Restriction map for
pFN21ASDA1344
(Linear) (Six-base) MAPSORT of: KIAA1344.seq Check: 4516 from: 1 to: 2475
KIAA1344.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 779 788 1703 1867 2102 2179 2475
Size: 424 355 9 915 164 235 77 296
Fragments arranged by size:
915 424 355 296 235 164 77 9
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 343 2475
Size: 343 2132
AlwNI CAG_nnn'CTG
Cuts at: 0 1135 2475
Size: 1135 1340
ApoI r'AATT_y
Cuts at: 0 227 337 997 1387 1468 1673 2475
Size: 227 110 660 390 81 205 802
Fragments arranged by size:
802 660 390 227 205 110 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 440 880 957 1085 1717 2475
Size: 440 440 77 128 632 758
Fragments arranged by size:
758 632 440 440 128 77
BciVI GTATCCnnnnn_n'
Cuts at: 0 1272 2475
Size: 1272 1203
BglII A'GATC_T
Cuts at: 0 1458 2475
Size: 1458 1017
BmgBI CAC'GTC
Cuts at: 0 2443 2475
Size: 2443 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 814 1420 1862 2475
Size: 814 606 442 613
Fragments arranged by size:
814 613 606 442
BmtI G_CTAG'C
Cuts at: 0 1787 2475
Size: 1787 688
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 928 2475
Size: 928 1547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1997 2475
Size: 1997 478
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 932 2192 2475
Size: 932 1260 283
Fragments arranged by size:
1260 932 283
BsaHI Gr'CG_yC
Cuts at: 0 885 2475
Size: 885 1590
BsaWI w'CCGG_w
Cuts at: 0 1842 2475
Size: 1842 633
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 977 1007 2475
Size: 977 30 1468
Fragments arranged by size:
1468 977 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2213 2475
Size: 2213 262
BseYI C'CCAG_C
Cuts at: 0 668 2475
Size: 668 1807
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1367 2338 2475
Size: 1367 971 137
Fragments arranged by size:
1367 971 137
BsiHKAI G_wGCw'C
Cuts at: 0 518 2475
Size: 518 1957
BsmI GAATG_Cn'
Cuts at: 0 372 2088 2475
Size: 372 1716 387
Fragments arranged by size:
1716 387 372
Bsp1286I G_dGCh'C
Cuts at: 0 518 2475
Size: 518 1957
BspEI T'CCGG_A
Cuts at: 0 1842 2475
Size: 1842 633
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2211 2475
Size: 2211 264
BsrDI GCAATG_nn'
Cuts at: 0 115 679 2475
Size: 115 564 1796
Fragments arranged by size:
1796 564 115
BsrFI r'CCGG_y
Cuts at: 0 535 2475
Size: 535 1940
BsrGI T'GTAC_A
Cuts at: 0 1406 2475
Size: 1406 1069
BstYI r'GATC_y
Cuts at: 0 32 771 1458 2475
Size: 32 739 687 1017
Fragments arranged by size:
1017 739 687 32
BtgI C'CryG_G
Cuts at: 0 1265 2475
Size: 1265 1210
ClaI AT'CG_AT
Cuts at: 0 1513 2475
Size: 1513 962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 564 599 2475
Size: 564 35 1876
Fragments arranged by size:
1876 564 35
DraI TTT'AAA
Cuts at: 0 2418 2475
Size: 2418 57
DrdI GACnn_nn'nnGTC
Cuts at: 0 1598 2475
Size: 1598 877
EaeI y'GGCC_r
Cuts at: 0 2097 2475
Size: 2097 378
EarI CTCTTCn'nnn_
Cuts at: 0 401 974 1147 1243 2072 2231 2475
Size: 401 573 173 96 829 159 244
Fragments arranged by size:
829 573 401 244 173 159 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 779 788 1703 1867 1997 2102 2179
Size: 424 355 9 915 164 130 105 77
Cuts at: 2179 2475
Size: 296
Fragments arranged by size:
915 424 355 296 164 130 105 77
9
EcoRI G'AATT_C
Cuts at: 0 337 2475
Size: 337 2138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 944 2161 2475
Size: 944 1217 314
Fragments arranged by size:
1217 944 314
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 17 49 1479 1511 1649 1681 2364 2396
Size: 17 32 1430 32 138 32 683 32
Cuts at: 2396 2475
Size: 79
Fragments arranged by size:
1430 683 138 79 32 32 32 32
17
HincII GTy'rAC
Cuts at: 0 755 1706 2475
Size: 755 951 769
Fragments arranged by size:
951 769 755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 825 1627 2475
Size: 825 802 848
Fragments arranged by size:
848 825 802
MscI TGG'CCA
Cuts at: 0 2099 2475
Size: 2099 376
MslI CAynn'nnrTG
Cuts at: 0 1402 2093 2475
Size: 1402 691 382
Fragments arranged by size:
1402 691 382
NcoI C'CATG_G
Cuts at: 0 1265 2475
Size: 1265 1210
NdeI CA'TA_TG
Cuts at: 0 489 2475
Size: 489 1986
NheI G'CTAG_C
Cuts at: 0 1783 2475
Size: 1783 692
NspI r_CATG'y
Cuts at: 0 66 1795 2475
Size: 66 1729 680
Fragments arranged by size:
1729 680 66
PstI C_TGCA'G
Cuts at: 0 218 870 2475
Size: 218 652 1605
Fragments arranged by size:
1605 652 218
SapI GCTCTTCn'nnn_
Cuts at: 0 401 2475
Size: 401 2074
ScaI AGT'ACT
Cuts at: 0 1235 2475
Size: 1235 1240
SfcI C'TryA_G
Cuts at: 0 214 866 2475
Size: 214 652 1609
Fragments arranged by size:
1609 652 214
SmlI C'TyrA_G
Cuts at: 0 947 2207 2475
Size: 947 1260 268
Fragments arranged by size:
1260 947 268
SpeI A'CTAG_T
Cuts at: 0 651 2475
Size: 651 1824
SspI AAT'ATT
Cuts at: 0 106 477 1534 1946 2475
Size: 106 371 1057 412 529
Fragments arranged by size:
1057 529 412 371 106
StyI C'CwwG_G
Cuts at: 0 239 1265 2475
Size: 239 1026 1210
Fragments arranged by size:
1210 1026 239
TatI w'GTAC_w
Cuts at: 0 112 1233 1366 1406 2475
Size: 112 1121 133 40 1069
Fragments arranged by size:
1121 1069 133 112 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 977 1009 1908 1940 2475
Size: 977 32 899 32 535
Fragments arranged by size:
977 899 535 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 437 1677 1838 1862 2475
Size: 437 1240 161 24 613
Fragments arranged by size:
1240 613 437 161 24
Enzymes that do cut:
AcuI AlfI AlwNI ApoI BbsI BciVI BglII BmgBI
BmrI BmtI BplI BpmI BpuEI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrDI BsrFI BsrGI BstYI BtgI ClaI CspCI DraI
DrdI EaeI EarI Eco57MI EcoRI FalI Hin4I HincII
MmeI MscI MslI NcoI NdeI NheI NspI PstI
SapI ScaI SfcI SmlI SpeI SspI StyI TatI
TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BclI BfrBI BglI BlpI Bme1580I Bpu10I
BsaI BsaAI BsaBI BsiEI BsiWI BsmBI BspHI BsrBI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI DraIII EagI EciI EcoICRI EcoNI EcoO109I
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NgoMIV NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI
SrfI StuI SwaI TaqII Tth111I XbaI XcmI XhoI
XmaI ZraI