(Circular) (Six-base) MAPSORT of: pFN21ASDA1388.seq Check: 132 from: 1 to: 6475 pFN21ASDA1388 6475 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3186 3186 Size: 6475 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4285 278 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3818 1937 Size: 1881 4594 Acc65I G'GTAC_C Cuts at: 3796 3796 Size: 6475 AclI AA'CG_TT Cuts at: 1563 4602 4975 1563 Size: 3039 373 3063 Fragments arranged by size: 3063 3039 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2722 4538 5922 6190 1632 Size: 1090 1816 1384 268 1917 Fragments arranged by size: 1917 1816 1384 1090 268 AfeI AGC'GCT Cuts at: 3671 3671 Size: 6475 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6278 AflIII A'CryG_T Cuts at: 1203 2850 2892 3513 5374 6351 1203 Size: 1647 42 621 1861 977 1327 Fragments arranged by size: 1861 1647 1327 977 621 42 AgeI A'CCGG_T Cuts at: 2696 5306 2696 Size: 2610 3865 AhdI GACnn_n'nnGTC Cuts at: 5204 5204 Size: 6475 AleI CACnn'nnGTG Cuts at: 1166 2697 3481 1166 Size: 1531 784 4160 Fragments arranged by size: 4160 1531 784 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2198 3732 1184 Size: 1014 1534 3927 Fragments arranged by size: 3927 1534 1014 AlwNI CAG_nnn'CTG Cuts at: 2311 4218 5790 2311 Size: 1907 1572 2996 Fragments arranged by size: 2996 1907 1572 ApaLI G'TGCA_C Cuts at: 2338 2716 2759 2941 3197 3626 4532 5688 2338 Size: 378 43 182 256 429 906 1156 3125 Fragments arranged by size: 3125 1156 906 429 378 256 182 43 ApoI r'AATT_y Cuts at: 1493 3784 4010 4162 6271 1493 Size: 2291 226 152 2109 1697 Fragments arranged by size: 2291 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5029 160 Size: 872 3997 1606 Fragments arranged by size: 3997 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 AvaI C'yCGr_G Cuts at: 1870 1943 2863 3206 3800 1870 Size: 73 920 343 594 4545 Fragments arranged by size: 4545 920 594 343 73 BamHI G'GATC_C Cuts at: 2688 3805 4172 2688 Size: 1117 367 4991 Fragments arranged by size: 4991 1117 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3531 3740 3796 5251 618 Size: 325 217 627 1744 209 56 1455 1842 Fragments arranged by size: 1842 1744 1455 627 325 217 209 56 BanII G_rGCy'C Cuts at: 1391 2673 3132 3794 1391 Size: 1282 459 662 4072 Fragments arranged by size: 4072 1282 662 459 BbeI G_GCGC'C Cuts at: 3744 3744 Size: 6475 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2822 928 Size: 971 923 4581 Fragments arranged by size: 4581 971 923 BbvCI CC'TCA_GC Cuts at: 2085 2532 2085 Size: 447 6028 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4666 4700 1903 Size: 34 2729 34 3678 Fragments arranged by size: 3678 2729 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4367 5577 4367 Size: 1210 5265 BclI T'GATC_A Cuts at: 1548 1548 Size: 6475 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3072 3240 3285 Size: 107 122 71 631 605 1400 168 45 Cuts at: 3285 3728 5086 136 Size: 443 1358 1525 Fragments arranged by size: 1525 1400 1358 631 605 443 168 122 107 71 45 BglII A'GATC_T Cuts at: 2355 6470 2355 Size: 4115 2360 BlpI GC'TnA_GC Cuts at: 2403 3892 2403 Size: 1489 4986 Bme1580I G_kGCm'C Cuts at: 1790 2141 2342 2720 2763 2945 3068 3201 3227 Size: 351 201 378 43 182 123 133 26 Cuts at: 3227 3314 3488 3572 3630 4536 5692 1790 Size: 87 174 84 58 906 1156 2573 Fragments arranged by size: 2573 1156 906 378 351 201 182 174 133 123 87 84 58 43 26 BmgBI CAC'GTC Cuts at: 1318 3682 1318 Size: 2364 4111 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4206 5164 442 Size: 416 53 83 399 24 2789 958 1753 Fragments arranged by size: 2789 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6475 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2402 5135 1239 Size: 1163 2733 2579 Fragments arranged by size: 2733 2579 1163 Bpu10I CC'TnA_GC Cuts at: 2085 2532 2085 Size: 447 6028 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2336 3464 4600 5465 5763 2336 Size: 1128 1136 865 298 3048 Fragments arranged by size: 3048 1136 1128 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5138 882 Size: 617 3639 2219 Fragments arranged by size: 3639 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3196 3772 4266 493 Size: 711 1992 576 494 2702 Fragments arranged by size: 2702 1992 711 576 494 BsaBI GATnn'nnATC Cuts at: 6469 6469 Size: 6475 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3741 4282 4664 5299 Size: 53 83 186 936 2208 541 382 635 Cuts at: 5299 275 Size: 1451 Fragments arranged by size: 2208 1451 936 635 541 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2696 4908 5306 5580 5727 6138 2696 Size: 2212 398 274 147 411 3033 Fragments arranged by size: 3033 2212 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2116 2146 2375 2405 2462 Size: 30 165 30 713 30 229 30 57 Cuts at: 2462 2492 1178 Size: 30 5161 Fragments arranged by size: 5161 713 229 165 57 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3140 3418 1186 Size: 1954 278 4243 Fragments arranged by size: 4243 1954 278 BseYI C'CCAG_C Cuts at: 3170 5678 6174 3170 Size: 2508 496 3471 Fragments arranged by size: 3471 2508 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2417 3385 3464 1163 Size: 573 681 968 79 4174 Fragments arranged by size: 4174 968 681 573 79 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3294 3925 4686 5714 1540 Size: 275 186 1293 631 761 1028 2301 Fragments arranged by size: 2301 1293 1028 761 631 275 186 BsiHKAI G_wGCw'C Cuts at: 2124 2304 2342 2388 2673 2720 2763 2874 2945 Size: 180 38 46 285 47 43 111 71 Cuts at: 2945 3201 3630 3702 4536 4621 5692 2124 Size: 256 429 72 834 85 1071 2907 Fragments arranged by size: 2907 1071 834 429 285 256 180 111 85 72 71 47 46 43 38 BsmI GAATG_Cn' Cuts at: 3986 4079 3986 Size: 93 6382 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2141 2304 2342 2388 2673 2720 Size: 399 334 17 163 38 46 285 47 Cuts at: 2720 2763 2874 2945 3068 3132 3201 3227 3314 Size: 43 111 71 123 64 69 26 87 Cuts at: 3314 3488 3572 3630 3702 3794 4536 4621 5692 Size: 174 84 58 72 92 742 85 1071 Cuts at: 5692 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 334 285 174 163 123 111 92 87 85 84 72 71 69 64 58 47 46 43 38 26 17 BspEI T'CCGG_A Cuts at: 6138 6138 Size: 6475 BspHI T'CATG_A Cuts at: 4364 6094 4364 Size: 1730 4745 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2385 3018 3186 3531 3830 844 Size: 1541 633 168 345 299 3489 Fragments arranged by size: 3489 1541 633 345 299 168 BsrBI CCG'CTC Cuts at: 1394 1823 2328 2686 2788 2832 2959 3043 3298 Size: 429 505 358 102 44 127 84 255 Cuts at: 3298 4362 1394 Size: 1064 3507 Fragments arranged by size: 3507 1064 505 429 358 255 127 102 84 44 BsrDI GCAATG_nn' Cuts at: 65 1248 4970 5144 65 Size: 1183 3722 174 1396 Fragments arranged by size: 3722 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2696 3105 3534 5119 5306 1700 Size: 110 886 409 429 1585 187 2869 Fragments arranged by size: 2869 1585 886 429 409 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6475 BssHII G'CGCG_C Cuts at: 1925 3337 3705 1925 Size: 1412 368 4695 Fragments arranged by size: 4695 1412 368 BssSI C'ACGA_G Cuts at: 4535 5547 4535 Size: 1012 5463 BstAPI GCAn_nnn'nTGC Cuts at: 794 2128 2758 2895 3408 794 Size: 1334 630 137 513 3861 Fragments arranged by size: 3861 1334 630 513 137 BstBI TT'CG_AA Cuts at: 2007 5279 2007 Size: 3272 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6475 BstYI r'GATC_y Cuts at: 1972 2355 2688 3805 4172 4557 4574 6026 6112 Size: 383 333 1117 367 385 17 1452 86 Cuts at: 6112 6124 6470 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1117 385 383 367 346 333 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3069 3726 513 Size: 552 2004 657 3262 Fragments arranged by size: 3262 2004 657 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5736 Fragments arranged by size: 5736 683 56 BtsI GCAGTG_nn' Cuts at: 3991 4785 4805 3991 Size: 794 20 5661 Fragments arranged by size: 5661 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6475 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6440 DraI TTT'AAA Cuts at: 3779 3779 Size: 6475 DraIII CAC_nnn'GTG Cuts at: 2346 2724 2758 2842 2346 Size: 378 34 84 5979 Fragments arranged by size: 5979 378 84 34 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5482 809 Size: 507 4166 1802 Fragments arranged by size: 4166 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3291 3922 4811 Size: 54 1425 50 275 18 1461 631 889 Cuts at: 4811 6440 8 Size: 1629 43 Fragments arranged by size: 1629 1461 1425 889 631 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3291 3922 1537 Size: 275 1479 631 4090 Fragments arranged by size: 4090 1479 631 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3221 3436 4405 1353 Size: 1868 215 969 3423 Fragments arranged by size: 3423 1868 969 215 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2159 5062 5436 5582 387 Size: 1064 138 570 2903 374 146 1280 Fragments arranged by size: 2903 1280 1064 570 374 146 138 EcoICRI GAG'CTC Cuts at: 2671 2671 Size: 6475 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2402 2722 4538 5135 5922 6190 1239 Size: 393 770 320 1816 597 787 268 1524 Fragments arranged by size: 1816 1524 787 770 597 393 320 268 EcoO109I rG'GnC_Cy Cuts at: 2246 2246 Size: 6475 EcoRI G'AATT_C Cuts at: 3784 4162 3784 Size: 378 6097 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2458 2458 Size: 6475 FspI TGC'GCA Cuts at: 2803 4981 2803 Size: 2178 4297 HaeII r_GCGC'y Cuts at: 1678 3039 3673 3744 5622 1678 Size: 1361 634 71 1878 2531 Fragments arranged by size: 2531 1878 1361 634 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5116 5148 5190 5222 1373 Size: 32 135 32 3544 32 42 32 2626 Fragments arranged by size: 3544 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3819 4065 669 Size: 988 281 1881 246 3079 Fragments arranged by size: 3079 1881 988 281 246 HindIII A'AGCT_T Cuts at: 748 2734 748 Size: 1986 4489 HpaI GTT'AAC Cuts at: 4065 4065 Size: 6475 KasI G'GCGC_C Cuts at: 3740 3740 Size: 6475 KpnI G_GTAC'C Cuts at: 3800 3800 Size: 6475 MfeI C'AATT_G Cuts at: 4074 4074 Size: 6475 MluI A'CGCG_T Cuts at: 3513 6351 3513 Size: 2838 3637 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2007 2646 2901 5589 5773 1305 Size: 702 639 255 2688 184 2007 Fragments arranged by size: 2688 2007 702 639 255 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4653 Fragments arranged by size: 4653 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2335 2697 3481 4433 4792 4951 518 Size: 648 1169 362 784 952 359 159 2042 Fragments arranged by size: 2042 1169 952 784 648 362 359 159 MspA1I CmG'CkG Cuts at: 1606 2531 2540 2631 2886 2970 3364 3453 3529 Size: 925 9 91 255 84 394 89 76 Cuts at: 3529 3598 3728 3734 3891 4568 5716 5961 1606 Size: 69 130 6 157 677 1148 245 2120 Fragments arranged by size: 2120 1148 925 677 394 255 245 157 130 91 89 84 76 69 9 6 NarI GG'CG_CC Cuts at: 3741 3741 Size: 6475 NcoI C'CATG_G Cuts at: 513 1065 3069 513 Size: 552 2004 3919 Fragments arranged by size: 3919 2004 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6475 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6475 NotI GC'GGCC_GC Cuts at: 3922 3922 Size: 6475 NspI r_CATG'y Cuts at: 1132 1229 2854 3833 5378 1132 Size: 97 1625 979 1545 2229 Fragments arranged by size: 2229 1625 1545 979 97 PasI CC'CwG_GG Cuts at: 1348 2155 1348 Size: 807 5668 PciI A'CATG_T Cuts at: 2850 5374 2850 Size: 2524 3951 PflMI CCAn_nnn'nTGG Cuts at: 1108 2311 1108 Size: 1203 5272 PmeI GTTT'AAAC Cuts at: 3779 3779 Size: 6475 PmlI CAC'GTG Cuts at: 3196 3196 Size: 6475 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3629 3661 4544 4576 6057 6089 3629 Size: 32 883 32 1481 32 4015 Fragments arranged by size: 4015 1481 883 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6475 PsiI TTA'TAA Cuts at: 4045 4045 Size: 6475 PstI C_TGCA'G Cuts at: 830 2187 2205 2229 2934 3354 3528 3827 830 Size: 1357 18 24 705 420 174 299 3478 Fragments arranged by size: 3478 1357 705 420 299 174 24 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6475 PvuII CAG'CTG Cuts at: 2531 2631 3364 2531 Size: 100 733 5642 Fragments arranged by size: 5642 733 100 SacI G_AGCT'C Cuts at: 2673 2673 Size: 6475 SacII CC_GC'GG Cuts at: 3729 3729 Size: 6475 SalI G'TCGA_C Cuts at: 1936 3817 1936 Size: 1881 4594 SbfI CC_TGCA'GG Cuts at: 2229 3827 2229 Size: 1598 4877 ScaI AGT'ACT Cuts at: 4723 6435 4723 Size: 1712 4763 SexAI A'CCwGG_T Cuts at: 2754 2838 3747 2754 Size: 84 909 5482 Fragments arranged by size: 5482 909 84 SfcI C'TryA_G Cuts at: 826 1045 2183 2201 2225 2626 2930 3350 3524 Size: 219 1138 18 24 401 304 420 174 Cuts at: 3524 3823 4958 5639 5830 826 Size: 299 1135 681 191 1471 Fragments arranged by size: 1471 1138 1135 681 420 401 304 299 219 191 174 24 18 SfoI GGC'GCC Cuts at: 3742 3742 Size: 6475 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6475 SgrAI Cr'CCGG_yG Cuts at: 1700 2696 3105 3534 1700 Size: 996 409 429 4641 Fragments arranged by size: 4641 996 429 409 SmaI CCC'GGG Cuts at: 1872 3802 1872 Size: 1930 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2351 3206 3479 4579 5480 5742 Size: 197 926 408 855 273 1100 901 262 Cuts at: 5742 820 Size: 1553 Fragments arranged by size: 1553 1100 926 901 855 408 273 262 197 SnaBI TAC'GTA Cuts at: 493 3772 4266 493 Size: 3279 494 2702 Fragments arranged by size: 3279 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6475 SphI G_CATG'C Cuts at: 1132 3833 1132 Size: 2701 3774 SspI AAT'ATT Cuts at: 5 52 4399 5 Size: 47 4347 2081 Fragments arranged by size: 4347 2081 47 StuI AGG'CCT Cuts at: 1517 2990 3329 3587 1517 Size: 1473 339 258 4405 Fragments arranged by size: 4405 1473 339 258 StyI C'CwwG_G Cuts at: 513 1065 3069 3914 513 Size: 552 2004 845 3074 Fragments arranged by size: 3074 2004 845 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3137 4502 4519 4672 4857 5302 325 Size: 1255 1557 1365 17 153 185 445 1498 Fragments arranged by size: 1557 1498 1365 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4721 6433 96 Size: 274 80 33 51 1443 2744 1712 138 Fragments arranged by size: 2744 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2116 2148 2375 2407 2462 2494 6435 6467 2116 Size: 32 227 32 55 32 3941 32 2124 Fragments arranged by size: 3941 2124 227 55 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6475 XbaI T'CTAG_A Cuts at: 3811 3811 Size: 6475 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2377 2377 Size: 6475 XhoI C'TCGA_G Cuts at: 1943 3206 1943 Size: 1263 5212 XmaI C'CCGG_G Cuts at: 1870 3800 1870 Size: 1930 4545 XmnI GAAnn'nnTTC Cuts at: 2414 2819 2990 3503 4604 2414 Size: 405 171 513 1101 4285 Fragments arranged by size: 4285 1101 513 405 171 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4283 276 Size: 53 83 186 936 2749 2468 Fragments arranged by size: 2749 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI