCT [GA]^GATC[CT]
665 GGATCC
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
Alw44I AlwI ApaLI Asp700I AspHI
BbvI BcnI BglI BmyI BsaJI
BsgI BsiHKAI BsiYI BslI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraIII
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SgrAI SmlI StuI TseI
Tsp45I TspRI XmnI