Restriction map for
pFN21ASDA1388
(Linear) (Six-base) MAPSORT of: KIAA1388.seq Check: 4448 from: 1 to: 1746
KIAA1388.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1163 1746
Size: 1163 583
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 699 1746
Size: 699 1047
AfeI AGC'GCT
Cuts at: 0 1648 1746
Size: 1648 98
AflIII A'CryG_T
Cuts at: 0 827 869 1490 1746
Size: 827 42 621 256
Fragments arranged by size:
827 621 256 42
AgeI A'CCGG_T
Cuts at: 0 673 1746
Size: 673 1073
AleI CACnn'nnGTG
Cuts at: 0 674 1458 1746
Size: 674 784 288
Fragments arranged by size:
784 674 288
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 175 1709 1746
Size: 175 1534 37
Fragments arranged by size:
1534 175 37
AlwNI CAG_nnn'CTG
Cuts at: 0 288 1746
Size: 288 1458
ApaLI G'TGCA_C
Cuts at: 0 315 693 736 918 1174 1603 1746
Size: 315 378 43 182 256 429 143
Fragments arranged by size:
429 378 315 256 182 143 43
AvaI C'yCGr_G
Cuts at: 0 840 1183 1746
Size: 840 343 563
Fragments arranged by size:
840 563 343
BamHI G'GATC_C
Cuts at: 0 665 1746
Size: 665 1081
BanI G'GyrC_C
Cuts at: 0 1508 1717 1746
Size: 1508 209 29
Fragments arranged by size:
1508 209 29
BanII G_rGCy'C
Cuts at: 0 650 1109 1746
Size: 650 459 637
Fragments arranged by size:
650 637 459
BbeI G_GCGC'C
Cuts at: 0 1721 1746
Size: 1721 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 799 1746
Size: 799 947
BbvCI CC'TCA_GC
Cuts at: 0 62 509 1746
Size: 62 447 1237
Fragments arranged by size:
1237 447 62
BglI GCCn_nnn'nGGC
Cuts at: 0 1049 1217 1262 1705 1746
Size: 1049 168 45 443 41
Fragments arranged by size:
1049 443 168 45 41
BglII A'GATC_T
Cuts at: 0 332 1746
Size: 332 1414
BlpI GC'TnA_GC
Cuts at: 0 380 1746
Size: 380 1366
Bme1580I G_kGCm'C
Cuts at: 0 118 319 697 740 922 1045 1178 1204
Size: 118 201 378 43 182 123 133 26
Cuts at: 1204 1291 1465 1549 1607 1746
Size: 87 174 84 58 139
Fragments arranged by size:
378 201 182 174 139 133 123 118
87 84 58 43 26
BmgBI CAC'GTC
Cuts at: 0 1659 1746
Size: 1659 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 1746
Size: 379 1367
Bpu10I CC'TnA_GC
Cuts at: 0 62 509 1746
Size: 62 447 1237
Fragments arranged by size:
1237 447 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 1441 1746
Size: 313 1128 305
Fragments arranged by size:
1128 313 305
BsaAI yAC'GTr
Cuts at: 0 1173 1746
Size: 1173 573
BsaHI Gr'CG_yC
Cuts at: 0 1718 1746
Size: 1718 28
BsaWI w'CCGG_w
Cuts at: 0 673 1746
Size: 673 1073
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 93 123 352 382 439 469 1746
Size: 93 30 229 30 57 30 1277
Fragments arranged by size:
1277 229 93 57 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1117 1395 1746
Size: 1117 278 351
Fragments arranged by size:
1117 351 278
BseYI C'CCAG_C
Cuts at: 0 1147 1746
Size: 1147 599
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1362 1441 1746
Size: 394 968 79 305
Fragments arranged by size:
968 394 305 79
BsiEI CG_ry'CG
Cuts at: 0 1271 1746
Size: 1271 475
BsiHKAI G_wGCw'C
Cuts at: 0 101 281 319 365 650 697 740 851
Size: 101 180 38 46 285 47 43 111
Cuts at: 851 922 1178 1607 1679 1746
Size: 71 256 429 72 67
Fragments arranged by size:
429 285 256 180 111 101 72 71
67 47 46 43 38
Bsp1286I G_dGCh'C
Cuts at: 0 101 118 281 319 365 650 697 740
Size: 101 17 163 38 46 285 47 43
Cuts at: 740 851 922 1045 1109 1178 1204 1291 1465
Size: 111 71 123 64 69 26 87 174
Cuts at: 1465 1549 1607 1679 1746
Size: 84 58 72 67
Fragments arranged by size:
285 174 163 123 111 101 87 84
72 71 69 67 64 58 47 46
43 38 26 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 362 995 1163 1508 1746
Size: 362 633 168 345 238
Fragments arranged by size:
633 362 345 238 168
BsrBI CCG'CTC
Cuts at: 0 305 663 765 809 936 1020 1275 1746
Size: 305 358 102 44 127 84 255 471
Fragments arranged by size:
471 358 305 255 127 102 84 44
BsrFI r'CCGG_y
Cuts at: 0 673 1082 1511 1746
Size: 673 409 429 235
Fragments arranged by size:
673 429 409 235
BssHII G'CGCG_C
Cuts at: 0 1314 1682 1746
Size: 1314 368 64
Fragments arranged by size:
1314 368 64
BstAPI GCAn_nnn'nTGC
Cuts at: 0 105 735 872 1385 1746
Size: 105 630 137 513 361
Fragments arranged by size:
630 513 361 137 105
BstYI r'GATC_y
Cuts at: 0 332 665 1746
Size: 332 333 1081
Fragments arranged by size:
1081 333 332
BtgI C'CryG_G
Cuts at: 0 1046 1703 1746
Size: 1046 657 43
Fragments arranged by size:
1046 657 43
DraIII CAC_nnn'GTG
Cuts at: 0 323 701 735 819 1746
Size: 323 378 34 84 927
Fragments arranged by size:
927 378 323 84 34
EaeI y'GGCC_r
Cuts at: 0 1268 1746
Size: 1268 478
EagI C'GGCC_G
Cuts at: 0 1268 1746
Size: 1268 478
EarI CTCTTCn'nnn_
Cuts at: 0 1198 1413 1746
Size: 1198 215 333
Fragments arranged by size:
1198 333 215
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 136 1746
Size: 136 1610
EcoICRI GAG'CTC
Cuts at: 0 648 1746
Size: 648 1098
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 699 1746
Size: 379 320 1047
Fragments arranged by size:
1047 379 320
EcoO109I rG'GnC_Cy
Cuts at: 0 223 1746
Size: 223 1523
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 435 1746
Size: 435 1311
FspI TGC'GCA
Cuts at: 0 780 1746
Size: 780 966
HaeII r_GCGC'y
Cuts at: 0 1016 1650 1721 1746
Size: 1016 634 71 25
Fragments arranged by size:
1016 634 71 25
HindIII A'AGCT_T
Cuts at: 0 711 1746
Size: 711 1035
KasI G'GCGC_C
Cuts at: 0 1717 1746
Size: 1717 29
MluI A'CGCG_T
Cuts at: 0 1490 1746
Size: 1490 256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 878 1746
Size: 623 255 868
Fragments arranged by size:
868 623 255
MslI CAynn'nnrTG
Cuts at: 0 312 674 1458 1746
Size: 312 362 784 288
Fragments arranged by size:
784 362 312 288
MspA1I CmG'CkG
Cuts at: 0 508 517 608 863 947 1341 1430 1506
Size: 508 9 91 255 84 394 89 76
Cuts at: 1506 1575 1705 1711 1746
Size: 69 130 6 35
Fragments arranged by size:
508 394 255 130 91 89 84 76
69 35 9 6
NarI GG'CG_CC
Cuts at: 0 1718 1746
Size: 1718 28
NcoI C'CATG_G
Cuts at: 0 1046 1746
Size: 1046 700
NspI r_CATG'y
Cuts at: 0 831 1746
Size: 831 915
PasI CC'CwG_GG
Cuts at: 0 132 1746
Size: 132 1614
PciI A'CATG_T
Cuts at: 0 827 1746
Size: 827 919
PflMI CCAn_nnn'nTGG
Cuts at: 0 288 1746
Size: 288 1458
PmlI CAC'GTG
Cuts at: 0 1173 1746
Size: 1173 573
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1606 1638 1746
Size: 1606 32 108
Fragments arranged by size:
1606 108 32
PstI C_TGCA'G
Cuts at: 0 164 182 206 911 1331 1505 1746
Size: 164 18 24 705 420 174 241
Fragments arranged by size:
705 420 241 174 164 24 18
PvuII CAG'CTG
Cuts at: 0 508 608 1341 1746
Size: 508 100 733 405
Fragments arranged by size:
733 508 405 100
SacI G_AGCT'C
Cuts at: 0 650 1746
Size: 650 1096
SacII CC_GC'GG
Cuts at: 0 1706 1746
Size: 1706 40
SbfI CC_TGCA'GG
Cuts at: 0 206 1746
Size: 206 1540
SexAI A'CCwGG_T
Cuts at: 0 731 815 1724 1746
Size: 731 84 909 22
Fragments arranged by size:
909 731 84 22
SfcI C'TryA_G
Cuts at: 0 160 178 202 603 907 1327 1501 1746
Size: 160 18 24 401 304 420 174 245
Fragments arranged by size:
420 401 304 245 174 160 24 18
SfoI GGC'GCC
Cuts at: 0 1719 1746
Size: 1719 27
SgrAI Cr'CCGG_yG
Cuts at: 0 673 1082 1511 1746
Size: 673 409 429 235
Fragments arranged by size:
673 429 409 235
SmlI C'TyrA_G
Cuts at: 0 328 1183 1456 1746
Size: 328 855 273 290
Fragments arranged by size:
855 328 290 273
StuI AGG'CCT
Cuts at: 0 967 1306 1564 1746
Size: 967 339 258 182
Fragments arranged by size:
967 339 258 182
StyI C'CwwG_G
Cuts at: 0 1046 1746
Size: 1046 700
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1114 1746
Size: 1114 632
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 93 125 352 384 439 471 1746
Size: 93 32 227 32 55 32 1275
Fragments arranged by size:
1275 227 93 55 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 354 1746
Size: 354 1392
XhoI C'TCGA_G
Cuts at: 0 1183 1746
Size: 1183 563
XmnI GAAnn'nnTTC
Cuts at: 0 391 796 967 1480 1746
Size: 391 405 171 513 266
Fragments arranged by size:
513 405 391 266 171
Enzymes that do cut:
AarI AcuI AfeI AflIII AgeI AleI AlfI AlwNI
ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BglI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstYI
BtgI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I FalI FspI HaeII HindIII KasI MluI MmeI
MslI MspA1I NarI NcoI NspI PasI PciI PflMI
PmlI PpiI PstI PvuII SacI SacII SbfI SexAI
SfcI SfoI SgrAI SmlI StuI StyI TaqII TstI
XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AhdI AloI ApaI
ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI
BclI BfrBI BmrI BmtI BplI BsaI BsaBI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DrdI EcoNI EcoRI EcoRV FseI FspAI Hin4I
HincII HpaI KpnI MfeI MscI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PfoI PmeI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI
SanDI SapI ScaI SfiI SgfI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XmaI
ZraI