(Circular) (Six-base) MAPSORT of: pFN21ASDA1349.seq Check: 4087 from: 1 to: 6946 pFN21ASDA1349 6946 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4756 278 Size: 53 83 186 936 3220 2468 Fragments arranged by size: 3220 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4289 1937 Size: 2352 4594 Acc65I G'GTAC_C Cuts at: 4267 4267 Size: 6946 AclI AA'CG_TT Cuts at: 1563 5073 5446 1563 Size: 3510 373 3063 Fragments arranged by size: 3510 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2094 2409 2982 3402 3910 4078 4162 5009 Size: 462 315 573 420 508 168 84 847 Cuts at: 5009 6393 6661 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 847 573 508 462 420 315 268 168 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6749 AflIII A'CryG_T Cuts at: 1203 3856 5845 6822 1203 Size: 2653 1989 977 1327 Fragments arranged by size: 2653 1989 1327 977 AgeI A'CCGG_T Cuts at: 5777 5777 Size: 6946 AhdI GACnn_n'nnGTC Cuts at: 5675 5675 Size: 6946 AleI CACnn'nnGTG Cuts at: 1166 2877 1166 Size: 1711 5235 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6946 AlwNI CAG_nnn'CTG Cuts at: 4689 6261 4689 Size: 1572 5374 ApaLI G'TGCA_C Cuts at: 3698 5003 6159 3698 Size: 1305 1156 4485 Fragments arranged by size: 4485 1305 1156 ApoI r'AATT_y Cuts at: 1493 2332 2344 2350 2700 2784 3184 3204 3456 Size: 839 12 6 350 84 400 20 252 Cuts at: 3456 3540 4044 4255 4481 4633 6742 1493 Size: 84 504 211 226 152 2109 1697 Fragments arranged by size: 2109 1697 839 504 400 350 252 226 211 152 84 84 20 12 6 AseI AT'TA_AT Cuts at: 160 1032 3258 5500 160 Size: 872 2226 2242 1606 Fragments arranged by size: 2242 2226 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6946 AvaI C'yCGr_G Cuts at: 1870 1943 4271 1870 Size: 73 2328 4545 Fragments arranged by size: 4545 2328 73 BamHI G'GATC_C Cuts at: 4276 4643 4276 Size: 367 6579 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4267 5722 618 Size: 325 217 627 2480 1455 1842 Fragments arranged by size: 2480 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2031 4265 1391 Size: 640 2234 4072 Fragments arranged by size: 4072 2234 640 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2234 2957 928 Size: 971 335 723 4917 Fragments arranged by size: 4917 971 723 335 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5137 5171 1903 Size: 34 3200 34 3678 Fragments arranged by size: 3678 3200 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4838 6048 4838 Size: 1210 5736 BclI T'GATC_A Cuts at: 1548 1548 Size: 6946 BfrBI ATG'CAT Cuts at: 3292 4132 3292 Size: 840 6106 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5557 136 Size: 107 122 71 631 605 3885 1525 Fragments arranged by size: 3885 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6941 6941 Size: 6946 BlpI GC'TnA_GC Cuts at: 4363 4363 Size: 6946 Bme1580I G_kGCm'C Cuts at: 1790 2764 3702 5007 6163 1790 Size: 974 938 1305 1156 2573 Fragments arranged by size: 2573 1305 1156 974 938 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6946 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3138 4062 4677 Size: 416 53 83 399 24 1721 924 615 Cuts at: 4677 5635 442 Size: 958 1753 Fragments arranged by size: 1753 1721 958 924 615 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6946 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2646 2814 3066 3486 3570 3654 3738 3822 Size: 1407 168 252 420 84 84 84 84 Cuts at: 3822 3990 4158 5606 1239 Size: 168 168 1448 2579 Fragments arranged by size: 2579 1448 1407 420 252 168 168 168 84 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2207 3509 4144 5071 5936 6234 2207 Size: 1302 635 927 865 298 2919 Fragments arranged by size: 2919 1302 927 865 635 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5609 882 Size: 617 4110 2219 Fragments arranged by size: 4110 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4243 4737 493 Size: 711 3039 494 2702 Fragments arranged by size: 3039 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2260 6940 2260 Size: 4680 2266 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4753 5135 5770 275 Size: 53 83 186 936 3220 382 635 1451 Fragments arranged by size: 3220 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5379 5777 6051 6198 6609 5379 Size: 398 274 147 411 5716 Fragments arranged by size: 5716 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6721 Fragments arranged by size: 6721 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6946 BseYI C'CCAG_C Cuts at: 6149 6645 6149 Size: 496 6450 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6373 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4396 5157 6185 1540 Size: 275 186 2395 761 1028 2301 Fragments arranged by size: 2395 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3702 5007 5092 6163 3702 Size: 1305 85 1071 4485 Fragments arranged by size: 4485 1305 1071 85 BsmI GAATG_Cn' Cuts at: 2053 2825 3167 3251 3294 3503 4457 4550 2053 Size: 772 342 84 43 209 954 93 4449 Fragments arranged by size: 4449 954 772 342 209 93 84 43 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2764 3702 4265 5007 5092 6163 Size: 399 241 733 938 563 742 85 1071 Cuts at: 6163 1391 Size: 2174 Fragments arranged by size: 2174 1071 938 742 733 563 399 241 85 BspEI T'CCGG_A Cuts at: 6609 6609 Size: 6946 BspHI T'CATG_A Cuts at: 4835 6565 4835 Size: 1730 5216 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2044 4301 844 Size: 1200 2257 3489 Fragments arranged by size: 3489 2257 1200 BsrBI CCG'CTC Cuts at: 1394 1823 4833 1394 Size: 429 3010 3507 Fragments arranged by size: 3507 3010 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2286 2510 2667 3409 3745 5441 5615 Size: 1183 1038 224 157 742 336 1696 174 Cuts at: 5615 65 Size: 1396 Fragments arranged by size: 1696 1396 1183 1038 742 336 224 174 157 BsrFI r'CCGG_y Cuts at: 1700 1810 5590 5777 1700 Size: 110 3780 187 2869 Fragments arranged by size: 3780 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2941 3277 3445 3613 4117 4201 96 Size: 2845 336 168 168 504 84 2841 Fragments arranged by size: 2845 2841 504 336 168 168 84 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6946 BssSI C'ACGA_G Cuts at: 5006 6018 5006 Size: 1012 5934 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6946 BstBI TT'CG_AA Cuts at: 2007 5750 2007 Size: 3743 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6946 BstYI r'GATC_y Cuts at: 1972 4276 4643 5028 5045 6497 6583 6595 6941 Size: 2304 367 385 17 1452 86 12 346 Cuts at: 6941 1972 Size: 1977 Fragments arranged by size: 2304 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 5437 Fragments arranged by size: 5437 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3163 487 Size: 683 56 1937 4270 Fragments arranged by size: 4270 1937 683 56 BtsI GCAGTG_nn' Cuts at: 4462 5256 5276 4462 Size: 794 20 6132 Fragments arranged by size: 6132 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6946 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2971 3006 3391 3426 3727 3762 529 Size: 35 2407 35 385 35 301 35 3713 Fragments arranged by size: 3713 2407 385 301 35 35 35 35 DraI TTT'AAA Cuts at: 2349 3817 4250 2349 Size: 1468 433 5045 Fragments arranged by size: 5045 1468 433 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5953 809 Size: 507 4637 1802 Fragments arranged by size: 4637 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4393 5282 6911 Size: 54 1425 50 275 18 2563 889 1629 Cuts at: 6911 8 Size: 43 Fragments arranged by size: 2563 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4393 1537 Size: 275 2581 4090 Fragments arranged by size: 4090 2581 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2292 2958 3378 4876 1353 Size: 939 666 420 1498 3423 Fragments arranged by size: 3423 1498 939 666 420 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5533 5907 6053 387 Size: 1064 138 3944 374 146 1280 Fragments arranged by size: 3944 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2409 2646 2814 2982 3066 3402 Size: 393 462 315 237 168 168 84 336 Cuts at: 3402 3486 3570 3654 3738 3822 3910 3990 4078 Size: 84 84 84 84 84 88 80 88 Cuts at: 4078 4158 4162 5009 5606 6393 6661 1239 Size: 80 4 847 597 787 268 1524 Fragments arranged by size: 1524 847 787 597 462 393 336 315 268 237 168 168 88 88 84 84 84 84 84 84 80 80 4 EcoNI CCTnn'n_nnAGG Cuts at: 3928 3928 Size: 6946 EcoO109I rG'GnC_Cy Cuts at: 3624 4062 3624 Size: 438 6508 EcoRI G'AATT_C Cuts at: 2700 2784 3204 3456 4044 4255 4633 2700 Size: 84 420 252 588 211 378 5013 Fragments arranged by size: 5013 588 420 378 252 211 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2439 2439 Size: 6946 FspI TGC'GCA Cuts at: 5452 5452 Size: 6946 HaeII r_GCGC'y Cuts at: 1678 6093 1678 Size: 4415 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2499 2531 5587 5619 5661 Size: 32 135 32 927 32 3056 32 42 Cuts at: 5661 5693 1373 Size: 32 2626 Fragments arranged by size: 3056 2626 927 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4290 4536 669 Size: 988 281 2352 246 3079 Fragments arranged by size: 3079 2352 988 281 246 HindIII A'AGCT_T Cuts at: 748 2829 748 Size: 2081 4865 HpaI GTT'AAC Cuts at: 4536 4536 Size: 6946 KpnI G_GTAC'C Cuts at: 4271 4271 Size: 6946 MfeI C'AATT_G Cuts at: 4545 4545 Size: 6946 MluI A'CGCG_T Cuts at: 6822 6822 Size: 6946 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6060 6244 1305 Size: 4755 184 2007 Fragments arranged by size: 4755 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5124 Fragments arranged by size: 5124 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2877 2977 3481 3512 3649 3817 4904 Size: 648 1711 100 504 31 137 168 1087 Cuts at: 4904 5263 5422 518 Size: 359 159 2042 Fragments arranged by size: 2042 1711 1087 648 504 359 168 159 137 100 31 MspA1I CmG'CkG Cuts at: 1606 4362 5039 6187 6432 1606 Size: 2756 677 1148 245 2120 Fragments arranged by size: 2756 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5437 Fragments arranged by size: 5437 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6946 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6946 NotI GC'GGCC_GC Cuts at: 4393 4393 Size: 6946 NsiI A_TGCA'T Cuts at: 3294 4134 3294 Size: 840 6106 NspI r_CATG'y Cuts at: 1132 1229 2487 3860 4304 5849 1132 Size: 97 1258 1373 444 1545 2229 Fragments arranged by size: 2229 1545 1373 1258 444 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6946 PciI A'CATG_T Cuts at: 3856 5845 3856 Size: 1989 4957 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6946 PmeI GTTT'AAAC Cuts at: 4250 4250 Size: 6946 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5015 5047 6528 6560 5015 Size: 32 1481 32 5401 Fragments arranged by size: 5401 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3624 3624 Size: 6946 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6946 PsiI TTA'TAA Cuts at: 3312 4516 3312 Size: 1204 5742 PstI C_TGCA'G Cuts at: 830 4298 830 Size: 3468 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6946 SalI G'TCGA_C Cuts at: 1936 4288 1936 Size: 2352 4594 SapI GCTCTTCn'nnn_ Cuts at: 2292 2292 Size: 6946 SbfI CC_TGCA'GG Cuts at: 4298 4298 Size: 6946 ScaI AGT'ACT Cuts at: 5194 6906 5194 Size: 1712 5234 SfcI C'TryA_G Cuts at: 826 1045 3085 4294 5429 6110 6301 826 Size: 219 2040 1209 1135 681 191 1471 Fragments arranged by size: 2040 1471 1209 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6946 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6946 SmaI CCC'GGG Cuts at: 1872 4273 1872 Size: 2401 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2222 3524 4123 5050 5951 6213 Size: 197 926 279 1302 599 927 901 262 Cuts at: 6213 820 Size: 1553 Fragments arranged by size: 1553 1302 927 926 901 599 279 262 197 SnaBI TAC'GTA Cuts at: 493 4243 4737 493 Size: 3750 494 2702 Fragments arranged by size: 3750 2702 494 SpeI A'CTAG_T Cuts at: 152 2237 152 Size: 2085 4861 SphI G_CATG'C Cuts at: 1132 4304 1132 Size: 3172 3774 SspI AAT'ATT Cuts at: 5 52 4870 5 Size: 47 4818 2081 Fragments arranged by size: 4818 2081 47 StuI AGG'CCT Cuts at: 1517 2495 2663 3335 1517 Size: 978 168 672 5128 Fragments arranged by size: 5128 978 672 168 StyI C'CwwG_G Cuts at: 513 1065 2022 4385 513 Size: 552 957 2363 3074 Fragments arranged by size: 3074 2363 957 552 SwaI ATTT'AAAT Cuts at: 2349 3817 2349 Size: 1468 5478 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4973 4990 5143 5328 5773 325 Size: 1255 3393 17 153 185 445 1498 Fragments arranged by size: 3393 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2480 2941 3277 Size: 274 80 33 51 1443 503 461 336 Cuts at: 3277 3317 3445 3485 3613 4117 4201 5192 6904 Size: 40 128 40 128 504 84 991 1712 Cuts at: 6904 96 Size: 138 Fragments arranged by size: 1712 1443 991 504 503 461 336 274 138 128 128 84 80 51 40 40 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6906 6938 6906 Size: 32 6914 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6946 XbaI T'CTAG_A Cuts at: 4282 4282 Size: 6946 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6946 XmaI C'CCGG_G Cuts at: 1870 4271 1870 Size: 2401 4545 XmnI GAAnn'nnTTC Cuts at: 2265 2301 2579 3094 3167 3251 3671 5075 2265 Size: 36 278 515 73 84 420 1404 4136 Fragments arranged by size: 4136 1404 515 420 278 84 73 36 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4754 276 Size: 53 83 186 936 3220 2468 Fragments arranged by size: 3220 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI XcmI