Restriction map for
pFN21ASDA1341
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BalI BbeI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspEI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII CfoI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I FbaI FseI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HpaI KasI KpnI MluI MluNI
MroI MscI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SphI Sse8387I SspBI StuI StyI
SwaI XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 7 GACGCC G[GA]^CG[CT]C
AflIII 588 ACACGT A^C[GA][CT]GT
Asp700I 853 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 391 GAGCTC G[AT]GC[AT]^C
AspI 1010 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 28 CCCGGG C^[CT]CG[GA]G
BamHI 525 GGATCC G^GATCC
BanI 24 GGTGCC G^G[CT][GA]CC
BanII 391 GAGCTC G[GA]GC[CT]^C
BbrPI 983 CACGTG CAC^GTG
BglI 10 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 351 AGATCT A^GATCT
BsaHI 7 GACGCC G[GA]^CG[CT]C
BsaWI 1433 ACCGGA [AT]^CCGG[AT]
BsiHKAI 391 GAGCTC G[AT]GC[AT]^C
BsoBI 28 CCCGGG C^[CT]CG[GA]G
BspDI 1495 ATCGAT AT^CGAT
BspHI 939 TCATGA T^CATGA
BspMI 56 ACCTGC ACCTGC(4/8)
BsrFI 682 GCCGGT [GA]^CCGG[CT]
Cfr10I 682 GCCGGT [GA]^CCGG[CT]
ClaI 1495 ATCGAT AT^CGAT
DdeI 496 CTTAG C^T[ACGT]AG
EaeI 1194 TGGCCG [CT]^GGCC[GA]
EcoT22I 907 ATGCAT ATGCA^T
HgaI 7 GACGC GACGC(5/10)
Hin1I 7 GACGCC G[GA]^CG[CT]C
MfeI 848 CAATTG C^AATTG
MunI 848 CAATTG C^AATTG
NdeI 323 CATATG CA^TATG
NsiI 907 ATGCAT ATGCA^T
NspI 909 GCATGT [GA]CATG^[CT]
PflFI 1010 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1105 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1708 GACTC GAGTC(4/5)
PmaCI 983 CACGTG CAC^GTG
PmlI 983 CACGTG CAC^GTG
PvuII 1721 CAGCTG CAG^CTG
RcaI 939 TCATGA T^CATGA
SacI 391 GAGCTC GAGCT^C
SexAI 1464 ACCAGGT A^CC[AT]GGT
SmaI 28 CCCGGG CCC^GGG
SpeI 1228 ACTAGT A^CTAGT
SspI 514 AATATT AAT^ATT
Tth111I 1010 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1105 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 28 CCCGGG C^CCGGG
XmaI 28 CCCGGG C^CCGGG
XmnI 853 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 686 GTAGAC GT^[AC][GT]AC
1298 GTAGAC
AccII 86 CGCG CG^CG
105 CGCG
AfaI 948 GTAC GT^AC
1212 GTAC
BbsI 503 GAAGAC GAAGAC(2/6)
796 GAAGAC
BfaI 472 CTAG C^TAG
1229 CTAG
BpmI 272 CTGGAG CTGGAG(16/14)
1409 CTGGAG
BpuAI 503 GAAGAC GAAGAC(2/6)
796 GAAGAC
BsaAI 385 TACGTG [CT]AC^GT[GA]
983 CACGTG
BsaBI 1498 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1511 GATTTTTATC
BseRI 1133 GAGGAG GAGGAG(10/8)
1187 GAGGAG
BstUI 86 CGCG CG^CG
105 CGCG
EarI 184 GAAGAG CTCTTC(1/4)
943 GAAGAG
EcoRI 261 GAATTC G^AATTC
439 GAATTC
HphI 373 GGTGA GGTGA(8/7)
1258 GGTGA
Ksp632I 184 GAAGAG CTCTTC(1/4)
943 GAAGAG
KspI 85 CCGCGG CCGC^GG
104 CCGCGG
MaeI 472 CTAG C^TAG
1229 CTAG
MamI 1498 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1511 GATTTTTATC
MslI 999 CATTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1494 CATCGATATG
MvnI 86 CGCG CG^CG
105 CGCG
PstI 58 CTGCAG CTGCA^G
1734 CTGCAG
RsaI 948 GTAC GT^AC
1212 GTAC
SacII 85 CCGCGG CCGC^GG
104 CCGCGG
SfaNI 403 GATGC GCATC(5/9)
1493 GCATC
Tsp45I 832 GTCAC ^GT[GC]AC
1405 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BbvI BcnI BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DpnII DsaI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfcI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII