(Circular) (Six-base) MAPSORT of: pFN21ASDA1383.seq Check: 6179 from: 1 to: 7444 pFN21ASDA1383 7444 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5254 278 Size: 53 83 186 936 3718 2468 Fragments arranged by size: 3718 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4787 1937 Size: 2850 4594 Acc65I G'GTAC_C Cuts at: 4765 4765 Size: 7444 AclI AA'CG_TT Cuts at: 1563 5571 5944 1563 Size: 4008 373 3063 Fragments arranged by size: 4008 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3378 3395 3717 4136 4173 5507 6891 7159 Size: 1746 17 322 419 37 1334 1384 268 Cuts at: 7159 1632 Size: 1917 Fragments arranged by size: 1917 1746 1384 1334 419 322 268 37 17 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7247 AflIII A'CryG_T Cuts at: 1203 2837 3164 6343 7320 1203 Size: 1634 327 3179 977 1327 Fragments arranged by size: 3179 1634 1327 977 327 AgeI A'CCGG_T Cuts at: 6275 6275 Size: 7444 AhdI GACnn_n'nnGTC Cuts at: 6173 6173 Size: 7444 AleI CACnn'nnGTG Cuts at: 1166 4265 1166 Size: 3099 4345 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7444 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4596 4628 4596 Size: 32 7412 AlwNI CAG_nnn'CTG Cuts at: 2416 2674 2849 5187 6759 2416 Size: 258 175 2338 1572 3101 Fragments arranged by size: 3101 2338 1572 258 175 ApaLI G'TGCA_C Cuts at: 5501 6657 5501 Size: 1156 6288 ApoI r'AATT_y Cuts at: 1493 3626 3633 3889 4352 4753 4979 5131 7240 Size: 2133 7 256 463 401 226 152 2109 Cuts at: 7240 1493 Size: 1697 Fragments arranged by size: 2133 2109 1697 463 401 256 226 152 7 AseI AT'TA_AT Cuts at: 160 1032 3189 5998 160 Size: 872 2157 2809 1606 Fragments arranged by size: 2809 2157 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7444 AvaI C'yCGr_G Cuts at: 1870 1943 4769 1870 Size: 73 2826 4545 Fragments arranged by size: 4545 2826 73 BamHI G'GATC_C Cuts at: 4774 5141 4774 Size: 367 7077 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2391 3473 4765 6220 618 Size: 325 217 627 604 1082 1292 1455 1842 Fragments arranged by size: 1842 1455 1292 1082 627 604 325 217 BanII G_rGCy'C Cuts at: 1391 3780 4501 4763 1391 Size: 2389 721 262 4072 Fragments arranged by size: 4072 2389 721 262 BbeI G_GCGC'C Cuts at: 2395 2395 Size: 7444 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3401 928 Size: 971 1502 4971 Fragments arranged by size: 4971 1502 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3317 3351 5635 5669 1903 Size: 34 1380 34 2284 34 3678 Fragments arranged by size: 3678 2284 1380 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4721 5336 6546 4721 Size: 615 1210 5619 Fragments arranged by size: 5619 1210 615 BclI T'GATC_A Cuts at: 1548 1548 Size: 7444 BfrBI ATG'CAT Cuts at: 3722 3722 Size: 7444 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2389 6055 136 Size: 107 122 71 631 605 717 3666 1525 Fragments arranged by size: 3666 1525 717 631 605 122 107 71 BglII A'GATC_T Cuts at: 7439 7439 Size: 7444 BlpI GC'TnA_GC Cuts at: 2588 4861 2588 Size: 2273 5171 Bme1580I G_kGCm'C Cuts at: 1790 2186 3478 5505 6661 1790 Size: 396 1292 2027 1156 2573 Fragments arranged by size: 2573 2027 1292 1156 396 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7444 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2083 2547 5175 Size: 416 53 83 399 24 666 464 2628 Cuts at: 5175 6133 442 Size: 958 1753 Fragments arranged by size: 2628 1753 958 666 464 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7444 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 6104 1239 Size: 839 4026 2579 Fragments arranged by size: 4026 2579 839 Bpu10I CC'TnA_GC Cuts at: 4307 4307 Size: 7444 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2618 3733 4485 5569 6434 6732 2618 Size: 1115 752 1084 865 298 3330 Fragments arranged by size: 3330 1115 1084 865 752 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2900 6107 882 Size: 617 1401 3207 2219 Fragments arranged by size: 3207 2219 1401 617 BsaAI yAC'GTr Cuts at: 493 1204 4741 5235 493 Size: 711 3537 494 2702 Fragments arranged by size: 3537 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7438 7438 Size: 7444 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2392 2399 5251 5633 Size: 53 83 186 936 859 7 2852 382 Cuts at: 5633 6268 275 Size: 635 1451 Fragments arranged by size: 2852 1451 936 859 635 382 186 83 53 7 BsaWI w'CCGG_w Cuts at: 2477 5877 6275 6549 6696 7107 2477 Size: 3400 398 274 147 411 2814 Fragments arranged by size: 3400 2814 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2150 2180 2625 2655 3924 Size: 30 165 30 747 30 445 30 1269 Cuts at: 3924 3954 1178 Size: 30 4668 Fragments arranged by size: 4668 1269 747 445 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2144 2153 2643 2646 2916 1186 Size: 958 9 490 3 270 5714 Fragments arranged by size: 5714 958 490 270 9 3 BseYI C'CCAG_C Cuts at: 2655 2722 3058 3779 6647 7143 2655 Size: 67 336 721 2868 496 2956 Fragments arranged by size: 2956 2868 721 496 336 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2318 2955 1163 Size: 573 582 637 5652 Fragments arranged by size: 5652 637 582 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2293 2472 4894 5655 6683 1540 Size: 275 186 292 179 2422 761 1028 2301 Fragments arranged by size: 2422 2301 1028 761 292 275 186 179 BsiHKAI G_wGCw'C Cuts at: 4501 5505 5590 6661 4501 Size: 1004 85 1071 5284 Fragments arranged by size: 5284 1071 1004 85 BsmI GAATG_Cn' Cuts at: 3010 4955 5048 3010 Size: 1945 93 5406 Fragments arranged by size: 5406 1945 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2186 3478 3780 4501 4763 5505 5590 Size: 399 396 1292 302 721 262 742 85 Cuts at: 5590 6661 1391 Size: 1071 2174 Fragments arranged by size: 2174 1292 1071 742 721 399 396 302 262 85 BspEI T'CCGG_A Cuts at: 2477 7107 2477 Size: 4630 2814 BspHI T'CATG_A Cuts at: 4198 5333 7063 4198 Size: 1135 1730 4579 Fragments arranged by size: 4579 1730 1135 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4593 4799 844 Size: 3749 206 3489 Fragments arranged by size: 3749 3489 206 BsrBI CCG'CTC Cuts at: 1394 1823 2044 2533 2602 2636 5331 1394 Size: 429 221 489 69 34 2695 3507 Fragments arranged by size: 3507 2695 489 429 221 69 34 BsrDI GCAATG_nn' Cuts at: 65 1248 2544 2860 4657 4691 5939 6113 65 Size: 1183 1296 316 1797 34 1248 174 1396 Fragments arranged by size: 1797 1396 1296 1248 1183 316 174 34 BsrFI r'CCGG_y Cuts at: 1700 1810 2340 2467 3351 6088 6275 1700 Size: 110 530 127 884 2737 187 2869 Fragments arranged by size: 2869 2737 884 530 187 127 110 BsrGI T'GTAC_A Cuts at: 96 4336 96 Size: 4240 3204 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7444 BssSI C'ACGA_G Cuts at: 5504 6516 5504 Size: 1012 6432 BstAPI GCAn_nnn'nTGC Cuts at: 794 2304 794 Size: 1510 5934 BstBI TT'CG_AA Cuts at: 2007 6248 2007 Size: 4241 3203 BstEII G'GTnAC_C Cuts at: 1184 4329 1184 Size: 3145 4299 BstXI CCAn_nnnn'nTGG Cuts at: 2224 2224 Size: 7444 BstYI r'GATC_y Cuts at: 1972 4774 5141 5526 5543 6995 7081 7093 7439 Size: 2802 367 385 17 1452 86 12 346 Cuts at: 7439 1972 Size: 1977 Fragments arranged by size: 2802 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3012 3012 Size: 7444 BtgI C'CryG_G Cuts at: 513 1065 2022 2190 2267 513 Size: 552 957 168 77 5690 Fragments arranged by size: 5690 957 552 168 77 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6705 Fragments arranged by size: 6705 683 56 BtsI GCAGTG_nn' Cuts at: 2124 2335 3475 4960 5754 5774 2124 Size: 211 1140 1485 794 20 3794 Fragments arranged by size: 3794 1485 1140 794 211 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7444 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7409 DraI TTT'AAA Cuts at: 3543 4748 3543 Size: 1205 6239 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6451 809 Size: 507 5135 1802 Fragments arranged by size: 5135 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2439 2469 4891 Size: 54 1425 50 275 18 609 30 2422 Cuts at: 4891 5780 7409 8 Size: 889 1629 43 Fragments arranged by size: 2422 1629 1425 889 609 275 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2469 4891 1537 Size: 275 657 2422 4090 Fragments arranged by size: 4090 2422 657 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 2130 2315 5374 1353 Size: 701 76 185 3059 3423 Fragments arranged by size: 3423 3059 701 185 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2189 2303 6031 6405 6551 387 Size: 1064 138 600 114 3728 374 146 1280 Fragments arranged by size: 3728 1280 1064 600 374 146 138 114 EcoICRI GAG'CTC Cuts at: 4499 4499 Size: 7444 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 3378 3395 3717 4136 4173 5507 Size: 393 446 1300 17 322 419 37 1334 Cuts at: 5507 6104 6891 7159 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1334 1300 787 597 446 419 393 322 268 37 17 EcoNI CCTnn'n_nnAGG Cuts at: 2879 2879 Size: 7444 EcoO109I rG'GnC_Cy Cuts at: 2421 2421 Size: 7444 EcoRI G'AATT_C Cuts at: 3633 4753 5131 3633 Size: 1120 378 5946 Fragments arranged by size: 5946 1120 378 FseI GG_CCGG'CC Cuts at: 2471 2471 Size: 7444 FspI TGC'GCA Cuts at: 5950 5950 Size: 7444 HaeII r_GCGC'y Cuts at: 1678 2253 2395 6591 1678 Size: 575 142 4196 2531 Fragments arranged by size: 4196 2531 575 142 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2150 2182 2625 2657 3305 Size: 32 135 32 578 32 443 32 648 Cuts at: 3305 3337 4118 4150 4207 4239 4379 4411 6085 Size: 32 781 32 57 32 140 32 1674 Cuts at: 6085 6117 6159 6191 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1674 781 648 578 443 140 135 57 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3991 4788 5034 669 Size: 988 281 2053 797 246 3079 Fragments arranged by size: 3079 2053 988 797 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7444 HpaI GTT'AAC Cuts at: 5034 5034 Size: 7444 KasI G'GCGC_C Cuts at: 2391 2391 Size: 7444 KpnI G_GTAC'C Cuts at: 4769 4769 Size: 7444 MfeI C'AATT_G Cuts at: 5043 5043 Size: 7444 MluI A'CGCG_T Cuts at: 7320 7320 Size: 7444 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3997 4511 6558 6742 1305 Size: 2692 514 2047 184 2007 Fragments arranged by size: 2692 2047 2007 514 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2441 10 Size: 54 1425 343 609 5013 Fragments arranged by size: 5013 1425 609 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4052 4265 5402 5761 5920 518 Size: 648 2886 213 1137 359 159 2042 Fragments arranged by size: 2886 2042 1137 648 359 213 159 MspA1I CmG'CkG Cuts at: 1606 2111 2380 2674 2722 4860 5537 6685 6930 Size: 505 269 294 48 2138 677 1148 245 Cuts at: 6930 1606 Size: 2120 Fragments arranged by size: 2138 2120 1148 677 505 294 269 245 48 NaeI GCC'GGC Cuts at: 2342 2469 2342 Size: 127 7317 NarI GG'CG_CC Cuts at: 2392 2392 Size: 7444 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5935 Fragments arranged by size: 5935 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7444 NgoMIV G'CCGG_C Cuts at: 2340 2467 2340 Size: 127 7317 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7444 NotI GC'GGCC_GC Cuts at: 4891 4891 Size: 7444 NsiI A_TGCA'T Cuts at: 3724 3724 Size: 7444 NspI r_CATG'y Cuts at: 1132 1229 2841 3168 3351 4802 6347 1132 Size: 97 1612 327 183 1451 1545 2229 Fragments arranged by size: 2229 1612 1545 1451 327 183 97 PasI CC'CwG_GG Cuts at: 1348 2387 1348 Size: 1039 6405 PciI A'CATG_T Cuts at: 2837 3164 6343 2837 Size: 327 3179 3938 Fragments arranged by size: 3938 3179 327 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7444 PfoI T'CCnGG_A Cuts at: 4614 4723 4614 Size: 109 7335 PmeI GTTT'AAAC Cuts at: 4748 4748 Size: 7444 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5513 5545 7026 7058 5513 Size: 32 1481 32 5899 Fragments arranged by size: 5899 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7444 PsiI TTA'TAA Cuts at: 5014 5014 Size: 7444 PstI C_TGCA'G Cuts at: 830 2322 2379 2679 3097 3670 4796 830 Size: 1492 57 300 418 573 1126 3478 Fragments arranged by size: 3478 1492 1126 573 418 300 57 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7444 PvuII CAG'CTG Cuts at: 2380 2674 2380 Size: 294 7150 SacI G_AGCT'C Cuts at: 4501 4501 Size: 7444 SalI G'TCGA_C Cuts at: 1936 4786 1936 Size: 2850 4594 SapI GCTCTTCn'nnn_ Cuts at: 2054 2054 Size: 7444 SbfI CC_TGCA'GG Cuts at: 4796 4796 Size: 7444 ScaI AGT'ACT Cuts at: 5692 7404 5692 Size: 1712 5732 SfcI C'TryA_G Cuts at: 826 1045 2318 2375 2675 3093 3666 4792 5927 Size: 219 1273 57 300 418 573 1126 1135 Cuts at: 5927 6608 6799 826 Size: 681 191 1471 Fragments arranged by size: 1471 1273 1135 1126 681 573 418 300 219 191 57 SfoI GGC'GCC Cuts at: 2393 2393 Size: 7444 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7444 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7444 SmaI CCC'GGG Cuts at: 1872 4771 1872 Size: 2899 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2597 3748 4500 5548 6449 6711 Size: 197 926 654 1151 752 1048 901 262 Cuts at: 6711 820 Size: 1553 Fragments arranged by size: 1553 1151 1048 926 901 752 654 262 197 SnaBI TAC'GTA Cuts at: 493 4741 5235 493 Size: 4248 494 2702 Fragments arranged by size: 4248 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7444 SphI G_CATG'C Cuts at: 1132 4802 1132 Size: 3670 3774 SspI AAT'ATT Cuts at: 5 52 3455 3925 4675 5368 5 Size: 47 3403 470 750 693 2081 Fragments arranged by size: 3403 2081 750 693 470 47 StuI AGG'CCT Cuts at: 1517 2162 1517 Size: 645 6799 StyI C'CwwG_G Cuts at: 513 1065 2022 4883 513 Size: 552 957 2861 3074 Fragments arranged by size: 3074 2861 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2065 2279 5471 5488 5641 5826 6271 Size: 1255 485 214 3192 17 153 185 445 Cuts at: 6271 325 Size: 1498 Fragments arranged by size: 3192 1498 1255 485 445 214 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4336 4625 5690 Size: 274 80 33 51 1443 2359 289 1065 Cuts at: 5690 7402 96 Size: 1712 138 Fragments arranged by size: 2359 1712 1443 1065 289 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3924 3956 7404 7436 3924 Size: 32 3448 32 3932 Fragments arranged by size: 3932 3448 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7444 XbaI T'CTAG_A Cuts at: 4780 4780 Size: 7444 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7444 XmaI C'CCGG_G Cuts at: 1870 4769 1870 Size: 2899 4545 XmnI GAAnn'nnTTC Cuts at: 5573 5573 Size: 7444 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5252 276 Size: 53 83 186 936 3718 2468 Fragments arranged by size: 3718 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FspAI NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI