Restriction map for
pFN21ASDA1349
(Linear) (Six-base) MAPSORT of: KIAA1349.seq Check: 6866 from: 1 to: 2217
KIAA1349.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 386 959 1379 1887 2055 2139 2217
Size: 71 315 573 420 508 168 84 78
Fragments arranged by size:
573 508 420 315 168 84 78 71
AflIII A'CryG_T
Cuts at: 0 1833 2217
Size: 1833 384
AleI CACnn'nnGTG
Cuts at: 0 854 2217
Size: 854 1363
ApaLI G'TGCA_C
Cuts at: 0 1675 2217
Size: 1675 542
ApoI r'AATT_y
Cuts at: 0 309 321 327 677 761 1161 1181 1433
Size: 309 12 6 350 84 400 20 252
Cuts at: 1433 1517 2021 2217
Size: 84 504 196
Fragments arranged by size:
504 400 350 309 252 196 84 84
20 12 6
AseI AT'TA_AT
Cuts at: 0 1235 2217
Size: 1235 982
BanII G_rGCy'C
Cuts at: 0 8 2217
Size: 8 2209
BbsI GAAGACnn'nnnn_
Cuts at: 0 211 934 2217
Size: 211 723 1283
Fragments arranged by size:
1283 723 211
BfrBI ATG'CAT
Cuts at: 0 1269 2109 2217
Size: 1269 840 108
Fragments arranged by size:
1269 840 108
Bme1580I G_kGCm'C
Cuts at: 0 741 1679 2217
Size: 741 938 538
Fragments arranged by size:
938 741 538
BmrI ACTGGGnnnn_n'
Cuts at: 0 1115 2039 2217
Size: 1115 924 178
Fragments arranged by size:
1115 924 178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 791 1043 1463 1547 1631 1715 1799
Size: 623 168 252 420 84 84 84 84
Cuts at: 1799 1967 2135 2217
Size: 168 168 82
Fragments arranged by size:
623 420 252 168 168 168 84 84
84 84 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1486 2121 2217
Size: 184 1302 635 96
Fragments arranged by size:
1302 635 184 96
BsaBI GATnn'nnATC
Cuts at: 0 237 2217
Size: 237 1980
BsiHKAI G_wGCw'C
Cuts at: 0 1679 2217
Size: 1679 538
BsmI GAATG_Cn'
Cuts at: 0 30 802 1144 1228 1271 1480 2217
Size: 30 772 342 84 43 209 737
Fragments arranged by size:
772 737 342 209 84 43 30
Bsp1286I G_dGCh'C
Cuts at: 0 8 741 1679 2217
Size: 8 733 938 538
Fragments arranged by size:
938 733 538 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 21 2217
Size: 21 2196
BsrDI GCAATG_nn'
Cuts at: 0 263 487 644 1386 1722 2217
Size: 263 224 157 742 336 495
Fragments arranged by size:
742 495 336 263 224 157
BsrGI T'GTAC_A
Cuts at: 0 918 1254 1422 1590 2094 2178 2217
Size: 918 336 168 168 504 84 39
Fragments arranged by size:
918 504 336 168 168 84 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1140 2217
Size: 1140 1077
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 948 983 1368 1403 1704 1739 2217
Size: 948 35 385 35 301 35 478
Fragments arranged by size:
948 478 385 301 35 35 35
DraI TTT'AAA
Cuts at: 0 326 1794 2217
Size: 326 1468 423
Fragments arranged by size:
1468 423 326
EarI CTCTTCn'nnn_
Cuts at: 0 269 935 1355 2217
Size: 269 666 420 862
Fragments arranged by size:
862 666 420 269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 386 623 791 959 1043 1379 1463
Size: 71 315 237 168 168 84 336 84
Cuts at: 1463 1547 1631 1715 1799 1887 1967 2055 2135
Size: 84 84 84 84 88 80 88 80
Cuts at: 2135 2139 2217
Size: 4 78
Fragments arranged by size:
336 315 237 168 168 88 88 84
84 84 84 84 84 80 80 78
71 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1905 2217
Size: 1905 312
EcoO109I rG'GnC_Cy
Cuts at: 0 1601 2039 2217
Size: 1601 438 178
Fragments arranged by size:
1601 438 178
EcoRI G'AATT_C
Cuts at: 0 677 761 1181 1433 2021 2217
Size: 677 84 420 252 588 196
Fragments arranged by size:
677 588 420 252 196 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 416 2217
Size: 416 1801
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 476 508 2217
Size: 476 32 1709
Fragments arranged by size:
1709 476 32
HindIII A'AGCT_T
Cuts at: 0 806 2217
Size: 806 1411
MslI CAynn'nnrTG
Cuts at: 0 854 954 1458 1489 1626 1794 2217
Size: 854 100 504 31 137 168 423
Fragments arranged by size:
854 504 423 168 137 100 31
NsiI A_TGCA'T
Cuts at: 0 1271 2111 2217
Size: 1271 840 106
Fragments arranged by size:
1271 840 106
NspI r_CATG'y
Cuts at: 0 464 1837 2217
Size: 464 1373 380
Fragments arranged by size:
1373 464 380
PciI A'CATG_T
Cuts at: 0 1833 2217
Size: 1833 384
PpuMI rG'GwC_Cy
Cuts at: 0 1601 2217
Size: 1601 616
PsiI TTA'TAA
Cuts at: 0 1289 2217
Size: 1289 928
SapI GCTCTTCn'nnn_
Cuts at: 0 269 2217
Size: 269 1948
SfcI C'TryA_G
Cuts at: 0 1062 2217
Size: 1062 1155
SmlI C'TyrA_G
Cuts at: 0 199 1501 2100 2217
Size: 199 1302 599 117
Fragments arranged by size:
1302 599 199 117
SpeI A'CTAG_T
Cuts at: 0 214 2217
Size: 214 2003
StuI AGG'CCT
Cuts at: 0 472 640 1312 2217
Size: 472 168 672 905
Fragments arranged by size:
905 672 472 168
SwaI ATTT'AAAT
Cuts at: 0 326 1794 2217
Size: 326 1468 423
Fragments arranged by size:
1468 423 326
TatI w'GTAC_w
Cuts at: 0 457 918 1254 1294 1422 1462 1590 2094
Size: 457 461 336 40 128 40 128 504
Cuts at: 2094 2178 2217
Size: 84 39
Fragments arranged by size:
504 461 457 336 128 128 84 40
40 39
XmnI GAAnn'nnTTC
Cuts at: 0 242 278 556 1071 1144 1228 1648 2217
Size: 242 36 278 515 73 84 420 569
Fragments arranged by size:
569 515 420 278 242 84 73 36
Enzymes that do cut:
AcuI AflIII AleI ApaLI ApoI AseI BanII BbsI
BfrBI Bme1580I BmrI BpmI BpuEI BsaBI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BsrGI BtgZI CspCI DraI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HindIII MslI
NsiI NspI PciI PpuMI PsiI SapI SfcI SmlI
SpeI StuI SwaI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI BmgBI BmtI BplI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtsI ClaI DraIII DrdI EaeI EagI EciI
EcoICRI EcoRV FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SspI StyI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI ZraI