(Circular) (Six-base) MAPSORT of: pFN21ASDA1382.seq Check: 2456 from: 1 to: 6247 pFN21ASDA1382 6247 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4057 278 Size: 53 83 186 936 2521 2468 Fragments arranged by size: 2521 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3590 1937 Size: 1653 4594 Acc65I G'GTAC_C Cuts at: 3568 3568 Size: 6247 AclI AA'CG_TT Cuts at: 1563 4374 4747 1563 Size: 2811 373 3063 Fragments arranged by size: 3063 2811 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4310 5694 5962 1632 Size: 2678 1384 268 1917 Fragments arranged by size: 2678 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6050 AflIII A'CryG_T Cuts at: 1203 3079 5146 6123 1203 Size: 1876 2067 977 1327 Fragments arranged by size: 2067 1876 1327 977 AgeI A'CCGG_T Cuts at: 5078 5078 Size: 6247 AhdI GACnn_n'nnGTC Cuts at: 4976 4976 Size: 6247 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6247 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6247 AlwNI CAG_nnn'CTG Cuts at: 2474 3990 5562 2474 Size: 1516 1572 3159 Fragments arranged by size: 3159 1572 1516 ApaLI G'TGCA_C Cuts at: 4304 5460 4304 Size: 1156 5091 ApoI r'AATT_y Cuts at: 1493 2188 2216 2662 2741 3556 3782 3934 6043 Size: 695 28 446 79 815 226 152 2109 Cuts at: 6043 1493 Size: 1697 Fragments arranged by size: 2109 1697 815 695 446 226 152 79 28 AseI AT'TA_AT Cuts at: 160 1032 4801 160 Size: 872 3769 1606 Fragments arranged by size: 3769 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 AvaI C'yCGr_G Cuts at: 1870 1943 3572 1870 Size: 73 1629 4545 Fragments arranged by size: 4545 1629 73 BamHI G'GATC_C Cuts at: 3577 3944 3577 Size: 367 5880 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3568 5023 618 Size: 325 217 627 1781 1455 1842 Fragments arranged by size: 1842 1781 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3566 1391 Size: 2175 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2073 2399 928 Size: 971 174 326 4776 Fragments arranged by size: 4776 971 326 174 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4438 4472 1903 Size: 34 2501 34 3678 Fragments arranged by size: 3678 2501 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3053 4139 5349 3053 Size: 1086 1210 3951 Fragments arranged by size: 3951 1210 1086 BclI T'GATC_A Cuts at: 1548 1548 Size: 6247 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4858 136 Size: 107 122 71 631 605 3186 1525 Fragments arranged by size: 3186 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6242 6242 Size: 6247 BlpI GC'TnA_GC Cuts at: 3664 3664 Size: 6247 Bme1580I G_kGCm'C Cuts at: 1790 4308 5464 1790 Size: 2518 1156 2573 Fragments arranged by size: 2573 2518 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6247 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3978 4936 442 Size: 416 53 83 399 24 2561 958 1753 Fragments arranged by size: 2561 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6247 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3549 4907 1239 Size: 2310 1358 2579 Fragments arranged by size: 2579 2310 1358 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4372 5237 5535 4372 Size: 865 298 5084 Fragments arranged by size: 5084 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4910 882 Size: 617 3411 2219 Fragments arranged by size: 3411 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3544 4038 493 Size: 711 2340 494 2702 Fragments arranged by size: 2702 2340 711 494 BsaBI GATnn'nnATC Cuts at: 2154 6241 2154 Size: 4087 2160 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3256 4054 4436 5071 Size: 53 83 186 936 1723 798 382 635 Cuts at: 5071 275 Size: 1451 Fragments arranged by size: 1723 1451 936 798 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3204 3482 4680 5078 5352 5499 5910 3204 Size: 278 1198 398 274 147 411 3541 Fragments arranged by size: 3541 1198 411 398 278 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6022 Fragments arranged by size: 6022 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6247 BseYI C'CCAG_C Cuts at: 5450 5946 5450 Size: 496 5751 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5674 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3697 4458 5486 1540 Size: 275 186 1696 761 1028 2301 Fragments arranged by size: 2301 1696 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4308 4393 5464 4308 Size: 85 1071 5091 Fragments arranged by size: 5091 1071 85 BsmI GAATG_Cn' Cuts at: 3758 3851 3758 Size: 93 6154 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3566 4308 4393 5464 1391 Size: 399 1776 742 85 1071 2174 Fragments arranged by size: 2174 1776 1071 742 399 85 BspEI T'CCGG_A Cuts at: 3204 5910 3204 Size: 2706 3541 BspHI T'CATG_A Cuts at: 4136 5866 4136 Size: 1730 4517 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2836 3602 844 Size: 1992 766 3489 Fragments arranged by size: 3489 1992 766 BsrBI CCG'CTC Cuts at: 1394 1823 4134 1394 Size: 429 2311 3507 Fragments arranged by size: 3507 2311 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2272 4742 4916 65 Size: 1183 1024 2470 174 1396 Fragments arranged by size: 2470 1396 1183 1024 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4891 5078 1700 Size: 110 3081 187 2869 Fragments arranged by size: 3081 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3430 96 Size: 3334 2913 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6247 BssSI C'ACGA_G Cuts at: 4307 5319 4307 Size: 1012 5235 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6247 BstBI TT'CG_AA Cuts at: 2007 5051 2007 Size: 3044 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6247 BstYI r'GATC_y Cuts at: 1972 2155 3577 3944 4329 4346 5798 5884 5896 Size: 183 1422 367 385 17 1452 86 12 Cuts at: 5896 6242 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1422 385 367 346 183 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2307 513 Size: 552 1242 4453 Fragments arranged by size: 4453 1242 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5508 Fragments arranged by size: 5508 683 56 BtsI GCAGTG_nn' Cuts at: 2287 3763 4557 4577 2287 Size: 1476 794 20 3957 Fragments arranged by size: 3957 1476 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6247 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6212 DraI TTT'AAA Cuts at: 3551 3551 Size: 6247 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2353 5254 809 Size: 507 1037 2901 1802 Fragments arranged by size: 2901 1802 1037 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3535 3694 4583 Size: 54 1425 50 275 18 1705 159 889 Cuts at: 4583 6212 8 Size: 1629 43 Fragments arranged by size: 1705 1629 1425 889 275 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3694 1537 Size: 275 1882 4090 Fragments arranged by size: 4090 1882 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2525 3115 4177 1353 Size: 1172 590 1062 3423 Fragments arranged by size: 3423 1172 1062 590 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4834 5208 5354 387 Size: 1064 138 3245 374 146 1280 Fragments arranged by size: 3245 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3549 4310 4907 5694 5962 1239 Size: 393 1917 761 597 787 268 1524 Fragments arranged by size: 1917 1524 787 761 597 393 268 EcoRI G'AATT_C Cuts at: 3556 3934 3556 Size: 378 5869 EcoRV GAT'ATC Cuts at: 3331 3331 Size: 6247 FspI TGC'GCA Cuts at: 4753 4753 Size: 6247 HaeII r_GCGC'y Cuts at: 1678 5394 1678 Size: 3716 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4888 4920 4962 4994 1373 Size: 32 135 32 3316 32 42 32 2626 Fragments arranged by size: 3316 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3591 3837 669 Size: 988 281 1653 246 3079 Fragments arranged by size: 3079 1653 988 281 246 HindIII A'AGCT_T Cuts at: 748 2462 748 Size: 1714 4533 HpaI GTT'AAC Cuts at: 3837 3837 Size: 6247 KpnI G_GTAC'C Cuts at: 3572 3572 Size: 6247 MfeI C'AATT_G Cuts at: 2429 3846 2429 Size: 1417 4830 MluI A'CGCG_T Cuts at: 6123 6123 Size: 6247 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2112 2437 5361 5545 1305 Size: 807 325 2924 184 2007 Fragments arranged by size: 2924 2007 807 325 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3537 10 Size: 54 1425 343 1705 2720 Fragments arranged by size: 2720 1705 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3272 4205 4564 4723 518 Size: 648 2106 933 359 159 2042 Fragments arranged by size: 2106 2042 933 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3663 4340 5488 5733 1606 Size: 2057 677 1148 245 2120 Fragments arranged by size: 2120 2057 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2307 513 Size: 552 1242 4453 Fragments arranged by size: 4453 1242 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6247 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6247 NotI GC'GGCC_GC Cuts at: 3694 3694 Size: 6247 NspI r_CATG'y Cuts at: 1132 1229 3083 3605 5150 1132 Size: 97 1854 522 1545 2229 Fragments arranged by size: 2229 1854 1545 522 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6247 PciI A'CATG_T Cuts at: 3079 5146 3079 Size: 2067 4180 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6247 PmeI GTTT'AAAC Cuts at: 3551 3551 Size: 6247 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4316 4348 5829 5861 4316 Size: 32 1481 32 4702 Fragments arranged by size: 4702 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6247 PsiI TTA'TAA Cuts at: 2336 3817 2336 Size: 1481 4766 PstI C_TGCA'G Cuts at: 830 3358 3599 830 Size: 2528 241 3478 Fragments arranged by size: 3478 2528 241 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6247 SalI G'TCGA_C Cuts at: 1936 3589 1936 Size: 1653 4594 SbfI CC_TGCA'GG Cuts at: 3599 3599 Size: 6247 ScaI AGT'ACT Cuts at: 4495 6207 4495 Size: 1712 4535 SfcI C'TryA_G Cuts at: 826 1045 2080 3354 3595 4730 5411 5602 826 Size: 219 1035 1274 241 1135 681 191 1471 Fragments arranged by size: 1471 1274 1135 1035 681 241 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6247 SmaI CCC'GGG Cuts at: 1872 3574 1872 Size: 1702 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4351 5252 5514 820 Size: 197 926 2408 901 262 1553 Fragments arranged by size: 2408 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3544 4038 493 Size: 3051 494 2702 Fragments arranged by size: 3051 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6247 SphI G_CATG'C Cuts at: 1132 3605 1132 Size: 2473 3774 SspI AAT'ATT Cuts at: 5 52 2361 4171 5 Size: 47 2309 1810 2081 Fragments arranged by size: 2309 2081 1810 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6247 StyI C'CwwG_G Cuts at: 513 1065 2290 2307 3686 513 Size: 552 1225 17 1379 3074 Fragments arranged by size: 3074 1379 1225 552 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4274 4291 4444 4629 5074 325 Size: 1255 2694 17 153 185 445 1498 Fragments arranged by size: 2694 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3430 3469 4493 Size: 274 80 33 51 1443 1453 39 1024 Cuts at: 4493 6205 96 Size: 1712 138 Fragments arranged by size: 1712 1453 1443 1024 274 138 80 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6207 6239 6207 Size: 32 6215 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6247 XbaI T'CTAG_A Cuts at: 3583 3583 Size: 6247 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6247 XmaI C'CCGG_G Cuts at: 1870 3572 1870 Size: 1702 4545 XmnI GAAnn'nnTTC Cuts at: 3096 4376 3096 Size: 1280 4967 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4055 276 Size: 53 83 186 936 2521 2468 Fragments arranged by size: 2521 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI