(Circular) (Six-base) MAPSORT of: pFN21ASDA1345.seq Check: 5551 from: 1 to: 9265 pFN21ASDA1345 9265 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2575 2575 Size: 9265 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7075 278 Size: 53 83 186 936 5539 2468 Fragments arranged by size: 5539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5646 6489 6608 1937 Size: 3709 843 119 4594 Fragments arranged by size: 4594 3709 843 119 Acc65I G'GTAC_C Cuts at: 6586 6586 Size: 9265 AclI AA'CG_TT Cuts at: 1563 7392 7765 1563 Size: 5829 373 3063 Fragments arranged by size: 5829 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4037 4173 5247 6159 6426 7328 8712 8980 Size: 2405 136 1074 912 267 902 1384 268 Cuts at: 8980 1632 Size: 1917 Fragments arranged by size: 2405 1917 1384 1074 912 902 268 267 136 AfeI AGC'GCT Cuts at: 3914 6158 3914 Size: 2244 7021 AflII C'TTAA_G Cuts at: 820 1017 3392 820 Size: 197 2375 6693 Fragments arranged by size: 6693 2375 197 AflIII A'CryG_T Cuts at: 1203 5600 8164 9141 1203 Size: 4397 2564 977 1327 Fragments arranged by size: 4397 2564 1327 977 AgeI A'CCGG_T Cuts at: 8096 8096 Size: 9265 AhdI GACnn_n'nnGTC Cuts at: 3966 7994 3966 Size: 4028 5237 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9265 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9265 AlwNI CAG_nnn'CTG Cuts at: 7008 8580 7008 Size: 1572 7693 ApaLI G'TGCA_C Cuts at: 7322 8478 7322 Size: 1156 8109 ApoI r'AATT_y Cuts at: 1493 2165 2300 3082 3218 3336 3353 3528 4643 Size: 672 135 782 136 118 17 175 1115 Cuts at: 4643 5060 6055 6473 6574 6800 6952 9061 1493 Size: 417 995 418 101 226 152 2109 1697 Fragments arranged by size: 2109 1697 1115 995 782 672 418 417 226 175 152 136 135 118 101 17 AseI AT'TA_AT Cuts at: 160 1032 7819 160 Size: 872 6787 1606 Fragments arranged by size: 6787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9265 AvaI C'yCGr_G Cuts at: 1870 1943 3710 3889 6590 1870 Size: 73 1767 179 2701 4545 Fragments arranged by size: 4545 2701 1767 179 73 BamHI G'GATC_C Cuts at: 2922 6595 6962 2922 Size: 3673 367 5225 Fragments arranged by size: 5225 3673 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4636 6586 8041 618 Size: 325 217 627 2849 1950 1455 1842 Fragments arranged by size: 2849 1950 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2079 3747 3819 4147 5278 5637 6584 1391 Size: 688 1668 72 328 1131 359 947 4072 Fragments arranged by size: 4072 1668 1131 947 688 359 328 72 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5073 928 Size: 971 3174 5120 Fragments arranged by size: 5120 3174 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5190 5224 7456 7490 1903 Size: 34 3253 34 2232 34 3678 Fragments arranged by size: 3678 3253 2232 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3717 4308 7157 8367 3717 Size: 591 2849 1210 4615 Fragments arranged by size: 4615 2849 1210 591 BclI T'GATC_A Cuts at: 1548 3057 5761 1548 Size: 1509 2704 5052 Fragments arranged by size: 5052 2704 1509 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3167 4436 7876 Size: 107 122 71 631 605 1495 1269 3440 Cuts at: 7876 136 Size: 1525 Fragments arranged by size: 3440 1525 1495 1269 631 605 122 107 71 BglII A'GATC_T Cuts at: 3062 9260 3062 Size: 6198 3067 BlpI GC'TnA_GC Cuts at: 3836 6180 6682 3836 Size: 2344 502 6419 Fragments arranged by size: 6419 2344 502 Bme1580I G_kGCm'C Cuts at: 1790 3875 4221 7326 8482 1790 Size: 2085 346 3105 1156 2573 Fragments arranged by size: 3105 2573 2085 1156 346 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2271 2736 3166 Size: 416 53 83 399 24 854 465 430 Cuts at: 3166 6996 7954 442 Size: 3830 958 1753 Fragments arranged by size: 3830 1753 958 854 465 430 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4097 1056 Size: 3041 6224 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2145 2747 2145 Size: 602 8663 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3087 3646 3723 4565 4775 5770 6250 6462 Size: 1848 559 77 842 210 995 480 212 Cuts at: 6462 7925 1239 Size: 1463 2579 Fragments arranged by size: 2579 1848 1463 995 842 559 480 212 210 77 Bpu10I CC'TnA_GC Cuts at: 4901 4901 Size: 9265 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2350 7390 8255 8553 2350 Size: 5040 865 298 3062 Fragments arranged by size: 5040 3062 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3063 3892 7928 882 Size: 617 1564 829 4036 2219 Fragments arranged by size: 4036 2219 1564 829 617 BsaAI yAC'GTr Cuts at: 493 1204 2653 5124 6562 7056 493 Size: 711 1449 2471 1438 494 2702 Fragments arranged by size: 2702 2471 1449 1438 711 494 BsaBI GATnn'nnATC Cuts at: 6037 9259 6037 Size: 3222 6043 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7072 7454 8089 275 Size: 53 83 186 936 5539 382 635 1451 Fragments arranged by size: 5539 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2106 7698 8096 8370 8517 8928 2106 Size: 5592 398 274 147 411 2443 Fragments arranged by size: 5592 2443 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3442 3472 4939 4969 6306 Size: 30 165 30 2039 30 1467 30 1337 Cuts at: 6306 6336 1178 Size: 30 4107 Fragments arranged by size: 4107 2039 1467 1337 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 2241 2552 2555 2592 3584 3728 3817 Size: 901 154 311 3 37 992 144 89 Cuts at: 3817 5616 1186 Size: 1799 4835 Fragments arranged by size: 4835 1799 992 901 311 154 144 89 37 3 BseYI C'CCAG_C Cuts at: 3731 5422 6358 8468 8964 3731 Size: 1691 936 2110 496 4032 Fragments arranged by size: 4032 2110 1691 936 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2087 2373 2553 1163 Size: 573 351 286 180 7875 Fragments arranged by size: 7875 573 351 286 180 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6715 7476 8504 1540 Size: 275 186 4714 761 1028 2301 Fragments arranged by size: 4714 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3564 5637 5756 7326 7411 8482 3564 Size: 2073 119 1570 85 1071 4347 Fragments arranged by size: 4347 2073 1570 1071 119 85 BsmI GAATG_Cn' Cuts at: 6776 6869 6776 Size: 93 9172 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 3564 3747 3819 3875 4147 4221 Size: 399 289 1485 183 72 56 272 74 Cuts at: 4221 5278 5637 5756 6584 7326 7411 8482 1391 Size: 1057 359 119 828 742 85 1071 2174 Fragments arranged by size: 2174 1485 1071 1057 828 742 399 359 289 272 183 119 85 74 72 56 BspEI T'CCGG_A Cuts at: 2106 8928 2106 Size: 6822 2443 BspHI T'CATG_A Cuts at: 7154 8884 7154 Size: 1730 7535 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2575 2689 4786 6620 844 Size: 1731 114 2097 1834 3489 Fragments arranged by size: 3489 2097 1834 1731 114 BsrBI CCG'CTC Cuts at: 1394 1823 2522 3881 7152 1394 Size: 429 699 1359 3271 3507 Fragments arranged by size: 3507 3271 1359 699 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2228 3386 5869 7760 7934 65 Size: 1183 980 1158 2483 1891 174 1396 Fragments arranged by size: 2483 1891 1396 1183 1158 980 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5047 7909 8096 1700 Size: 110 3237 2862 187 2869 Fragments arranged by size: 3237 2869 2862 187 110 BsrGI T'GTAC_A Cuts at: 96 2785 96 Size: 2689 6576 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9265 BssSI C'ACGA_G Cuts at: 4196 7325 8337 4196 Size: 3129 1012 5124 Fragments arranged by size: 5124 3129 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2225 794 Size: 1431 7834 BstBI TT'CG_AA Cuts at: 2007 8069 2007 Size: 6062 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9265 BstXI CCAn_nnnn'nTGG Cuts at: 2800 2800 Size: 9265 BstYI r'GATC_y Cuts at: 1972 2922 3062 4595 6528 6595 6962 7347 7364 Size: 950 140 1533 1933 67 367 385 17 Cuts at: 7364 8816 8902 8914 9260 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1933 1533 1452 950 385 367 346 140 86 67 17 12 BstZ17I GTA'TAC Cuts at: 6490 6490 Size: 9265 Bsu36I CC'TnA_GG Cuts at: 4267 5628 5874 4267 Size: 1361 246 7658 Fragments arranged by size: 7658 1361 246 BtgI C'CryG_G Cuts at: 513 1065 2022 2117 513 Size: 552 957 95 7661 Fragments arranged by size: 7661 957 552 95 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8526 Fragments arranged by size: 8526 683 56 BtsI GCAGTG_nn' Cuts at: 3765 4934 5058 5944 6781 7575 7595 3765 Size: 1169 124 886 837 794 20 5435 Fragments arranged by size: 5435 1169 886 837 794 124 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9265 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9230 DraI TTT'AAA Cuts at: 5619 6569 5619 Size: 950 8315 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4080 8272 809 Size: 507 2764 4192 1802 Fragments arranged by size: 4192 2764 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3971 4034 4428 Size: 54 1425 50 275 18 2141 63 394 Cuts at: 4428 4940 5158 6712 7601 9230 8 Size: 512 218 1554 889 1629 43 Fragments arranged by size: 2141 1629 1554 1425 889 512 394 275 218 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6712 1537 Size: 275 4900 4090 Fragments arranged by size: 4900 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2436 2738 3552 6135 6393 7195 1353 Size: 1083 302 814 2583 258 802 3423 Fragments arranged by size: 3423 2583 1083 814 802 302 258 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7852 8226 8372 387 Size: 1064 138 6263 374 146 1280 Fragments arranged by size: 6263 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5635 5635 Size: 9265 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3087 3646 3723 4037 4173 4565 4775 Size: 393 1455 559 77 314 136 392 210 Cuts at: 4775 5247 5770 6159 6250 6426 6462 7328 7925 Size: 472 523 389 91 176 36 866 597 Cuts at: 7925 8712 8980 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1455 866 787 597 559 523 472 393 392 389 314 268 210 176 136 91 77 36 EcoNI CCTnn'n_nnAGG Cuts at: 2571 4793 5159 2571 Size: 2222 366 6677 Fragments arranged by size: 6677 2222 366 EcoO109I rG'GnC_Cy Cuts at: 2887 3718 6002 2887 Size: 831 2284 6150 Fragments arranged by size: 6150 2284 831 EcoRI G'AATT_C Cuts at: 4643 6574 6952 4643 Size: 1931 378 6956 Fragments arranged by size: 6956 1931 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2624 2624 Size: 9265 FspI TGC'GCA Cuts at: 7771 7771 Size: 9265 HaeII r_GCGC'y Cuts at: 1678 3916 6160 8412 1678 Size: 2238 2244 2252 2531 Fragments arranged by size: 2531 2252 2244 2238 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2914 2946 3314 3346 3410 Size: 32 135 32 1342 32 368 32 64 Cuts at: 3410 3442 3870 3902 3952 3984 4370 4402 4939 Size: 32 428 32 50 32 386 32 537 Cuts at: 4939 4971 5708 5740 7906 7938 7980 8012 1373 Size: 32 737 32 2166 32 42 32 2626 Fragments arranged by size: 2626 2166 1342 737 537 428 386 368 135 64 50 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2350 4030 6609 6855 669 Size: 988 281 412 1680 2579 246 3079 Fragments arranged by size: 3079 2579 1680 988 412 281 246 HindIII A'AGCT_T Cuts at: 748 2771 3240 748 Size: 2023 469 6773 Fragments arranged by size: 6773 2023 469 HpaI GTT'AAC Cuts at: 6855 6855 Size: 9265 KpnI G_GTAC'C Cuts at: 6590 6590 Size: 9265 MfeI C'AATT_G Cuts at: 6864 6864 Size: 9265 MluI A'CGCG_T Cuts at: 9141 9141 Size: 9265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3112 4051 4186 4937 5906 8379 8563 1305 Size: 1807 939 135 751 969 2473 184 2007 Fragments arranged by size: 2473 2007 1807 969 939 751 184 135 MscI TGG'CCA Cuts at: 10 64 1489 1832 4430 10 Size: 54 1425 343 2598 4845 Fragments arranged by size: 4845 2598 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3684 5036 5949 7223 7582 7741 518 Size: 648 2518 1352 913 1274 359 159 2042 Fragments arranged by size: 2518 2042 1352 1274 913 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4252 4552 6173 6179 6358 6681 7358 8506 Size: 2646 300 1621 6 179 323 677 1148 Cuts at: 8506 8751 1606 Size: 245 2120 Fragments arranged by size: 2646 2120 1621 1148 677 323 300 245 179 6 NaeI GCC'GGC Cuts at: 5049 5049 Size: 9265 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 7756 Fragments arranged by size: 7756 957 552 NdeI CA'TA_TG Cuts at: 387 3517 387 Size: 3130 6135 NgoMIV G'CCGG_C Cuts at: 5047 5047 Size: 9265 NheI G'CTAG_C Cuts at: 1052 4093 1052 Size: 3041 6224 NotI GC'GGCC_GC Cuts at: 6712 6712 Size: 9265 NspI r_CATG'y Cuts at: 1132 1229 3056 3152 4184 5108 5804 6397 6623 Size: 97 1827 96 1032 924 696 593 226 Cuts at: 6623 8168 1132 Size: 1545 2229 Fragments arranged by size: 2229 1827 1545 1032 924 696 593 226 97 96 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9265 PciI A'CATG_T Cuts at: 8164 8164 Size: 9265 PflMI CCAn_nnn'nTGG Cuts at: 1108 6081 6116 1108 Size: 4973 35 4257 Fragments arranged by size: 4973 4257 35 PfoI T'CCnGG_A Cuts at: 2799 3076 3828 3956 4701 2799 Size: 277 752 128 745 7363 Fragments arranged by size: 7363 752 745 277 128 PmeI GTTT'AAAC Cuts at: 6569 6569 Size: 9265 PmlI CAC'GTG Cuts at: 2653 2653 Size: 9265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7334 7366 8847 8879 7334 Size: 32 1481 32 7720 Fragments arranged by size: 7720 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2887 3718 6002 2887 Size: 831 2284 6150 Fragments arranged by size: 6150 2284 831 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9265 PsiI TTA'TAA Cuts at: 4656 6835 4656 Size: 2179 7086 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3021 3053 5790 5822 3021 Size: 32 2737 32 6464 Fragments arranged by size: 6464 2737 32 32 PstI C_TGCA'G Cuts at: 830 2238 2440 2686 2715 5310 5674 5939 6178 Size: 1408 202 246 29 2595 364 265 239 Cuts at: 6178 6617 830 Size: 439 3478 Fragments arranged by size: 3478 2595 1408 439 364 265 246 239 202 29 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9265 PvuII CAG'CTG Cuts at: 4252 4552 6173 6179 6358 4252 Size: 300 1621 6 179 7159 Fragments arranged by size: 7159 1621 300 179 6 SacI G_AGCT'C Cuts at: 5637 5637 Size: 9265 SalI G'TCGA_C Cuts at: 1936 6607 1936 Size: 4671 4594 SapI GCTCTTCn'nnn_ Cuts at: 2738 3552 6135 2738 Size: 814 2583 5868 Fragments arranged by size: 5868 2583 814 SbfI CC_TGCA'GG Cuts at: 6617 6617 Size: 9265 ScaI AGT'ACT Cuts at: 2973 5352 5808 6439 7513 9225 2973 Size: 2379 456 631 1074 1712 3013 Fragments arranged by size: 3013 2379 1712 1074 631 456 SfcI C'TryA_G Cuts at: 826 1045 2234 2436 2682 2711 5306 5660 5670 Size: 219 1189 202 246 29 2595 354 10 Cuts at: 5670 5935 6174 6367 6613 7748 8429 8620 826 Size: 265 239 193 246 1135 681 191 1471 Fragments arranged by size: 2595 1471 1189 1135 681 354 265 246 246 239 219 202 193 191 29 10 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9265 SmaI CCC'GGG Cuts at: 1872 6592 1872 Size: 4720 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2365 3392 3889 7369 8270 8532 Size: 197 926 422 1027 497 3480 901 262 Cuts at: 8532 820 Size: 1553 Fragments arranged by size: 3480 1553 1027 926 901 497 422 262 197 SnaBI TAC'GTA Cuts at: 493 6562 7056 493 Size: 6069 494 2702 Fragments arranged by size: 6069 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9265 SphI G_CATG'C Cuts at: 1132 5108 6623 1132 Size: 3976 1515 3774 Fragments arranged by size: 3976 3774 1515 SspI AAT'ATT Cuts at: 5 52 4841 6013 6025 6454 7189 5 Size: 47 4789 1172 12 429 735 2081 Fragments arranged by size: 4789 2081 1172 735 429 47 12 StuI AGG'CCT Cuts at: 1517 2447 2814 3623 3808 5846 1517 Size: 930 367 809 185 2038 4936 Fragments arranged by size: 4936 2038 930 809 367 185 StyI C'CwwG_G Cuts at: 513 1065 2022 2044 3809 4746 4943 6704 513 Size: 552 957 22 1765 937 197 1761 3074 Fragments arranged by size: 3074 1765 1761 957 937 552 197 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3874 4692 4778 6513 7292 7309 7462 Size: 1255 2294 818 86 1735 779 17 153 Cuts at: 7462 7647 8092 325 Size: 185 445 1498 Fragments arranged by size: 2294 1735 1498 1255 818 779 445 185 153 86 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2785 2971 3921 Size: 274 80 33 51 1443 808 186 950 Cuts at: 3921 5350 5806 6437 6445 7511 9223 96 Size: 1429 456 631 8 1066 1712 138 Fragments arranged by size: 1712 1443 1429 1066 950 808 631 456 274 186 138 80 51 33 8 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2412 2444 3112 3144 4200 4232 5080 5112 9225 Size: 32 668 32 1056 32 848 32 4113 Cuts at: 9225 9257 2412 Size: 32 2420 Fragments arranged by size: 4113 2420 1056 848 668 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5720 1316 Size: 4404 4861 XbaI T'CTAG_A Cuts at: 6601 6601 Size: 9265 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2420 4202 5241 2420 Size: 1782 1039 6444 Fragments arranged by size: 6444 1782 1039 XhoI C'TCGA_G Cuts at: 1943 3889 1943 Size: 1946 7319 XmaI C'CCGG_G Cuts at: 1870 6590 1870 Size: 4720 4545 XmnI GAAnn'nnTTC Cuts at: 4104 4875 6089 7394 4104 Size: 771 1214 1305 5975 Fragments arranged by size: 5975 1305 1214 771 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7073 276 Size: 53 83 186 936 5539 2468 Fragments arranged by size: 5539 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI