(Circular) (Six-base) MAPSORT of: pFN21ASDA1341.seq Check: 5539 from: 1 to: 6529 pFN21ASDA1341 6529 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2088 2088 Size: 6529 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4339 278 Size: 53 83 186 936 2803 2468 Fragments arranged by size: 2803 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2710 3322 3872 1937 Size: 773 612 550 4594 Fragments arranged by size: 4594 773 612 550 Acc65I G'GTAC_C Cuts at: 3850 3850 Size: 6529 AclI AA'CG_TT Cuts at: 1563 4656 5029 1563 Size: 3093 373 3063 Fragments arranged by size: 3093 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2703 4592 5976 6244 1632 Size: 1071 1889 1384 268 1917 Fragments arranged by size: 1917 1889 1384 1071 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6332 AflIII A'CryG_T Cuts at: 1203 2611 5428 6405 1203 Size: 1408 2817 977 1327 Fragments arranged by size: 2817 1408 1327 977 AgeI A'CCGG_T Cuts at: 5360 5360 Size: 6529 AhdI GACnn_n'nnGTC Cuts at: 5258 5258 Size: 6529 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6529 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6529 AlwNI CAG_nnn'CTG Cuts at: 4272 5844 4272 Size: 1572 4957 ApaLI G'TGCA_C Cuts at: 4586 5742 4586 Size: 1156 5373 ApoI r'AATT_y Cuts at: 1493 2284 2462 2477 2641 3094 3647 3838 4064 Size: 791 178 15 164 453 553 191 226 Cuts at: 4064 4216 6325 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 791 553 453 226 191 178 164 152 15 AseI AT'TA_AT Cuts at: 160 1032 5083 160 Size: 872 4051 1606 Fragments arranged by size: 4051 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6529 AvaI C'yCGr_G Cuts at: 1870 1943 2051 3854 1870 Size: 73 108 1803 4545 Fragments arranged by size: 4545 1803 108 73 BamHI G'GATC_C Cuts at: 2548 3859 4226 2548 Size: 1311 367 4851 Fragments arranged by size: 4851 1311 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2047 3850 5305 618 Size: 325 217 627 260 1803 1455 1842 Fragments arranged by size: 1842 1803 1455 627 325 260 217 BanII G_rGCy'C Cuts at: 1391 2418 3848 1391 Size: 1027 1430 4072 Fragments arranged by size: 4072 1430 1027 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2533 2826 928 Size: 971 634 293 4631 Fragments arranged by size: 4631 971 634 293 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4720 4754 1903 Size: 34 2783 34 3678 Fragments arranged by size: 3678 2783 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4421 5631 4421 Size: 1210 5319 BclI T'GATC_A Cuts at: 1548 1548 Size: 6529 BfrBI ATG'CAT Cuts at: 2932 3821 2932 Size: 889 5640 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2039 5140 136 Size: 107 122 71 631 605 367 3101 1525 Fragments arranged by size: 3101 1525 631 605 367 122 107 71 BglII A'GATC_T Cuts at: 2374 6524 2374 Size: 4150 2379 BlpI GC'TnA_GC Cuts at: 3946 3946 Size: 6529 Bme1580I G_kGCm'C Cuts at: 1790 2052 2294 2852 4590 5746 1790 Size: 262 242 558 1738 1156 2573 Fragments arranged by size: 2573 1738 1156 558 262 242 BmgBI CAC'GTC Cuts at: 1318 2614 3278 1318 Size: 1296 664 4569 Fragments arranged by size: 4569 1296 664 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4260 5218 442 Size: 416 53 83 399 24 2843 958 1753 Fragments arranged by size: 2843 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6529 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2316 3453 5189 1239 Size: 1077 1137 1736 2579 Fragments arranged by size: 2579 1736 1137 1077 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4654 5519 5817 4654 Size: 865 298 5366 Fragments arranged by size: 5366 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5192 882 Size: 617 3693 2219 Fragments arranged by size: 3693 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2410 3008 3826 4320 493 Size: 711 1206 598 818 494 2702 Fragments arranged by size: 2702 1206 818 711 598 494 BsaBI GATnn'nnATC Cuts at: 3525 3538 6523 3525 Size: 13 2985 3531 Fragments arranged by size: 3531 2985 13 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2031 4336 4718 5353 Size: 53 83 186 936 498 2305 382 635 Cuts at: 5353 275 Size: 1451 Fragments arranged by size: 2305 1451 936 635 498 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3456 4962 5360 5634 5781 6192 3456 Size: 1506 398 274 147 411 3793 Fragments arranged by size: 3793 1506 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2598 2628 1178 Size: 30 165 30 1195 30 5079 Fragments arranged by size: 5079 1195 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3171 3225 1186 Size: 1985 54 4490 Fragments arranged by size: 4490 1985 54 BseYI C'CCAG_C Cuts at: 5732 6228 5732 Size: 496 6033 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5956 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3979 4740 5768 1540 Size: 275 186 1978 761 1028 2301 Fragments arranged by size: 2301 1978 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2418 4590 4675 5746 2418 Size: 2172 85 1071 3201 Fragments arranged by size: 3201 2172 1071 85 BsmI GAATG_Cn' Cuts at: 4040 4133 4040 Size: 93 6436 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2294 2418 2852 3848 4590 4675 Size: 399 262 242 124 434 996 742 85 Cuts at: 4675 5746 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 996 742 434 399 262 242 124 85 BspEI T'CCGG_A Cuts at: 6192 6192 Size: 6529 BspHI T'CATG_A Cuts at: 2962 4418 6148 2962 Size: 1456 1730 3343 Fragments arranged by size: 3343 1730 1456 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2088 3884 844 Size: 1244 1796 3489 Fragments arranged by size: 3489 1796 1244 BsrBI CCG'CTC Cuts at: 1394 1823 4416 1394 Size: 429 2593 3507 Fragments arranged by size: 3507 2593 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2576 3192 3342 5024 5198 65 Size: 1183 1328 616 150 1682 174 1396 Fragments arranged by size: 1682 1396 1328 1183 616 174 150 BsrFI r'CCGG_y Cuts at: 1700 1810 2705 5173 5360 1700 Size: 110 895 2468 187 2869 Fragments arranged by size: 2869 2468 895 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6529 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6529 BssSI C'ACGA_G Cuts at: 4589 5601 4589 Size: 1012 5517 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6529 BstBI TT'CG_AA Cuts at: 2007 5333 2007 Size: 3326 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6529 BstYI r'GATC_y Cuts at: 1972 2374 2548 3365 3389 3859 4226 4611 4628 Size: 402 174 817 24 470 367 385 17 Cuts at: 4628 6080 6166 6178 6524 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 817 470 402 385 367 346 174 86 24 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2108 2127 3238 513 Size: 552 957 86 19 1111 3804 Fragments arranged by size: 3804 1111 957 552 86 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3260 487 Size: 683 56 2034 3756 Fragments arranged by size: 3756 2034 683 56 BtsI GCAGTG_nn' Cuts at: 3337 4045 4839 4859 3337 Size: 708 794 20 5007 Fragments arranged by size: 5007 794 708 20 ClaI AT'CG_AT Cuts at: 1554 3519 1554 Size: 1965 4564 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2710 2745 529 Size: 35 2146 35 4313 Fragments arranged by size: 4313 2146 35 35 DraI TTT'AAA Cuts at: 3833 3833 Size: 6529 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5536 809 Size: 507 4220 1802 Fragments arranged by size: 4220 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3217 3976 4865 Size: 54 1425 50 275 18 1387 759 889 Cuts at: 4865 6494 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1387 889 759 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3976 1537 Size: 275 2164 4090 Fragments arranged by size: 4090 2164 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2202 2961 4459 1353 Size: 849 759 1498 3423 Fragments arranged by size: 3423 1498 849 759 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 2150 5116 5490 5636 387 Size: 1064 138 453 108 2966 374 146 1280 Fragments arranged by size: 2966 1280 1064 453 374 146 138 108 EcoICRI GAG'CTC Cuts at: 2416 2416 Size: 6529 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2316 2703 3453 4592 5189 5976 6244 Size: 393 684 387 750 1139 597 787 268 Cuts at: 6244 1239 Size: 1524 Fragments arranged by size: 1524 1139 787 750 684 597 393 387 268 EcoRI G'AATT_C Cuts at: 2284 2462 3838 4216 2284 Size: 178 1376 378 4597 Fragments arranged by size: 4597 1376 378 178 FspI TGC'GCA Cuts at: 5035 5035 Size: 6529 HaeII r_GCGC'y Cuts at: 1678 5676 1678 Size: 3998 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2598 2612 2630 2644 2936 Size: 32 135 32 1026 14 18 14 292 Cuts at: 2936 2968 5170 5202 5244 5276 1373 Size: 32 2202 32 42 32 2626 Fragments arranged by size: 2626 2202 1026 292 135 42 32 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 3873 4119 669 Size: 988 281 1935 246 3079 Fragments arranged by size: 3079 1935 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6529 HpaI GTT'AAC Cuts at: 4119 4119 Size: 6529 KpnI G_GTAC'C Cuts at: 3854 3854 Size: 6529 MfeI C'AATT_G Cuts at: 2871 4128 2871 Size: 1257 5272 MluI A'CGCG_T Cuts at: 6405 6405 Size: 6529 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2738 5643 5827 1305 Size: 1433 2905 184 2007 Fragments arranged by size: 2905 2007 1433 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4707 Fragments arranged by size: 4707 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3026 3521 4487 4846 5005 518 Size: 648 1860 495 966 359 159 2042 Fragments arranged by size: 2042 1860 966 648 495 359 159 MspA1I CmG'CkG Cuts at: 1606 2110 2129 3746 3945 4622 5770 6015 1606 Size: 504 19 1617 199 677 1148 245 2120 Fragments arranged by size: 2120 1617 1148 677 504 245 199 19 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5020 Fragments arranged by size: 5020 957 552 NdeI CA'TA_TG Cuts at: 387 2347 387 Size: 1960 4569 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6529 NotI GC'GGCC_GC Cuts at: 3976 3976 Size: 6529 NsiI A_TGCA'T Cuts at: 2934 3823 2934 Size: 889 5640 NspI r_CATG'y Cuts at: 1132 1229 2936 3887 5432 1132 Size: 97 1707 951 1545 2229 Fragments arranged by size: 2229 1707 1545 951 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6529 PciI A'CATG_T Cuts at: 5428 5428 Size: 6529 PflMI CCAn_nnn'nTGG Cuts at: 1108 3134 1108 Size: 2026 4503 PfoI T'CCnGG_A Cuts at: 2602 2602 Size: 6529 PmeI GTTT'AAAC Cuts at: 3833 3833 Size: 6529 PmlI CAC'GTG Cuts at: 3008 3008 Size: 6529 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4598 4630 6111 6143 4598 Size: 32 1481 32 4984 Fragments arranged by size: 4984 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6529 PsiI TTA'TAA Cuts at: 4099 4099 Size: 6529 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2701 2733 2701 Size: 32 6497 PstI C_TGCA'G Cuts at: 830 2085 3761 3881 830 Size: 1255 1676 120 3478 Fragments arranged by size: 3478 1676 1255 120 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6529 PvuII CAG'CTG Cuts at: 3746 3746 Size: 6529 SacI G_AGCT'C Cuts at: 2418 2418 Size: 6529 SacII CC_GC'GG Cuts at: 2111 2130 2111 Size: 19 6510 SalI G'TCGA_C Cuts at: 1936 3871 1936 Size: 1935 4594 SbfI CC_TGCA'GG Cuts at: 3881 3881 Size: 6529 ScaI AGT'ACT Cuts at: 4777 6489 4777 Size: 1712 4817 SexAI A'CCwGG_T Cuts at: 3487 3487 Size: 6529 SfcI C'TryA_G Cuts at: 826 1045 2081 2235 3757 3877 5012 5693 5884 Size: 219 1036 154 1522 120 1135 681 191 Cuts at: 5884 826 Size: 1471 Fragments arranged by size: 1522 1471 1135 1036 681 219 191 154 120 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6529 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6529 SmaI CCC'GGG Cuts at: 1872 2053 3856 1872 Size: 181 1803 4545 Fragments arranged by size: 4545 1803 181 SmlI C'TyrA_G Cuts at: 820 1017 1943 4633 5534 5796 820 Size: 197 926 2690 901 262 1553 Fragments arranged by size: 2690 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3826 4320 493 Size: 3333 494 2702 Fragments arranged by size: 3333 2702 494 SpeI A'CTAG_T Cuts at: 152 3251 152 Size: 3099 3430 SphI G_CATG'C Cuts at: 1132 3887 1132 Size: 2755 3774 SspI AAT'ATT Cuts at: 5 52 2539 4453 5 Size: 47 2487 1914 2081 Fragments arranged by size: 2487 2081 1914 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6529 StyI C'CwwG_G Cuts at: 513 1065 2022 3968 513 Size: 552 957 1946 3074 Fragments arranged by size: 3074 1946 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4556 4573 4726 4911 5356 325 Size: 1255 2976 17 153 185 445 1498 Fragments arranged by size: 2976 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4775 6487 96 Size: 274 80 33 51 1443 2798 1712 138 Fragments arranged by size: 2798 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6489 6521 6489 Size: 32 6497 Tth111I GACn'n_nGTC Cuts at: 1316 3036 1316 Size: 1720 4809 XbaI T'CTAG_A Cuts at: 3865 3865 Size: 6529 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6529 XmaI C'CCGG_G Cuts at: 1870 2051 3854 1870 Size: 181 1803 4545 Fragments arranged by size: 4545 1803 181 XmnI GAAnn'nnTTC Cuts at: 2880 4658 2880 Size: 1778 4751 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4337 276 Size: 53 83 186 936 2803 2468 Fragments arranged by size: 2803 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI RsrII SanDI SapI SfiI SfoI SrfI SwaI XcmI