(Circular) (Six-base) MAPSORT of: pFN21ASDA1368.seq Check: 7137 from: 1 to: 7870 pFN21ASDA1368 7870 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2846 5680 278 Size: 53 83 186 936 1310 2834 2468 Fragments arranged by size: 2834 2468 1310 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2334 4083 5213 1937 Size: 397 1749 1130 4594 Fragments arranged by size: 4594 1749 1130 397 Acc65I G'GTAC_C Cuts at: 5191 5191 Size: 7870 AclI AA'CG_TT Cuts at: 1563 2541 2963 5997 6370 1563 Size: 978 422 3034 373 3063 Fragments arranged by size: 3063 3034 978 422 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2713 2873 5093 5933 7317 7585 1632 Size: 1081 160 2220 840 1384 268 1917 Fragments arranged by size: 2220 1917 1384 1081 840 268 160 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7673 AflIII A'CryG_T Cuts at: 1203 6769 7746 1203 Size: 5566 977 1327 Fragments arranged by size: 5566 1327 977 AgeI A'CCGG_T Cuts at: 4766 6701 4766 Size: 1935 5935 AhdI GACnn_n'nnGTC Cuts at: 6599 6599 Size: 7870 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7870 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7870 AlwNI CAG_nnn'CTG Cuts at: 2585 2879 3092 3308 5613 7185 2585 Size: 294 213 216 2305 1572 3270 Fragments arranged by size: 3270 2305 1572 294 216 213 ApaLI G'TGCA_C Cuts at: 4985 5927 7083 4985 Size: 942 1156 5772 Fragments arranged by size: 5772 1156 942 ApoI r'AATT_y Cuts at: 1493 2286 2495 5179 5405 5557 7666 1493 Size: 793 209 2684 226 152 2109 1697 Fragments arranged by size: 2684 2109 1697 793 226 209 152 AseI AT'TA_AT Cuts at: 160 1032 6424 160 Size: 872 5392 1606 Fragments arranged by size: 5392 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7870 AvaI C'yCGr_G Cuts at: 1870 1943 4069 4744 5023 5195 1870 Size: 73 2126 675 279 172 4545 Fragments arranged by size: 4545 2126 675 279 172 73 BamHI G'GATC_C Cuts at: 5200 5567 5200 Size: 367 7503 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3641 5191 6646 618 Size: 325 217 627 1854 1550 1455 1842 Fragments arranged by size: 1854 1842 1550 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4146 4166 4460 5189 1391 Size: 2755 20 294 729 4072 Fragments arranged by size: 4072 2755 729 294 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3477 3933 4057 928 Size: 971 1578 456 124 4741 Fragments arranged by size: 4741 1578 971 456 124 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6061 6095 1903 Size: 34 4124 34 3678 Fragments arranged by size: 4124 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2145 2464 5762 6972 2145 Size: 319 3298 1210 3043 Fragments arranged by size: 3298 3043 1210 319 BclI T'GATC_A Cuts at: 1548 2208 4096 1548 Size: 660 1888 5322 Fragments arranged by size: 5322 1888 660 BfrBI ATG'CAT Cuts at: 2414 2414 Size: 7870 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4595 6481 136 Size: 107 122 71 631 605 2923 1886 1525 Fragments arranged by size: 2923 1886 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7865 7865 Size: 7870 BlpI GC'TnA_GC Cuts at: 4187 5287 4187 Size: 1100 6770 Bme1580I G_kGCm'C Cuts at: 1790 3231 4989 5931 7087 1790 Size: 1441 1758 942 1156 2573 Fragments arranged by size: 2573 1758 1441 1156 942 BmgBI CAC'GTC Cuts at: 1318 4114 1318 Size: 2796 5074 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3051 5601 6559 Size: 416 53 83 399 24 1634 2550 958 Cuts at: 6559 442 Size: 1753 Fragments arranged by size: 2550 1753 1634 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7870 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2849 2894 2901 3440 3589 4622 4643 4900 Size: 1610 45 7 539 149 1033 21 257 Cuts at: 4900 6530 1239 Size: 1630 2579 Fragments arranged by size: 2579 1630 1610 1033 539 257 149 45 21 7 Bpu10I CC'TnA_GC Cuts at: 2787 3108 2787 Size: 321 7549 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3837 5995 6860 7158 3837 Size: 2158 865 298 4549 Fragments arranged by size: 4549 2158 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3603 6533 882 Size: 617 2104 2930 2219 Fragments arranged by size: 2930 2219 2104 617 BsaAI yAC'GTr Cuts at: 493 1204 4561 5167 5661 493 Size: 711 3357 606 494 2702 Fragments arranged by size: 3357 2702 711 606 494 BsaBI GATnn'nnATC Cuts at: 2207 2933 7864 2207 Size: 726 4931 2213 Fragments arranged by size: 4931 2213 726 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2843 5677 6059 6694 Size: 53 83 186 936 1310 2834 382 635 Cuts at: 6694 275 Size: 1451 Fragments arranged by size: 2834 1451 1310 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2137 2616 4766 6303 6701 6975 7122 7533 2137 Size: 479 2150 1537 398 274 147 411 2474 Fragments arranged by size: 2474 2150 1537 479 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2822 2852 4877 4907 1178 Size: 30 165 30 1419 30 2025 30 4141 Fragments arranged by size: 4141 2025 1419 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3437 3753 4789 4840 4885 1186 Size: 2251 316 1036 51 45 4171 Fragments arranged by size: 4171 2251 1036 316 51 45 BseYI C'CCAG_C Cuts at: 2069 3308 3824 4382 4499 4511 4995 5033 7073 Size: 1239 516 558 117 12 484 38 2040 Cuts at: 7073 7569 2069 Size: 496 2370 Fragments arranged by size: 2370 2040 1239 558 516 496 484 117 38 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4145 4417 1163 Size: 573 2409 272 4616 Fragments arranged by size: 4616 2409 573 272 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5320 6081 7109 1540 Size: 275 186 3319 761 1028 2301 Fragments arranged by size: 3319 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4146 4989 5931 6016 7087 4146 Size: 843 942 85 1071 4929 Fragments arranged by size: 4929 1071 942 843 85 BsiWI C'GTAC_G Cuts at: 5078 5078 Size: 7870 BsmI GAATG_Cn' Cuts at: 3805 3885 5381 5474 3805 Size: 80 1496 93 6201 Fragments arranged by size: 6201 1496 93 80 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3231 4146 4166 4460 4989 5189 5931 Size: 399 1441 915 20 294 529 200 742 Cuts at: 5931 6016 7087 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1441 1071 915 742 529 399 294 200 85 20 BspEI T'CCGG_A Cuts at: 7533 7533 Size: 7870 BspHI T'CATG_A Cuts at: 2211 5759 7489 2211 Size: 3548 1730 2592 Fragments arranged by size: 3548 2592 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4488 5225 844 Size: 3644 737 3489 Fragments arranged by size: 3644 3489 737 BsrBI CCG'CTC Cuts at: 1394 1823 3211 5757 1394 Size: 429 1388 2546 3507 Fragments arranged by size: 3507 2546 1388 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2229 3024 4029 6365 6539 65 Size: 1183 981 795 1005 2336 174 1396 Fragments arranged by size: 2336 1396 1183 1005 981 795 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3561 4766 6514 6701 1700 Size: 110 1751 1205 1748 187 2869 Fragments arranged by size: 2869 1751 1748 1205 187 110 BsrGI T'GTAC_A Cuts at: 96 5159 96 Size: 5063 2807 BssHII G'CGCG_C Cuts at: 1925 2858 1925 Size: 933 6937 BssSI C'ACGA_G Cuts at: 4849 5930 6942 4849 Size: 1081 1012 5777 Fragments arranged by size: 5777 1081 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2552 3005 794 Size: 1758 453 5659 Fragments arranged by size: 5659 1758 453 BstBI TT'CG_AA Cuts at: 2007 6674 2007 Size: 4667 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7870 BstXI CCAn_nnnn'nTGG Cuts at: 4519 4931 5003 4519 Size: 412 72 7386 Fragments arranged by size: 7386 412 72 BstYI r'GATC_y Cuts at: 1972 2813 5200 5567 5952 5969 7421 7507 7519 Size: 841 2387 367 385 17 1452 86 12 Cuts at: 7519 7865 1972 Size: 346 1977 Fragments arranged by size: 2387 1977 1452 841 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2697 2757 3248 4387 4452 4473 4669 Size: 552 1632 60 491 1139 65 21 196 Cuts at: 4669 513 Size: 3714 Fragments arranged by size: 3714 1632 1139 552 491 196 65 60 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7131 Fragments arranged by size: 7131 683 56 BtsI GCAGTG_nn' Cuts at: 2054 2748 3231 3300 3942 5386 6180 6200 2054 Size: 694 483 69 642 1444 794 20 3724 Fragments arranged by size: 3724 1444 794 694 642 483 69 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7870 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7835 DraI TTT'AAA Cuts at: 5174 5174 Size: 7870 DraIII CAC_nnn'GTG Cuts at: 3331 3331 Size: 7870 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6877 809 Size: 507 5561 1802 Fragments arranged by size: 5561 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2508 3375 3559 Size: 54 1425 50 275 18 678 867 184 Cuts at: 3559 4026 4287 5005 5317 6206 7835 8 Size: 467 261 718 312 889 1629 43 Fragments arranged by size: 1629 1425 889 867 718 678 467 312 275 261 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5317 1537 Size: 275 3505 4090 Fragments arranged by size: 4090 3505 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4807 5800 1353 Size: 3454 993 3423 Fragments arranged by size: 3454 3423 993 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6457 6831 6977 387 Size: 1064 138 4868 374 146 1280 Fragments arranged by size: 4868 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4144 4144 Size: 7870 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2713 2849 2873 2894 2901 3440 3589 Size: 393 1081 136 24 21 7 539 149 Cuts at: 3589 4622 4643 4900 5093 5933 6530 7317 7585 Size: 1033 21 257 193 840 597 787 268 Cuts at: 7585 1239 Size: 1524 Fragments arranged by size: 1524 1081 1033 840 787 597 539 393 268 257 193 149 136 24 21 21 7 EcoO109I rG'GnC_Cy Cuts at: 4739 4739 Size: 7870 EcoRI G'AATT_C Cuts at: 2495 5179 5557 2495 Size: 2684 378 4808 Fragments arranged by size: 4808 2684 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2878 2878 Size: 7870 FspI TGC'GCA Cuts at: 6376 6376 Size: 7870 HaeII r_GCGC'y Cuts at: 1678 4601 7017 1678 Size: 2923 2416 2531 Fragments arranged by size: 2923 2531 2416 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2700 2732 4196 4228 6511 Size: 32 135 32 1128 32 1464 32 2283 Cuts at: 6511 6543 6585 6617 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2283 1464 1128 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4353 4816 5214 5460 669 Size: 988 281 2415 463 398 246 3079 Fragments arranged by size: 3079 2415 988 463 398 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7870 HpaI GTT'AAC Cuts at: 5460 5460 Size: 7870 KpnI G_GTAC'C Cuts at: 5195 5195 Size: 7870 MfeI C'AATT_G Cuts at: 5469 5469 Size: 7870 MluI A'CGCG_T Cuts at: 7746 7746 Size: 7870 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6984 7168 1305 Size: 5679 184 2007 Fragments arranged by size: 5679 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2510 3377 4028 5007 10 Size: 54 1425 343 678 867 651 979 2873 Fragments arranged by size: 2873 1425 979 867 678 651 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2198 2364 3488 4982 5138 5828 6187 Size: 648 1032 166 1124 1494 156 690 359 Cuts at: 6187 6346 518 Size: 159 2042 Fragments arranged by size: 2042 1494 1124 1032 690 648 359 166 159 156 MspA1I CmG'CkG Cuts at: 1606 2502 3305 3496 4006 4191 4389 4723 5286 Size: 896 803 191 510 185 198 334 563 Cuts at: 5286 5963 7111 7356 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 896 803 677 563 510 334 245 198 191 185 NcoI C'CATG_G Cuts at: 513 1065 2757 3248 4452 4669 513 Size: 552 1692 491 1204 217 3714 Fragments arranged by size: 3714 1692 1204 552 491 217 NdeI CA'TA_TG Cuts at: 387 2521 387 Size: 2134 5736 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7870 NotI GC'GGCC_GC Cuts at: 5317 5317 Size: 7870 NsiI A_TGCA'T Cuts at: 2416 2416 Size: 7870 NspI r_CATG'y Cuts at: 1132 1229 2343 2537 3019 3493 4447 5228 6773 Size: 97 1114 194 482 474 954 781 1545 Cuts at: 6773 1132 Size: 2229 Fragments arranged by size: 2229 1545 1114 954 781 482 474 194 97 PasI CC'CwG_GG Cuts at: 1348 2990 4735 1348 Size: 1642 1745 4483 Fragments arranged by size: 4483 1745 1642 PciI A'CATG_T Cuts at: 6769 6769 Size: 7870 PflMI CCAn_nnn'nTGG Cuts at: 1108 3620 1108 Size: 2512 5358 PfoI T'CCnGG_A Cuts at: 2826 2878 3417 2826 Size: 52 539 7279 Fragments arranged by size: 7279 539 52 PmeI GTTT'AAAC Cuts at: 5174 5174 Size: 7870 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5939 5971 7452 7484 5939 Size: 32 1481 32 6325 Fragments arranged by size: 6325 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4739 4739 Size: 7870 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7870 PsiI TTA'TAA Cuts at: 2979 5440 2979 Size: 2461 5409 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3512 3544 3512 Size: 32 7838 PstI C_TGCA'G Cuts at: 830 2456 3001 3650 4368 5222 830 Size: 1626 545 649 718 854 3478 Fragments arranged by size: 3478 1626 854 718 649 545 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7870 PvuII CAG'CTG Cuts at: 3305 3496 4006 3305 Size: 191 510 7169 Fragments arranged by size: 7169 510 191 RsrII CG'GwC_CG Cuts at: 2646 2646 Size: 7870 SacI G_AGCT'C Cuts at: 4146 4146 Size: 7870 SacII CC_GC'GG Cuts at: 4390 4390 Size: 7870 SalI G'TCGA_C Cuts at: 1936 5212 1936 Size: 3276 4594 SanDI GG'GwC_CC Cuts at: 4739 4739 Size: 7870 SbfI CC_TGCA'GG Cuts at: 5222 5222 Size: 7870 ScaI AGT'ACT Cuts at: 6118 7830 6118 Size: 1712 6158 SfcI C'TryA_G Cuts at: 826 1045 2452 2997 3646 4364 5218 6353 7034 Size: 219 1407 545 649 718 854 1135 681 Cuts at: 7034 7225 826 Size: 191 1471 Fragments arranged by size: 1471 1407 1135 854 718 681 649 545 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7870 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7870 SmaI CCC'GGG Cuts at: 1872 4746 5197 1872 Size: 2874 451 4545 Fragments arranged by size: 4545 2874 451 SmlI C'TyrA_G Cuts at: 820 1017 1943 3852 5023 5974 6875 7137 820 Size: 197 926 1909 1171 951 901 262 1553 Fragments arranged by size: 1909 1553 1171 951 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5167 5661 493 Size: 4674 494 2702 Fragments arranged by size: 4674 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7870 SphI G_CATG'C Cuts at: 1132 3493 5228 1132 Size: 2361 1735 3774 Fragments arranged by size: 3774 2361 1735 SspI AAT'ATT Cuts at: 5 52 5794 5 Size: 47 5742 2081 Fragments arranged by size: 5742 2081 47 StuI AGG'CCT Cuts at: 1517 4730 1517 Size: 3213 4657 StyI C'CwwG_G Cuts at: 513 1065 2757 3248 3556 4452 4669 5309 513 Size: 552 1692 491 308 896 217 640 3074 Fragments arranged by size: 3074 1692 896 640 552 491 308 217 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3676 4163 5897 5914 6067 6252 6697 Size: 1255 2096 487 1734 17 153 185 445 Cuts at: 6697 325 Size: 1498 Fragments arranged by size: 2096 1734 1498 1255 487 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5159 6116 7828 Size: 274 80 33 51 1443 3182 957 1712 Cuts at: 7828 96 Size: 138 Fragments arranged by size: 3182 1712 1443 957 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2822 2854 4787 4819 7830 7862 2822 Size: 32 1933 32 3011 32 2830 Fragments arranged by size: 3011 2830 1933 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2606 5062 1316 Size: 1290 2456 4124 Fragments arranged by size: 4124 2456 1290 XbaI T'CTAG_A Cuts at: 2318 5206 2318 Size: 2888 4982 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3292 3621 3292 Size: 329 7541 XhoI C'TCGA_G Cuts at: 1943 5023 1943 Size: 3080 4790 XmaI C'CCGG_G Cuts at: 1870 4744 5195 1870 Size: 2874 451 4545 Fragments arranged by size: 4545 2874 451 XmnI GAAnn'nnTTC Cuts at: 3102 5999 3102 Size: 2897 4973 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2844 5678 276 Size: 53 83 186 936 1310 2834 2468 Fragments arranged by size: 2834 2468 1310 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI SapI SexAI SfiI SfoI SrfI SwaI