(Circular) (Six-base) MAPSORT of: pFN21ASDA1355.seq Check: 1923 from: 1 to: 8266 pFN21ASDA1355 8266 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2645 2645 Size: 8266 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6076 278 Size: 53 83 186 936 4540 2468 Fragments arranged by size: 4540 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2631 3525 4182 5609 1937 Size: 694 894 657 1427 4594 Fragments arranged by size: 4594 1427 894 694 657 Acc65I G'GTAC_C Cuts at: 3227 5587 3227 Size: 2360 5906 AclI AA'CG_TT Cuts at: 1563 6393 6766 1563 Size: 4830 373 3063 Fragments arranged by size: 4830 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2397 3790 4454 5382 6329 7713 7981 1632 Size: 765 1393 664 928 947 1384 268 1917 Fragments arranged by size: 1917 1393 1384 947 928 765 664 268 AfeI AGC'GCT Cuts at: 5113 5113 Size: 8266 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8069 AflIII A'CryG_T Cuts at: 1203 7165 8142 1203 Size: 5962 977 1327 Fragments arranged by size: 5962 1327 977 AgeI A'CCGG_T Cuts at: 7097 7097 Size: 8266 AhdI GACnn_n'nnGTC Cuts at: 2702 3694 5528 6995 2702 Size: 992 1834 1467 3973 Fragments arranged by size: 3973 1834 1467 992 AleI CACnn'nnGTG Cuts at: 1166 2924 3560 5264 1166 Size: 1758 636 1704 4168 Fragments arranged by size: 4168 1758 1704 636 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2531 4217 1184 Size: 1347 1686 5233 Fragments arranged by size: 5233 1686 1347 AlwNI CAG_nnn'CTG Cuts at: 2473 2963 3645 3710 4474 4660 4781 4801 5193 Size: 490 682 65 764 186 121 20 392 Cuts at: 5193 6009 7581 2473 Size: 816 1572 3158 Fragments arranged by size: 3158 1572 816 764 682 490 392 186 121 65 20 ApaI G_GGCC'C Cuts at: 3240 4568 4593 3240 Size: 1328 25 6913 Fragments arranged by size: 6913 1328 25 ApaLI G'TGCA_C Cuts at: 6323 7479 6323 Size: 1156 7110 ApoI r'AATT_y Cuts at: 1493 5294 5465 5575 5801 5953 8062 1493 Size: 3801 171 110 226 152 2109 1697 Fragments arranged by size: 3801 2109 1697 226 171 152 110 AseI AT'TA_AT Cuts at: 160 1032 6820 160 Size: 872 5788 1606 Fragments arranged by size: 5788 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8266 AvaI C'yCGr_G Cuts at: 1870 1943 2850 3501 3860 3930 5073 5591 1870 Size: 73 907 651 359 70 1143 518 4545 Fragments arranged by size: 4545 1143 907 651 518 359 73 70 BamHI G'GATC_C Cuts at: 2598 2859 3952 4445 4486 4516 5596 5963 2598 Size: 261 1093 493 41 30 1080 367 4901 Fragments arranged by size: 4901 1093 1080 493 367 261 41 30 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2031 2713 3227 3587 4069 Size: 325 217 627 244 682 514 360 482 Cuts at: 4069 4702 4741 5587 7042 618 Size: 633 39 846 1455 1842 Fragments arranged by size: 1842 1455 846 682 633 627 514 482 360 325 244 217 39 BanII G_rGCy'C Cuts at: 1391 3240 4406 4554 4568 4593 4863 5004 5506 Size: 1849 1166 148 14 25 270 141 502 Cuts at: 5506 5585 1391 Size: 79 4072 Fragments arranged by size: 4072 1849 1166 502 270 148 141 79 25 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2331 2385 2818 4731 5393 928 Size: 971 432 54 433 1913 662 3801 Fragments arranged by size: 3801 1913 971 662 433 432 54 BbvCI CC'TCA_GC Cuts at: 2051 2772 2956 4218 5165 2051 Size: 721 184 1262 947 5152 Fragments arranged by size: 5152 1262 947 721 184 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6457 6491 1903 Size: 34 4520 34 3678 Fragments arranged by size: 4520 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4885 4957 6158 7368 4885 Size: 72 1201 1210 5783 Fragments arranged by size: 5783 1210 1201 72 BclI T'GATC_A Cuts at: 1548 3162 1548 Size: 1614 6652 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3506 4008 6877 Size: 107 122 71 631 605 1834 502 2869 Cuts at: 6877 136 Size: 1525 Fragments arranged by size: 2869 1834 1525 631 605 502 122 107 71 BglII A'GATC_T Cuts at: 8261 8261 Size: 8266 BlpI GC'TnA_GC Cuts at: 5367 5683 5367 Size: 316 7950 Bme1580I G_kGCm'C Cuts at: 1790 2718 2931 3149 3240 3471 3539 4568 4593 Size: 928 213 218 91 231 68 1029 25 Cuts at: 4593 4705 6327 7483 1790 Size: 112 1622 1156 2573 Fragments arranged by size: 2573 1622 1156 1029 928 231 218 213 112 91 68 25 BmgBI CAC'GTC Cuts at: 1318 2529 1318 Size: 1211 7055 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2696 3481 3700 Size: 416 53 83 399 24 1279 785 219 Cuts at: 3700 3751 4445 4689 4758 5997 6955 442 Size: 51 694 244 69 1239 958 1753 Fragments arranged by size: 1753 1279 1239 958 785 694 416 399 244 219 83 69 53 51 24 BmtI G_CTAG'C Cuts at: 1056 3779 1056 Size: 2723 5543 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5099 5305 5099 Size: 206 8060 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2285 2495 2632 4196 4424 4568 4632 4734 Size: 1046 210 137 1564 228 144 64 102 Cuts at: 4734 4778 4914 5160 5339 5376 6926 1239 Size: 44 136 246 179 37 1550 2579 Fragments arranged by size: 2579 1564 1550 1046 246 228 210 179 144 137 136 102 64 44 37 Bpu10I CC'TnA_GC Cuts at: 2051 2772 2956 4218 4405 4802 5165 2051 Size: 721 184 1262 187 397 363 5152 Fragments arranged by size: 5152 1262 721 397 363 187 184 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3248 4874 6391 7256 7554 3248 Size: 1626 1517 865 298 3960 Fragments arranged by size: 3960 1626 1517 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2318 6929 882 Size: 617 819 4611 2219 Fragments arranged by size: 4611 2219 819 617 BsaAI yAC'GTr Cuts at: 493 1204 2266 3529 5563 6057 493 Size: 711 1062 1263 2034 494 2702 Fragments arranged by size: 2702 2034 1263 1062 711 494 BsaBI GATnn'nnATC Cuts at: 8260 8260 Size: 8266 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6073 6455 7090 275 Size: 53 83 186 936 4540 382 635 1451 Fragments arranged by size: 4540 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3829 4169 6699 7097 7371 7518 7929 3829 Size: 340 2530 398 274 147 411 4166 Fragments arranged by size: 4166 2530 411 398 340 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4254 4284 4776 4806 1178 Size: 30 165 30 2851 30 492 30 4638 Fragments arranged by size: 4638 2851 492 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3351 4871 5266 5411 1186 Size: 2165 1520 395 145 4041 Fragments arranged by size: 4041 2165 1520 395 145 BseYI C'CCAG_C Cuts at: 2120 2137 2679 2895 2930 2986 3274 3532 3790 Size: 17 542 216 35 56 288 258 258 Cuts at: 3790 4020 4142 4457 4502 4653 4707 4862 4985 Size: 230 122 315 45 151 54 155 123 Cuts at: 4985 5193 7469 7965 2120 Size: 208 2276 496 2421 Fragments arranged by size: 2421 2276 542 496 315 288 258 258 230 216 208 155 151 123 122 56 54 45 35 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2486 2600 3190 3840 4371 4857 1163 Size: 573 750 114 590 650 531 486 4572 Fragments arranged by size: 4572 750 650 590 573 531 486 114 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2083 2163 2167 4374 5716 6477 Size: 275 186 82 80 4 2207 1342 761 Cuts at: 6477 7505 1540 Size: 1028 2301 Fragments arranged by size: 2301 2207 1342 1028 761 275 186 82 80 4 BsiHKAI G_wGCw'C Cuts at: 2982 6327 6412 7483 2982 Size: 3345 85 1071 3765 Fragments arranged by size: 3765 3345 1071 85 BsmI GAATG_Cn' Cuts at: 3482 3684 5777 5870 3482 Size: 202 2093 93 5878 Fragments arranged by size: 5878 2093 202 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4165 4165 Size: 8266 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2718 2931 2982 3149 3240 3471 3539 Size: 399 928 213 51 167 91 231 68 Cuts at: 3539 4406 4554 4568 4593 4705 4863 5004 5506 Size: 867 148 14 25 112 158 141 502 Cuts at: 5506 5585 6327 6412 7483 1391 Size: 79 742 85 1071 2174 Fragments arranged by size: 2174 1071 928 867 742 502 399 231 213 167 158 148 141 112 91 85 79 68 51 25 14 BspEI T'CCGG_A Cuts at: 3829 7929 3829 Size: 4100 4166 BspHI T'CATG_A Cuts at: 6155 7885 6155 Size: 1730 6536 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2157 2645 5621 844 Size: 1313 488 2976 3489 Fragments arranged by size: 3489 2976 1313 488 BsrBI CCG'CTC Cuts at: 1394 1823 3289 5041 6153 1394 Size: 429 1466 1752 1112 3507 Fragments arranged by size: 3507 1752 1466 1112 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2941 3496 6761 6935 65 Size: 1183 1693 555 3265 174 1396 Fragments arranged by size: 3265 1693 1396 1183 555 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2162 2166 2909 4002 4232 4289 4305 Size: 110 352 4 743 1093 230 57 16 Cuts at: 4305 6910 7097 1700 Size: 2605 187 2869 Fragments arranged by size: 2869 2605 1093 743 352 230 187 110 57 16 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8266 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8266 BssSI C'ACGA_G Cuts at: 4109 6326 7338 4109 Size: 2217 1012 5037 Fragments arranged by size: 5037 2217 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2783 4394 794 Size: 1989 1611 4666 Fragments arranged by size: 4666 1989 1611 BstBI TT'CG_AA Cuts at: 2007 7070 2007 Size: 5063 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8266 BstXI CCAn_nnnn'nTGG Cuts at: 5099 5429 5099 Size: 330 7936 BstYI r'GATC_y Cuts at: 1972 2598 2859 3952 4348 4445 4486 4516 5596 Size: 626 261 1093 396 97 41 30 1080 Cuts at: 5596 5963 6348 6365 7817 7903 7915 8261 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1093 1080 626 396 385 367 346 261 97 86 41 30 17 12 Bsu36I CC'TnA_GG Cuts at: 2094 5436 2094 Size: 3342 4924 BtgI C'CryG_G Cuts at: 513 1065 2022 2503 3463 3567 3902 513 Size: 552 957 481 960 104 335 4877 Fragments arranged by size: 4877 960 957 552 481 335 104 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3151 487 Size: 683 56 1925 5602 Fragments arranged by size: 5602 1925 683 56 BtsI GCAGTG_nn' Cuts at: 3074 3487 3711 3924 4992 5104 5415 5782 6576 Size: 413 224 213 1068 112 311 367 794 Cuts at: 6576 6596 3074 Size: 20 4744 Fragments arranged by size: 4744 1068 794 413 367 311 224 213 112 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8266 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8231 DraI TTT'AAA Cuts at: 5570 5570 Size: 8266 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4378 7273 809 Size: 507 3062 2895 1802 Fragments arranged by size: 3062 2895 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2160 2164 Size: 54 1425 50 275 18 213 117 4 Cuts at: 2164 2554 2889 3507 3660 4152 4230 4266 4287 Size: 390 335 618 153 492 78 36 21 Cuts at: 4287 5340 5713 6602 8231 8 Size: 1053 373 889 1629 43 Fragments arranged by size: 1629 1425 1053 889 618 492 390 373 335 275 213 153 117 78 54 50 43 36 21 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2160 2164 5713 1537 Size: 275 348 4 3549 4090 Fragments arranged by size: 4090 3549 348 275 4 EarI CTCTTCn'nnn_ Cuts at: 1353 6196 1353 Size: 4843 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2591 3334 4094 4260 4331 5468 Size: 1064 138 1002 743 760 166 71 1137 Cuts at: 5468 6853 7227 7373 387 Size: 1385 374 146 1280 Fragments arranged by size: 1385 1280 1137 1064 1002 760 743 374 166 146 138 71 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2285 2397 2495 2632 3790 4196 4424 Size: 393 653 112 98 137 1158 406 228 Cuts at: 4424 4454 4568 4632 4734 4778 4914 5160 5339 Size: 30 114 64 102 44 136 246 179 Cuts at: 5339 5376 5382 6329 6926 7713 7981 1239 Size: 37 6 947 597 787 268 1524 Fragments arranged by size: 1524 1158 947 787 653 597 406 393 268 246 228 179 137 136 114 112 102 98 64 44 37 30 6 EcoNI CCTnn'n_nnAGG Cuts at: 2555 4082 5175 5459 2555 Size: 1527 1093 284 5362 Fragments arranged by size: 5362 1527 1093 284 EcoO109I rG'GnC_Cy Cuts at: 2292 2548 2696 3109 3237 3355 4535 4564 4589 Size: 256 148 413 128 118 1180 29 25 Cuts at: 4589 4778 2292 Size: 189 5780 Fragments arranged by size: 5780 1180 413 256 189 148 128 118 29 25 EcoRI G'AATT_C Cuts at: 5294 5465 5575 5953 5294 Size: 171 110 378 7607 Fragments arranged by size: 7607 378 171 110 FseI GG_CCGG'CC Cuts at: 2166 2170 2166 Size: 4 8262 FspI TGC'GCA Cuts at: 6772 6772 Size: 8266 HaeII r_GCGC'y Cuts at: 1678 2667 4339 4579 4903 5115 7413 1678 Size: 989 1672 240 324 212 2298 2531 Fragments arranged by size: 2531 2298 1672 989 324 240 212 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2087 2119 2296 2328 2688 Size: 32 135 32 515 32 177 32 360 Cuts at: 2688 2720 3627 3659 3680 3712 3871 3903 4254 Size: 32 907 32 21 32 159 32 351 Cuts at: 4254 4286 4437 4469 4508 4540 4776 4808 5514 Size: 32 151 32 39 32 236 32 706 Cuts at: 5514 5546 6907 6939 6981 7013 1373 Size: 32 1361 32 42 32 2626 Fragments arranged by size: 2626 1361 907 706 515 360 351 236 177 159 151 135 42 39 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 21 HincII GTy'rAC Cuts at: 669 1657 1938 5610 5856 669 Size: 988 281 3672 246 3079 Fragments arranged by size: 3672 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8266 HpaI GTT'AAC Cuts at: 5856 5856 Size: 8266 KpnI G_GTAC'C Cuts at: 3231 5591 3231 Size: 2360 5906 MfeI C'AATT_G Cuts at: 5865 5865 Size: 8266 MluI A'CGCG_T Cuts at: 8142 8142 Size: 8266 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2439 4394 7380 7564 1305 Size: 1134 1955 2986 184 2007 Fragments arranged by size: 2986 2007 1955 1134 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2556 2891 3509 3662 5342 Size: 54 1425 343 724 335 618 153 1680 Cuts at: 5342 10 Size: 2934 Fragments arranged by size: 2934 1680 1425 724 618 343 335 153 54 MslI CAynn'nnrTG Cuts at: 518 1166 2711 2924 3032 3560 5264 6224 6583 Size: 648 1545 213 108 528 1704 960 359 Cuts at: 6583 6742 518 Size: 159 2042 Fragments arranged by size: 2042 1704 1545 960 648 528 359 213 159 108 MspA1I CmG'CkG Cuts at: 1606 2683 2802 3051 3090 3118 3854 3904 4201 Size: 1077 119 249 39 28 736 50 297 Cuts at: 4201 4385 5193 5445 5682 6359 7507 7752 1606 Size: 184 808 252 237 677 1148 245 2120 Fragments arranged by size: 2120 1148 1077 808 736 677 297 252 249 245 237 184 119 50 39 28 NaeI GCC'GGC Cuts at: 2164 2168 2911 4004 4234 4291 2164 Size: 4 743 1093 230 57 6139 Fragments arranged by size: 6139 1093 743 230 57 4 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6757 Fragments arranged by size: 6757 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8266 NgoMIV G'CCGG_C Cuts at: 2162 2166 2909 4002 4232 4289 2162 Size: 4 743 1093 230 57 6139 Fragments arranged by size: 6139 1093 743 230 57 4 NheI G'CTAG_C Cuts at: 1052 3775 1052 Size: 2723 5543 NotI GC'GGCC_GC Cuts at: 5713 5713 Size: 8266 NspI r_CATG'y Cuts at: 1132 1229 2183 3037 5234 5624 7169 1132 Size: 97 954 854 2197 390 1545 2229 Fragments arranged by size: 2229 2197 1545 954 854 390 97 PasI CC'CwG_GG Cuts at: 1348 3142 3172 3190 4015 4953 5300 1348 Size: 1794 30 18 825 938 347 4314 Fragments arranged by size: 4314 1794 938 825 347 30 18 PciI A'CATG_T Cuts at: 7165 7165 Size: 8266 PflMI CCAn_nnn'nTGG Cuts at: 1108 2028 4660 4781 5193 1108 Size: 920 2632 121 412 4181 Fragments arranged by size: 4181 2632 920 412 121 PfoI T'CCnGG_A Cuts at: 2358 2436 2808 4780 5176 2358 Size: 78 372 1972 396 5448 Fragments arranged by size: 5448 1972 396 372 78 PmeI GTTT'AAAC Cuts at: 5570 5570 Size: 8266 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6335 6367 7848 7880 6335 Size: 32 1481 32 6721 Fragments arranged by size: 6721 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2548 2696 3355 4535 4778 2548 Size: 148 659 1180 243 6036 Fragments arranged by size: 6036 1180 659 243 148 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8266 PsiI TTA'TAA Cuts at: 5836 5836 Size: 8266 PspOMI G'GGCC_C Cuts at: 3236 4564 4589 3236 Size: 1328 25 6913 Fragments arranged by size: 6913 1328 25 PspXI vC'TCGA_Gb Cuts at: 5073 5073 Size: 8266 PstI C_TGCA'G Cuts at: 830 2286 2847 3117 3633 3747 4999 5152 5618 Size: 1456 561 270 516 114 1252 153 466 Cuts at: 5618 830 Size: 3478 Fragments arranged by size: 3478 1456 1252 561 516 466 270 153 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8266 PvuII CAG'CTG Cuts at: 2683 2802 3090 3118 3854 4201 4385 5193 2683 Size: 119 288 28 736 347 184 808 5756 Fragments arranged by size: 5756 808 736 347 288 184 119 28 SacII CC_GC'GG Cuts at: 3905 3905 Size: 8266 SalI G'TCGA_C Cuts at: 1936 5608 1936 Size: 3672 4594 SanDI GG'GwC_CC Cuts at: 3355 4535 3355 Size: 1180 7086 SbfI CC_TGCA'GG Cuts at: 5618 5618 Size: 8266 ScaI AGT'ACT Cuts at: 6514 8226 6514 Size: 1712 6554 SfcI C'TryA_G Cuts at: 826 1045 2282 2797 2843 3113 3629 3743 4678 Size: 219 1237 515 46 270 516 114 935 Cuts at: 4678 4995 5148 5614 6749 7430 7621 826 Size: 317 153 466 1135 681 191 1471 Fragments arranged by size: 1471 1237 1135 935 681 516 515 466 317 270 219 191 153 114 46 SfiI GGCCn_nnn'nGGCC Cuts at: 3506 3506 Size: 8266 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8266 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8266 SmaI CCC'GGG Cuts at: 1872 2852 3862 3932 5593 1872 Size: 980 1010 70 1661 4545 Fragments arranged by size: 4545 1661 1010 980 70 SmlI C'TyrA_G Cuts at: 820 1017 1943 3263 4853 5073 6370 7271 7533 Size: 197 926 1320 1590 220 1297 901 262 Cuts at: 7533 820 Size: 1553 Fragments arranged by size: 1590 1553 1320 1297 926 901 262 220 197 SnaBI TAC'GTA Cuts at: 493 5563 6057 493 Size: 5070 494 2702 Fragments arranged by size: 5070 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8266 SphI G_CATG'C Cuts at: 1132 3037 5624 1132 Size: 1905 2587 3774 Fragments arranged by size: 3774 2587 1905 SspI AAT'ATT Cuts at: 5 52 3305 6190 5 Size: 47 3253 2885 2081 Fragments arranged by size: 3253 2885 2081 47 StuI AGG'CCT Cuts at: 1517 4077 5055 1517 Size: 2560 978 4728 Fragments arranged by size: 4728 2560 978 StyI C'CwwG_G Cuts at: 513 1065 2022 2551 3096 3294 3349 3381 3453 Size: 552 957 529 545 198 55 32 72 Cuts at: 3453 3582 3699 5705 513 Size: 129 117 2006 3074 Fragments arranged by size: 3074 2006 957 552 545 529 198 129 117 72 55 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2392 2684 2693 2909 3682 3690 3770 Size: 1255 812 292 9 216 773 8 80 Cuts at: 3770 4777 6293 6310 6463 6648 7093 325 Size: 1007 1516 17 153 185 445 1498 Fragments arranged by size: 1516 1498 1255 1007 812 773 445 292 216 185 153 80 17 9 8 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6512 8224 96 Size: 274 80 33 51 1443 4535 1712 138 Fragments arranged by size: 4535 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8226 8258 8226 Size: 32 8234 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8266 XbaI T'CTAG_A Cuts at: 5602 5602 Size: 8266 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3493 3565 4171 3493 Size: 72 606 7588 Fragments arranged by size: 7588 606 72 XhoI C'TCGA_G Cuts at: 1943 5073 1943 Size: 3130 5136 XmaI C'CCGG_G Cuts at: 1870 2850 3860 3930 5591 1870 Size: 980 1010 70 1661 4545 Fragments arranged by size: 4545 1661 1010 980 70 XmnI GAAnn'nnTTC Cuts at: 3837 4962 6395 3837 Size: 1125 1433 5708 Fragments arranged by size: 5708 1433 1125 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6074 276 Size: 53 83 186 936 4540 2468 Fragments arranged by size: 4540 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacI SapI SexAI SfoI SrfI SwaI